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Objective Postdural puncture headache (PDPH) is an uncommon sequel of lumbar puncture in children. When conservative treatment with bed rest, hydration, and caffeine are ineffective, epidural blood patches are recommended and are generally effective. The purpose of this report was to highlight that when lumbar epidural blood patches fail to eliminate PDPH, diagnostic evaluation should be performed and alternative treatment sought. Case Report An unusual case is described of an 11-year-old boy with PDPH, which was successfully managed with a ventral (anterior) epidural blood patch and epidural saline infusion after headache and other symptoms failed to resolve after conservative treatment and conventionally performed blood patches. Conclusions Ineffectiveness of conservative measures and epidural blood patches performed posteriorly to resolve PDPH should lead the physician both to question the diagnosis of PDPH by pursuing radiographic confirmation of a cerebral spinal fluid leak and, furthermore, identification of its location to best direct further therapy.

Aim It is difficult to mitigate threats to marine vertebrates until their habitat use is understood. We report on a decade of satellite tracking loggerhead turtles (Caretta caretta) from an important nesting site to determine priority habitats for their protection in a region where they are known to be heavily impacted by fisheries. Location Cyprus, Eastern Mediterranean. Method We tracked 27 adult female loggerheads between 2001 and 2012 from North Cyprus nesting beaches. To eliminate potential biases, we included females nesting on all coasts of our study area, at different periods of the nesting season and from a range of size classes. Results Foraging sites were distributed over the continental shelf of Cyprus, the Levant and North Africa, up to a maximum distance of 2100 km from nesting sites. Foraging sites were clustered in (1) near-shore waters of Cyprus and Syria, (2) offshore waters of Egypt and (3) offshore and near-shore regions of Libya and Tunisia. The North Cyprus and west Egypt/east Libyan coasts are important areas for loggerhead turtles during migration. Movement patterns within foraging sites strongly suggest benthic feeding in discrete areas. Early nesters visited other rookeries in Turkey, Syria and Israel where they likely laid further clutches. Tracking suggests minimum annual mortality of 11%, comparable to other fishery-impacted loggerhead populations. Main conclusions This work further highlights the importance of neritic habitats of Libya and Tunisia as areas likely used by loggerhead turtles from many of the Mediterranean rookeries and where the threat of fisheries bycatch is high. Our tracking data also suggest that anthropogenic mortalities may have occurred in North Cyprus, Syria and Egypt; all within near-shore marine areas where small-scale fisheries operate. Protection of this species across many geopolitical units is a major challenge and documenting their distribution is an important first step.

Aim Levels of sea turtle bycatch in the Mediterranean are thought to be unsustainable. We provide a comprehensive overview of adult green turtle (Chelonia mydas) distribution during nesting, migration and foraging phases, highlighting transitory as well as residential areas of high use to facilitate adequate protection for this long‐lived, migratory species. Location Mediterranean Sea. Methods Thirty‐four females were satellite tracked from breeding grounds in the four countries with major nesting (Cyprus, Turkey, Israel and Syria) for a total of 8521 (mean: 251) tracking days in a collaborative effort to summarize the most comprehensive set of distribution data thus far assembled for this species in the Mediterranean. Results Ten foraging grounds are identified, with two major hotspots in Libya accounting for >50% of turtles tracked to conclusive endpoints. The coastlines of Egypt and Libya contain high densities of migrating turtles following the nesting season, particularly July–September, and likely also pre‐nesting (April–June). A high‐use seasonal pelagic corridor running south‐west from Turkey and Cyprus to Egypt is also evident, used by >50% of all tracked turtles. Main conclusions Bycatch levels and mortality rates for the key foraging areas and high‐density seasonal pathways identified here are largely unknown and should be investigated as a priority. We recommend that the Gulf of Sirte in Libya be explored as a potential biodiversity hotspot and considered for proposal as a marine protected area (MPA). Green turtle fidelity to nesting beaches, foraging areas and migratory pathways renders them vulnerable to localized threats but enables targeted mitigation measures and protection.

