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Summary: Food safety is crucial globally, particularly regarding mycotoxins in food. Mycotoxins, primarily found in cereals, can also contaminate animal products. In Mexico, staple foods like maize and animal products may contain fumonisins, mycotoxins from Fusarium fungi linked to various health issues. Type B fumonisins (FBs) and their hydrolysis products are particularly toxic to humans. This study analysed the most commonly consumed foods in the Mexican diet, selected based on data from Mexican government institutions. Samples were processed and dried at various temperatures according to the food type. To identify the most effective extraction method, each sample underwent testing under different extraction conditions. The presence of FB1, FB2, FB3, and their hydrolysed forms in the selected foods, as well as their relative concentrations, was determined using high‐performance liquid chromatography coupled with quadrupole time‐of‐flight mass spectrometry (HPLC‐MS‐QTOF). Therefore, this work aims to analyse the presence of fumonisins as contaminants in foods consumed by Mexicans, providing an analytical method for their inspection. [ABSTRACT FROM AUTHOR]

Mycotoxins present a significant health concern within the animal-feed industry, with profound implications for the pig-farming sector. The objective of this study was to evaluate the efficacy of two commercial adsorbents, an organically modified clinoptilolite (OMC) and a multicomponent mycotoxin detoxifying agent (MMDA), to ameliorate the combined adverse effects of dietary aflatoxins (AFs: sum of AFB1, AFB2, AFG1, and AFG2), fumonisins (FBs), and zearalenone (ZEN) at levels of nearly 0.5, 1.0, and 1.0 mg/kg, on a cohort of cross-bred female pigs (N = 24). Pigs were randomly allocated into six experimental groups (control, mycotoxins (MTX) alone, MTX + OMC 1.5 kg/ton, MTX + OMC 3.0 kg/ton, MTX + MMDA 1.5 kg/ton, and MTX + MMDA 3.0 kg/ton), each consisting of four individuals, and subjected to a dietary regimen spanning 42 days. The administration of combined AFs, FBs, and ZEN reduced the body-weight gain and increased the relative weight of the liver, while there was no negative influence observed on the serum biochemistry of animals. The supplementation of OMC and MMDA ameliorated the toxic effects, as observed in organ histology, and provided a notable reduction in residual AFs, FBs, and ZEN levels in the liver and kidneys. Moreover, the OMC supplementation was able to reduce the initiation of liver carcinogenesis without any hepatotoxic side effects. These findings demonstrate that the use of OMC and MMDA effectively mitigated the adverse effects of dietary AFs, FBs, and ZEN in piglets. Further studies should explore the long-term protective effects of the studied adsorbent supplementation to optimize mycotoxin management strategies in pig-farming operations. [ABSTRACT FROM AUTHOR]

Mycotoxins present a significant health concern within the animal-feed industry, with profound implications for the pig-farming sector. The objective of this study was to evaluate the efficacy of two commercial adsorbents, an organically modified clinoptilolite (OMC) and a multicomponent mycotoxin detoxifying agent (MMDA), to ameliorate the combined adverse effects of dietary aflatoxins (AFs: sum of AFB1, AFB2, AFG1, and AFG2), fumonisins (FBs), and zearalenone (ZEN) at levels of nearly 0.5, 1.0, and 1.0 mg/kg, on a cohort of cross-bred female pigs (N = 24). Pigs were randomly allocated into six experimental groups (control, mycotoxins (MTX) alone, MTX + OMC 1.5 kg/ton, MTX + OMC 3.0 kg/ton, MTX + MMDA 1.5 kg/ton, and MTX + MMDA 3.0 kg/ton), each consisting of four individuals, and subjected to a dietary regimen spanning 42 days. The administration of combined AFs, FBs, and ZEN reduced the body-weight gain and increased the relative weight of the liver, while there was no negative influence observed on the serum biochemistry of animals. The supplementation of OMC and MMDA ameliorated the toxic effects, as observed in organ histology, and provided a notable reduction in residual AFs, FBs, and ZEN levels in the liver and kidneys. Moreover, the OMC supplementation was able to reduce the initiation of liver carcinogenesis without any hepatotoxic side effects. These findings demonstrate that the use of OMC and MMDA effectively mitigated the adverse effects of dietary AFs, FBs, and ZEN in piglets. Further studies should explore the long-term protective effects of the studied adsorbent supplementation to optimize mycotoxin management strategies in pig-farming operations.

