1. Single-cell transcriptomic analysis of radiation-induced lung injury in rat.
- Author
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Shi XY, Zhu YQ, Liang CJ, Chen T, Shi Z, and Wang W
- Subjects
- Animals, Rats, Transcriptome radiation effects, Lung pathology, Lung radiation effects, Lung metabolism, Reactive Oxygen Species metabolism, Radiation Injuries, Experimental metabolism, Radiation Injuries, Experimental pathology, Radiation Injuries, Experimental genetics, Gene Expression Profiling methods, Myocytes, Smooth Muscle metabolism, Myocytes, Smooth Muscle radiation effects, Myocytes, Smooth Muscle pathology, Male, Radiation Injuries pathology, Radiation Injuries genetics, Radiation Injuries metabolism, Extracellular Matrix metabolism, Extracellular Matrix radiation effects, Rats, Sprague-Dawley, Lung Injury etiology, Lung Injury genetics, Lung Injury metabolism, Lung Injury pathology, Single-Cell Analysis methods
- Abstract
Radiation-induced lung injury (RILI) frequently occurs as a complication following radiotherapy for chest tumors like lung and breast cancers. However, the precise underlying mechanisms of RILI remain unclear. In this study, we generated RILI models in rats treated with a single dose of 20 Gy and examined lung tissues by single-cell RNA sequencing (scRNA-seq) 2 weeks post-radiation. Analysis of lung tissues revealed 18 major cell populations, indicating an increase in cell-cell communication following radiation exposure. Neutrophils, macrophages, and monocytes displayed distinct subpopulations and uncovered potential for pro-inflammatory effects. Additionally, endothelial cells exhibited a highly inflammatory profile and the potential for reactive oxygen species (ROS) production. Furthermore, smooth muscle cells (SMC) showed a high propensity for extracellular matrix (ECM) deposition. Our findings broaden the current understanding of RILI and highlight potential avenues for further investigation and clinical applications.
- Published
- 2024
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