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Adolescent traumatic brain injury (TBI) has long-term effects on brain functioning and behavior, impacting neural activity under cognitive load, especially in the reward network. Adolescent TBI is also linked to risk-taking behaviors including alcohol misuse. It remains unclear how TBI and neural functioning interact to predict alcohol experimentation during adolescence. Using Adolescent Brain Cognitive Development (ABCD) study data, this project examined if TBI at ages 9–10 predicts increased odds of alcohol sipping at ages 11–13 and if this association is moderated by neural activity during the Emotional EN-Back working memory task at ages 11–13. Logistic regression analyses showed that neural activity in regions of the fronto-basal ganglia network predicted increased odds of sipping alcohol by ages 11–13 (p

Objectives To determine whether a brief self-directed forgiveness workbook intervention could alter forgiveness, depression symptoms, and anxiety symptoms.Design A multisite randomised waitlist-controlled trial was conducted among 4598 participants. Recruitment occurred from 11 February 2020 to 30 September 2021. Final follow-up occurred on 25 October 2021.Setting Participants were recruited from community-based samples in sites in Colombia, Hong Kong, Indonesia, South Africa, and Ukraine.Participants Individuals (n=7837) were screened for eligibility. For inclusion, participants needed to be ≥18 years and have experienced an interpersonal transgression. The analytic sample consisted of n=4598 participants, median age 26 and 73% female.Interventions At each site, participants were randomly assigned to either immediate receipt of a self-directed forgiveness workbook intervention, or to receipt after a 2 week delay.Main outcomes measures The primary outcomes were unforgiveness (Transgression-Related Interpersonal Motivations Inventory-18), depression symptoms, and anxiety symptoms (Brief Symptom Inventory-18) measured at 2 weeks following intervention assignment.Results At 2 weeks follow-up, unforgiveness was lower among the immediate-treatment group compared with the delayed-treatment group (standardised mean difference=−0.53 (95% CI=−0.58 to –0.47)); similar patterns were found for depression (standardised mean difference=−0.22 (95% CI=−0.28 to –0.16)) and anxiety symptoms (standardised mean difference=−0.21 (95% CI=−0.27 to –0.15)).Conclusions A brief workbook intervention promoted forgiveness and reduced depression and anxiety symptoms. The promotion of forgiveness with such workbooks has the potential for widespread dissemination to improve global mental health.Trial registration number NCT04257773.

Objectives: To evaluate the effectiveness of a forgiveness public health intervention at promoting forgiveness, mental health, and flourishing.Methods: Colombian students (N = 2,878) at a private, nonreligious university were exposed to a 4-week forgiveness community campaign and were assessed pre- and post-campaign.Results: Forgiveness, mental health, and flourishing outcomes showed improvements after the campaign. On average, participants reported engaging in 7.18 (SD = 3.99) of the 16 types of campaign activities. The number of types of campaign activities that participants engaged in evidenced a positive linear association with forgiveness, although some activities were more popular than others and some activities were more strongly associated with increased forgiveness. For depression, anxiety, and flourishing, engaging in more activities was generally associated with greater improvements, but the patterns were less consistent relative to forgiveness.Conclusion: This forgiveness public health intervention effectively promoted forgiveness, mental health, and flourishing. Effective campaigns in diverse communities involve promoting mental and physical health through forgiveness. However, recent conflict may hinder acceptance, necessitating political capital for leadership advocating forgiveness initiatives.

The present study focused on the roles of self-forgiveness and feeling divine forgiveness in mitigating the adverse effects of stress on substance use cravings in Trinidad and Tobago. We assessed 869 individuals (62 percent of whom were women) through self-report online questionnaires. A moderation analysis using the PROCESS macro and a bootstrapping strategy (N = 5000) revealed that greater levels of self-forgiveness weakened the positive relationship between stress and substance use cravings, though the relationship remained significant. This moderating effect was evident only when individuals reported high levels of feeling divine forgiveness. The findings suggest that both forms of forgiveness lessen the impact of stress on substance use cravings, highlighting their potential as protective factors and underscoring the importance of incorporating religious and spiritual dimensions into psychological education and intervention. However, the study’s cross-sectional nature makes it difficult to make causal inferences, indicating a need for longitudinal research.

Much attention has been devoted to the effectiveness of forgiveness interventions in children and adolescents featuring two premier programs by Enright and his colleagues. Little attention has been given to psychoeducational forgiveness interventions with emerging adults. This is a narrative review of REACH Forgiveness studies with emerging adults (ages 18–25). The life tasks of emerging adults justify offering psychoeducational interventions to emerging adults. Research studies on REACH Forgiveness (k = 17), non-REACH Forgiveness studies (k = 4), and community campaigns at universities (k = 4) with emerging adults are summarized. Effect sizes per hour (d/h) for REACH Forgiveness studies (k = 13 for psychoeducational groups; k = 4 for self-administered workbooks) are reported. The proto-REACH groups (k = 5) had mean d/h = 0.104; REACH groups (k = 9) had d/h = 0.101; self-administered workbooks (k = 3) had mean d/h = 0.15; non-REACH Forgiveness studies (k = 4) had d/h = 0.09. All studies were from the USA, and most were from universities. However, a recent article reported randomized controlled trials in five non-USA samples of adults (N = 4598). A 3.34-h workbook had d/h = 0.16, suggesting that the workbook might be effective with emerging adults around the world. Finally, three USA Christian universities had public health immersion campaigns to promote forgiveness, and a community psychoeducational campaign in 2878 secular university students in Colombia (of ~9000 total) allowed choices among 16 psychoeducational activities. The number of activities used was proportional to forgiveness experienced. For forgiveness, d = 0.36 plus substantial reductions in depression and anxiety, indicating strong public health potential of forgiveness psychoeducation in emerging adults worldwide.