Fusarium verticillioides, a well-known fungal pathogen that causes severe disease in maize and contaminates the grains with fumonisin B1 (FB1) mycotoxin, affects the yield and quality of maize worldwide. The intrinsic roles of peroxisome targeting signal (PTS)–containing proteins in phytopathogens remain elusive. We therefore explored the regulatory role and other biological functions of the components of PTS2 receptor complex, FvPex7 and FvPex20, in F. verticillioides. We found that FvPex7 directly interacts with the carboxyl terminus of FvPex20 in F. verticillioides. PTS2-containing proteins are recognized and bound by the FvPex7 receptor or the FvPex7-Pex20 receptor complex in the cytoplasm, but the peroxisome localization of the PTS2-Pex7-Pex20 complex is only determined by Pex20 in F. verticillioides. However, we observed that some putative PTS2 proteins that interact with Pex7 are not transported into the peroxisomes, but a PTS1 protein that interacts with Pex5 was detected in the peroxisomes. Furthermore, ΔFvpex7pex20 as well as ΔFvpex7pex5 double mutants exhibited reduced pathogenicity and FB1 biosynthesis, along with defects in conidiation. The PTS2 receptor complex mutants (ΔFvpex7pex20) grew slowly on minimal media and showed reduced sensitivity to cell wall and cell membrane stress-inducing agents compared to the wild type. Taken together, we conclude that the PTS2 receptor complex mediates peroxisome matrix proteins import and contributes to pathogenicity and FB1 biosynthesis in F. verticillioides. Key points: • FvPex7 directly interacts with FvPex20 in F. verticillioides. • vThe PTS2 receptor complex is essential for the importation of PTS2-containing matrix protein into peroxisomes in F. verticillioides. • Fvpex7/pex20 is involved in pathogenicity and FB1 biosynthesis in F. verticillioides. [ABSTRACT FROM AUTHOR]

The high osmolarity glycerol 1 mitogen-activated protein kinase (Hog1-MAPK) cascade genes are important for diverse biological processes. The activated Hog1 upon multiple environmental stress stimuli enters into the nucleus where it directly phosphorylates transcription factors to regulate various physiological processes in phytopathogenic fungi. However, their roles have not been well-characterized in Fusarium verticillioides. In this study, FvHog1 is identified and functionally analyzed. The findings reveal that the phosphorylation level and nuclear localization of FvHog1 are increased in Fumonisin B1 (FB1)-inducing condition to regulate the expression of FB1 biosynthesis FUM genes. More importantly, the deletion mutants of Hog1-MAPK pathway show increased sensitivity to Ca2+ stress and elevated intracellular Ca2+ content. The phosphorylation level and nuclear localization of FvHog1 are increased with Ca2+ treatment. Furthermore, our results show that FvHog1 can directly phosphorylate Ca2+-responsive zinc finger transcription factor 1 (FvCrz1) to regulate Ca2+ homeostasis. In conclusion, our findings indicate that FvHog1 is required for FB1 biosynthesis, pathogenicity and Ca2+ homeostasis in F. verticillioides. It provides a theoretical basis for effective prevention and control maize ear and stalk rot disease. [Display omitted] • FvHog1 is important for conidia, Fumonisin B1 (FB1) production and virulence in F. verticillioides. • The phosphorylated FvHog1 enters nucleus to regulate the expression of FB1 biosynthesis FUM genes. • FvHog1 is involved in various environmental stress response. • The deletion mutants of Hog1-MAPK pathway show increased sensitivity to Ca2+ stress and elevated intracellular Ca2+ content. • FvHog1 can directly phosphorylate Ca2+-responsive zinc finger transcription factor 1 (FvCrz1) to regulate Ca2+ homeostasis. [ABSTRACT FROM AUTHOR]

Fungi produce a series of toxic compounds on corn, especially Fumonisin B1 (FB1) toxin produced by Fusarium spp. and promoting cancer activity in humans and animals. This study aimed to the isolation and identification of fungi associated with local corn seeds and the detection for the presence of FB1 by using ELISA technique. Thirty samples of corn ears were collected from silos and markets in Baghdad city during the period from November 2018 to March 2019. The present study found that Fusarium was the dominant isolate among fungi in terms of the relative density 57.07%, followed by Aspergillus 31.17%, Rhizopus 3.36%, Alternaria 2.88%, Mucor 2.16%, Penicillium 1.92%, Trichothecium 0.96%, and Helminthosporium 0.48%. FB1 was detected in all samples of the silos and markets with a concentration range of 13.69 - 175.54 μg/kg. There were no significant differences in FB1concentration among samples collected from the silos and markets. Also, no relationship was found between the number of infected seeds by Fusarium spp. and FB1concentrations. [ABSTRACT FROM AUTHOR]

