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219 results on '"FILOSA, SILVANA"'

8. Role of apoptosis and Fas/FasL system in the oogenesis of the spotted rayTorpedo marmorata

Author

Filosa Silvana, Ricchiari Loredana, Motta Chiara Maria, Liguoro Annamaria, D'onghia Barbara, Andreuccetti Piero, Prisco Marina, Tammaro Stefania, Ricchiari, Loredana, D'Onghia, B, Liguoro, A, Tammaro, Stefania, Motta, CHIARA MARIA, Filosa, Silvana, Andreuccetti, Piero, Prisco, Marina, Ricchiari, L., B., D'Onghia, Am, Liguoro, S., Tammaro, S., Filosa, M., Prisco, D'Onghia, B., Tammaro, S., and Prisco, M.

Subjects
Fas Ligand Protein, animal structures, Apoptosis, Biology, Torpedo, Oogenesis, law.invention, Ovarian Follicle, law, In Situ Nick-End Labeling, Genetics, medicine, Animals, Sexual maturity, fas Receptor, Membrane Glycoproteins, TUNEL assay, cartilagineous fish, Oocyte selection, Cell Biology, Oocyte, apoptosi, Cell biology, medicine.anatomical_structure, Immunology, Oocytes, Female, oogenesi, fas/fasL, Germ cell, Developmental Biology
Abstract

In the present paper we investigated the role played by apoptosis during oogenesis in the cartilagineous fish Torpedo marmorata. TEM, TUNEL and immunohistochemical techniques were employed to specifically reveal morphological and biochemical hallmarks of apoptosis in specimens from birth to sexual maturity. Data obtained demonstrate that apoptosis occurs in prefollicular oocyte selection, in maintaining the homeostasis of granulosa in healthy growing oocyte and in resorbing atretic follicles. In this respect, the involvement of apoptosis in Torpedo marmorata oogenesis closely parallels that found in mammals, thus confirming that strategies of germ cell selection among vertebrates have been evolutively preserved. Mol. Reprod. Dev. 66: 54–59, 2003. © 2003 Wiley-Liss, Inc.

Published
2003
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12. Biologia dello sviluppo

Author

ANDREUCCETTI, PIERO, FILOSA, SILVANA, Carnevali O., Dini L., Falugi C., Kalthoff K., Viscuso R., Andreuccetti, Piero, Carnevali, O., Dini, L., Falugi, C., Filosa, Silvana, Kalthoff, K., and Viscuso, R.

Published
2009

15. Le cellule follicolari di Podarcis sicula> un buon modello per lo studio del differenziamento cellulare

Author

MOTTA, CHIARA MARIA, SIMONIELLO, PALMA, FILOSA, SILVANA, Motta, CHIARA MARIA, Simoniello, Palma, and Filosa, Silvana

Subjects
rettili, modello biologico, cellule follicolari
Abstract

Il lavoro descrive le caratteristiche delle cellule follicolari del rettile Podarcis ed illustra come queste possano costituire un buon modello sperimentale per lo studio dei meccanismi di proliferazione, differenziamento e morte clelulare.

Published
2008

17. Follicle cell differentiation in the wall lizard Podarcis sicula

Author

MOTTA, CHIARA MARIA, SIMONIELLO, PALMA, TAMMARO, STEFANIA, FILOSA, SILVANA, non noto, Motta, CHIARA MARIA, Simoniello, Palma, Tammaro, Stefania, and Filosa, Silvana

Subjects
stomatognathic system, Fas and Fas-L, pyriform cells, caspase 3, otorhinolaryngologic diseases, apoptosis, DNase I, pyriform cell, apoptosi
Abstract

In Podarcis, undifferentiated, pre-differentiated and differentiated follicle cells can be recognised by shape, size, position and function. Undifferentiated (stromal) and pre-differentiated (small) cells are stem cells destined to proliferate and differentiate while differentiated cells (pyriforms) are nurse cells programmed to sustain oocyte growth and then degenerate at the end of previtellogenesis. The present work clarifies whether competence to undergo apoptosis in pyriforms is acquired at the time they originate from the small cells or later during aging. For this purpose the expression of Fas, Fas-L, caspase 3 and DNase I have been investigated by immunocytochemistry in small and pyriform cells. Results indicate that all these cell death factors are present exclusively in pyriforms. Their synthesis starts as soon as cells differentiate and that while aging, Fas and Fas-L are down and up regulated respectively. We conclude that pyriforms are committed for apoptosis since differentiation and that death occurs via mechanisms alternative to receptor or mitochondrial mediated pathways.

Published
2008

23. Effetti citotossici del cadmio in Danio rerio

Author

TAMMARO, STEFANIA, SIMONIELLO, PALMA, MOTTA, CHIARA MARIA, FILOSA, SILVANA, Frezza V., Tammaro, Stefania, Simoniello, Palma, Motta, CHIARA MARIA, Frezza, V., and Filosa, Silvana

Published
2006

29. Cadmium contaminated soil affects retinogenesis in lizard embryos

Author

Simoniello, Palma, Trinchella, Francesca, Filosa, Silvana, Scudiero, Rosaria, Magnani, Dario, Theil, Thomas, and Motta, Chiara Maria

Subjects
Neurons, Animals, Cadmium, Cell Differentiation, Gene Expression Regulation, Developmental, Lizards, RNA, Messenger, Retina, Soil Pollutants, Animal Science and Zoology, Ecology, Evolution, Behavior and Systematics, Genetics, Physiology, Molecular Biology, Medicine (all), Ecology, Evolution, Messenger, Gene Expression Regulation, Behavior and Systematics, RNA, Developmental, sense organs
Published
2014

39. Aspartic proteinases and vitellogenesis in oviparous vertebrates

Author

SCUDIERO, ROSARIA, FILOSA, SILVANA, DE STASIO R, BORRELLI L, RIGGIO M., S.G. PANDALAI ED, Scudiero, Rosaria, R., DE STASIO, L., Borrelli, Filosa, Silvana, M., Riggio, Pandalai S.G., DE STASIO, R, Borrelli, L, and Riggio, M.

