Search

Gill diseases cause serious losses in farming of Atlantic salmon and the number of agents involved increases. Salmon gill poxvirus (SGPV) and the gill disease in causes where SGPV apparently was the only disease-causing agent were initially characterized. Recently, it was further shown that SGPV can be a common denominator in widely different multifactorial gill diseases. Here, we present the challenge of diagnosing gill disease with SGPV in salmon fry of 0,3-5 grams. Apoptosis of gill lamellar epithelial cells and hemophagocytosis was also observed in fry similar to findings in smolts and grow-out fish. Using our newly developed immunohistochemistry method, we further demonstrate that some of the apoptotic epithelial cells covering the oral cavity were positive for SGPV. Thus, SGPV is not restricted to respiratory epithelium alone and may infect the fish at very early life stages. Furthermore, as the cases examined here are from Norway, Faroe Island and Scotland, we show that SGPV is more widespread than previously reported.

Introduction: We are concerned that the dental profession, worldwide, has lost its way. We are a group of senior scientists – researchers, academics and intellectuals – from various parts of the world, with over 250 years’ combined experience of working to improve the oral health of communities. The group is entirely independent of any institution, government body or corporate entity. We met for a few days in March 2017 under the hospitality of Professor Emeritus Alfonso Escobar at his home in the Andes of Colombia, known as La Cascada Mare, to share our concerns about the future of dental care and dental education (Professor Richard Watt was unable to attend the meeting in Colombia, but was able to share an outline of his paper and to provide inputs into this declaration). Each of us prepared a detailed paper of our experiences over the last half century, our assessment of the problems and suggestions for the way forward. Each paper was discussed in detail (the full texts can be accessed at https://lacascada.pressbooks.com). The following statement represents our analysis of the problem and provides some recommendations about what should be done.

Background Hereditary thrombophilia testing identifies genetic conditions that can predispose patients to an increased risk of venous thromboembolism (VTE); these conditions include Factor V Leiden, Factor II G20210A gene mutation, protein C deficiency and protein S deficiency. Testing as an inpatient can be challenging as acute thrombosis and anticoagulation can confound the study results. As a result, major society guidelines do not recommend routine hereditary thrombophilia testing in an acute setting. Objective The purpose of this study is to retrospectively review the ordering patterns and outcomes of hereditary thrombophilias in inpatients. Methods All hereditary thrombophilias ordered for inpatients between May 2016 to April 2019 at four major academic centers, Foothills Medical Centre, Peter Lougheed Centre, South Health Campus and Rockyview General Hospital in Calgary, Alberta were retrospectively reviewed. The primary outcome was the indication for thrombophilia testing and the results of the testing. Secondary outcomes included patient demographics and thrombosis risk factors. Results 428 patients were identified as having had hereditary thrombophilia testing as inpatients. 411 patients had a documented thrombotic event with 400 patients (93.4%) having an event within 30 days of their testing. 287 patients (69%) were on anticoagulation at the time. 60.1% of patients had a venous thrombosis, with pulmonary embolism being the most common (28.2%) while 39.9% of patients had an arterial thrombosis with 19% being a stroke. 177 (43%) of patients had another provoking risk factor at the time of their thrombosis, including malignancy, recent major surgery, pregnancy and exogenous estrogen use. The majority of testing was ordered by General internal medicine (30.1%) and neurology (25%). Only 3.9% of patients had hematology involvement at the time of their testing. 77 (18%) patients had a positive thrombophilia screen with heterozygous Factor V Leiden (11.4%) and heterozygous Factor II G20210A (5.1%) variant being the most common. Prior VTE was associated with an increased likelihood of a positive thrombophilia result (RR 2.15, CI 1.43-3.23, p= 0.0002) but family history, arterial thrombosis, and venous thrombosis at unusual sites were not. Conclusion The utility of hereditary thrombophilia testing is limited in an inpatient setting, particularly for patients with acute thrombosis and on anticoagulation. The majority of tests were ordered inappropriately and resulted in unnecessary costs to the healthcare system. This study indicates the need for further interventions to improve physician education and adherence to guidelines.

We investigated the impact of a short-lived policy of charging fees to patients attending public-sector outpatient health facilities in Kenya by collecting data on attendance at Nairobi's Special Treatment Clinic for sexually transmitted diseases (STDs) before (23 months), during (9 months), and after (15 months) the user-charge period. During the user-charge period, the seasonally adjusted total mean monthly attendance of men decreased significantly to 40% (95% Cl 36-45) of that before fees were levied. Attendance rose in the post-user-charge period, but reached only 64% (59-68) of the pre-user-charge level. For women, the adjusted total mean monthly attendance during the user-charge period was reduced significantly to 65% (55-77) of the pre-user-charge level. Mean monthly attendance by women rose in the post-user-charge period to 22% (9-37) above the pre-user-charge level. There was no evidence of an increase in attendance over the course of the user-charge period among either men or women. The introduction of user fees probably increased the number of untreated STDs in the population, with potentially serious long-term health implications. The user-fee experience in Kenya should be carefully evaluated before similar measures are introduced elsewhere.

