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p53 is a signaling molecule that orchestrates smart responses to diverse stimuli and employs temporal dynamics for the selective activation of downstream target genes and ultimately for cell fate d...

The patient was a 48-year-old male with a previous history of diabetes mellitus and hypertension. The patient had persistent bleeding after vitrectomy of the left eye, and the patient was examined for abnormal coagulation factor Ⅷ combined with Ⅻ. This case suggests that we need effective control of blood glucose and blood pressure in patients undergoing vitrectomy in clinical work, and adequate examination of coagulation function. Patients with coagulation factor deficiency should be treated with plasma transfusion to supplement coagulation factors before performing the necessary surgical treatment to prevent severe bleeding.患者男，48岁，既往糖尿病、高血压病史。患者左眼玻璃体切除术后持续出血，检查患者凝血因子Ⅷ合并Ⅻ异常。该病例提示在临床工作中，需要对玻璃体切除手术患者的血糖及血压进行有效控制，详细谨慎检查凝血功能。对有凝血因子缺乏的患者应输注血浆补充凝血因子后再行必要的手术治疗，防止严重的出血情况发生。.

结直肠癌是世界上最常见的恶性肿瘤之一，其发病率和病死率在我国呈上升趋势。肠黏膜活检的病理诊断是诊断结直肠癌的主要方法。我国结直肠黏膜活检的病理诊断标准存在局限性及诸多问题，这大大影响了结直肠癌的检出及治疗。越来越多的病理医师，包括西方国家的病理医师承认结直肠黏膜内癌的存在。接受结直肠黏膜内癌的概念将使病理医师在进行黏膜活检诊断时避免诊断不足，有助于提高我国结直肠癌的检出率和降低其病死率。.

Unicompartmental knee arthroplasty(UKA) is an important surgical technique for the treatment of end-stage knee osteoarthritis, which has high requirement for the position and angle of the prosthesis. The application of three-dimensional(3D) printed in UKA reflects several characteristics such as accuracy,efficiency and safety,but the current research results show that there is still lack of consistency in the design of 3D printed guides and the standard of prosthetic position parameters. This article reviews the history, manufacturing requirements and process of 3D printed guides during UKA surgery, as well as the results of current clinical research. Whether 3D printed guides can safely and effectively improve the placement accuracy of inexperienced doctors during UKA surgery,and whether the long-term results are worthy of clinical promotion need further verification.单髁置换术（UKA）是治疗终末期膝关节骨关节炎的一种重要的外科手段，其对假体位置和角度的精准度要求较高。三维打印导板应用于UKA可以提高手术精准性，术中操作便捷，安全性高。但目前导板的设计、假体位置参数等尚缺乏统一标准。本文梳理了UKA术中三维打印导板的历史、制作要求和过程，以及目前的临床研究结果。三维打印导板是否可以安全、有效地提高无经验医师在UKA术中的假体安放精准度，以及长期结果是否值得在临床上进行推广仍有待进一步的验证。.

Most species in the genus Cypripedium (Cypripedioideae) produce trap flowers, making it a model lineage to study deceptive pollination. Floral attractants in most species studied appear to target bee species of different sizes. However, more recent publications report fly pollination in some subalpine species, suggesting novel suites of adaptive floral traits. Cypripedium lichiangense (section Trigonopedia) is an endangered subalpine species endemic to the Hengduan Mountains, China. We observed and analysed its floral traits, pollinators and breeding systems over 2 years in situ and in the lab. Cypripedium lichiangense was visited by females of Ferdinandea cuprea (Syrphidae). The pollinia were carried dorsally on the fly thoraces. The eggs of this fly were frequently found in the saccate labellum and on other floral organs, suggesting brood-site mimesis. The orchid is self-compatible, but cross-pollination produces more viable embryos. We propose a new mode of floral mimesis, humus-rich oviposition site mimicry, for C. lichiangense. Compared with the mimesis of aphid colonies attracting syrphid pollinators (subfamily Syrphinae), whose larvae are entomophagic, as reported in some Paphiopedilum species (Cypripedioideae), pollination by deceit in C. lichiangense represents a distinct and separate mode of exploitation of another saprophagic (or phytophagic) larvae syrphid lineage in the subfamily Eristalinae and appears to indicate diversity of pollination strategies in Section Trigonopedia of Cypripedium. However, this new brood-site mimesis seems to be less attractive to pollinators. As a possible adaptation to the weak attracted pollination strategy, this plant species has a long flowering period and extended lifespan of individual flowers to ensure reproductive success.