Fumonisin B1 is toxic secondary metabolites compound produced by Fusarium spp. on maize and maize products causes health problems to human and animal. Therefore, this research is planned to study the effect of FB1 on the expression of TLR-2 & 4 in liver and kidney cells of mice. Four group of male mice were orally administrated with single dose of FB1 toxin as the following: 0 ppb, 800 ppb, 1200 ppb and 1600 ppb. After two weeks all animals were sacrificed, liver and kidney autopsies were taken and the level of TLR-2 & 4 detected in each four group by immunohistochemistry technique (IHC). According to the IHC examination of groups (1, 2, 3 and 4) strong expression of TLR2 in liver and kidney were (0%, 33.3%, 100%, 100%), respectively. This result shows significant different between these groups P £ 0.01. The expression of TLR4 for these groups in liver was (0%, 0%, 33.3%, 66.6%). Respectively, and in kidney were (0%, 0%, 50%, 66.6%). Chi square was significantly difference between P £ 0.01 these groups. In conclusion, FB1 has a significant effect on TLR-2 and 4 expression in liver and kidney at high doses and the staining was found in cytoplasm and membrane of hepatocyte and kidney tubules. [ABSTRACT FROM AUTHOR]

Fumonisin B 1 is a mycotoxin produced by Fusarium verticillioides and Fusarium proliferatum found in various crops, particularly maize. Besides carcinogenicity, other manifestations have been registered in different animals and in humans. In the case of humans, epidemiological studies have reported high prevalence of esophageal cancer in populations exposed to fumonisins. This study aimed to evaluate the minimum concentration of FB 1 capable of inducing cytotoxicity (cell viability test), genotoxicity (comet assay) and mutagenicity (micronucleus) in cultured human leukocytes and to evaluate the effectiveness of in silico tests to predict FB 1 toxicity. All concentrations analyzed (200; 100; 50; 5; 0.5; 0.05; 0.005 μg/mL and 300; 30; 3; 1; 0.1; 0.01 fg/mL) except the lowest demonstrated dose-dependent toxicity in all parameters analyzed (p < 0.05 to p < 0.0001). As for predictions, only the Lazar software showed carcinogenicity of FB 1 for rats. Thus, it is evident that FB 1 is able to induce dose-dependent damage at low concentrations, and that computational tests, although desirable for prediction, are not effective as biological tests to determine toxicity, at least of FB 1 and within the experimental conditions tested. • Incidence of fumonisin B 1 in cultivars and food. • Fumonisin B 1 as a cause of disease in animals and humans. • Fumonisin B 1 causing cytotoxicity, mutagenicity and genotoxicity. • Toxicological predictions favor the structure-activity relationship of mycotoxins, especially fumonisin B 1. [ABSTRACT FROM AUTHOR]

Fusarium verticillioides is one of the important mycotoxigenic pathogens of maize since it causes severe yield losses and produces fumonisins (FBs) to threaten human and animal health. Previous studies showed that temperature and water activity (a w) are two pivotal environmental factors affecting F. verticillioides growth and FBs production during maize storage. However, the genome-wide transcriptome analysis of differentially expressed genes (DEGs) in F. verticillioides under the stress combinations of temperature and a w has not been studied in detail. In this study, DEGs of F. verticillioides and their related regulatory pathways were analyzed in response to the stress of temperature and a w combinations using RNA-Seq. The results showed that the optimal growth conditions for F. verticillioides were 0.98 a w and 25 °C, whereas the highest per-unit yield of the fumonisin B 1 (FB 1) was observed at 0.98 a w and 15 °C. The RNA-seq analysis showed that 9648 DEGs were affected by temperature regardless of a w levels, whereas only 218 DEGs were affected by a w regardless of temperature variations. Gene Ontology (GO) analysis revealed that a decrease in temperature at both a w levels led to a significant upregulation of genes associated with 24 biological processes, while three biological processes were downregulated. Furthermore, when a w was decreased at both temperatures, seven biological processes were significantly upregulated and four were downregulated. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis revealed that the genes, whose expression was upregulated when the temperature decreased, were predominantly associated with the proteasome pathway, whereas the genes, whose expression was downregulated when the a w decreased, were mainly linked to amino acid metabolism. For the FB 1 , except for the FUM15 gene, the other 15 biosynthetic-related genes were highly expressed at 0.98 a w and 15 °C. In addition, the expression pattern analysis of other biosynthetic genes involved in secondary metabolite production and regulation of fumonisins production was conducted to explore how this fungus responds to the stress combinations of temperature and a w. Overall, this study primarily examines the impact of temperature and a w on the growth of F. verticillioides and its production of FB 1 using transcriptome data. The findings presented here have the potential to contribute to the development of novel strategies for managing fungal diseases and offer valuable insights for preventing fumonisin contamination in food and feed storage. • Higher temperature (25 °C) and higher water activity (0.98 a w) favored the growth of Fusarium verticillioides • Lower temperature (15 °C) and higher water activity (0.98 a w) favored the fumonisin B 1 production in F. verticillioides • Secondary metabolite gene clusters were differentially expressed under temperature/water activity stresses. • The RNA-seq analysis provides gene groups for further investigation of fumonisin biosynthesis in F. verticillioides [ABSTRACT FROM AUTHOR]