Objective: Breast cancer is the most commonly diagnosed cancer in women worldwide, and its incidence has increased recently. Diagnosing cancer can create many challenges, especially for married women. The aim of the present study was thus to evaluate the effect of the relationships among marital empathy, body image, and perceived social support on quality of life (QoL) and the mediating role of perceived marital quality. Method: Married women with breast cancer (N = 160) were selected through purposive accessible sampling. Measures included body image scale, Batson empathy adjectives, multidimensional scale of perceived social support, perceived marital quality, and QoL. The research method was descriptive-correlational and using structural equation modeling. Results: The best model obtained showed that all predictors directly predicted QoL: marital empathy, body image, and perceived social support (P < 0.001). Predictors (except for perceived social support by family, friends, and others) also worked indirectly through perceived marital quality to predict QoL (P < 0.001). Conclusion: Several variables predicted QoL for women with breast cancer, including body image and marital empathy. Most of such variables had both a direct effect and an indirect effect, working through perceived marital quality to affect QoL. Social support, however, had only a direct effect on QoL.

Abstract Several moons in the outer solar system host liquid water oceans. A key next step in assessing the habitability of these ocean worlds is to determine whether life’s elemental and energy requirements are also met. Phosphorus is required by all known life and is often limited to biological productivity in Earth’s oceans. This raises the possibility that its availability may limit the abundance or productivity of Earth-like life on ocean worlds. To address this potential problem, here we calculate the equilibrium dissolved phosphate concentrations associated with the reaction of water and rocks—a key driver of ocean chemical evolution—across a broad range of compositional inputs and reaction conditions. Equilibrium dissolved phosphate concentrations range from 10−11 to 10−1 mol/kg across the full range of carbonaceous chondrite compositions and reaction conditions considered, but are generally > 10−5 mol/kg for most plausible scenarios. Relative to the phosphate requirements and uptake kinetics of microorganisms in Earth’s oceans, such concentrations would be sufficient to support initially rapid cell growth and construction of global ocean cell populations larger than those observed in Earth’s deep oceans.

IntroductionElevated levels of behavioral inhibition (BI) may connote risk for both anxiety and substance use disorders. BI has consistently been shown to be associated with increased levels of anxiety, while the association between BI and substance use has been mixed. It is possible that the relationship between BI and substance use varies by individual difference factors. Hispanic/Latinx (H/L) youth in particular may have stronger relationships between BI, anxiety, and substance use.MethodsThe present study therefore evaluated (1) the prospective relationships between BI [assessed via self-reported behavioral inhibition system (BIS) scale scores], anxiety, and substance use in youth (n = 11,876) across baseline, 1-, and 2-year follow-ups of the Adolescent Brain Cognitive Development (ABCD) Study (ages 9–12) and (2) whether these relationships differed by H/L ethnicity while covarying for average behavioral approach system scores, race, sex, age, highest parental income, highest parental education, and past-year substance use (for analyses involving substance use outcomes).ResultsBaseline levels of BIS scores predicted increased anxiety symptoms at both 1- and 2-year follow-ups and did not differ by H/L ethnicity. Baseline levels of BIS scores also prospectively predicted increased likelihood of substance use at 2-year follow-up, but only for H/L youth and not at 1-year follow-up.DiscussionHigh scores on the BIS scale contribute risk to anxiety across ethnicities and may uniquely contribute to risk for substance use in H/L youth.

In the Samail Ophiolite of Oman, the geological process of serpentinization produces reduced, hydrogen rich, hyperalkaline (pH > 11) fluids. These fluids are generated through water reacting with ultramafic rock from the upper mantle in the subsurface. On Earth’s continents, serpentinized fluids can be expressed at the surface where they can mix with circumneutral surface water and subsequently generate a pH gradient (∼pH 8 to pH > 11) in addition to variations in other chemical parameters such as dissolved CO2, O2, and H2. Globally, archaeal and bacterial community diversity has been shown to reflect geochemical gradients established by the process of serpentinization. It is unknown if the same is true for microorganisms of the domain Eukarya (eukaryotes). In this study, using 18S rRNA gene amplicon sequencing, we explore the diversity of microbial eukaryotes called protists in sediments of serpentinized fluids in Oman. We demonstrate that protist community composition and diversity correlate significantly with variations in pH, with protist richness being significantly lower in sediments of hyperalkaline fluids. In addition to pH, the availability of CO2 to phototrophic protists, the composition of potential food sources (prokaryotes) for heterotrophic protists and the concentration of O2 for anaerobic protists are factors that likely shape overall protist community composition and diversity along the geochemical gradient. The taxonomy of the protist 18S rRNA gene sequences indicates the presence of protists that are involved in carbon cycling in serpentinized fluids of Oman. Therefore, as we evaluate the applicability of serpentinization for carbon sequestration, the presence and diversity of protists should be considered.