Subjects
Aspartic proteinases, VITELLOGENESIS
Published
2002

40. Analisi comparativa degli otoliti in due specie di Nototenidi antartici: T. bernacchii e C. hamatus

Author

AVALLONE, BICE, MOTTA, CHIARA MARIA, TAMMARO, STEFANIA, Casciello M.G., BALSAMO, GIUSEPPE, MARMO, FRANCESCO, BALASSONE, GIUSEPPINA, FILOSA, SILVANA, Dipartimento di Ecologia-Università della Calabria, Dipartimento di Biologia Animale ed Ecologia Marina Università degli studi di Messina, Avallone, Bice, Motta, CHIARA MARIA, Tammaro, Stefania, Casciello, M. G., Balsamo, Giuseppe, Marmo, Francesco, Balassone, Giuseppina, and Filosa, Silvana

Published
2002

44. New site of temporary storage of rRNA in O. mykiss previtellogenic oocytes

Author

FUSCO S, IACOVIELLO R, INDOLFI S, TAMMARO S, MOTTA, CHIARA MARIA, FILOSA, SILVANA, Fusco, S, Filosa, Silvana, Iacoviello, R, Indolfi, S, Tammaro, Stefania, Motta, CHIARA MARIA, and Tammaro, S

Subjects
storage, trout, RNA, Ribosomal, Oncorhynchus mykiss, Vitellogenesis, Oocytes, Animals, Female, rRNA, oocyte
Abstract

This article describes a new organelle found in the cytoplasm of the growth stage fish oocytes. In particular, we describe its organization at the morphological level and investigate its composition by different cytochemical and immunocytochemical approaches with both light and electron microscope. The conclusion is that the body is a peculiar protein scaffold functioning as a temporary trap for the storage of rRNA in the mid to late growth stage oocytes. Its presence would be related to the reorganization of the mass of amplified rDNA in micronucleoli and to the consequent temporary stop in the rRNA synthesis.

Published
2000

45. Seasonal dependent effects of temperature on the response of adenylate cyclase to FSH stimulation in the oviparous lizard Podarcis sicula

Author

BORRELLI, LUCIA, DE STASIO, ROBERTA, MOTTA, CHIARA MARIA, FILOSA, SILVANA, PARISI E, Borrelli, S, DE STASIO, R, Motta, CHIARA MARIA, Parisi, E, Filosa, Silvana, Borrelli, Lucia, and DE STASIO, Roberta

Abstract

The study of environmental factors affecting vertebrate reproduction has long interested both developmental and evolutionary biologists. Although photoperiod has been considered to be an important environmental parameter for vertebrates such as birds, temperature is probably a primary external factor responsible for reproductive cyclicity in reptiles. In spite of the progress made in the understanding of reptilian reproductive strategies and adaptations, much remains to be learned about the interplay between endocrine physiological factors, such as hormones, and environmental parameters. In this report, we have examined the effects of in vivo administered FSH on oocyte recruitment during the most significant periods of the reproductive cycle of the lizard, Podarcis sicula. The results show that when FSH is administered in proximity to the reproductive period, it stimulates oocyte growth and ovulation; when the hormone is administered at the beginning of the winter stasis it affects ovarian activity without inducing ovulation. Ovarian adenylate cyclase activity is moderately sensitive to in vitro FSH stimulation during the pre- and post-reproductive periods. The sensitivity to hormone stimulation increases significantly during the reproductive period and winter stasis. We have also tested the hypothesis that environmental temperature affects the responsiveness of ovarian adenylate cyclase to FSH stimulation. For such a purpose, we exposed animals to 28 degrees C or 4 degrees C in different periods of the ovarian cycle. The results show that, whenever the temperature applied mimics the thermal regime of the coming season, adenylate cyclase sensitivity to FSH shifts towards levels that anticipate the natural responsiveness.

Published
2000

46. Regulatory peptides and physiological adaptations to the cold environment in Antarctic teleosts

Author

Uva BM, Vallarino M, Tagliafierro G, Pestarino M, Falugi C, Mandich A, Masini MA, Sturla MA, Sturla M, Prato P, Candiani S, Fusco S., FILOSA, SILVANA, CAMPANELLA, CHIARA, MOTTA, CHIARA MARIA, Uva, Bm, Vallarino, M, Tagliafierro, G, Pestarino, M, Falugi, C, Mandich, A, Masini, Ma, Sturla, Ma, Sturla, M, Prato, P, Candiani, S, Filosa, Silvana, Campanella, Chiara, Motta, CHIARA MARIA, and Fusco, S.

Subjects
reproduction, regulatory peptide, antarctic teleost, brain, osmoregulation, intestine
Published
2000

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