The Melbourne Congress of 2011 authorized a Special Committee on Registration of Algal and Plant Names (including fossils), which was established the following year (Wilson in Taxon 61: 878–879. 2012). Its explicit mandate was “to consider what would be involved in registering algal and plant names (including fossils), using a procedure analogous to that for fungal names agreed upon in Melbourne and included in the Code as Art. 42”, but expectations at the Nomenclature Section in Melbourne went farther than that. There was the hope that registration systems for at least some of the main groups would soon be set up, to be used and tested on a voluntary basis and, if found to be generally accepted, would persuade the subsequent Congress in Shenzhen, in 2017, to declare registration of new names an additional requirement for valid publication. The Melbourne Congress also approved mandatory registration of nomenclatural novelties in fungi, starting on 1 Jan 2013. The new Art. 42 of the Code (McNeill & al. in Regnum Veg. 154. 2012) requires authors to register any fungal nomenclatural novelty, prior to publication, with a recognized repository, whereupon they are provided with a unique identifier for each name, to be included in the protologue along with other Code-mandated information. Years before registration became mandatory, mycologists had been encouraged, often prompted by journal editors, to register their nomenclatural novelties prior to publication. Most complied. Consequently, when mandatory registration was proposed, it had strong support from the mycological community. There are currently three recognized repositories for fungal names. They vary somewhat in how they operate, but they share records of their registered novelties as soon as publication has been effected. One consequence of implementing mandatory registration is that locating new fungal names and combinations and associated protologue information is much simpler now than it was before. This makes it easier to incorporate the information into taxonomic studies and to update taxonomic treatments, inventories, and indices. A corollary is that, no matter what publication outlet an author chooses, the name cannot fail to be noticed. The positive experience in mycology makes extension of the registration concept to plants and algae a compelling idea. That experience shows that the best way to make mandatory registration of nomenclatural novelties palatable to botanists and phycologists is the establishment of trial registration at repositories with a history of involvement in and commitment to the indexing of names. Trial registration enables users to acquaint themselves with registration procedures, make suggestions on how they might be improved, and appreciate, by personal experience, the benefits of registration. Unfortunately, the task of establishing such repositories proved to be more complex and time-consuming than had been foreseen. Substantial progress has been made in the establishment of such centres (Barkworth & al., in this issue, pp. 670–672) but the Committee is not in a position to make firm proposals to regulate registration procedures, even less to make registration mandatory from a concrete future date. Nevertheless, the Committee sees it as imperative that the Shenzhen Congress be offered the opportunity to move forward with registration without having to wait six more years. In this spirit, we offer the proposals below. Proposal (276) would declare registration an ongoing concern of the botanical, mycological, and phycological community and provide the basic structure for making it possible. Proposal (277) and Prop. (278) would, in addition, define a flexible framework within which a system of voluntary registration could be developed for various categories of organisms. Proposal (279) would provide for future mandatory registration in a way that does not depend on the six-year intervals between International Botanical Congresses. Presentation of each proposal is followed by a summary of the support received from members of the Committee. Fil: Barkworth, Mary E.. State University of Utah; Estados Unidos Fil: Watson, Mark. Royal Botanic Gardens; Reino Unido Fil: Barrie, Fred R.. Missouri Botanical Garden; Estados Unidos. Field Museum Of Natural History; Estados Unidos Fil: Belyaeva, Irina V.. Royal Botanic Gardens; Reino Unido Fil: Chung, Richard C. K.. Forest Research Institute ; Malasia Fil: Dasková, Jirina. Národní Muzeum; República Checa Fil: Davidse, Gerrit. Missouri Botanical Garden; Estados Unidos Fil: Dönmez, Ali A.. Hacettepe Üniversitesi; Turquía Fil: Doweld, Alexander B.. National Institute Of Carpology; Rusia Fil: Dressler, Stefan. Senckenberg Forschungsinstitut Und Naturmuseum; Alemania Fil: Flann, Christina. Naturalis Biodiversity Center; Países Bajos Fil: Gandhi, Kanchi. Harvard University; Estados Unidos Fil: Geltman, Dmitry. Russian Academy of Science; Rusia Fil: Glen, Hugh F.. Forest Hills; Sudáfrica Fil: Greuter, Werner. Freie Universität Berlin; Alemania Fil: Head, Martin J.. Brock University; Canadá Fil: Jahn, Regine. Freie Universität Berlin; Alemania Fil: Janarthanam, Malapati K.. Goa University; India Fil: Katinas, Liliana. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División de Plantas Vasculares; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina Fil: Kirk, Paul M.. Royal Botanic Gardens; Reino Unido Fil: Klazenga, Niels. Royal Botanic Gardens Victoria; Australia Fil: Kusber, Wolf-Henning. Freie Universität Berlin; Alemania Fil: Kvacek, Jirí. Národní Muzeum; República Checa Fil: Malécot, Valéry. Universite D'angers; Francia Fil: Mann, David G.. Royal Botanic Gardens; Reino Unido Fil: Marhold, Karol. Charles University; República Checa Fil: Nagamasu, Hidetoshi. Kyoto University; Japón Fil: Nicolson, Nicky. Royal Botanic Gardens; Reino Unido Fil: Paton, Alan. Royal Botanic Gardens; Reino Unido Fil: Patterson, David J.. The University Of Sydney; Australia Fil: Price, Michelle J.. Conservatoire et Jardin botaniques de la Ville de Genève; Suiza Fil: van Reine, Willem F Prud' Homme. Naturalis Biodiversity Center; Países Bajos Fil: Schneider, Craig W.. Trinity College Hartford; Estados Unidos Fil: Sennikov, Alexander. Russian Academy Of Sciences; Rusia Fil: Smith, Gideon F.. Nelson Mandela Metropolitan University; Sudáfrica. Universidad de Coimbra; Portugal Fil: Stevens, Peter F.. Missouri Botanical Garden; Estados Unidos. University of Missouri; Estados Unidos Fil: Yang, Zhu-Liang. Kunming Institute Of Botany Chinese Academy Of Sciences; China Fil: Zhang, Xian-Chun. Chinese Academy of Sciences; República de China Fil: Zuccarello, Giuseppe C.. Victoria University Of Wellington; Nueva Zelanda

