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Your search keyword '"Everett, Helen E."' showing total 28 results
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28 results on '"Everett, Helen E."'

1. Interspecies Transmission of Swine Influenza A Viruses and Human Seasonal Vaccine-Mediated Protection Investigated in Ferret Model

Author

van Diemen, Pauline M., Byrne, Alexander M.P., Ramsay, Andrew M., Watson, Samantha, Nunez, Alejandro, Moreno, Ana v., Chiapponi, Chiara, Foni, Emanuela, Brown, Ian H., Brookes, Sharon M., and Everett, Helen E.

Subjects
Vaccination -- Health aspects, Medical research -- Health aspects, Medicine, Experimental -- Health aspects, Swine influenza -- Health aspects, Influenza vaccines -- Health aspects, Infection -- Health aspects, Disease transmission -- Health aspects, Health
Abstract

Influenza A viruses (IAVs) have pandemic potential and remain a threat to human and animal health, mainly owing to their intrinsic ability to continually diversify and infect a broad range [...]

Published
2023
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3. Interspecies Transmission of Reassortant Swine Influenza A Virus Containing Genes from Swine Influenza A(H1N1)pdm09 and A(H1N2) Viruses

Author

Everett, Helen E., Nash, Bethany, Londt, Brandon Z., Kelly, Michael D., Coward, Vivien, Nunez, Alejandro, van Diemen, Pauline M., Brown, Ian H., and Brookes, Sharon M.

Subjects
Disease transmission -- Analysis -- Health aspects, Swine influenza -- Analysis -- Health aspects, Genes -- Analysis -- Health aspects, Medical research -- Analysis -- Health aspects, Infection -- Analysis -- Health aspects, Influenza, Lectins, Diseases, Health
Abstract

The ability of swine to support replication of phylogenetically diverse influenza A virus (IAV) strains from avian and mammalian origin poses a public health risk because of the potential for [...]

Published
2020
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7. Respiratory and Intramuscular Immunization With ChAdOx2-NPM1-NA Induces Distinct Immune Responses in H1N1pdm09 Pre-Exposed Pigs

Author

Vatzia, Eleni, primary, Allen, Elizabeth R., additional, Manjegowda, Tanuja, additional, Morris, Susan, additional, McNee, Adam, additional, Martini, Veronica, additional, Kaliath, Reshma, additional, Ulaszewska, Marta, additional, Boyd, Amy, additional, Paudyal, Basudev, additional, Carr, Veronica B., additional, Chrun, Tiphany, additional, Maze, Emmanuel, additional, MacLoughlin, Ronan, additional, van Diemen, Pauline M., additional, Everett, Helen E., additional, Lambe, Teresa, additional, Gilbert, Sarah C., additional, and Tchilian, Elma, additional

Published
2021
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8. Economic evaluation of whole genome sequencing for pathogen identification and surveillance - results of case studies in Europe and the Americas 2016 to 2019

Author

Alleweldt, Frank, Kara, Şenda, Best, Kris, Aarestrup, Frank Møller, Beer, Martin, Bestebroer, Theo M., Campos, Josefina, Casadei, Gabriele, Chinen, Isabel, Van Domselaar, Gary, Dominguez, Catherine, Everett, Helen E., Fouchier, Ron Am, Grant, Kathie, Green, Jonathan, Höper, Dirk, Johnston, Jonathan, Koopmans, Marion Pg, Oude Munnink, Bas B., Myers, Robert, Nadon, Celine, Patel, Ami, Pohlmann, Anne, Pongolini, Stefano, Reimer, Aleisha, Thiessen, Shane, Wylezich, Claudia, Alleweldt, Frank, Kara, Şenda, Best, Kris, Aarestrup, Frank Møller, Beer, Martin, Bestebroer, Theo M., Campos, Josefina, Casadei, Gabriele, Chinen, Isabel, Van Domselaar, Gary, Dominguez, Catherine, Everett, Helen E., Fouchier, Ron Am, Grant, Kathie, Green, Jonathan, Höper, Dirk, Johnston, Jonathan, Koopmans, Marion Pg, Oude Munnink, Bas B., Myers, Robert, Nadon, Celine, Patel, Ami, Pohlmann, Anne, Pongolini, Stefano, Reimer, Aleisha, Thiessen, Shane, and Wylezich, Claudia