Hot spring outflow channels provide geochemical gradients that are reflected in microbial community compositions. In many hot spring outflows, there is a distinct visual demarcation as the community transitions from predominantly chemotrophs to having visible pigments from phototrophs. It has been hypothesized that this transition to phototrophy, known as the photosynthetic fringe, is a result of the pH, temperature, and/or sulfide concentration gradients in the hot spring outflows. Here, we explicitly evaluated the predictive capability of geochemistry in determining the location of the photosynthetic fringe in hot spring outflows. A total of 46 samples were taken from 12 hot spring outflows in Yellowstone National Park that spanned pH values from 1.9 to 9.0 and temperatures from 28.9 to 92.2°C. Sampling locations were selected to be equidistant in geochemical space above and below the photosynthetic fringe based on linear discriminant analysis. Although pH, temperature, and total sulfide concentrations have all previously been cited as determining factors for microbial community composition, total sulfide did not correlate with microbial community composition with statistical significance in non-metric multidimensional scaling. In contrast, pH, temperature, ammonia, dissolved organic carbon, dissolved inorganic carbon, and dissolved oxygen did correlate with the microbial community composition with statistical significance. Additionally, there was observed statistical significance between beta diversity and the relative position to the photosynthetic fringe with sites above the photosynthetic fringe being significantly different from those at or below the photosynthetic fringe according to canonical correspondence analysis. However, in combination, the geochemical parameters considered in this study only accounted for 35% of the variation in microbial community composition determined by redundancy analysis. In co-occurrence network analyses, each clique correlated with either pH and/or temperature, whereas sulfide concentrations only correlated with individual nodes. These results indicate that there is a complex interplay between geochemical variables and the position of the photosynthetic fringe that cannot be fully explained by statistical correlations with the individual geochemical variables included in this study.

Throughout the Archean, H2 generation via low-temperature ultramafic serpentinization likely helped prevent atmospheric O2 accumulation and continued until the abundance of ultramafic rocks diminished setting the stage for the Great Oxidation Event.

Research has shown that people sometimes report self-perceived growth as a result of dealing with a potentially traumatic event, but relatively few methodologically rigorous studies have examined whether perceived posttraumatic growth is associated with improved subsequent well-being across a wide range of outcomes. In this three-wave longitudinal study of Colombian emerging adults (n = 636), we examined the associations of perceived posttraumatic growth with 17 well-being outcomes across domains of psychological well-being (i.e., self-rated mental health, meaning in life, sense of purpose, happiness, life satisfaction), psychological distress (i.e., anxiety symptoms, depression symptoms, subjective suffering), social well-being (i.e., content with relationships, satisfying relationships, loneliness), physical well-being (i.e., self-rated physical health, sleep quality), and character strengths (i.e., state hope, trait forgivingness, orientation to promote good, delayed gratification). Using an outcome-wide analytic design that adjusted for a range of covariates assessed in Wave 1, we found that overall perceived posttraumatic growth assessed in Wave 2 was robustly associated with improvements in one or more facet of each well-being domain (15/17 outcomes in total) assessed approximately six months later in Wave 3. Our findings suggest that perceived posttraumatic growth may contribute to individual well-being over the longer-term.

Research suggests that interpersonal forgiveness is beneficial to individual functioning, but few longitudinal studies have explored the independent contributions of decisional and emotional forgiveness to reducing different forms of distress and improving multidimensional well-being. In this three-wave (T1: December 2020; T2: January 2021; T3: February 2021) prospective study of predominantly young Indonesian adults (n = 595), we examined the associations of decisional and emotional forgiveness with three indicators of distress and 10 components of well-being. Applying the outcome-wide analytic template for longitudinal designs, our primary analysis involved estimating two sets of linear regression models (one set for decisional forgiveness and one set for emotional forgiveness) in which the outcomes were regressed on each interpersonal forgiveness process (one outcome at a time). Adjusting for a range of covariates (including prior values of decisional forgiveness, emotional forgiveness, and all 13 outcomes) assessed at T1, decisional forgiveness assessed at T2 was associated with an increase in seven components of well-being (i.e., life satisfaction, physical health, sense of purpose, promote good, delayed gratification, content with relationships, satisfying relationships) approximately 1 month later at T3. In contrast, emotional forgiveness assessed at T2 was associated with an increase in a single component of well-being (i.e., satisfying relationships) assessed at T3. Neither decisional nor emotional forgiveness assessed at T2 showed evidence of associations with any of the subsequent indicators of distress assessed at T3. Our findings suggest that, at least within a principally collectivistic cultural context such as Indonesia, decisional forgiveness in the aftermath of a transgression may have greater short-term benefits for well-being compared to emotional forgiveness. Implications of the findings for research and interventions are discussed.