The Special Committee on Registration of Algal and Plant Names (including fossils) was established at the XVIII International Botanical Congress (IBC) in Melbourne in 2011, its mandate being to consider what would be involved in registering algal and plant names (including fossils), using a procedure analogous to that for fungal names agreed upon in Melbourne and included as Art. 42 in the International Code of Nomenclature for algae, fungi, and plants. Because experience with voluntary registration was key to persuading mycologists of the advantages of mandatory registration, we began by asking institutions with a history of nomenclatural indexing to develop mechanisms that would permit registration. The task proved more difficult than anticipated, but considerable progress has been made, as is described in this report. It also became evident that the Nomenclature Section needs a structure that will allow ongoing discussion of registration and associated issues. Simultaneously with this report we are submitting four proposals that would provide such a structure. Fil: Barkworth, Mary E.. State University of Utah; Estados Unidos Fil: Watson, Mark. Royal Botanic Gardens; Reino Unido Fil: Barrie, Fred R.. Field Museum of National History; Estados Unidos Fil: Belyaeva, Irina V.. Royal Botanic Gardens; Reino Unido Fil: Chung, Richard C. K.. Forest Research Institute Malaysia; Malasia Fil: Dašková, Jiřina. National Museum; República Checa Fil: Davidse, Gerrit. Missouri Botanical Garden; Estados Unidos Fil: Dönmez, Ali A.. Hacettepe Üniversitesi; Turquía Fil: Alexander B. Doweld. National Institute of Carpology; Rusia Fil: Dressler, Stefan. Senckenberg Forschungsinstitut und Naturmuseum; Alemania Fil: Flann, Christina. Naturalis Biodiversity Center; Países Bajos Fil: Gandhi, Kanchi. Harvard University; Estados Unidos Fil: Geltman, Dmitry. Institute of the Russian Academy of Sciences; Rusia Fil: Glen, Hugh F.. Forest Hills; Sudáfrica Fil: Greuter, Werner. Freie Universität Berlin; Alemania Fil: Head, Martin J.. Brock University; Canadá Fil: Jahn, Regine. Freie Universität Berlin; Alemania Fil: Janarthanam, Malapati K.. Goa University; India Fil: Katinas, Liliana. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División de Plantas Vasculares; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Kirk, Paul M.. Royal Botanic Gardens; Reino Unido Fil: Klazenga, Niels. Royal Botanic Gardens; Australia Fil: Kusber, Wolf Henning. Freie Universität Berlin; Alemania Fil: Kvaček, Jiří. National Museum; República Checa Fil: Malécot, Valéry. Université d’Angers; Francia Fil: Mann, David G.. Royal Botanic Gardens; Reino Unido. Institute for Food and Agricultural Research and Technology; España Fil: Marhold, Karol. Charles University; República Checa Fil: Nagamasu, Hidetoshi. Kyoto University; Japón Fil: Nicolson, Nicky. Royal Botanic Gardens; Reino Unido Fil: Paton, Alan. Royal Botanic Gardens; Reino Unido Fil: Patterson, David J.. University of Sydney; Australia Fil: Price, Michelle J.. Conservatoire et Jardin botaniques de la Ville de Genève; Italia Fil: Prud'homme van Reine, Willem F.. Naturalis Biodiversity Center; Países Bajos Fil: Schneider, Craig W.. Trinity College; Estados Unidos Fil: Sennikov, Alexander. University of Helsinski; Finlandia Fil: Smith, Gideon F.. Nelson Mandela Metropolitan University; Sudáfrica Fil: Stevens, Peter F.. Missouri Botanical Garden; Estados Unidos. University of Missouri-St; Estados Unidos Fil: Yang, Zhu-Liang. Chinese Academy of Sciences; República de China Fil: Zhang, Xian-Chun. Chinese Academy of Sciences; República de China Fil: Zuccarello, Giuseppe C.. Victoria University of Wellington; Nueva Zelanda