Extracts from five plants used as chewing sticks, and tannic acid, gallic acid and methyl ester of gallic acid, were tested for their ability to inhibit proteolytic activities of three strains of Bacteroides gingivalis, three strains of Bacteroides intermedius and two strains of Treponema denticola. Aqueous extract from the plants Rhus natalensis and Euclea divinorum were the most inhibitory of those tested, inhibiting by 50% the proteolytic activity of the test organisms, at concentrations of up to 200 micrograms/ml. Tannic and gallic acids had similar effects at concentrations of less than 10 micrograms/ml, while the methyl ester of gallic acid was less inhibitory. These findings suggest that extracts from plants used as chewing sticks may possess enough inhibitory components to interfere with the virulence and growth of periodontopathic bacteria in vivo, provided they are able to gain access to the subgingival sites such bacteria preferentially inhabit.

Objectives. The aim of this study was to assess the usefullness of 18-fluorodeoxyglucose positron emission tomography (PET) scanning for the evaluation of metastases (nodal and distant) in patients with carcinoma of the cervix. Methods. A retrospective review was performed of 13 patients with carcinoma of the cervix who had a 18-fluorodeoxyglucose PET scan as part of their workup (10 during initial workup, 3 at time of relapse). Ten patients also underwent a fine needle aspiration (FNA) under imaging guidance for verification. Results. All 10 patients with positive sites identified by PET scan who underwent an FNA were positive for cancer. In 3 situations PET identified sites where other imaging studies were negative. Conclusions. PET scanning is a useful imaging tool in the evaluation of patients with carcinoma of the cervix. This review supports other limited published data in this regard and suggests that further prospective studies are needed.

The King Faisal Specialist Hospital and Research Center opened in 1975 to provide specialized medical treatment to the citizens of Saudi Arabia and to promote prevention of disease through research and education. It is a national and middle eastern tertiary care hospital for oncology and a principle center for radiation therapy in Saudi Arabia. In this retrospective study, 504 patients with carcinoma of the cervix were analysed. Of the 504 patients, 410 received treatment with curative intent, which comprised radical surgery alone, radical radiation alone or combination surgery and radiation. The overall 3, 5 and 10 years survival of all 504 patients was 64%, 55% and 40%, respectively and for 410 patients treated with curative intent it was 74%, 61% and 51%, respectively. Multivariate analysis, using the Cox regression method, showed that the stage of the disease is the only significant prognostic factor influencing relapse-free survival. Stage for stage, treatment outcomes at King Faisal Specialist Hospital and Research Center match those achieved in the First world, but two-thirds of patients (rather than one-third) present with advanced disease at diagnosis. Therefore, any improvement in overall outcomes will require earlier diagnosis, achievable only through an effective screening program in the Kingdom of Saudi Arabia and similarly in the other Third world countries where patients present with advanced stage disease.

Problems arise when attempting to compare caries data collected in both Africa and the People's Republic of China. These difficulties are not only the result of differences in the criteria used for the diagnosis of caries but are also due to the diversity of cultural and social conditions existing within each of the geographical areas. However, interpreted with caution, the data seem to suggest that caries experience among children is still fairly stable and at a low level, in contrast to the predictions of a decade ago. When comparing data from adult and elderly cohorts, however, caries is seen to be a widespread disease with continuing slow progression throughout life.

The hypothesis that the Stephan pH responses of dental plaque would be different in caries-active and -inactive individuals was tested in 20 seven-year-old and 19 14-year-old Kenyan children. In each age group, half the children had ≥ 2 dentin cavities; the other half had no such lesions. With a palladium-touch microelectrode, interdental plaque pH was monitored between m1/m2 in each quadrant in the primary dentition and in the four molar/premolar regions in the permanent dentition. pH was also monitored in caries cavities in the occlusal surfaces of lower first molars and on the tongue. pH was measured before and up to 60 min after the children rinsed with 10 mL of 10% sucrose. Caries status of the individual was unrelated to plaque pH in comparable non-carious sites in both of the age groups. The pH minimum in the maxilla was about 0.5 pH units lower than that in the mandible. Active occlusal caries lesions had a resting pH value of about 5.5, about 1 pH unit lower than that of sound surfaces. The pH dropped to about 4.5 in caries lesions and recovered slowly. In sound occlusal sites, a pH drop to about 6.0 was followed by a relatively rapid return to the resting value. Thus, when the mean values were considered, the classic Stephan curve response was evident. However, when the pH changes at single sites were considered at various time intervals, a substantial, erratic fluctuation was observed. The tongue had a resting pH value of 7.0, which dropped to about pH 5.5 and remained low for more than 60 min.