Purpose To investigate the self-management of depression among members of a Chinese community. Design and methods A cross-sectional survey was conducted in Wuhan. The Depression Prevention and Management Survey was used to identify 429 participants' stage of change, perceived benefits, process of change and self-efficacy, based on the transtheoretical model perspective. Findings A majority of participants (69.0%) were at the inactive stage of depression self-management. The mean score of the process of change was 87.62 (SD = 24.83). ANOVA analysis showed gender, education, and family function were significant influencing factors in the process of change. Practice implications Mental health nurses need to target their approach to the level of the individual based on the transtheoretical model to assist them to enhance their awareness and motivation. More consideration should be given to gender, education, and family function in the context of depression self-management.

The biosynthesis and transport of nicotine has been shown to be coordinately upregulated by jasmonate (JA). MYC2, a member of basic helix-loop-helix (bHLH) transcription factor family, is well-documented as the core player in the JA signalling pathway to regulate diverse plant development processes. Four MYC2 genes were found in the tobacco genome, NtMYC2a/2b and 1a/1b. In this study, we tested whether one of them, NtMYC2a, acts as a 'master switch' in the regulation of nicotine biosynthesis and transport in tobacco. We generated NtMYC2a knockout tobacco plants using the CRISPR-Cas9 technique and analysed the effect of NtMYC2a knockout on expression of the nicotine biosynthesis genes (NtAO, NtQS, NtPMT1a, NtQPT2, NtODC2, NtMPO1, NtA622 and NtBBLa) and transport genes (NtMATE2 and NtJAT1), as well as leaf accumulation of nicotine in the NtMYC2a knockout plants. We found that all the nicotine biosynthesis and transport genes tested in this study were significantly downregulated (>50% reduction compared with wild-type control) in the NtMYC2a knockout plants. Moreover, the leaf nicotine content in knockout plants was dramatically reduced by ca 80% compared with the wild-type control. These results clearly show that NtMYC2a acts as a 'master switch' to coordinate JA-induced nicotine accumulation in tobacco and suggests that NtMYC2a might play an important role in tobacco nicotine-mediated defence against herbivory.

Porcine circovirus-like virus P1 is a novel unclassified circovirus that was first detected in China and may be associated with post-weaning multisystemic wasting syndrome (PMWS) and congenital tremor. In this study, we detected P1 infection in pigs in Hebei Province, China, in 2017. One hundred and forty of 500 (28.0%) serum samples from 25 pig farms with different PMWS status in seven cities were P1 positive on PCR. Twelve P1 strains were sequenced, and the complete genomes of 11 P1 strains were 648 nucleotides (nt) in length, whereas that of strain ZJK02 was 647 nt, with a G deletion at position of 183 in its genome. The complete genomic and capsid protein sequences of the 12 P1 strains analysed in this study shared 98.8%-100.0% and 86.5%-100.0% identity, respectively. A phylogenetic analysis based on the complete genomic and capsid sequences of 26 P1 strains showed that the 12 P1 sequences from Hebei Province clustered on two small branches. Further studies of the evolution and pathogenesis of P1 are required.

Growth factor receptor tyrosine kinase (RTK) pathway activation is a key mechanism for mediating cancer growth, survival, and treatment resistance. Cognate ligands play crucial roles in autocrine or paracrine stimulation of these RTK pathways. Here, we show SEMA3C drives activation of multiple RTKs including EGFR, ErbB2, and MET in a cognate ligand‐independent manner via Plexin B1. SEMA3C expression levels increase in castration‐resistant prostate cancer (CRPC), where it functions to promote cancer cell growth and resistance to androgen receptor pathway inhibition. SEMA3C inhibition delays CRPC and enzalutamide‐resistant progression. Plexin B1 sema domain‐containing:Fc fusion proteins suppress RTK signaling and cell growth and inhibit CRPC progression of LNCaP xenografts post‐castration in vivo. SEMA3C inhibition represents a novel therapeutic strategy for treatment of advanced prostate cancer.