ABSTRACT: The aim of the present study was to assess the occurrence of aflatoxins (AFs) and fumonisins (FBs) in feed ingredients (corn and soybean meal) and finishing feed in a broiler operation system, as well was to evaluate their effect on the productivity of 20 batches of broilers produced and the histology status of broilers’ liver after slaughter. Corn samples presented the highest frequencies of AFs and FBs, at mean levels of 29.1 and 2,100µg/kg, respectively. Soybean samples presented mean levels of 1.5 and 70µg/kg for AFs and FBs, respectively. Batches of broilers receiving feed containing FB levels higher than 1,000µg/kg had lower weight gain and higher mortality rates, while those fed rations with AFs equal or above the limit of quantification (LOQ) of the analytical method presented higher scores of histological changes in the liver. A dilution effect was observed for AFs and FBs from ingredients, especially corn, to feed during manufacture, whilst not enough to prevent losses in productivity. Results of this trial highlighted the need for strict control of mycotoxins in corn intended for broilers.

The aim of the present study was to assess the occurrence of aflatoxins (AFs) and fumonisins (FBs) in feed ingredients (corn and soybean meal) and finishing feed in a broiler operation system, as well was to evaluate their effect on the productivity of 20 batches of broilers produced and the histology status of broilers' liver after slaughter. Corn samples presented the highest frequencies of AFs and FBs, at mean levels of 29.1 and 2,100μg/kg, respectively. Soybean samples presented mean levels of 1.5 and 70μg/kg for AFs and FBs, respectively. Batches of broilers receiving feed containing FB levels higher than 1,000μg/kg had lower weight gain and higher mortality rates, while those fed rations with AFs equal or above the limit of quantification (LOQ) of the analytical method presented higher scores of histological changes in the liver. A dilution effect was observed for AFs and FBs from ingredients, especially corn, to feed during manufacture, whilst not enough to prevent losses in productivity. Results of this trial highlighted the need for strict control of mycotoxins in corn intended for broilers. [ABSTRACT FROM AUTHOR]

Although fumonisin B1 (FB1) is a mycotoxin poorly studied in wheat, the Fusarium genus fungi have been frequently detected in this cereal, especially in subtropical regions such as southern Brazil, thus studies on their occurrence are required. This study evaluated the mycobiota, water activity and the presence of FB1 in 11 wheat cultivars grown in the northeastern and northwestern of Rio Grande do Sul. The mycobiota was identified by morphological and molecular techniques and FB1 was determined by liquid chromatography with fluorescence detection (HPLC-FL). Microbial counts lower than 103CFU were found in the samples, and the fungi genera Phoma, Cladosporium and Fusarium were the most frequent. Despite this, 54% of the samples were contaminated with FB1 at levels ranging from 958 to 4,906µg FB1 kg-1.

Resumo:A fumonisina B1 (FB1) é um metabólito secundário produzido principalmente por Fusarium verticilioides em diversos tipos de alimentos, principalmente o milho, o qual constitui a base para composição de rações para várias espécies de animais domésticos. A FB1é particularmente tóxica para suínos, cujas manifestações clínicas são evidentes em animais expostos a altas concentrações de FB1 na ração (em geral, acima de 30mg/kg). No entanto, são escassos os estudos sobre os efeitos da FB1em suínos alimentados com rações contendo baixas concentrações de fumonisinas, as quais são mais prováveis de serem encontradas em condições de campo. O objetivo do estudo foi avaliar os efeitos da exposição de leitões a baixos níveis de FB1 na ração, durante 28 dias, sobre o ganho de peso, consumo de ração, peso relativo de órgãos e aspectos histológicos do baço, fígado, pulmões, rins e coração. Vinte e quatro leitões foram distribuídos em 4 grupos experimentais e alimentados com rações contendo 0mg (controle), 3,0mg, 6,0mg ou 9,0mg FB1/kg de ração. As diferentes dietas não afetaram (P>0,05) o ganho de peso e nem o peso relativo dos órgãos analisados. Não foram constatadas lesões macroscópicas ou histopatológicas no baço, fígado, rins e coração. No entanto, foram observadas lesões histopatológicas nos pulmões de todos os suínos alimentados com rações contaminadas com fumonisinas, indicando que nenhum dos níveis de FB1 usados no experimento poderia ser considerado como seguro para suínos. São necessários novos estudos sobre os mecanismos de ação tóxica da FB1 em suínos, sobretudo em condições de exposição prolongada a baixos níveis de contaminação na ração.