Abstract

BackgroundWhole genome sequencing (WGS) is increasingly used for pathogen identification and surveillance.AimWe evaluated costs and benefits of routine WGS through case studies at eight reference laboratories in Europe and the Americas which conduct pathogen surveillance for avian influenza (two laboratories), human influenza (one laboratory) and food-borne pathogens (five laboratories).MethodsThe evaluation focused on the institutional perspective, i.e. the 'investment case' for implementing WGS compared with conventional methods, based on costs and benefits during a defined reference period, mostly covering at least part of 2017. A break-even analysis estimated the number of cases of illness (for the example of Salmonella surveillance) that would need to be avoided through WGS in order to 'break even' on costs.ResultsOn a per-sample basis, WGS was between 1.2 and 4.3 times more expensive than routine conventional methods. However, WGS brought major benefits for pathogen identification and surveillance, substantially changing laboratory workflows, analytical processes and outbreaks detection and control. Between 0.2% and 1.1% (on average 0.7%) of reported salmonellosis cases would need to be prevented to break even with respect to the additional costs of WGS.ConclusionsEven at cost levels documented here, WGS provides a level of additional information that more than balances the additional costs if used effectively. The substantial cost differences for WGS between reference laboratories were due to economies of scale, degree of automation, sequencing technology used and institutional discounts for equipment and consumables, as well as the extent to which sequencers are used at full capacity.

Published
2021

9. Economic evaluation of whole genome sequencing for pathogen identification and surveillance – results of case studies in Europe and the Americas 2016 to 2019

Author

Alleweldt, Frank, primary, Kara, Şenda, additional, Best, Kris, additional, Aarestrup, Frank M, additional, Beer, Martin, additional, Bestebroer, Theo M, additional, Campos, Josefina, additional, Casadei, Gabriele, additional, Chinen, Isabel, additional, Van Domselaar, Gary, additional, Dominguez, Catherine, additional, Everett, Helen E, additional, Fouchier, Ron AM, additional, Grant, Kathie, additional, Green, Jonathan, additional, Höper, Dirk, additional, Johnston, Jonathan, additional, Koopmans, Marion PG, additional, Oude Munnink, Bas B, additional, Myers, Robert, additional, Nadon, Celine, additional, Patel, Ami, additional, Pohlmann, Anne, additional, Pongolini, Stefano, additional, Reimer, Aleisha, additional, Thiessen, Shane, additional, and Wylezich, Claudia, additional

Published
2021
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10. Intranasal Infection of Ferrets with SARS-CoV-2 as a Model for Asymptomatic Human Infection

Author

Everett, Helen E., primary, Lean, Fabian Z. X., additional, Byrne, Alexander M. P., additional, van Diemen, Pauline M., additional, Rhodes, Shelley, additional, James, Joe, additional, Mollett, Benjamin, additional, Coward, Vivien J., additional, Skinner, Paul, additional, Warren, Caroline J., additional, Bewley, Kevin R., additional, Watson, Samantha, additional, Hurley, Shellene, additional, Ryan, Kathryn A., additional, Hall, Yper, additional, Simmons, Hugh, additional, Núñez, Alejandro, additional, Carroll, Miles W., additional, Brown, Ian H., additional, and Brookes, Sharon M., additional

Published
2021
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12. Transmission dynamics between infected waterfowl and terrestrial poultry: Differences between the transmission and tropism of H5N8 highly pathogenic avian influenza virus (clade 2.3.4.4a) among ducks, chickens and turkeys

Author

Puranik, Anita, primary, Slomka, Marek J., additional, Warren, Caroline J., additional, Thomas, Saumya S., additional, Mahmood, Sahar, additional, Byrne, Alexander M.P., additional, Ramsay, Andrew M., additional, Skinner, Paul, additional, Watson, Samantha, additional, Everett, Helen E., additional, Núñez, Alejandro, additional, Brown, Ian H., additional, and Brookes, Sharon M., additional

Published
2020
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13. Statistical modelling of data showing pandemic H1N1 2009 swine influenza A virus infection kinetics in vaccinated pigs

Author

Canini, Laetitia, primary, Everett, Helen E., additional, Aramouni, Mario, additional, Coward, Vivien, additional, Ramsay, Andrew, additional, Kelly, Michael, additional, Morgan, Sophie, additional, Tchilian, Elma, additional, Gilbert, Sarah, additional, Charleston, Bryan, additional, Brown, Ian H., additional, Brookes, Sharon M., additional, and Woolhouse, Mark E.J., additional