Outbreaks of trembling and shaking were reported among pigs at two pig farms in Jiangsu Province, China. Serum and tissue samples tested positive for porcine circovirus-like virus P1 and negative for classical swine fever virus, porcine circovirus type 2, astrovirus and porcine pestivirus using PCR/RT-PCR and immunohistochemical techniques. High P1 viral genome loads were identified in sera, brain and lymph node tissue samples by qPCR. In addition, one of the most notable pathological changes was dissolution of the nucleus in Purkinje cells. The results of this study provide molecular evidence of an association between congenital tremor in pigs and P1 virus.

The aim of this study was to investigate the role of the Wnt pathway in regulating the IL-34 level of lupus nephritis (LN) patients, and to explore the underlying mechanism.Human mesangial cells (HMCs) of LN patients were selected. The expression level of IL-34 was detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) and Western blot, respectively. Subsequently, HMCs were treated with the Wnt pathway antagonist, DDK1. Meanwhile, the IL-34 level in DDK1 transfected HMCs was then detected. In addition, the viability of HMCs treated with DDK1 was detected by cell counting kit-8 (CCK-8) and colony formation assay, respectively.Both the mRNA and protein levels of IL-34 were significantly upregulated in HMCs of LN patients. Higher expression of β-catenin was observed in HMCs of LN patients than those of controls, which was reduced after DDK1 treatment. Meanwhile, IL-34 level in HMCs of LN patients was significantly downregulated after DDK1 treatment. In addition, DDK1 treatment remarkably increased the proliferative ability and colony formation ability of HMCs in LN patients.IL-34 is highly expressed in HMCs of LN patients and is negatively regulated by the Wnt pathway. Furthermore, HMCs viability is remarkably enhanced after blocking the Wnt pathway.

The aim of this study was to investigate the expression levels of toll-like receptor 9 (TLR9) and interleukin-23 (IL-23) in renal tissue and serum of patients with lupus nephritis (LN), and to explore their clinical correlation.LN patients and healthy controls were enrolled in the experimental group and the control group, respectively. Blood samples, serum, and peripheral blood mononuclear cells (PBMCs) were collected. Renal lesion tissues and adjacent normal tissues of LN patients were harvested from a renal tissue biopsy. Serum level of IL-23 was detected by enzyme-linked immunosorbent assay (ELISA). Expression of IL-23 in PBMCs was determined by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) and Western blot, respectively. Meanwhile, TLR9 expression in renal tissues was accessed by immunohistochemistry staining. Subsequently, 24-h protein urine, renal tubular pathological activity index, erythrocyte sedimentation rate (ESR), serum complement C3 level, and blood albumin level of LN patients were recorded. Also, the correlation between TLR9 expression and these pathological indexes was measured by correlation analysis.Serum level of IL-23 in LN patients was significantly higher than that of healthy controls. Similarly, the mRNA and protein expressions of IL-23 in PBMCs of LN patients were markedly higher than those of healthy controls. IL-23 expression was positively correlated with renal tubular pathological activity index of LN patients. Meanwhile, TLR9 was highly expressed in renal tissues of LN patients. Furthermore, TLR9 expression was positively correlated with 24-h protein urine, renal tubular pathological activity index and ESR, whereas negatively correlated with serum complement C3 level and blood albumin level of LN patients.IL-23 is highly expressed in the serum of LN patients, and its expression is closely related to the occurrence of LN. Also, the expression of TLR9 is up-regulated in the tubulointerstitium of LN patients, which is correlated with relevant clinical indexes.

The semaphorins represent a large family of signaling molecules with crucial roles in neuronal and cardiac development. While normal semaphorin function pertains largely to development, their involvement in malignancy is becoming increasingly evident. One member, Semaphorin 3C (SEMA3C), has been shown to drive a number of oncogenic programs, correlate inversely with cancer prognosis, and promote the progression of multiple different cancer types. This report surveys the body of knowledge surrounding SEMA3C as a therapeutic target in cancer. In particular, we summarize SEMA3C’s role as an autocrine andromedin in prostate cancer growth and survival and provide an overview of other cancer types that SEMA3C has been implicated in including pancreas, brain, breast, and stomach. We also propose molecular strategies that could potentially be deployed against SEMA3C as anticancer agents such as biologics, small molecules, monoclonal antibodies and antisense oligonucleotides. Finally, we discuss important considerations for the inhibition of SEMA3C as a cancer therapeutic agent.

