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Objective To determine whether the performance of a new quantum dots–based point-of-care test (POCT) devices is qualified for procalcitonin testing. Methods Finger-prick and venous blood specimens from 153 patients were measured with a quantum dots–based POCT device; the results were compared with those from the reference method. Results The quantum dots–based POCT device correlated well with the reference method in measuring plasma, venous whole blood, and finger-prick blood. No significant bias was observed (−0.08 ng/mL). At 0.5 ng per mL cutoff value, the concordances were 96.6%, 94.6%, and 90.5% for plasma, venous whole blood, and finger-prick blood, respectively. And at 2 ng per mL cutoff value, the concordances were 98.0%, 96.6%, and 95.3%, respectively. Conclusions The quantum dots–based POCT device measured procalcitonin with multiple specimen types, high sensitivity, wide detection range, and short turnaround time. It would allow a more widespread use of procalcitonin and help lessen the burden of overcrowding in healthcare facilities in China. [ABSTRACT FROM AUTHOR]

Aim: The current study's aim was to assess gingival crevicular blood as a noninvasive method to measure blood glucose levels., Materials and Methods: The current study comprised a total of 50 patients who had been diagnosed with chronic periodontitis and age was ≥30 years old. The study's procedures were carried out after receiving informed consent. For finger capillary blood collection method, a sterile lancet was used to prick the finger and a drop of blood was taken, for gingival crevicular blood collection method, blood was taken from the gingival margin of the chosen site, and for venous blood collection method with the aid of a disposable syringe, a venous blood sample was taken from the patient's antecubital fossa for determining blood glucose levels. One-way analysis of variance (ANOVA) was used to examine the differences between the three methodologies' significance, and Karl Pearson's correlation ( r ) was used to determine their correlation., Results: The maximum glucose level was found in venous blood (187.78 ± 18.23), followed by finger capillary blood (181.88 ± 21.67) and gingival crevicular blood (169.04 ± 11.24). And there was no significant difference between the different blood collection methods ( p > 0.05). The positive significant correlation was found between gingival crevicular blood and finger capillary blood ( r = 0.912, p < 0.001). Correlation with gingival crevicular blood and venous blood showed a positive correlation ( r = 0.898, p < 0.001). Correlation between venous blood and finger capillary blood also showed a strong positive correlation ( r = 0.988, p < 0.001)., Conclusion: In conclusion, the findings of the current study suggest that blood drawn from the gingival crevicular during a clinical examination may be a great source for glucometric analysis. The gingival crevicular blood may show to be a promising technique for routine dental office screening for diabetes mellitus in periodontal patients, even if capillary/venous blood samples used for diabetes mellitus screening are the gold standard., Clinical Significance: Oral health is crucial for the early detection of many systemic disorders. As a result, dentists are crucial in the screening for systemic disorders. One of the prevalent chronic disorders is diabetes. Any systemic disease that is detected early enough can avoid long-term problems.

Abstract: Background: We present a prototype handheld device based on a newly developed optomagnetic technology for the sensitive detection of cardiac troponin I (cTnI) in a finger-prick blood sample with a turnaround time of 5min. Methods: The test was completed in a compact plastic disposable with on-board dry reagents and superparamagnetic nanoparticles. In our one-step assay, all reaction processes were precisely controlled using electromagnets positioned above and below the disposable. Nanoparticle labels (500nm) bound to the sensor surface via a sandwich immunoassay were detected using the optical technique of frustrated total internal reflection. Results: A calibration function measured in plasma demonstrates a limit of detection (mean of blank plus 3-fold the standard deviation) of 0.03ng/mL cTnI. A linear regression analysis of the region 0.03–6.5ng/mL yields a slope of 37±4, and a linear correlation coefficient of R 2 =0.98. The measuring range could be extended substantially to 100ng/mL by simultaneously imaging a second spot with a lower antibody concentration. Conclusions: The combination of magnetic particles and their fine actuation with electromagnets permits the rapid and sensitive detection of cTnI. Because of the potential high analytical performance and ease-of-use of the test, it is well suited for demanding point-of-care diagnostic applications. [Copyright &y& Elsevier]