1. An Extract from Wax Apple (Syzygium samarangense (Blume) Merrill and Perry) Effects Glycogenesis and Glycolysis Pathways in Tumor Necrosis Factor-α-Treated FL83B Mouse Hepatocytes
- Author
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Wen Chang Chang, Chiao Li Chang, and Szu Chuan Shen
- Subjects
glycogenesis ,glucose metabolism ,Syzygium ,lcsh:TX341-641 ,Carbohydrate metabolism ,Article ,Cell Line ,chemistry.chemical_compound ,Mice ,insulin resistance ,wax apple ,Animals ,Glycolysis ,Glycogen synthase ,Hexokinase ,Nutrition and Dietetics ,biology ,Glycogen ,Plant Extracts ,Tumor Necrosis Factor-alpha ,Metabolism ,glycolysis ,Glycogen Synthase ,chemistry ,Biochemistry ,Glycogenesis ,FL83B mouse hepatocytes ,Fruit ,biology.protein ,Hepatocytes ,lcsh:Nutrition. Foods and food supply ,Food Science ,Phosphofructokinase - Abstract
FL83B mouse hepatocytes were treated with tumor necrosis factor-α (TNF-α) to induce insulin resistance to investigate the effect of a wax apple aqueous extract (WAE) in insulin-resistant mouse hepatocytes. The uptake of 2-[N-(7-nitrobenz-2-oxa-1, 3-diazol-4-yl)amino]-2-deoxyglucose (2 NBDG), a fluorescent d-glucose derivative, was performed, and the metabolism of carbohydrates was evaluated by examining the expression of glycogenesis or glycolysis-related proteins in insulin-resistant hepatocytes. The results show that WAE significantly improves the uptake of glucose and enhances glycogen content in insulin-resistant FL83B mouse hepatocytes. The results from Western blot analysis also reveal that WAE increases the expression of glycogen synthase (GS), hexokinase (HXK), glucose-6-phosphate dehydrogenase (G6PD), phosphofructokinase (PFK) and aldolase in TNF-α treated cells, indicating that WAE may ameliorate glucose metabolism by promoting glycogen synthesis and the glycolysis pathways in insulin-resistant FL83B mouse hepatocytes.
- Published
- 2013