Abstract Background Forgiveness is a concept of growing interest within psychology and of potential relevance to public health. While there has been increasing evidence suggesting positive associations between forgiveness of others and a range of psychosocial well-being and mental health outcomes, its associations with health behaviors and physical health are less clear. Methods This study used longitudinal data from the Nurses’ Health Study II (2008 Trauma Exposure and Post-traumatic Stress Supplementary Survey to 2015 questionnaire wave, N = 54,703), to conduct an outcome-wide analysis among a cohort of female nurses in the United States (age range: 43–64 years). The study prospectively examines the association between spiritually motivated forgiveness of others and a number of of subsequent psychosocial well-being, mental health, health behavior, and physical health outcomes in midlife. A set of linear, logistic, and Poisson regression models were used to regress each outcome on forgiveness in separate models. Sociodemographic factors, prior religious service attendance, and prior values of all outcome variables were controlled for wherever data were available. To account for multiple testing, we performed Bonferroni correction. Results Forgiveness was associated with subsequent improved psychosocial well-being and reduced psychological distress outcomes in a monotonic pattern. For instance, the top versus bottom level of forgiveness was associated with substantially higher levels of subsequent positive affect (β = 0.18, 95% CI: 0.15, 0.21) and social integration (β = 0.15, 95% CI: 0.13, 0.17), and was inversely associated with several indicators of subsequent psychological distress such as depressive symptoms (β = − 0.16, 95% CI: − 0.19, − 0.14). However, in this sample, there was little evidence that forgiveness was associated with health behaviors or physical health outcomes. Discussion This study suggests that forgiveness may be a health asset for promoting population mental health and psychosocial well-being, and moreover may also be understood as a good in itself. Further investigation on the dynamics between forgiveness and physical health is warranted to explore the discrepancy between the results here and some past research.

Positive psychology has accumulated a large and ever-growing body of scientific knowledge about human strengths and virtues. However, research on positive psychology interventions (PPIs) to develop such is still in its infancy. In this brief position paper, we summarize the status of PPIs in one of the positive psychology’s most important virtues: temperance. Temperance refers to the capacity to manage habits and protect against excess and is composed of forgiveness, humility, and (we include) patience. Specifically, we examine the current state-of-the-science in the conceptualization of temperance, explore the efficacy of temperance interventions, and reflect upon what the future may hold in this research domain. In this paper, we first highlight the challenges and opportunities for expanding the theoretical conceptualization of temperance and reflect upon the challenges in temperance-related PPIs. For each aspect of temperance, we propose a specific research agenda. Second, we explore what is needed for PPIs to promote temperance and how growth in temperance intervention research can be fostered. Generally, while forgiveness interventions are well established, we recommended that both humility and patience interventions need more viable evidence-based research on existing and new interventions. Third, we advanced several recommendations regarding how to promote more research in new interventions. These recommendations included attracting more funders to the area, developing new interventions, and employing new technology. Because intervention research in temperance is in its infancy, the future looks rosy for PPI researchers as we move into a second generation of positive psychology research.

Within the winner or loser dichotomy that exemplifies competitive sport, athletic success hinges on the ability to overcome and respond constructively to failure. This article introduces self-forgiveness as an adaptive, purposeful approach to coping with competitive sport performance failure in a way that stimulates personal growth and combats loss of motivation. In contrast to defensive responses that shield self-integrity, genuine self-forgiveness is reached through a process in which athletes (a) accept personal responsibility for their role in the unsuccessful performance outcome and (b) restore self-regard by affirming the self. Although athletes expose themselves to uncomfortable emotional experiences associated with failure, self-forgiveness is proposed as a process that enables athletes to objectively evaluate unsuccessful performances, identify areas warranting improvement, and develop adaptive psychological recovery responses to failure.

Several studies have suggested that self-forgiveness promotes psychological well-being. The state self-forgiveness scale (SSFS) and the differentiated self-forgiveness process scale (DSFPS) are two self-report questionnaires that assess self-forgiveness in psychotherapy, personal change, and health. The present study aims to examine the psychometric properties of the SSFS and the DSFPS in a Portuguese sample, highlighting reliability and validity properties for scores on both scales. We examine the relationships among self-forgiveness, self-criticism, psychological well-being, and global psychopathological symptoms. The two scales were completed in a random nonclinical sample of 475 University students. The psychological well-being scale was used to explore the relation between self-forgiveness and well-being. Our findings show evidence of a good estimated internal consistency for scores on both scales - SSFS and DSFPS. Self-forgiveness is related to higher indexes of positive feelings (e.g., self-compassion, self-esteem) such as positive behaviors and beliefs about the self. Thus, self-criticism (e.g., hated self) tends to decrease while self-forgiveness feelings and actions, as well as positive feelings of the self (self-compassion; self-love) tends to increase. In clinical practice these are positive indicators, which can lead to personal improvement, as well as positive affect and lower symptomatology (e.g., symptoms of depression).