Published
2019
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15. Vaccine-mediated protection of pigs against infection with pandemic H1N1 2009 swine influenza A virus requires a close antigenic match between the vaccine antigen and challenge virus

Author

Everett, Helen E., primary, Aramouni, Mario, additional, Coward, Vivien, additional, Ramsay, Andrew, additional, Kelly, Michael, additional, Morgan, Sophie, additional, Tchilian, Elma, additional, Canini, Laetitia, additional, Woolhouse, Mark E.J., additional, Gilbert, Sarah, additional, Charleston, Bryan, additional, Brown, Ian H., additional, and Brookes, Sharon M., additional

Published
2019
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16. Vaccines That Reduce Viral Shedding Do Not Prevent Transmission of H1N1 Pandemic 2009 Swine Influenza A Virus Infection to Unvaccinated Pigs.

Author

Everett, Helen E., van Diemen, Pauline M., Aramouni, Mario, Ramsay, Andrew, Coward, Vivien J., Pavot, Vincent, Canini, Laetitia, Holzer, Barbara, Morgan, Sophie, Woolhouse, Mark E. J., Tchilian, Elma, Brookes, Sharon M., Brown, Ian H., Charleston, Bryan, and Gilbert, Sarah

Subjects
*PANDEMICS, *H1N1 influenza, *VIRUS diseases, *VIRAL shedding, *SWINE influenza, *SWINE, *INFLUENZA A virus
Abstract

Swine influenza A virus (swIAV) infection causes substantial economic loss and disease burden in humans and animals. The 2009 pandemic H1N1 (pH1N1) influenza A virus is now endemic in both populations. In this study, we evaluated the efficacy of different vaccines in reducing nasal shedding in pigs following pH1N1 virus challenge. We also assessed transmission from immunized and challenged pigs to naive, directly in-contact pigs. Pigs were immunized with either adjuvanted, whole inactivated virus (WIV) vaccines or virus-vectored (ChAdOx1 and MVA) vaccines expressing either the homologous or heterologous influenza A virus hemagglutinin (HA) glycoprotein, as well as an influenza virus pseudotype (S-FLU) vaccine expressing heterologous HA. Only two vaccines containing homologous HA, which also induced high hemagglutination inhibitory antibody titers, significantly reduced virus shedding in challenged animals. Nevertheless, virus transmission from challenged to naive, in-contact animals occurred in all groups, although it was delayed in groups of vaccinated animals with reduced virus shedding. [ABSTRACT FROM AUTHOR]

Published
2021
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17. Inactivated pandemic 2009 H1N1 influenza A virus human vaccines have different efficacy after homologous challenge in the ferret model.

Author

Vidaña, Beatriz, Brookes, Sharon M., Everett, Helen E., Garcon, Fanny, Nuñez, Alejandro, Engelhardt, Othmar, Major, Diane, Hoschler, Katja, Brown, Ian H., and Zambon, Maria

Subjects
H1N1 influenza, PANDEMICS, INFLUENZA vaccines, RESPIRATORY infections, FERRET, VIRAL antibodies, VIRAL shedding
Abstract

Background: The 2009 pandemic H1N1 (A(H1N1)pdm09) influenza A virus (IAV) has replaced the previous seasonal H1N1 strain in humans and continues to circulate worldwide. The comparative performance of inactivated A(H1N1)pdm09 influenza vaccines remains of considerable interest. The objective of this study was to evaluate the efficacy of two licensed A(H1N1)pdm09 inactivated vaccines (AS03B adjuvanted split virion Pandemrix from GlaxoSmithKline and referred here as (V1) and non‐adjuvanted whole virion Celvapan from Baxter and referred here as (V2)) in ferrets as a pre‐clinical model for human disease intervention. Methods: Naïve ferrets were divided into two groups (V1 and V2) and immunised intramuscularly with two different A/California/07/2009‐derived inactivated vaccines, V1 administered in a single dose and V2 administered in 2 doses separated by 21 days. Six weeks after the first immunisation, vaccinated animals and a non‐vaccinated control (NVC) group were intra‐nasally challenged with 106.5 TCID50 of the isolate A/England/195/2009 A(H1N1)pdm09 with 99.1% amino acid identity to the vaccine strain. Clinical signs, lung histopathology, viral quantification and antibody responses were evaluated. Results and Conclusions: Results revealed important qualitative differences in the performance of both inactivated vaccines in relation to protection against challenge with a comparable virus in a naive animal (ferret) model of human disease. Vaccine V1 limited and controlled viral shedding and reduced lower respiratory tract infection. In contrast, vaccine V2 did not control infection and animals showed sustained viral shedding and delayed lower respiratory infection, resulting in pulmonary lesions, suggesting lower efficacy of V2 vaccine. [ABSTRACT FROM AUTHOR]

