1. Stable shuttle vectors for Neisseria gonorrhoeae, Haemophilus spp. and other bacteria based on a single origin of replication
- Author
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P.A. Totten, Jo-Anne R. Dillon, F.J. Pagotto, and Hossein Salimnia
- Subjects
Recombinant Fusion Proteins ,Genetic Vectors ,Green Fluorescent Proteins ,DNA, Recombinant ,Haemophilus ,Cloning vector ,Replication Origin ,Biology ,urologic and male genital diseases ,Origin of replication ,medicine.disease_cause ,Microbiology ,Haemophilus influenzae ,Transformation, Genetic ,Plasmid ,Shuttle vector ,Escherichia coli ,Genetics ,medicine ,Bacteria ,Genetic Complementation Test ,General Medicine ,biology.organism_classification ,Virology ,Neisseria gonorrhoeae ,female genital diseases and pregnancy complications ,Luminescent Proteins ,Haemophilus ducreyi - Abstract
An origin of replication (ori) was obtained from a naturally occurring β-lactamase-producing plasmid isolated from Neisseria gonorrhoeae and used to construct shuttle vectors capable of replicating in N. gonorrhoeae, Haemophilus ducreyi, Haemophilus influenzae and Escherichia coli. Using the gonococcal proAB genes, we complemented proline-requiring N. gonorrhoeae F62 and E. coli HB101 in trans. The first demonstration of the expression of the green fluorescent protein (GFP) in either N. gonorrhoeae or H. ducreyi was shown using this vector, indicating that GFP may be a useful tool in the analysis of these organisms. This is the first report of a gonococcal vector based on a broad host range, genetically defined ori, and should facilitate the molecular analysis of gonococcal and Haemophilus genes.
- Published
- 2000
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