Collective rotational bands were observed for the first time in neutron-rich odd–odd [Formula: see text]La by means of [Formula: see text]-[Formula: see text]-[Formula: see text] and [Formula: see text]-[Formula: see text]-[Formula: see text]-[Formula: see text] coincidence measurements of prompt fission [Formula: see text] rays from [Formula: see text]Cf using multi-detector array Gammasphere. Similarity between the yrast band of [Formula: see text]La and those of neighboring [Formula: see text]La, [Formula: see text]Ce was found and interpreted as quasiparticle excitations. PES calculations suggested triaxial deformation for the ground state of 142La. Considerable triaxial and near zero octupole deformations were deduced for the yrast band of [Formula: see text]La by the TRS model calculations. The band-crossing observed in the yrast band of [Formula: see text]La was interpreted to be caused by alignment of the (h[Formula: see text])2 proton pair, and the crossing frequency was best reproduced by the TRS calculations taking into account triaxial deformations. In contrast to the interpretations for the band crossing of [Formula: see text]La, alignments of the (i[Formula: see text])2 neutron pair were found to be responsible for the band-crossing of the yrast band in the neighboring even-N [Formula: see text]La, and triaxial degree of freedom was found to play a more significant role than octupole deformations in the nucleus, although its [Formula: see text] values deduced are large.

To evaluate the effectiveness of the multidisciplinary Risk Assessment and Management Program for Patients with Diabetes Mellitus (RAMP-DM) in reducing the risks of microvascular complications.This prospective cohort study was conducted with 29,670 propensity-score-matched RAMP-DM participants and diabetes patients under the usual primary care (14,835 in each group). Study endpoints were the first occurrence of any diabetic microvascular complications, non-proliferative diabetic retinopathy/preproliferative diabetic retinopathy (NPDR/prePDR), sight-threatening diabetic retinopathy (STDR) or blindness, nephropathy, end-stage renal disease (ESRD), neuropathy and lower-limb ulcers or amputation. Log-rank tests and multivariable Cox proportional-hazards regressions were employed to estimate between-group differences in incidences of study endpoints.After a median follow-up of 36 months with41,000 person-years in each group, RAMP-DM participants had a lower incidence of microvascular complications (760 vs 935; adjusted hazard ratio [HR]: 0.73; 95% confidence interval [CI]: 0.66-0.81; P0.001) and lower incidences of all specific microvascular complications except neuropathy (adjusted HR: 0.94; 95% CI: 0.61-1.45; P=0.778). Adjusted HRs for the RAMP-DM vs control group for ESRD, STDR or blindness, and lower-limb ulcers or amputation were 0.40 (95% CI: 0.24-0.69; P0.001), 0.55 (95% CI: 0.39-0.78; P=0.001) and 0.49 (95% CI: 0.30-0.80; P=0.005), respectively.The RAMP-DM intervention was associated with lower incidences of all microvascular complications except neuropathy over a 3-year follow-up. These encouraging results constitute evidence that structured risk assessment and risk-stratified management provided by a multidisciplinary team is effective for reducing microvascular complications in diabetes patients.NCT02034695, www.ClinicalTrials.gov.

Traditional breeding objectives focusing on flower type and colour have resulted in the lack of floral fragrances in modern daylilies. Headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry (HS-SPME-GC-MS) was used to determine the contributions of floral volatiles to floral fragrances in 46 daylilies (seven species, one variety, and 38 cultivars). Thirty-seven floral volatiles were identified, including 30 terpenoids, five benzenoids, and two nitrogenous compounds. Sensory evaluation and HS-SPME-GC-MS revealed that variations in the relative emission rates and proportions of (E)-β-ocimene and linalool were mainly responsible for different fragrance patterns in Hemerocallis. The studied daylilies were grouped to six fragrance patterns: (1) intense herbaceous and pungent scent, having the highest (E)-β-ocimene emission level; (2) intense flowery and pungent scent, having medium (E)-β-ocimene and low linalool emission levels; (3) medium flowery and sweet scent, havin...