The aim of this study was to assess the applicability of palladium touch micro-electrodes, connected to battery-run pH meters, for in vivo plaque pH measurements in children. The pH was assessed in 20 7-year-old and in 19 14-year-old caries-active and caries-inactive rural Kenyan children. The resting pH was measured at non-carious interproximal and occlusal sites and in open dentine cavities. Independent repeated measurements were performed at given sites at intervals of 15 s and 5 min and on different days. The resting plaque pH varied widely among the children, and there was no significant difference between caries-active and caries-inactive groups. The most striking feature was the considerable erratic fluctuations of pH at a given site with time, both in resting and in sucrose-challenged plaque. These fluctuations were sensitively recorded by palladium touch microelectrodes. After a sucrose rinse, not all sites in the same mouth behaved in a similar fashion, and thus the classical ‘Stephan curve’ was not always apparent. In conclusion, the palladium touch microelectrodes are highly applicable for plaque pH measurements in children, even under extreme field conditions.

In 5 rural Kenyan children, the effect of sugarcane chewing on plaque pH was compared with the effect of a mouthrinse with 10% sucrose at various intraoral sites. They all had poor oral hygiene and at least two carious cavities in occlusal surfaces of molars. pH measurements were conducted under field conditions using paladium touch microelectrodes connected to a battery-operated pH meter. There was a marked difference in pH response of non-carious approximal sites between maxilla and mandible, with the lowest values in the maxilla. However, the pH recovery following the instantaneous drop occurred in parallel even if most pH values had not returned to baseline values 30 min after the sucrose rinse. Following the sugarcane chewing, the pH fall was less pronounced on all sites, and within 5-10 min the values had returned to resting pH and even exceeded this. In carious cavities, a similar pattern was observed, although the acidity in these sites was more pronounced, also reflected in a lower resting mean pH. The main conclusion from this study is that sugarcane chewing yields a less pronounced pH drop and a quicker pH recovery in dental plaque than is seen following a mouthrinse with 10% sucrose. This difference probably results from stimulation of salivary flow associated with the chewing.

Udgivelsesdato: 1992-Jan The aim of the study was to assess the occurrence of some putative periodonto-pathogens in "test" and "control" sites in "diseased" and "non-diseased" persons, respectively, from an adult rural Kenyan population exhibiting poor oral hygiene and widespread loss of attachment (LA). 14 persons (less than 35 years) were assigned to a "diseased" category on the basis of at least 4 sites with LA greater than or equal to 4 mm; at least 5 mm LA and a pocket greater than or equal to 4 mm interproximally in a lower incisor ("test" site): and less than 2 mm LA and no pocket greater than or equal to 4 mm distal to a lower canine or mesial to a lower first premolar ("control" site). Age-matched "non-diseased" persons were identified on the basis of no sites with LA greater than 2 mm and no pockets greater than or equal to 4 mm associated with LA. Paperpoint samples from test and control sites as well as a scraping sample from the dorsum of tongue were examined for presence of Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Bacteroides intermedius, B. melaninogenicus group, Capnocytophaga, Selenomonas spp., and Wolinella recta. P. gingivalis was found in 79% of test sites and 36% of control sites in "diseased" persons, and in 18% and 35% of test and control sites, respectively, in "non-diseased" persons. "No other bacterial group discriminated significantly between test and control sites or between diseased and non-diseased subjects. The surprisingly high occurrence of P. gingivalis in non-diseased subjects, both subgingivally and on tongue, indicates that deep periodontal pockets are not prerequisite ecological environments for P. gingivalis establishment.

This report is a 2-year follow-up of a baseline study conducted in Tanzania in 1984. 377 and 448 twelve-year old primary school children were examined in Dar es Salaam (urban) and rural areas respectively. The same criteria and examiners were employed to replicate conditions in 1984 as closely as possible. The mean DMFT score has remained stable over the 2-year period in both the urban (1984: 0.67 (s.d. 1.20); 1986: 0.64 (s.d. 1.32) and rural (1984: 0.51 (s.d. 1.07); 1986: 0.40 (s.d 0.93) areas. However, a significant decrease in the mean DMFS score in Dar es Salaam from 1.19 (s.d. 2.71) in 1984 to 0.81 (s.d. 1.96) in 1986, and in rural areas from 0.78 (s.d. 1.98) in 1984 to 0.48 (s.d. 1.44) in 1986 was observed. In 1986 significantly fewer children with caries had more than 5 involved surfaces in both urban and rural areas than in 1984. In the two areas the difference in caries prevalence observed between 1984 and 1986 affected different surfaces types to varying extents. The results do not support the finding that caries is increasing in all developing countries.