Suffering has been a topic of considerable discussion in the fields of medicine and palliative care, yet few studies have reported causal evidence linking the experience of suffering to health and well-being. In this three-wave prospective cohort study, we explore the potential psychological implications of suffering during the COVID-19 pandemic by examining relations among suffering, mental health, and psychological well-being in a sample of U.S. adults living with chronic health conditions. We analyzed data from n = 184 participants who completed assessments one month before the SARS-CoV-2 outbreak was declared a pandemic by the World Health Organization (February 2020) and then two months (April 2020) and four months later (May/June 2020). Analyses controlled for a range of factors, including sociodemographic characteristics, physical health, religious/spiritual factors, psychological characteristics, and prior values of the predictor and each of the outcomes assessed one month before the COVID-19 pandemic. Results of the primary analysis indicated that greater overall suffering assessed one month into the COVID-19 pandemic was associated with lower psychological well-being (β = -.17, 95% CI: -.29, -.05) and higher levels of anxiety (β = .27, 95% CI: .13, .41) and depression (β = .16, 95% CI: .03, .29) two months later. In a secondary analysis that explored anxiety, depression, and psychological well-being as candidate antecedents of suffering, depression assessed one month into the COVID-19 pandemic was most strongly associated with worse overall suffering two months later. We highlight the implications of the findings for high-risk populations who are suffering amidst the challenges of the COVID-19 pandemic. Potential benefits of both integrating assessments of suffering into screening procedures and addressing experiences of suffering in mental health service settings are discussed.

Hope is a topic widely discussed in the humanities and researched in the field of psychology. To explore the potential public health implications of hope for subsequent health and well-being outcomes, we prospectively examined the relation between baseline hope and a wide range of outcomes that included indicators of: physical health, health behaviors, and psychosocial well-being in older adults using an outcome-wide approach. Data from the Health and Retirement Study (N = 12,998, mean age = 66 years) were analyzed. Bonferroni correction was used to account for multiple testing. All models controlled for a wide array of factors including: sociodemographic characteristics, personality factors, and prior values of the exposure (hope) and all outcomes. A greater sense of hope was associated with: better physical health and health behavior outcomes on some indicators (e.g., reduced risk of all cause-mortality, fewer number of chronic conditions, lower risk of cancer, and fewer sleep problems), higher psychological well-being (e.g., increased positive affect, life satisfaction, and purpose in life), lower psychological distress, and better social well-being. A secondary analysis explored antecedents of hope. We identified several potentially modifiable factors that may lead to increased hope. These results may have important population-level implications for increasing hope and improving the physical, psychological, and social well-being of our growing older adult population.

The present study investigated the efficacy of the REACH Forgiveness psychoeducation program for the first time in Indian college students and examined theoretically-based predictors of program response based on the model of relational spirituality and forgiveness. This was an intervention experiment that spanned 5 weeks and included three measurement occasions (weeks 1, 3, 5) and two separate deliveries of the forgiveness intervention (weeks 2 and 4). Participants were N = 124 students at Karnatak University in Darwha, India (100 Hindu; 18 Muslim, 5 Christian, and 1 Jain). This was a manualized, secular intervention led by a trained facilitator in a group, psychoeducational format. Measures included forgiveness and unforgiveness as well as assessments of positive and negative affective states and spirituality. Participants who received immediate forgiveness training showed significant and large positive changes in forgiveness and unforgiveness, as well as, more positive affect and increased self-esteem in contrast to wait-list comparisons. Perceiving one’s offender as having a similar spirituality to oneself was a consistent predictor of response to the REACH Forgiveness program. Specifically, perceiving the offender as having a similar spirituality was related to less growth of unforgiveness and more growth in empathy, positive affect, and emotional forgiveness as a result of the psychoeducational program. The REACH Forgiveness psychoeducational approach is efficacious in an Indian college student sample, and some relational spirituality variables are important predictors of response to the program. Future studies should consider the role of Indian culture in promoting forgiveness and possibly tailor the intervention to suit the significant proportions of Hindus and Muslims in India.

Apology and restitution each represents wrongdoers’ accountable repair responses that have promoted victims’ self-reported empathy and forgiveness in crime scenario research. The current study measured emotional and stress-related dependent variables including physiological measures, to illuminate the links between predictors of forgiveness and health-relevant side effects. Specifically, we tested the independent and interactive effects of apology and restitution on forgiveness, emotion self-reports, and facial responses, as well as cardiac measures associated with stress in 32 males and 29 females. Apology and restitution each independently increased empathy, forgiveness, gratitude, and positive emotions, while reducing unforgiveness, negative emotion, and muscle activity above the brow (corrugator supercilii, CS). The presence of a thorough apology—regardless of whether restitution was present—also calmed heart rate, reduced rate pressure products indicative of cardiac stress, and decreased muscle activity under the eye (orbicularis oculi, OO). Interactions pointed to the more potent effects of restitution compared to apology for reducing unforgiveness and anger, while elevating positivity and gratitude. The findings point to distinctive impacts of apology and restitution as factors that foster forgiveness, along with emotional and embodied changes relevant to health.