In this study, cordierite ceramic samples with very low thermal expansion are prepared by the pressureless sintering technique. The effects of sintering temperature and holding time on their sintering density and elastic modulus are investigated, while their optimal values are assessed as 1420°C and 1 h, respectively. These parameters ensure the elastic modulus of 121GPa, the relative sintering density corresponding to 95% of the theoretical value, and the coefficient of thermal expansion of |4.4|ϗ 10−7 °C.

We present a generator-coordinate method for realistic shell-model Hamiltonians that closely approximates the full shell model calculations of the matrix elements for the neutrinoless double-$\ensuremath{\beta}$ decay of $^{124}\mathrm{Sn}$, $^{130}\mathrm{Te}$, and $^{136}\mathrm{Xe}$. We treat not only quadrupole deformations but also the proton-neutron pairing amplitudes as generator coordinates. We validate this method by calculating and comparing spectroscopic quantities with the exact shell model results and experimental data. Our Hamiltonian-based generator-coordinate method produces $0\ensuremath{\nu}\ensuremath{\beta}\ensuremath{\beta}$ matrix elements much closer to the shell model ones, compared to the existing energy-density-functional-based generator-coordinate approaches. The remaining overestimation of $0\ensuremath{\nu}\ensuremath{\beta}\ensuremath{\beta}$ nuclear matrix element suggests that additional correlations may be needed to be taken into account for $^{124}\mathrm{Sn}$, $^{130}\mathrm{Te}$, and $^{136}\mathrm{Xe}$ when calculating with the Hamiltonian-based generator-coordinate method. The validation of this method may open the possibility of calculating $0\ensuremath{\nu}\ensuremath{\beta}\ensuremath{\beta}$ matrix element of $^{150}\mathrm{Nd}$ in a large shell-model space.

Despite the amenability of early-stage prostate cancer to surgery and radiation therapy, locally advanced and metastatic prostate cancer is clinically problematic. Chemical castration is often used as a first-line therapy for advanced disease, but progression to the castration-resistant prostate cancer phase occurs with dependable frequency, largely through mutations to the androgen receptor (AR), aberrant AR signaling, and AR-independent mechanisms, among other causes. Semaphorin 3C (SEMA3C) is a secreted signaling protein that is essential for cardiac and neuronal development and has been shown to be regulated by the AR, to drive epithelial-to-mesenchymal transition and stem features in prostate cells, to activate receptor tyrosine kinases, and to promote cancer progression. Given that SEMA3C is linked to several key aspects of prostate cancer progression, we set out to explore SEMA3C inhibition by small molecules as a prospective cancer therapy. A homology-based SEMA3C protein structure was created, and its interaction with the neuropilin (NRP)-1 receptor was modeled to guide the development of the corresponding disrupting compounds. Experimental screening of 146 in silico‒identified molecules from the National Cancer Institute library led to the discovery of four promising candidates that effectively bind to SEMA3C, inhibit its association with NRP1, and attenuate prostate cancer growth. These findings provide proof of concept for the feasibility of inhibiting SEMA3C with small molecules as a therapeutic approach for prostate cancer.

The oligosaccharide 3-deoxy-d-manno-oct-2-ulosonic acid (Kdo) is a key component of lipopolysaccharide in Gram-negative bacteria, and is also part of the pectic polysaccharide rhamnogalacturonan (RG-II) of the plant cell wall. The enzyme KDO-8-phosphate synthase (KDO8Ps), encoded by the 2-dehydro-3-deoxyphosphooctonate aldolase (KdsA) gene, catalyzes the first step in the synthesis of Kdo. In this study, the complete coding sequence of the KdsA gene from mulberry leaves was cloned and the primary structure of KDO8Ps was deduced. Alignment of the amino acid sequence of KDO8Ps from mulberry with those of five other plant species revealed a high level of evolutionary conservation. A phylogenetic tree analysis demonstrated a short genetic distance among KDO8Ps proteins of different species. Expression of the KdsA gene was higher in the second leaves than in the eighth leaves of mulberry, and was down-regulated under conditions of high salt or drought stress. Our results suggest that KdsA expression is important for the growth of new plant tissues, and is sensitive to harsh environments.