European Social Fund, British Chelonia Group, North Cyprus British High Commission, North Cyprus British Residents Society, Erwin Warth Foundation, Friends of SPOT, Kuzey Kibris Turkcell and MEDASSET UK.

We report the measurement of the viscosity and density of various diesel fuels, obtained from British refineries, at elevated pressures up to 500 MPa and temperatures in the range 298 K to 373 K. The measurement and prediction procedures of fluid properties under high pressure conditions is of increasing interest in many processes and systems including enhanced oil recovery, automotive engine fuel injection, braking, and hydraulic systems. Accurate data and understanding of the fluid characteristic in terms of pressure, volume and temperature is required particularly where the fluid is composed of a complex mixture or blend of aliphatic or aromatic hydrocarbons. In this study, high pressure viscosity data was obtained using a thermostatically-controlled falling sinker-type high pressure viscometer to provide reproducible and reliable viscosity data based on terminal velocity sinker fall times. This was supported with density measurements using a micro-pVT device. Both high-pressure devices were additionally capable of illustrating the freezing points of the hydrocarbon mixtures. This work has, thus, provided data that can extend the application of mixtures of commercially available fuels and to test the validity of available predictive density and viscosity models. This included a Tait-style equation for fluid compressibility prediction. For complex diesel fuel compositions, which have many unidentified components, the approach illustrates the need to apply appropriate correlations, which require accurate knowledge or prediction of thermodynamic properties.

For many years the AGA 8 method has been the basis for all fluid property calculations related to natural gas supply, and forms an integral part of the measurement infrastructure throughout the world. However, application outside its valid range has the potential to cause significant errors. Case studies are presented that show the potential errors in density and compressibility, and resultant financial exposure, when using the AGA 8 method outside its range of viability. If all the components are within the specified composition ranges for pipeline quality gas, densities calculated using the AGA 8 method are claimed to have an uncertainty of 0.1 % (at k=2) for temperatures between -10 and 65°C at pressures up to 120 bar(a). However, for mixtures with components in the extended composition range and / or at temperatures and pressures outside the standard limits, the uncertainty increases to between 0.2 and 0.5 % (at k=2) and is effectively indeterminate for compositions outside the extended range of applicability. Comparison with values calculated using the GERG-2008 method generally confirms these claims. However, even for compositions that are within the extended AGA 8 range, the differences can exceed 1 % and the effect of ignoring pseudo-components can cause differences of over 5 %. Given the known limitations of the AGA 8 method and the lack of clarity of the likely uncertainties in calculated densities and compressibilities when used in its extended range, the use of an alternative method would be preferable. Although the GERG-2008 method overcomes some of the limitations of the AGA 8 method, there still remains the problem of ensuring that all the components in the gas stream are measured. By raising awareness of the limitations of calculating gas properties from composition, this work will help metering system designers, operators and regulators understand the issues that must be addressed when dealing with gas fields whose compositions are outside the pipeline quality gas specification required by the AGA 8 method, as the potential financial exposure can run to hundreds of millions of dollars per annum for a region such as the North Sea.

The remote monitoring of animal behaviour using telemetry and bio-logging has become popular due to technological advances, falling costs of devices and the need to understand behaviour without causing disturbance to subjects. Over the past three decades thousands of animals have had their movements tracked by these devices; however, attaching devices to streamlined bodies raises concerns about energetic costs and effects on vital rates and the reliability of the data collected (eg survival probability). We encourage researchers to discuss concerns, quantify the possible effects that devices and attachment methods have on subjects and present this work for peer review.