The influence of oxidation-reduction (redox) potential on the expression of biomolecules is a topic of ongoing exploration in geobiology. In this study, we investigate the novel possibility that structures and compositions of lipids produced by microbial communities are sensitive to environmental redox conditions. We extracted lipids from microbial biomass collected along the thermal and redox gradients of four alkaline hot springs in Yellowstone National Park (YNP) and investigated patterns in the average oxidation state of carbon (ZC), a metric calculated from the chemical formulae of lipid structures. Carbon in intact polar lipids (IPLs) and their alkyl chains becomes more oxidized (higher ZC) with increasing distance from each of the four hot spring sources. This coincides with decreased water temperature and increased concentrations of oxidized inorganic solutes, such as dissolved oxygen, sulfate, and nitrate. Carbon in IPLs is most reduced (lowest ZC) in the hot, reduced conditions upstream, with abundance-weighted ZC values between −1.68 and −1.56. These values increase gradually downstream to around −1.36 to −1.33 in microbial communities living between 29.0 and 38.1°C. This near-linear increase in ZC can be attributed to a shift from ether-linked to ester-linked alkyl chains, a decrease in average aliphatic carbons per chain (nC), an increase in average degree of unsaturation per chain (nUnsat), and increased cyclization in tetraether lipids. The ZC of lipid headgroups and backbones did not change significantly downstream. Expression of lipids with relatively reduced carbon under reduced conditions and oxidized lipids under oxidized conditions may indicate microbial adaptation across environmental gradients in temperature and electron donor/acceptor supply.

Adolescent traumatic brain injury (TBI) has long-term effects on brain functioning and behavior, impacting neural activity under cognitive load, especially in the reward network. Adolescent TBI is also linked to risk-taking behaviors including alcohol misuse. It remains unclear how TBI and neural functioning interact to predict alcohol experimentation during adolescence. Using Adolescent Brain Cognitive Development (ABCD) study data, this project examined if TBI at ages 9-10 predicts increased odds of alcohol sipping at ages 11-13 and if this association is moderated by neural activity during the Emotional EN-Back working memory task at ages 11-13. Logistic regression analyses showed that neural activity in regions of the fronto-basal ganglia network predicted increased odds of sipping alcohol by ages 11-13 (p

In Ghana, collectivism holds people together in marital relationships, even if partners are religiously different. Married partners still hurt, betray, or offend each other and might develop avoidance or vengeful (i.e., unforgiving) motives. We investigated whether religious homogamy moderated connections of personality and marriage variables to unforgiving motives. Heterosexual married couples (N = 176 heterosexual married couples; N = 352 individuals; mean marriage duration 10.89 years) participated. Most identified as Christian (83.5% males; 82.3% females) or Muslim (11.9% males; 14.3% females). Couple religious homogamy was related directly to lower unforgiving motives. Religious homogamy did not moderate the connection between some personality variables (i.e., agreeableness and trait forgivingness) and unforgiving motives. Religiously unmatched couples tended to have greater unforgiveness at higher levels of neuroticism and lower forbearing, marital satisfaction, and marital commitment relative to religiously matched couples. One implication is that couple therapists need to assess partner neuroticism, marriage climate (i.e., satisfaction and commitment), and the general tendency to forbear when offended. Those can combine to produce unforgiving relationships, which might make progress in couple therapy improbable.

Forgiveness frequently occurs in a relational context and is a key ingredient for restoring and maintaining intimate relationships. Yet, certain interpersonal dynamics that sometimes motivate forgiveness (e.g. abuse) have the potential to adversely affect well-being, especially when ongoing exploitation occurs. In this study, we examined the role of forgiveness in moderating relations between psychological abuse and indicators of psychological distress in a sample of community-based South African women currently in a heterosexual romantic relationship. Participants (n=515) completed measures of decisional and emotional forgiveness of their partner, psychological abuse committed by their current partner during the course of the relationship, and depression, anxiety, and stress. Latent profile analysis identified two subgroups characterised by differing levels of forgiveness: partial forgiveness (high decisional forgiveness and moderate emotional forgiveness) and complete forgiveness (high decisional and emotional forgiveness). Regression analyses revealed that the relations of psychological abuse with depression and stress, but not anxiety, were moderated by ‘forgiveness of partner’. The complete forgiveness group scored lower on depression and stress when psychological abuse was lower, but higher on each outcome when psychological abuse was higher. The findings suggest that there may be conditions in which forgiveness of partner may promote or undermine the mental health of women who experience abuse perpetrated by their current partner. Significance: • Whereas women in continuing romantic relationships generally sought neither to avoid or seek revenge on their partners (i.e. decisional forgiveness), distinct subgroups were characterised by more or less reduction of negative emotions (i.e. emotional forgiveness). • Within the context of continuing romantic relationships, the mental health benefits that ordinarily accompany more thorough processing of unforgiveness may be eroded when victims are exposed to severe levels of potentially ongoing psychological abuse.

Forgiveness is a central theme within the Christian faith, yet Christian traditions sometimes vary in how they understand and approach the forgiveness process. Nevertheless, in this paper, we present an ecumenical model of Christian forgiveness that highlights the essential components that are shared across most Christian traditions. Importantly, rather than using a theological lens to develop and describe this model, we have primarily used a psychological lens. Specifically, we have adopted stress-and-coping theory as the psychological framework for understanding a Christian conceptualization of forgiveness. We identify four types of forgiveness (divine forgiveness, self-forgiveness, person-to-person forgiveness, and organizational–societal forgiveness) and describe a Christian conceptualization of each one, filtered through the psychological perspective of stress-and-coping theory.