O objetivo do presente trabalho foi estudar o efeito da fumonisina B1 (FB1) sobre o peso corpóreo e as vísceras (fígado, rim e coração) de codorna poedeira japonesa (Coturnix coturnix japonica), devido ao escasso dado toxicológico nesta espécie. Quatro grupos, sendo cada um constituído de 32 codornas de linhagem comercial, receberam ração contendo FB1 nas concentrações de 0 (controle), 10, 50 e 250mg kg-1, durante 28 dias. Observou-se uma redução (P0,05), porém as aves do grupo 50 e 250mg kg-1 apresentaram aumento no peso relativo de fígado (PThis research was aimed at evaluating the effect of fumonisin B1 (FB1) on body weight and viscera (liver, kidney and heart) of laying Japanese quail (Coturnix coturnix japonica), due to the little toxicological data on this species. Four experimental groups of 32 commercial quails were designed and exposed to ingestion test with feed containing 0 (controls), 10, 50 or 250mg FB1 kg-1 feed, during 28 days. Birds of group 250mg FB1 kg-1 showed lower body weight (P0.05) among treatments. However, the relative weight of liver increased (P

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Fumonisin B1 (FB1) and ochratoxin A (OTA) are fungal metabolites of worldwide concern because of their effect on human and animal health, as both have been classified by IARC as possible carcinogens (Group 2B). Beetroot is a source of dietary fiber, folic acid, and vitamin C, and some studies have demonstrated their antioxidant activity. Therefore, this work presents the cytoprotective effect of beetroot extract (BRE) on a neuroblastoma cell line (SH-SY5Y cells) exposed to FB1, OTA, and its combination. Cytotoxicity was studied by the MTT ([3–4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay, for 24 h and 48 h. Simultaneous treatment and pre-treatment strategies were tested with 1:512–1:2 and 1:0 dilutions of BRE, with a concentration range from 0.4 to 100 μM of FB1 and from 0.19 to 50 μM of OTA. IC 50 values of 5.8 μM and 9.1 μM at 24 h and 48 h, respectively were obtained for OTA while no cytotoxic effect was detected at the concentrations tested for FB1. Cytoprotection with increased viability was obtained when the simultaneous BRE + OTA strategy was performed. Finally, better protection was observed in the pretreatment strategy in which cells were exposed 24 h previously to BRE, compared to that shown in the simultaneous assay. [Display omitted] • Cytotoxic effect of Ochratoxin A reached IC 50 values on SH-SY5Y cells. • Beetroot extract cytoprotects SH-SY5Y cell line exposed to OTA and FB1. • Pre-treatment strategy provided the greatest cytoprotection in SH-SY5Y cells. [ABSTRACT FROM AUTHOR]

Highlights: [•] Fumonisin B1 (FB1) was determined in 120 samples of corn products in São Paulo, Brazil. [•] The highest levels of FB1 were found in corn meal at a mean concentration of 474.6μgkg−1. [•] Mean PDI of FB1 estimated by FFQ and foods analyzed was nearly 3% of the PMTDI recommended. [•] The consumption data indicate a low human exposure to FB1 in the population studied. [ABSTRACT FROM AUTHOR]

Metacaspase is discovered gene family with distant caspase homologs in protozoa, fungi, and plants. The Arabidopsis genome has nine metacaspase genes. Based on the presence or absence of an N-terminal domain, they can be divided into two types of metacaspase genes: type I ( AtMC1 to 3) and type II ( AtMC4 to 9). To examine the expression patterns of the Arabidopsis metacaspase gene family, we measured the levels of expression of the metacaspase genes in various tissues and during biotic stresses by quantitative reverse transcription-polymerase chain reaction. As expected, the nine AtMC genes were expressed differently among tissues and during diverse stress conditions. In addition, to address the spatial expression of AtMC genes, their expression patterns using the promoter region of AtMCs (0.85 kb to 2.55 kb) were examined by the GUS reporter system. By observing GUS activity in transgenic plants containing nine AtMCpro::GUS constructs, we found that expression of these nine AtMC genes was regulated developmentally and spatially. We also observed that AtMC::green fluorescent fusion proteins were mainly localized in the nucleus using the Arabidopsis protoplast transient assay. In summary, our data suggest that AtMCs gene family be involved in various defense responses during biotic stresses. [ABSTRACT FROM AUTHOR]

