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In this article, we report the silver orthophosphate (Ag3PO4) photocatalytic inactivation properties on strains Gram-positive Saprophyte B. subtilis, a diploid fungus Candida albicans and Gram-negative Pseudomonas aeruginosa, using as irradiation source white-light-emitting diodes (WLEDs) with luminous flux (Φ v) of 1.3 × 103 Lumens and relative power density of 15 mW m−2. Microorganisms death curves and the kinetic constants (Kd) indicated that the inhibitory effect of the Ag3PO4 under WLEDs irradiation is well pronounced to C. albicans (6.6 × 10–2 min−1) in relation to P. augenosas (4.6 × 10–2 min−1) and B. subitilis (2.5 × 10–2 min−1). Decimal reduction time (Dt) were 34.4 min to C. albicans, 50.1 min P. augenosas and 92.1 min to B.subitilis. The micrographs obtained by scanning electron microscopy with field emission gun (SEM-FEG) demonstrated that there was cell wall permeability and consequently total rupture in the C. albicans, suggesting the lipid peroxidation phenomenon and protein oxidation promoted by the presence of reactive oxygen species (ROS). Furthermore, it was observed the Ag3PO4 when irradiated by WLEDs demonstrates important sporicidal activity in related to B. subtilis, promoting the endospore wall rupture.

A verticalização de edifícios para moradia foi uma solução encontrada, pós Revolução Industrial, para suprir os problemas do aumento populacional nos grandes centros urbanos a partir de 1920 e se tornou a principal forma de residir nas cidades ao longo do tempo. O presente artigo teve como objetivo analisar as transformações das fachadas das edificações multifamiliares na cidade de Vitoria/ES ao longo desse processo de verticalização. A pesquisa analisou uma amostra de 629 empreendimentos que permitiram a elaboração de cinco modelos representativos separados com base nas alterações legislativas e de desenvolvimento urbanístico local. Constatou-se algumas alterações construtivas nas fachadas, principalmente a inserção de varandas e a substituição do vidro incolor pelos coloridos fumê/bronze e verde. Entretanto, observa-se que a maior parte das características construtivas na região de estudo permanecem as mesmas ao longo do tempo, tais como, a verticalização de baixa e média altura, a utilização de janelas de correr e a aplicação de pinturas de cores claras e pastilhas nas fachadas.

Enzimas lipoliticas ou lipases estao envolvidas na degradacao de lipidios e desenvolvem um importante papel na reciclagem de compostos insoluveis em agua. Estao amplamente distribuidas na natureza, podendo ser de origem animal, vegetal e microbiana. As principais fontes de obtencao de lipases para aplicacao industrial tem sido os microrganismos eucariotos (leveduras e fungos). Contudo, faz-se necessaria a busca por mais microrganismos produtores de lipase em diferentes condicoes de cultivo. Assim, este trabalho objetivou realizar a prospeccao tecnologica da utilizacao da lipase obtida por fermentacao de leveduras afim de se avaliar o perfil quantitativo das publicacoes de artigos e protecoes por meio de patentes relativas aos processos envolvendo esse grupo de microrganismo no indexador Web of Science e a base de dados de patentes, Derwent Innovation Index, Europen patent Office (EPO), no United States Patent and Trademark Office (USPTO), World Intellectual Property Organization (WIPO) e no banco de dados do Instituto Nacional de Propriedade Industrial (INPI) do Brasil. Para determinar a evolucao biotecnologica envolvendo a enzima lipolitica de levedura foi realizada um levantamento de patentes e de artigos cientificos publicados, em junho/2015. Encontrou-se 1.228 artigos, e cerca 918 patentes na Derwent Innovation Index, 241 na EPO, 219 na WIPO, 10 na USPTO e 1 no INPI relacionados a lipase provenientes de levedura. A prospeccao tecnologica se destaca por uma evolucao a partir da decada de 90 e pelo baixo registro de patentes no Brasil em relacao ao numero de artigos.