Theologians, pastors, and psychological help-providers have not always worked harmoniously. This can be especially true with couples. Theological and pastoral help-providers value marriage as sacred and are reluctant to entertain ending it. Most psychotherapists have more training and experience in individual psychotherapy than in couple therapy. Drawing on the parable of the Good Samaritan, we appeal to theologians, pastors, and psychological help-givers to work together. We examine ways that psychological findings might inform theology and pastoral practice. As an example, we use forgiveness in committed romantic relationships. What causes strong couple relationships are the formation, strengthening, maintenance, and (when damaged) repair of ruptures in the emotional bond. Thus, forgiveness is one major cause of good marriage. Forgiveness requires being oriented toward the other person’s welfare, and in humility responding to wrongdoing mercifully. Forgiving in committed relationships seeks a net positive emotional valence toward the partner built on empathy, humility, and responsibility. Good relationships also involve self-forgiveness when one feels self-condemnation over one’s own misdeeds. For help-givers, humility is a key to promoting relational experiences of virtue. We show that forgiveness is related to health. Religiously oriented help-providers can promote better relationships and better health by fostering forgiveness.

Gas seeps emanating from ophiolites at Yanartaş (Chimaera), Turkey, have been documented for thousands of years. Active serpentinization produces hydrogen and a range of carbon gases that may provide fuel for life. Here we report a newly discovered, ephemeral fluid seep emanating from a small gas vent at Yanartaş. Fluids and biofilms were sampled at the source and points downstream. We describe site conditions, and provide microbiological data in the form of enrichment cultures, scanning electron microscopy (SEM), carbon and nitrogen isotopic composition of solids, and PCR screens of nitrogen cycle genes. Source fluids are pH 11.95, with a Ca:Mg of ~200, and sediments under the ignited gas seep measure 60°C. Collectively, these data suggest the fluid is the product of active serpentinization at depth. Source sediments are primarily calcite and alteration products (chlorite and montmorillonite). Downstream, biofilms are mixed with montmorillonite. SEM shows biofilms distributed homogeneously with carbonates. Organic carbon accounts for 60% of the total carbon at the source, decreasing downstream to

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IntroductionElevated levels of behavioral inhibition (BI) may connote risk for both anxiety and substance use disorders. BI has consistently been shown to be associated with increased levels of anxiety, while the association between BI and substance use has been mixed. It is possible that the relationship between BI and substance use varies by individual difference factors. Hispanic/Latinx (H/L) youth in particular may have stronger relationships between BI, anxiety, and substance use.MethodsThe present study therefore evaluated (1) the prospective relationships between BI [assessed via self-reported behavioral inhibition system (BIS) scale scores], anxiety, and substance use in youth (n = 11,876) across baseline, 1-, and 2-year follow-ups of the Adolescent Brain Cognitive Development (ABCD) Study (ages 9-12) and (2) whether these relationships differed by H/L ethnicity while covarying for average behavioral approach system scores, race, sex, age, highest parental income, highest parental education, and past-year substance use (for analyses involving substance use outcomes).ResultsBaseline levels of BIS scores predicted increased anxiety symptoms at both 1- and 2-year follow-ups and did not differ by H/L ethnicity. Baseline levels of BIS scores also prospectively predicted increased likelihood of substance use at 2-year follow-up, but only for H/L youth and not at 1-year follow-up.DiscussionHigh scores on the BIS scale contribute risk to anxiety across ethnicities and may uniquely contribute to risk for substance use in H/L youth.

Many studies link the compositions of microbial communities to their environments, but the energetics of organism-specific biomass synthesis as a function of geochemical variables have rarely been assessed. We describe a thermodynamic model that integrates geochemical and metagenomic data for biofilms sampled at five sites along a thermal and chemical gradient in the outflow channel of the hot spring known as "Bison Pool" in Yellowstone National Park. The relative abundances of major phyla in individual communities sampled along the outflow channel are modeled by computing metastable equilibrium among model proteins with amino acid compositions derived from metagenomic sequences. Geochemical conditions are represented by temperature and activities of basis species, including pH and oxidation-reduction potential quantified as the activity of dissolved hydrogen. By adjusting the activity of hydrogen, the model can be tuned to closely approximate the relative abundances of the phyla observed in the community profiles generated from BLAST assignments. The findings reveal an inverse relationship between the energy demand to form the proteins at equal thermodynamic activities and the abundance of phyla in the community. The distance from metastable equilibrium of the communities, assessed using an equation derived from energetic considerations that is also consistent with the information-theoretic entropy change, decreases along the outflow channel. Specific divergences from metastable equilibrium, such as an underprediction of the relative abundances of phototrophic organisms at lower temperatures, can be explained by considering additional sources of energy and/or differences in growth efficiency. Although the metabolisms used by many members of these communities are driven by chemical disequilibria, the results support the possibility that higher-level patterns of chemotrophic microbial ecosystems are shaped by metastable equilibrium states that depend on both the composition of biomass and the environmental conditions.

