1. Dentate gyrus and hilus transection blocks seizure propagation and granule cell dispersion in a mouse model for mesial temporal lobe epilepsy
- Author
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Sophie Hamelin, Antoine Depaulis, Colin Deransart, Johan Pallud, Ute Häussler, Mélanie Langlois, Bertrand Devaux, Service de neurochirurgie, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Descartes - Paris 5 (UPD5)-Centre Hospitalier Sainte-Anne, Experimental Epilepsy, Neurocenter, University of Freiburg [Freiburg], Grenoble Institut des Neurosciences (GIN), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), Deransart, Colin, Assistance publique - Hôpitaux de Paris (AP-HP) - Université Paris Descartes - Paris 5 (UPD5) - Centre Hospitalier Sainte-Anne, and Université Joseph Fourier - Grenoble 1 (UJF) - CHU Grenoble - Institut National de la Santé et de la Recherche Médicale (INSERM)
- Subjects
Male ,Kainic acid ,Cognitive Neuroscience ,Hippocampus ,Hippocampal formation ,Biology ,Epileptogenesis ,Neurosurgical Procedures ,03 medical and health sciences ,Epilepsy ,chemistry.chemical_compound ,Mice ,0302 clinical medicine ,Seizures ,medicine ,Animals ,[SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] ,030304 developmental biology ,Neurons ,0303 health sciences ,Kainic Acid ,Dentate gyrus ,Limbic lobe ,medicine.disease ,Granule cell dispersion ,Mice, Inbred C57BL ,Disease Models, Animal ,chemistry ,Epilepsy, Temporal Lobe ,nervous system ,Dentate Gyrus ,Mossy Fibers, Hippocampal ,[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] ,Neuroscience ,030217 neurology & neurosurgery - Abstract
International audience; Epilepsy-associated changes of the anatomical organization of the dentate gyrus and hilus may play a critical role in the initiation and propagation of seizures in mesial temporal lobe epilepsy (MTLE). This study evaluated the role of longitudinal projections in the propagation of hippocampal paroxysmal discharges (HPD) in dorsal hippocampus by performing a selective transection in a mouse model for MTLE obtained by a single unilateral intrahippocampal injection of kainic acid (KA). Full transections of the dentate gyrus and hilus were performed in the transverse axis at 22 days after KA injection when spontaneous HPD were fully developed. They: (i) significantly reduced the occurrence of HPD; (ii) increased their duration at the KA injection site; (iii) abolished their spread along the longitudinal axis of the hippocampal formation and; (iv) limited granule cell dispersion (GCD) of the dentate gyrus posterior to the transection. These data suggest that: (i) longitudinal projections through the dentate gyrus and hilus are involved in HPD spread; (ii) distant hippocampal circuits participate in the generation and cessation of HPD and; (iii) GCD requires continuous HPD to develop, even when seizures are established. Our data reveal a critical role for longitudinal projections in the generation and spread of hippocampal seizures. © 2010 Wiley-Liss, Inc.
- Published
- 2010