Abstract: Three Fusarium species associated with bakanae disease of rice (Fusarium fujikuroi, Fusarium proliferatum, and Fusarium verticillioides) were investigated for their ability to produce fumonisins (FB1 and FB2) under different light conditions, and for pathogenicity. Compared to darkness, the conditions that highly stimulated fumonisin production were yellow and green light in F. verticillioides strains; white and blue light, and light/dark alternation in F. fujikuroi and F. proliferatum strains. In general, all light conditions positively influenced fumonisin production with respect to the dark. Expression of the FUM1 gene, which is necessary for the initiation of fumonisin production, was in accordance with the fumonisin biosynthetic profile. High and low fumonisin-producing F. fujikuroi strains showed typical symptoms of bakanae disease, abundant fumonisin-producing F. verticillioides strains exhibited chlorosis and stunting of rice plants, while fumonisin-producing F. proliferatum strains were asymptomatic on rice. We report that F. fujikuroi might be an abundant fumonisin producer with levels comparable to that of F. verticillioides and F. proliferatum, highlighting the need of deeper mycotoxicological analyses on rice isolates of F. fujikuroi. Our results showed for the first time the influence of light on fumonisin production in isolates of F. fujikuroi, F. proliferatum, and F. verticillioides from rice. [Copyright &y& Elsevier]

Background: U19/EAF2 is a potential tumor suppressor exhibiting frequent down-regulation and allelic loss in advanced human prostate cancer specimens. U19/EAF2 has also been identified as ELL-associated factor 2 (EAF2) based on its binding to ELL, a fusion partner of MLL in acute myeloid leukemia. U19/EAF2 is a putative transcription factor with a transactivation domain and capability of sequence-specific DNA binding.Methods: Yeast-two-hybrid-screening was used to identify U19/EAF2-binding partners. Co-immunoprecipitation and mammalian 1-hybrid assay were used to characterize a U19/EAF2-binding partner.Results: FB1, an E2A fusion partner in childhood leukemia, was identified as a binding-partner of U19/EAF2. FB1 also binds to EAF1, the only homologue of U19/EAF2. FB1 also interacts and co-localizes with ELL in the nucleus. Interestingly, FB1 inhibited the transcriptional activity of U19/EAF2 but not EAF1.Conclusions: FB1 is an important binding partner and a functional regulator of U19/EAF2, EAF1, and/or ELL. [ABSTRACT FROM AUTHOR]

The kinetics of free sphinganine (Sa), sphinganine to sphingosine ratio (Sa/So), proteins, cholesterol, alanine aminotransferase (ALAT) and lactate dehydrogenase (LDH) were investigated in the course of fumonisin B1 (FB1) exposure in ducks (20 growing males divided into four groups of 5 receiving, respectively, a daily dose of 0, 5, 15 or 45 mg/kg FB1 via oral administration over 12 days). Descriptive statistics of these parameters were also studied in a large number of ducks not exposed to mycotoxins and free of known pathology. Although the toxin at the end of the treatment affected all the parameters investigated, only 2 days of treatment appeared necessary to increase free Sa concentrations in serum, whereas 6 days were necessary to detect a significant effect on Sa/So ratio. Significant differences between control and treated ducks were observed after 4 days of treatment for ALAT and LDH and after 6 and 8 days for cholesterol and proteins concentrations. The minimum doses of FB1 required to determine an effect were assessed using three different methods. This approach reveals that FB1 has greater effects when it is ingested at a low dose for a long time than when ingested at a high dose for a short time. Although the minimum toxic dose of FB1 in ducks remains to be determined, this result must be considered in the context of chronic exposure to the toxin, not only in avian populations. [Copyright &y& Elsevier]