Prostate cancer (PCa) is among the most commonly-occurring cancers worldwide and a leader in cancer-related deaths. Local non-invasive PCa is highly treatable but limited treatment options exist for those with locally-advanced and metastatic forms of the disease underscoring the need to identify mechanisms mediating PCa progression. The semaphorins are a large grouping of membrane-associated or secreted signalling proteins whose normal roles reside in embryogenesis and neuronal development. In this context, semaphorins help establish chemotactic gradients and direct cell movement. Various semaphorin family members have been found to be up- and down-regulated in a number of cancers. One family member, Semaphorin 3 C (SEMA3C), has been implicated in prostate, breast, ovarian, gastric, lung, and pancreatic cancer as well as glioblastoma. Given SEMA3C’s roles in development and its augmented expression in PCa, we hypothesized that SEMA3C promotes epithelial-to-mesenchymal transition (EMT) and stem-like phenotypes in prostate cells. In the present study we show that ectopic expression of SEMA3C in RWPE-1 promotes the upregulation of EMT and stem markers, heightened sphere-formation, and cell plasticity. In addition, we show that SEMA3C promotes migration and invasion in vitro and cell dissemination in vivo.

Strain (intrinsic and thermo) and dead layer are inevitable at the substrate–ferroelectric film interface. They significantly affects the properties of nanoscale devices. The effect of strain and dead layer on the nonlinear electric-mechanical behavior of ferroelectric thin films is investigated based on micromechanical domain-switching model. Strain enhances the Gibbs free energy of a domain, making the domains more unstable and domain switching more continuous. Due to the existence of the dead layer between the ferroelectric thin film and substrate, the dielectric constant values and the effective electric field decrease through the film, thus leading to the decrease of the remnant polarization and increase of the coercive field. Our model, in which strain and dead layer are taken into consideration, shows improved displacement and strain versus electric field loops that agree reasonably well with the experimental data. In addition, both the electric displacement hysteresis and strain butterfly loops under various stress load have also been investigated by our model.

A compact set of equations based on the multiple subbands quasi-ballistic transport theory is developed, and is used to investigate the channel electron effective mobility in recently reported In0.53Ga0.47As/Al2O3 tri-gate n-FinFET. The extracted electron effective mobility μn is around 370 cm2/V·s at low Vg - Vth bias at room temperature and decreases with increasing Vg, and increases with increasing temperature (240-332K). It is very different from the case of Si n-MOSFETs, where the electron mobility decreases with increasing temperature. The low channel effective mobility and the ab-normal temperature dependence of μn are ascribed to the high acceptor interface trap and border trap energy densities in the conduction band energy of InGaAs. The ballistic channel resistance RBall at low Vds is calculated and compared with the measured channel resistance RCH. The low transmission coefficient T = RBall/RCH ≈ 0.06 to 0.05 indicates that there is a large room to improve the InGaAs/Al2O3 n-FinFET performance.

To construct a RNA interference lentiviral vector aimed at human ARK5 (AMPK-related protein kinase 5) gene and explore its effect on the biologic behavior of human gastric cancer SGC7901 cells.Targeting human ARK5 mRNA coding sequence, we designed three specific short hairpin RNAs (shRNAs) and constructed the lentiviral vector, then infected human gastric cancer SGC7901 cells with this vector. Afterwards, we used qPCR and Western blot for detecting the silencing effect on ARK5 gene, MTT colorimetric assay to measure the cell proliferation, cell scratch test for cell migration and Transwell for cell invasion, and flow cytometry analysis for apoptosis in cells treated with glucose starvation and TNF-α.Sequencing proved that the recombinant lentiviral vector containing ARK5-shRNA-3 was constructed successfully. Real time fluorescent quantitative PCR assay showed that the expression abundance of ARK5 gene in the normal control group, negative control group and ARK5-shRNA-3 infected group were 1.002+ 0.082, 1.001+ 0.050 and 0.140+ 0.003, respectively, showing a statistically significant difference (P0.01). Cell scratch test showed that the cell migration rate of ARK5-shRNA-3 infected group was (38.5+ 4.3)%, significantly lower than that of the normal control group [(72.4+ 6.4)%] and negative control group [(75.1+ 7.1)%, P0.01]. The results of Transwell test showed that the number of penetrating cells in the normal control group, negative control group and ARK5-shRNA-3 transfection group were 257.4±12.3, 245.7±11.6, 112.5±7.8, with a significant difference (P0.01). After glucose starvation and TNF-α-treatment for 24 h, the cell death rate of the normal control group, negative control group and ARK5-shRNA-3 group were (11.7±3.2)%, (12.3±2.6)% and (30.8±4.3)%, respectively, showing that the cell apoptosis rate of ARK5-shRNA-3 transfected group was significantly higher than that of the normal control and negative control groups (P0.01).We have successfully constructed a recombinant lentiviral vector which can efficiently silence ARK5 gene. Using it we can inhibit the proliferation, migration, invasion of tumor cells, and promote cell apoptosis under the condition of TNF-α treatment and glucose starvation.