Published
2021
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18. CD1− and CD1+ porcine blood dendritic cells are enriched for the orthologues of the two major mammalian conventional subsets

Author

Edwards, Jane C., primary, Everett, Helen E., additional, Pedrera, Miriam, additional, Mokhtar, Helen, additional, Marchi, Emanuele, additional, Soldevila, Ferran, additional, Kaveh, Daryan A., additional, Hogarth, Philip J., additional, Johns, Helen L., additional, Nunez-Garcia, Javier, additional, Steinbach, Falko, additional, Crooke, Helen R., additional, and Graham, Simon P., additional

Published
2017
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19. Aerosol Delivery of a Candidate Universal Influenza Vaccine Reduces Viral Load in Pigs Challenged with Pandemic H1N1 Virus

Author

Morgan, Sophie B., primary, Hemmink, Johanneke D., additional, Porter, Emily, additional, Harley, Ross, additional, Shelton, Holly, additional, Aramouni, Mario, additional, Everett, Helen E., additional, Brookes, Sharon M., additional, Bailey, Michael, additional, Townsend, Alain M., additional, Charleston, Bryan, additional, and Tchilian, Elma, additional

Published
2016
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21. Partial Activation of Natural Killer and γδ T Cells by Classical Swine Fever Viruses Is Associated with Type I Interferon Elicited from Plasmacytoid Dendritic Cells

Author

Franzoni, Giulia, primary, Edwards, Jane C., additional, Kurkure, Nitin V., additional, Edgar, Daniel S., additional, Sanchez-Cordon, Pedro J., additional, Haines, Felicity J., additional, Salguero, Francisco J., additional, Everett, Helen E., additional, Bodman-Smith, Kikki B., additional, Crooke, Helen R., additional, and Graham, Simon P., additional

Published
2014
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25. Challenge of Pigs with Classical Swine Fever Viruses after C-Strain Vaccination Reveals Remarkably Rapid Protection and Insights into Early Immunity

Author

Graham, Simon P., primary, Everett, Helen E., additional, Haines, Felicity J., additional, Johns, Helen L., additional, Sosan, Olubukola A., additional, Salguero, Francisco J., additional, Clifford, Derek J., additional, Steinbach, Falko, additional, Drew, Trevor W., additional, and Crooke, Helen R., additional

Published
2012
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26. Partial Activation of Natural Killer and ?d T Cells by Classical Swine Fever Viruses Is Associated with Type I Interferon Elicited from Plasmacytoid Dendritic Cells

Author

Franzoni, Giulia, Edwards, Jane C., Kurkure, Nitin V., Edgar, Daniel S., Sanchez-Cordon, Pedro J., Haines, Felicity J., Salguero, Francisco J., Everett, Helen E., Bodman-Smith, Kikki B., Crooke, Helen R., and Graham, Simon P.

Abstract

ABSTRACTVaccination with live attenuated classical swine fever virus (CSFV) vaccines can rapidly confer protection in the absence of neutralizing antibodies. With an aim of providing information on the cellular mechanisms that may mediate this protection, we explored the interaction of porcine natural killer (NK) cells and ?d T cells with CSFV. Both NK and ?d T cells were refractory to infection with attenuated or virulent CSFV, and no stimulatory effects, as assessed by the expression of major histocompatibility complex (MHC) class II (MHC-II), perforin, and gamma interferon (IFN-?), were observed when the cells were cultured in the presence of CSFV. Coculture with CSFV and myeloid dendritic cells (mDCs) or plasmacytoid dendritic cells (pDCs) showed that pDCs led to a partial activation of both NK and ?d T cells, with upregulation of MHC-II being observed. An analysis of cytokine expression by infected DC subsets suggested that this effect was due to IFN-a secreted by infected pDCs. These results were supported by ex vivoanalyses of NK and ?d T cells in the tonsils and retropharyngeal lymph nodes from pigs that had been vaccinated with live attenuated CSFV and/or virulent CSFV. At 5 days postchallenge, there was evidence of significant upregulation of MHC-II but not perforin on NK and ?d T cells, which was observed only following a challenge of the unvaccinated pigs and correlated with increased CSFV replication and IFN-a expression in both the tonsils and serum. Together, these data suggest that it is unlikely that NK or ?d T cells contribute to the cellular effector mechanisms induced by live attenuated CSFV.

