1. Detection of hepatitis E virus genotype 3 in pigs from subsistence farms in the state of Mato Grosso, Brazil
- Author
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Evandro Almeida Teixeira, Valéria Dutra, Cláudio Wageck Canal, Simone Silveira, D. M. Schenkel, Marcos de Almeida Souza, Caroline Argenta Pescador, Lucinano Nakazato, Camila Gonçalves de Campos, and Henrique Carvalho
- Subjects
0301 basic medicine ,Veterinary medicine ,Farms ,Meat ,Genotype ,Swine ,viruses ,Immunology ,Biology ,medicine.disease_cause ,Microbiology ,law.invention ,Feces ,03 medical and health sciences ,Hepatitis E virus ,law ,Zoonoses ,medicine ,Animals ,Immunology and Allergy ,Phylogeny ,Swine Diseases ,General Veterinary ,Zoonosis ,Hepatitis E virus genotype ,Genetic Variation ,Subsistence agriculture ,General Medicine ,Hepatitis E ,medicine.disease ,Serum samples ,030104 developmental biology ,Infectious Diseases ,Transmission (mechanics) ,RNA, Viral ,Brazil - Abstract
Hepatitis E is a zoonotic disease, recognized as an important global public health concern. In this study, molecular detection of the ORF1 and ORF2 genomic regions of the hepatitis E virus (HEV) was carried out in fecal and serum samples from pigs in subsistence farms of Mato Grosso, Brazil. Fragments of the ORF2 region were amplified in 8% (12/150) of fecal samples, with 53.3% (8/15) of farms having positive results. Of the 12 positive samples, fragments of the ORF1 region were amplified in 33.3% (4/12) of these. Molecular characterization confirmed the phylogenetic groupings as HEV subtypes 3d, 3 h, and 3i. The results revealed that meat from pigs that was originally meant for personal consumption is being traded in marketplaces in metropolitan Cuiaba, thereby creating a source of transmission to consumers in Mato Grosso. The environmental conditions must be taken into account when investigating the presence and transmission of HEV.
- Published
- 2018
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