The reliability performance of InxGa1-xAs n-type metal-oxide-semiconductor field-effect transistors with Al2O3 gate dielectric under positive-bias temperature instability stress is investigated systematically. A model of stress-induced border traps was proposed to interpret all charge pumping and I-V experimental results excellently. The stress-induced border traps include recoverable donor traps and permanent acceptor traps with respective energy densities ΔDSOXDonor(E) and ΔDSOXAcceptor(E) . The shapes of ΔDSOXDonor(E) and ΔDSOXAcceptor(E) have been extracted from experimental data. ΔDSOXAcceptor(E) mainly distributes in the conduction band of InGaAs with a tail extending to the mid-gap, whereas ΔDSOXDonor(E) has a large distribution inside the energy gap and extends to the conduction band. The high density of ΔDSOXDonor(E) in the energy gap induces large degradation in the off-current, which is particularly serious when the In composition x is raised to 0.65.

There is an increasing interest in the preparation of magnetic composite coatings for application in microelectromechanical systems (MEMS) actuators. In this paper, the CoNiMnP–BaFe12O19 nanocomposite coatings have been electrodeposited from an aqueous bath containing BaFe12O19 nanoparticles. The influence of BaFe12O19 nanoparticles concentration in the bath on the incorporated nanoparticles fraction, element content fraction, surface morphology, preferred orientation, coercivity, retentivity, and the maximum energy density of the nanocomposite coatings has been investigated. The results indicate that the incorporation of BaFe12O19 nanoparticles favours the electrodeposition of Co and Mn while depressing the deposition of Ni and P. The maximum embedded BaFe12O19 nanoparticles fraction of ∼21·8 wt-% is achieved. The incorporation of BaFe12O19 nanoparticles may refine the grain size and change the preferred orientation within the nanocomposite coatings structure. CoNiMnP–BaFe12O19 nanocomposite coatings wit...

Three Mg–Al–Zn alloys (AZ31, AZ61 and AZ91) were subjected to plane-strain compression at different temperatures (200 and 400 °C) and strain rates (10−2 and 10−4 s−1). The effect of second-phase precipitates on the texture evolution was investigated. It was found that a high level of Mg17Al12 precipitates impeded extension twinning and enhanced dynamic recrystallization, resulting in weak deformation textures.

One of the major problems of p-i-n tunneling field-effect transistor (TFET) is the reliability due to the strong electric field near the tunneling junction. In this paper, using technology computer-aided design simulation, we show that the insertion of a thin n-layer into the tunneling junction of the p-i-n TFET (p-n-i-n TFET) not only enhances its drive current, as has been previously reported, but also improves its reliability. As compared with the conventional p-i-n TFET, we demonstrate the following properties of the p-n-i-n TFET: 1) The normal component of the electric field near the tunneling junction is reduced, and therefore, the field-driven interface-trap generation can be reduced. This can be further reduced by properly aligning the gate electrode with respect to the tunneling junction. 2) The threshold-voltage shift due to the dielectric charge generated near the tunneling junction is significantly reduced. 3) The variation of the threshold voltage to the oxide and bulk thickness variations is also reduced.

Sanitary sewage, oily wastewater, toxicant containing wastewater, and domestic solid wastes are major pollutants from ships threating inland water bodies. These pollutants are in significant volumes and wide ranges. Therefore, great efforts have been invested in inland ship water pollution control policy and regulations. A local ship pollution control decision evolution Game model based on bounded rationality is established to obtain the key factors affecting decision-making by ships in water pollution control include cost for pollution control, subsidies and credits, administrative supervision and punishment, while key factors affecting decision-making by authorities include cost for administrative supervision and pressures from supervisors caused by not taking supervisions, thus providing recommendations on policy and measures for inland ship pollution. This study provides references for establishment of inland ship pollution control system.