Published
2014
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27. CD1− and CD1+ porcine blood dendritic cells are enriched for the orthologues of the two major mammalian conventional subsets.

Author

Edwards, Jane C., Everett, Helen E., Pedrera, Miriam, Mokhtar, Helen, Marchi, Emanuele, Soldevila, Ferran, Kaveh, Daryan A., Hogarth, Philip J., Johns, Helen L., Nunez-Garcia, Javier, Steinbach, Falko, Crooke, Helen R., and Graham, Simon P.

Abstract

Conventional dendritic cells (cDC) are professional antigen-presenting cells that induce immune activation or tolerance. Two functionally specialised populations, termed cDC1 and cDC2, have been described in humans, mice, ruminants and recently in pigs. Pigs are an important biomedical model species and a key source of animal protein; therefore further understanding of their immune system will help underpin the development of disease prevention strategies. To characterise cDC populations in porcine blood, DC were enriched from PBMC by CD14 depletion and CD172a enrichment then stained with lineage mAbs (Lin; CD3, CD8α, CD14 and CD21) and mAbs specific for CD172a, CD1 and CD4. Two distinct porcine cDC subpopulations were FACSorted CD1− cDC (Lin−CD172+ CD1−CD4−) and CD1+ cDC (Lin−CD172a+ CD1+ CD4−), and characterised by phenotypic and functional analyses. CD1+ cDC were distinct from CD1− cDC, expressing higher levels of CD172a, MHC class II and CD11b. Following TLR stimulation, CD1+ cDC produced IL-8 and IL-10 while CD1− cDC secreted IFN-α, IL-12 and TNF-α. CD1− cDC were superior in stimulating allogeneic T cell responses and in cross-presenting viral antigens to CD8 T cells. Comparison of transcriptional profiles further suggested that the CD1− and CD1+ populations were enriched for the orthologues of cDC1 and cDC2 subsets respectively. [ABSTRACT FROM AUTHOR]

Published
2017
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28. CD1 - and CD1 + porcine blood dendritic cells are enriched for the orthologues of the two major mammalian conventional subsets.

Author

Edwards JC, Everett HE, Pedrera M, Mokhtar H, Marchi E, Soldevila F, Kaveh DA, Hogarth PJ, Johns HL, Nunez-Garcia J, Steinbach F, Crooke HR, and Graham SP

Subjects
Animals, Antigens, Surface analysis, Blood Cells classification, Cytokines metabolism, Dendritic Cells classification, Flow Cytometry, Gene Expression Profiling, Swine, Swine Diseases, Antigens, CD1 analysis, Blood Cells chemistry, Blood Cells immunology, Dendritic Cells chemistry, Dendritic Cells immunology
Abstract

Conventional dendritic cells (cDC) are professional antigen-presenting cells that induce immune activation or tolerance. Two functionally specialised populations, termed cDC1 and cDC2, have been described in humans, mice, ruminants and recently in pigs. Pigs are an important biomedical model species and a key source of animal protein; therefore further understanding of their immune system will help underpin the development of disease prevention strategies. To characterise cDC populations in porcine blood, DC were enriched from PBMC by CD14 depletion and CD172a enrichment then stained with lineage mAbs (Lin; CD3, CD8α, CD14 and CD21) and mAbs specific for CD172a, CD1 and CD4. Two distinct porcine cDC subpopulations were FACSorted CD1 - cDC (Lin - CD172 + CD1 - CD4 - ) and CD1 + cDC (Lin - CD172a + CD1 + CD4 - ), and characterised by phenotypic and functional analyses. CD1 + cDC were distinct from CD1 - cDC, expressing higher levels of CD172a, MHC class II and CD11b. Following TLR stimulation, CD1 + cDC produced IL-8 and IL-10 while CD1 - cDC secreted IFN-α, IL-12 and TNF-α. CD1 - cDC were superior in stimulating allogeneic T cell responses and in cross-presenting viral antigens to CD8 T cells. Comparison of transcriptional profiles further suggested that the CD1 - and CD1 + populations were enriched for the orthologues of cDC1 and cDC2 subsets respectively.

Published
2017
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