Over 100 hot spring sediment samples were collected from 28 sites in 12 areas/regions, while recording as many coincident geochemical properties as feasible (>60 analytes). PCR was used to screen samples for Korarchaeota 16S rRNA genes. Over 500 Korarchaeota 16S rRNA genes were screened by RFLP analysis and 90 were sequenced, resulting in identification of novel Korarchaeota phylotypes and exclusive geographical variants. Korarchaeota diversity was low, as in other terrestrial geothermal systems, suggesting a marine origin for Korarchaeota with subsequent niche-invasion into terrestrial systems. Korarchaeota endemism is consistent with endemism of other terrestrial thermophiles and supports the existence of dispersal barriers. Korarchaeota were found predominantly in >55°C springs at pH 4.7-8.5 at concentrations up to 6.6×10(6) 16S rRNA gene copies g(-1) wet sediment. In Yellowstone National Park (YNP), Korarchaeota were most abundant in springs with a pH range of 5.7 to 7.0. High sulfate concentrations suggest these fluids are influenced by contributions from hydrothermal vapors that may be neutralized to some extent by mixing with water from deep geothermal sources or meteoric water. In the Great Basin (GB), Korarchaeota were most abundant at spring sources of pH

We have constructed a conceptual model of biogeochemical cycles and metabolic and microbial community shifts within a hot spring ecosystem via coordinated analysis of the "Bison Pool" (BP) Environmental Genome and a complementary contextual geochemical dataset of ~75 geochemical parameters. 2,321 16S rRNA clones and 470 megabases of environmental sequence data were produced from biofilms at five sites along the outflow of BP, an alkaline hot spring in Sentinel Meadow (Lower Geyser Basin) of Yellowstone National Park. This channel acts as a >22 m gradient of decreasing temperature, increasing dissolved oxygen, and changing availability of biologically important chemical species, such as those containing nitrogen and sulfur. Microbial life at BP transitions from a 92 °C chemotrophic streamer biofilm community in the BP source pool to a 56 °C phototrophic mat community. We improved automated annotation of the BP environmental genomes using BLAST-based Markov clustering. We have also assigned environmental genome sequences to individual microbial community members by complementing traditional homology-based assignment with nucleotide word-usage algorithms, allowing more than 70% of all reads to be assigned to source organisms. This assignment yields high genome coverage in dominant community members, facilitating reconstruction of nearly complete metabolic profiles and in-depth analysis of the relation between geochemical and metabolic changes along the outflow. We show that changes in environmental conditions and energy availability are associated with dramatic shifts in microbial communities and metabolic function. We have also identified an organism constituting a novel phylum in a metabolic "transition" community, located physically between the chemotroph- and phototroph-dominated sites. The complementary analysis of biogeochemical and environmental genomic data from BP has allowed us to build ecosystem-based conceptual models for this hot spring, reconstructing whole metabolic networks in order to illuminate community roles in shaping and responding to geochemical variability.

Uncovering the chemical and physical links between natural environments and microbial communities is becoming increasingly amenable owing to geochemical observations and metagenomic sequencing. At the hot spring known as Bison Pool in Yellowstone National Park, the cooling of the water in the outflow channel is associated with an increase in oxidation potential estimated from multiple field-based measurements. Representative groups of proteins whose sequences were derived from metagenomic data also exhibit an increase in average oxidation state of carbon in the protein molecules with distance from the hot-spring source. The energetic requirements of reactions to form selected proteins used in the model were computed using amino-acid group additivity for the standard molal thermodynamic properties of the proteins, and the relative chemical stabilities of the proteins were investigated by varying temperature, pH and oxidation state, expressed as activity of dissolved hydrogen. The relative stabilities of the proteins were found to track the locations of the sampling sites when the calculations included a function for hydrogen activity that increases with temperature and is higher, or more reducing, than values consistent with measurements of dissolved oxygen, sulfide and oxidation-reduction potential in the field. These findings imply that spatial patterns in the amino acid compositions of proteins can be linked, through energetics of overall chemical reactions representing the formation of the proteins, to the environmental conditions at this hot spring, even if microbial cells maintain considerably different internal conditions. Further applications of the thermodynamic calculations are possible for other natural microbial ecosystems.

Type A Clostridium perfringens causes poultry necrotic enteritis (NE), an enteric disease of considerable economic importance, yet can also exist as a member of the normal intestinal microbiota. A recently discovered pore-forming toxin, NetB, is associated with pathogenesis in most, but not all, NE isolates. This finding suggested that NE-causing strains may possess other virulence gene(s) not present in commensal type A isolates. We used high-throughput sequencing (HTS) technologies to generate draft genome sequences of seven unrelated C. perfringens poultry NE isolates and one isolate from a healthy bird, and identified additional novel NE-associated genes by comparison with nine publicly available reference genomes. Thirty-one open reading frames (ORFs) were unique to all NE strains and formed the basis for three highly conserved NE-associated loci that we designated NELoc-1 (42 kb), NELoc-2 (11.2 kb) and NELoc-3 (5.6 kb). The largest locus, NELoc-1, consisted of netB and 36 additional genes, including those predicted to encode two leukocidins, an internalin-like protein and a ricin-domain protein. Pulsed-field gel electrophoresis (PFGE) and Southern blotting revealed that the NE strains each carried 2 to 5 large plasmids, and that NELoc-1 and -3 were localized on distinct plasmids of sizes approximately 85 and approximately 70 kb, respectively. Sequencing of the regions flanking these loci revealed similarity to previously characterized conjugative plasmids of C. perfringens. These results provide significant insight into the pathogenetic basis of poultry NE and are the first to demonstrate that netB resides in a large, plasmid-encoded locus. Our findings strongly suggest that poultry NE is caused by several novel virulence factors, whose genes are clustered on discrete pathogenicity loci, some of which are plasmid-borne.