Os relatos da esquistossomose mansônica acometendo o sistema nervoso central (SNC) são pouco frequentes, sendo raramente descrito no encéfalo, onde os ovos do Schistosoma mansoni usualmente provocam reação granulomatosa comportando-se como lesão expansiva. Um paciente do sexo masculino, procedente de São Vicente de Ferrer, Baixada Ocidental Maranhense, área endêmica da doença, 27 anos de idade, apresentou tumor no cerebelo, o qual foi tratado cirurgicamente. O estudo histopatológico da lesão cerebelar, demonstrou extensa reação granulomatosa ao redor de ovos em desintegração de Schistosoma mansoni. Os granulomas exibiam diferentes estágios de evolução, desde necrótico exsudativo até produtivo. Este caso representa o primeiro de neuroesquistossomose relatado no estado do Maranhão. A evolução pós-operatória do paciente caracterizou-se por déficit e incoordenação motora dos membros superior e inferior direito associado a tremores.

The involvement of the central nervous system in the Schistosomiasis mansoni is uncommon and in the literature exist few reports in relation this occurrence in the brain, in that the schistosoma eggs, usually promote a granulomatous reaction that remember an expansive tumour. A patient, male sex, from São Vicente de Ferrer (MA), 27 years old, had a tumour in the cerebellum, that had surgical therapy. Later histological studies of the cerebellar material of the patient, demonstrated an extensive granulomatous reaction around schistosoma eggs. The granulomatous reaction present different stages of evolution, since degenerative necrosis to organization granulomatous reaction. This is the first report from Maranhão (Brazil). The evolution of the patient was characterized for incoordination of members and trembling.

We report a surgical technique in which donor sclera is used to create a flap in glaucoma filtering procedures. This simple technique is helpful when the sclera is too thin to safely and effectively form an adequate scleral flap. The use of donor sclera allows the procedure to continue as a guarded procedure and prevents early postoperative hypotony.

The aim of the work was to study the effects of year period, technical team, breed, beef cattle and dairy cattle on the pregnancy rates in fresh embryos used in bovine transfer of IVF programs. The study was carried out at the fertilization laboratory In Vitro Nyltta Britto de Carvalho, in partnership with In Vitro Brazil, located at the Boa Vista farm, Barra do Pirai, during August 2007 to September 2008, seeking subsidies to improve the use of the technique in the field. During that period, aspirations and inovulations in 3 different periods I (August to December), II (January to April), and III (May to September) were carried out. The jobs were accomplished by 9 technical teams (A, B, C, D, E, F, G, H, and I) rendering services to the laboratory, by working with 2 beef breeds (Brahman and Nelore) and 3 dairy breeds (Gir, Girolando, and Holstein). The different breed receivers were synchronized, and in general, from 6 to 8 days after heat, they received embryo transfer, the cervical way, under low epidural anesthesia, where each female received 1 fresh embryo of IVF. All cows were submitted to gestation diagnosis by rectal palpation and ultrasonography, in general, 42 days after embryo transfer. The numbers of embryo transferred and pregnancy rates were submitted to the chi-square test, which presented significant differences (P < 0.05). There were pregnancy rates of 36.25%a (n = 960), 39.83%a (n = 1180), and 32.59%b (n = 919) in the I, II, and III periods, respectively. Among the 9 technical teams, there were verified pregnancy rates (%) of 33.51d (n = 1313), 30.30d (n = 330), 35.00cd (n = 405), 39.24cd (n = 1060), 59.25a (n = 7), 33.33d (n = 24), 53.57bc (n = 28), 43.31c (n = 157), and 58.33ab (n = 12) for A, B, C, D, E, F, G, H, and I teams, respectively. Among breeds there were rates (%) of 36.89ab (n = 412), 34.68b (n = 1286), 35.13ab (n = 74), 38.94a (n = 1140), and 37.80ab (n = 82) for Brahman, Nelore, Gir, Girolando, and Holstein, respectively. In the study, pregnancy rates (%) of 35.21b (n = 1698) in beef cattle and 38.65a (n = 1296) in dairy cattle were observed. The differences in pregnancy rates with respect to the evaluated factors, may be explained by individual, breed, and nutritional variations of the animals. There are few data in the literature with results on the embryo transfer use of IVF bovine under field conditions.