Fumonisin B1 (FB1), a mycotoxin produced primarily by Fusarium veticillioides and related fungi, is a carcinogen and causative agent of various animal diseases. Our previous studies indicated the involvement of tumor necrosis factor-α (TNFα) in FB1-induced hepatotoxicity. Male B6,129 mice (five/group) were injected subcutaneously with vehicle or 2.25 mg/kg/day of FB1 for 5 days and sampled 1 day after the last treatment. FB1 treatment caused an increased expression of TNFα, interferon γ (IFNγ) and interleukin (IL)-12 p40 in liver without any changes in kidney or spleen, suggesting the localized site of their production. IL-1β cytokine expression was increased in liver and kidney after FB1 exposure. Cells involved in TNFα production after FB1 treatment in liver were identified as Kupffer cells. FB1 increased alanine aminotransferase in plasma and increased apoptotic cells in liver. Selective increase in proinflammatory T helper (Th)1-cytokines (IL-12 and IFNγ) and TNFα with no alteration in Th2-cytokines (IL-4, IL-6 and IL-10) suggest the involvement of IL-12, produced by Kupffer cells, in induction of IFNγ production by natural killer (NK) cells and/or NK1+ T cells, which can undergo a positive amplification loop with TNFα produced by macrophages or other hepatic cells to elicit the toxic reaction. [Copyright &y& Elsevier]

Fusarium verticillioides is one of the major fumonisin producers. The ingestion of this mycotoxin represents a risk for both human and animal health. The development of F. verticillioides is associated with environmental conditions, especially carbon sources. We developed a reliable and fast reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay and determined fum1 gene expression upon growth of two F. verticillioides strains isolated from maize and wheat in Czapek's medium containing four different sugars as sole carbon sources. Fumonisin B 1 (FB 1) production was determined by LC-MS/MS analysis. High growth and production of FB 1 were observed in fructose-containing medium for the strain that originated from maize. Less production of FB 1 occurred using maltose as sole carbon source for both strains. The fum1 gene expression started between 2 and 4 days of incubation, and positive signals were detected prior to the initial production of FB 1. The RT-LAMP assay was effective in the detection of fum1 gene expression at very early stages of F. verticillioides growth and allowed the prediction of FB 1 formation. • RT-LAMP primers were designed to detect fum1 gene expression. • RT-LAMP results were detected directly after incubation by visible light. • RT-LAMP positive signals occur prior to the initial production of FB 1. • Fructose induced high production of FB 1 for F. verticillioides strain from maize. • A lower amount of FB 1 was produced with maltose as carbon source by F. verticillioides strains. [ABSTRACT FROM AUTHOR]

The aim of this study was to detect and quantify fumonisin B1(FB1) in cereal mixtures marketed in Brazil. Fifteen samples from different lots were acquired each month by internet from supermarkets during seven months, adding up to 105 analysed samples. The unit sample constituted of an original package with a minimum of 250 g. Extraction and clean-up of samples for FB1determination were carried out using immunoaffinity columns. Identification and quantification of FB1were performed by high performance liquid chromatography. Eighty-eight (83.8%) samples were contaminated with FB1and four (3.8%) presented levels above 500 µg kg−1(634, 703, 1269 and 1876 µg kg−1). Maximum FB1 + FB2levels allowed by Brazilian regulations will reach 1500 µg kg−1for corn flour in 2016 and 1000 µg kg−1for others corn products. This study showed that even at levels below the legislative limits, human exposure to this toxin can occur constantly. [ABSTRACT FROM AUTHOR]

In order to study the formation of fumonisin artefacts and the binding of fumonisins to matrix components (e.g. starch and protein) in thermal treated food, model experiments were performed by heating fumonisin FB1 with amino acid derivatives (protein model) and methyl-a-D-glucopyranoside (starch model). The reaction products were analysed by highperformance liquid chromatography-electrospray ionisationtandem mass spectrometry (HPLC-MS/MS). Using MS/MS experiments the formed reaction products were characterized as conjugates between fumonisin B1 and the used substrates. The reaction product between fumonisin B1 and methyl-α-D-glucopyranoside was purified and identified by nuclear magnetic resonance (NMR) spectroscopy as the diester of fumonisin B1 and methyl-α-D-glucopyranoside. These studies indicate that fumonisins can bind to matrix components via their TCA side chains. [ABSTRACT FROM AUTHOR]