We argue that the nature of the economic crises in sub-Saharan Africa cannot be understood outside the context of the legacy of colonialism and class formation. Structural adjustment programmes serve to exacerbate inequalities and threaten to reserve the social gains of the majority achieved through the struggle for independence, in the interest of the indigenous capitalist class. Under such circumstances social scientists have a social responsibility to take a stand against the current policies that have led to an unprecedented decline of the health status of the poor; their skills must be put at the disposal of the oppressed with a view to giving voice to the experiences and needs of the majority.

Water is a major source of fluoride ions in areas where skeletal and dental fluorosis are endemic. We investigated the capacity of bone char to remove fluoride from water and its effects on selected bio-indicator organisms. Under static and dynamic conditions, the capacity of bone char was in the order of 2.5 mg of fluoride per g of bone char. Bone charcoal did not appear to support growth of yeast and S. aureus. E. coli and S. faecalis counts in the filtrate decreased with time but there was substantive growth in the bone charcoal.

317 children born and raised in two rural areas of Machakos District in Kenya were examined for enamel changes. All sources of drinking water were monitored for F–– over a period of 1 year.

The aim of this study was to describe the variations with time in the concentrations of fluoride in drinking water sources in Greenland, Kenya, Greece, Denmark and Ireland. Water samples were collected monthly and shipped to laboratories in Aarhus for electrometric analyses. In Narssaq, Greenland the fluoride concentration of a single piped water supply ranged from 0.3 to 2.8 ppm, the variations being related to climate, precipitation and temperature over the year. Water from the Athi River, Kenya had a fluoride content ranging from 0.3 to 1.2 ppm, the higher concentrations being associated with the dry seasons. The fluoride concentration in piped water from mountain rivers in Mourjes, Greece, ranged over the year between 1.3 to 2.0 ppm, the changes being apparently unrelated to rainfall. Marked variations in fluoride concentrations from 0.5 to over 3.5 ppm were observed in water from artesian wells in Assiros, Greece. In drinking waters from boreholes in Boennerup Strand, Denmark, fluoride concentrations ranged with time from 1.4 to 2.4 ppm, the variations being unrelated to climate or precipitation, while little variation in fluoride concentrations was found in water from boreholes in either Roedvig or Egens, Denmark. Water obtained from two sources of artificially fluoridated water supplies from Ireland showed considerable variations with time, although pooled samples indicated relatively constant levels over the year. The study indicates that the results of single fluoride ion measurement from any given source should not be taken as being a reliable indicator of fluoride exposure from drinking water.(ABSTRACT TRUNCATED AT 250 WORDS)

A diagnostic modality to study parasternal lymph nodes was examined at the Cancer Control Agency of British Columbia, Vancouver. Forty-five patients with carcinoma of the breast were studied. The data correlate well with that from other centres in regard to anatomic validity, lymph node distribution, cross drainage and findings in relation to the stage of disease. The frequency of lymphoscintigraphic abnormalities was almost twice as high in relation to lesions situated in the centre and inner half of the breast as compared with outer half lesions. Twenty-eight per cent of patients demonstrated cross drainage between parasternal lymphatics. In 11% of the patients, nodes were situated at locations greater than 4 cm from the midline. Work is in progress to study the correlation of lymphoscintigraphy with disease in the nodes. The data indicate that this study can be used in other centres in the management of patients with mammary carcinoma.

Children aged 11–15 years from three low-fluoride zones (< 0.5 ppm in drinking water) situated at sea level, 1,500 m and 2,400 m above sea level, and from two higher-fluoride zones (0.5–1.0 ppm in drinking water) were examined for dental fluorosis. In the low-F zones 36.4% of the children at sea level had dental fluorosis, as compared to 78.0% at 1,500 m, and 100.0% at 2,400 m. In the higher-F zones 71.2% had dental fluorosis at sea levels as compared to 93.8% at 1,500 m (p < 0.001). The intraoral pattern of dental fluorosis was similar in each of these five populations, and the severity of dental fluorosis of each tooth type increased significantly with increases in altitude in both the low and higher-fluoride zones (p < 0.001). Moreover the severity of dental fluorosis was associated with increases in fluoride concentrations at constant altitudes (at sea level and at 1,500 m). This study establishes for the first time that populations living at high altitudes may be more susceptible to dental fluorosis than those at low altitudes for a given concentration of fluoride in drinking water. The findings indicate that certain physiological changes may occur in humans living at high altitude whereby the effects of fluoride on mineralizing tissues become exacerbated.