1. Systemic microvascular endothelial dysfunction and disease severity in COVID-19 patients: Evaluation by laser Doppler perfusion monitoring and cytokine/chemokine analysis
- Author
-
Fabiana Muccillo, Vanessa Estato, Hugo C. Castro-Faria-Neto, Andrea De Lorenzo, Leticia R Sabioni, Eduardo Tibiriçá, and Cristiane da Cruz Lamas
- Subjects
0301 basic medicine ,Male ,medicine.medical_treatment ,Hemodynamics ,030204 cardiovascular system & hematology ,Severity of Illness Index ,Biochemistry ,M-CSF, macrophage colony-stimulating factor ,TRAIL, TNF-related apoptosis inducing ligand ,CCL2/MCP-1, monocyte chemoattractant protein-1 ,0302 clinical medicine ,Laser-Doppler Flowmetry ,S-COVID, severe COVID-19 ,Endothelial dysfunction ,IL, interleukins ,COVID-19, coronavirus disease 2019 ,Immunoassay ,TNF, tumor necrosis factor ,LIF, leukemia inhibitory factor ,IL-12p40, IL-12 subunit p70 ,Middle Aged ,PDGF-BB, platelet-derived growth factor ,VEGF, vascular endothelial growth factor ,Pathophysiology ,Healthy Volunteers ,Perfusion ,Cytokine ,SCGF- β, steam cell growth factor-β ,Cardiology ,Cytokines ,Female ,GM-CSF, granulocyte-monocyte colony-stimulating factor ,SDF-1α, stromal cell-derived factor-1α ,medicine.symptom ,Chemokines ,β-NGF, nerve growth factor ,Cardiology and Cardiovascular Medicine ,Mers-CoV, middle east respiratory syndrome coronavirus ,CCL5/RANTES, regulated upon activation normal Tcell expressed and secreted ,Adult ,medicine.medical_specialty ,IL-1Ra, IL-1 receptor antagonist ,LTH, local thermal hyperemia ,Inflammation ,GRO-α, growth-related oncogene-α ,MIG, monokine induced by interferon-γ ,CCL7/MCP-3, monocyte-specific chemokine-3 ,IL-2Rα, soluble IL-2 receptor α ,SARS-CoV-2, severe acute respiratory syndrome coronavirus 2 ,Article ,FGF basic, basic fibroblast growth factor ,IL-12p40, IL-12 subunit p40 ,Proinflammatory cytokine ,Microcirculation ,03 medical and health sciences ,LDPM, laser Doppler perfusion monitoring ,M-COVID, mild to moderate COVID-19 ,RT-PCR, reverse transcription polymerase chain reaction ,Internal medicine ,medicine ,MIF, migration inhibitory factor ,Humans ,IFN, interferon ,Aged ,Proinflammatory cytokines ,business.industry ,SCF, stem cell factor ,CXCL10/IP-10, IFN-γ-inducible protein-10 ,COVID-19 ,Cell Biology ,medicine.disease ,CCL247CTACK, cutaneous T cell-attracting chemokine ,G-CSF, granulocyte colony-stimulating factor ,030104 developmental biology ,CCL4/MIP-1β, macrophage inflammatory protein-1β ,Endothelium, Vascular ,CCL3/MIP-1α, human macrophage inflammatory protein-1- α ,business ,Laser Doppler perfusion monitoring - Abstract
Background Microvascular dysfunction, serum cytokines and chemokines may play important roles in pathophysiology of coronavirus disease 2019 (COVID-19), especially in severe cases. Methods Patients with COVID-19 underwent non-invasive evaluation of systemic endothelium-dependent microvascular reactivity - using laser Doppler perfusion monitoring in the skin of the forearm - coupled to local thermal hyperemia. Maximal microvascular vasodilatation (44 °C thermal plateau phase) was used as endpoint. A multiplex biometric immunoassay was used to assess a panel of 48 serum cytokines and chemokines. Severe COVID-19 (S-COVID) was defined according to WHO criteria, while all other cases of COVID-19 were considered mild to moderate (M-COVID). A group of healthy individuals who tested negative for SARS-CoV-2 served as a control group and was also evaluated with LDPM. Results Thirty-two patients with COVID-19 (25% S-COVID) and 14 controls were included. Basal microvascular flow was similar between M-COVID and controls (P = 0.69) but was higher in S-COVID than in controls (P = 0.005) and M-COVID patients (P = 0.01). The peak microvascular vasodilator response was markedly decreased in both patient groups (M-COVID, P = 0.001; S-COVID, P, Highlights • During acute COVID-19, endothelium-dependent microvascular vasodilator responses are reduced. • Impaired systemic microvascular vasodilation is more evident in severe vs mild-moderate COVID-19. • Increased levels of cytokines and chemokines are associated with COVID-19 severity.
- Published
- 2021
- Full Text
- View/download PDF