Fumonisin B1 (FB1) is a common contaminant of corn (Zea mays L.) that causes pulmonary edema in pig with high mortality. Clay products have been used as absorbent of FB1 to reduce its effects. FB1 contamination in pig is difficult to detect and swine hair may be used as a non-invasive biomarker for the presence of this toxin. The aim of this study was to evaluate the presence of fuminisin B1 in swine hair in swine groups exposed to FB1 with clay. Male swine were fed with standard laboratory chow, tap water and housed indoors under controlled conditions for 21 days. They were divided in four groups of 8 pigs: control (Group A), 15 mg of fungal culture kg-1 (Group B), 15 mg of fungal culture kg-1 and 0.15% of experimental clay (Group C), 15 mg of fungal culture kg-1 and 0.20% of experimental clay (Group D) and evaluated in the 3rd and 6th weeks. Hair samples were stirred in ethanol for 15 minutes and dried at 50 oC for 3 hours, cut into small pieces. 0.5 g of swine hair samples were extracted with 20 mL of methanol overnight at 50 oC. The extract was filtered, and 15 mL was dried, resuspended in 20 mL of methanol: water (70:30), added 8 mL of water and filtered again. The solution was washed with 5 mL of hexane, centrifuged at 3000 rpm and top layer was discarded. pH was adjusted to 6. An aliquot of 20 mL of the extract was passed through the SAX column and eluted with 14 mL of methanol: acetic acid (0.5%). The eluted was dried, resuspended in 300 μL of water: acetonitrile (90:10), centrifuged and injected in HPLC. On the 3rd week, fumonisin B1 was detected in levels of 20.9, 11.4 and 18.5 ng.g-1 on group B, C and D, respectively. On the 6th week, toxin was identified only on group B at a level of 1.3 ng.g-1 and in the both weeks, it was not possible to identify FB1 on the control samples. Swine hair can accumulate FB1, however clay showed an interference in the toxin absorption, decreasing it. [ABSTRACT FROM AUTHOR]

Weaned rabbits were fed diets contaminated with 2 mg/kg diet T-2 toxin alone, or 10 mg/kg diet fumonisin B 1 (FB 1 ) alone, and both toxins in combination (2 + 10 mg/kg, respectively) compared to a toxin-free control diet. Samplings were performed after 4 weeks (blood and liver). Bodyweight of T-2-fed group was lower after 4 weeks; the liver weight was increased dramatically (threefold of control). Liver total phospholipids (PLs) provided slight alterations in the fatty acid (FA) composition; all three toxin-treated groups showed a decrease in palmitoleic acid (C16:1 n7) proportion. In the liver mitochondrial PL FA composition, margaric acid (C17:0) proportion decreased in the separated toxin treatments compared to the combined setting. Oleic acid (C18:1 n9) proportion was increased and arachidonic acid (C20:4 n6) was decreased in the FB 1 -treated group, while docosapentaenoic acid (C22:5 n3) was decreased in the separated treatments. The total monounsaturation was significantly higher in the FB 1 group's mitochondrial PL FA profile. After 4 weeks, all toxin treatments decreased the blood plasma reduced glutathione and glutathione peroxidase activity, and FB 1 increased the plasma sphinganine/sphingosine ratio. Both mycotoxins seem to cross the hepatocellular and the hepatic mitochondrial membrane, without drastic membrane disruption, as assessed from the PL FA composition, but inducing detectable lipid peroxidation.

In the present study, we evaluated the nephrotoxicity of individual mycotoxins and combinations of aflatoxin B1 (AFB1), zearalenone (ZEA), deoxynivalenol (DON), and fumonisin B1 (FB1) to livestock using porcine kidney 15 cells (PK-15) as a disease model via biochemical approaches. The toxicity of individual mycotoxins on cell viability and cell membrane damage was determined using the MTT and lactate dehydrogenase (LDH) assays, respectively. Individual cytotoxicity of mycotoxins in increasing order were FB1

The individual and combined effects of dietary aflatoxin B(1 )(AFB(1)) and fumonisin B(1) (FB(1)) on liver pathology, serum levels of aspartate amino-transferase (AST) and plasma total protein (TP) of broilers were evaluated from 8 to 41 days of age. Dietary treatments included a 3 × 3 factorial arrangement with three levels of AFB(1 )(0, 50 and 200 μg AFB(1)/kg), and three levels of FB(1 )(0, 50 and 200 mg FB(1)/kg). At 33 days post feeding, with the exception of birds fed 50 mg FB(1 )only, concentrations of AST were higher (p < 0.05) in all other treatment groups when compared with controls. Plasma TP was lower (p < 0.05) at six days post feeding in groups fed 200 μg AFB(1)/kg alone or in combination with FB(1). At day 33 days post feeding, with the exception of birds fed the highest combination of AFB(1 )and FB(1 )which had higher plasma TP than control birds(, )plasma TP of birds fed other dietary treatments were similar to controls. Broilers receiving the highest levels of AFB(1) and FB(1) had bile duct proliferation and trabecular disorder in liver samples. AFB(1) singly or in combination with FB at the levels studied, caused liver damage and an increase in serum levels of AST.