A hydrostatic balance densitometer with a magnetic suspension coupling has been used to measure the density of liquid toluene in the range from (293 to 373) K and pressures up to 30 MPa. New results of measurements of the density of toluene in the compressed liquid region are presented and compared with literature data. The uncertainty of the present density data is estimated to be within ± 0.01 % across the full temperature and pressure range.

Marine turtles are a taxon of world-wide conservation concern. Effective long-term monitoring is hampered by the fact that populations are widely dispersed except during the breeding season. Thus most monitoring programmes focus on nesting beaches, necessitating resource intensive studies, often over months, that could conceivably, be less comprehensive and focus on more parsimonious sampling. We analyse 11 years of exhaustive monitoring data for two species of Mediterranean marine turtles (Chelonia mydas and Caretta caretta). We resample using a variety of plausible sub-sampling regimens to estimate the total annual nesting population. We project our dataset into the future applying a range of population change rates to explore how adopting a monitoring programme based on sub-sampling would, for example, affect our ability to detect population decline. We show that accurate annual population estimates can be achieved with as few as 14 days of survey effort providing monitoring spans the peak of nesting. Furthermore, the modelled impact of sampling-based monitoring suggests that the duration to detect population change is not increased greatly. Our findings have implications for all marine turtle monitoring and have applicability to other animal groups. It is often considered desirable to perform exhaustive monitoring, with aversion of basing policy recommendations on partial data. However, comprehensive long-term monitoring programmes, particularly in developing nations, although presenting a number of advantages, are often impossible. Accurate total annual censuses can be achieved through a variety of sub-sampling regimens without sacrificing the ability to detect changes in the population trends over time. In this example, a solid block of at least 3 weeks sampling that encompasses the peak of the nesting season is advised.

Neonates with congenital cardiac disease are a special population. They are often critically ill, and need prolonged intravenous access. To date, no study has evaluated the efficacy and safety of peripherally inserted central venous catheters placed in this unique population. Our goal was to evaluate the use of such catheters in neonates with critical congenital cardiac disease, and to study features such as duration of use, reasons for removal of catheters, and complications. We inserted a total of 124 catheters in 115 neonates with critical congenital cardiac disease who were admitted to the Intensive Care Unit at Texas Children's Hospital from August 2002 to August 2004. The patients had a mean age of 10 days, and a mean weight of 3.1 kilograms. The peripherally inserted catheters were in place for a mean of 22.3 days. Therapy was completed in 76.6% patients at the time of removal of the catheter. The incidence of occlusion, dislodgement, and thrombus was 4.0%, 2.4%, and 1.6%, respectively. The infection rate was 3.6 per 1000 catheter-days, with a median onset on 37 days after placement. We conclude that central venous catheters, when inserted peripherally, provide reliable and safe access for prolonged intravenous therapy in neonates with critical congenital cardiac disease.

In species of conservation concern it is often difficult to be certain that population diver- sity and structure have been adequately characterised by genetic sampling. Since practical and financial constraints tend to be associated with increasing sample sizes in many conservation genetic studies, it is important to consider the potential for sampling error and bias due to inadequate sam- ples or spatio-temporal structure within populations. We analysed sequence data from the mitochon- drial DNA control region in a large sample (n = 245) of green sea turtles Chelonia mydas collected at the globally important rookery of Ascension Island, South Atlantic. We examined genetic diversity and structure among 10 sampling sites, 4 beach clusters and 4 nesting seasons, and evaluated the genetic composition of Ascension against other Atlantic nesting populations, including the well- studied rookery at Tortuguero (Costa Rica). Finally, we used rarefaction and GENESAMP analyses to assess the ability of different sample sizes to provide acceptable genetic representations of a popula- tion, using Ascension and Tortuguero as models. On Ascension, we found 13 haplotypes, of which only 3 had been previously observed in the rookery, and 5 previously undescribed. We detected no differentiation among beach clusters or sampling seasons, and only weak differentiation among the 3 primary nesting sites. The increased sample size for Ascension provided higher resolution and statistical power in describing genetic structure among all other known Atlantic rookeries. Our extrapolations showed that a maximum of 18 and 6 haplotypes are expected to occur in Ascension and Tortuguero, respectively, and that current sample sizes are sufficient to describe most of the vari- ation. We recommend using rarefaction and GENESAMP analyses on a rookery-by-rookery basis to evaluate whether a sample set adequately describes mitochondrial DNA diversity, thus strengthen- ing subsequent phylogeographic and mixed stock analyses, and management recommendations for conservation.