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Aim: The aim of this study was to analyze the endothelial cell count and morphology of the endothelial cell of donor corneas using Eye Bank Specular Microscope (EBSM). Materials and Methods: Eye bank data of 100 donor corneas procured and processed between January 2016 and December 2017, at an eye bank associated with a medical college hospital were retrospectively analyzed. Endothelial cell density (ECD), coefficient of variance (CV), and percentage age of hexagonality (6A) of donor cornea were compared across different age groups, gender, and phakic status of the donor tissue. Results: The mean age of the donors was 63.53 ± 19.92 (range 15–92 years). The mean ECD of the donor cornea was 2708.93 ± 271.52 cells/mm2; the average CV was 28.37 ± 6.3; and the average percentages of hexagonal cells were 63.85%. The cell count was significantly greater in the age group of