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A high-throughput and highly efficient analytical platform for urine drug screening is critical in both clinical and forensic settings. Mass spectrometry (MS) has better sensitivity and specificity than conventional immunoassays (IA); however, not all laboratories have the necessary resources and workforce to operate MS. The goal of this study was to evaluate a multidrug biochip with 20 discrete testing regions (DTRs) for high-throughput urine drug screening (UDS).The Randox DOA Ultra Urine (DOAULT URN) biochip employs chemiluminescent IA to detect various analytes, including stimulants, hallucinogens, sedatives, narcotics, and dextromethorphan. The verification included the evaluation of the limits of detection (LOD), stability of calibrators and controls, cross-reactivity, carryover, interference, and overall performance.LODsquality control low for each DTR. The reconstituted calibrators were stable for up to 2 weeks at -20°C. Controls were stable for 4-6 hours at 22-25°C, with20% within-day and ≤23% between-day imprecision. The accuracy of the controls (%bias) was within ±20% of the target concentration, except for dextromethorphan at -23.8%. No interference was observed with common over-the-counter medications. No carryover was detected in the high-concentration samples. Satisfactory cross-reactivity (≥50%) with known analytes produced presumptive positive results, with readings higher than the proposed decision points. The overall biochip performance of 165 confirmed samples showed 98.0% sensitivity, 96.9% specificity, and 97.5% efficiency.The DOAULT URN biochip is a multidrug analyte IA capable of detecting dozens of parent drugs and their metabolites in urine. It offers clinical and forensic laboratories an alternative UDS tool with LODs comparable to those of MS.

¿Existe suficiente documentación que muestre realmente el rol que el violín ha desempeñado en el flamenco a lo largo de su historia? ¿Qué relación ha mantenido este instrumento con dicho arte? ¿Se conocen aspectos relativos a la interpretación del flamenco a través del violín? El presente artículo pretende llegar a conocer la presencia del violín en el flamenco a lo largo de su andadura como manifestación artística. Para ello, se vuelve especialmente relevante abordar una profunda investigación que localice toda la documentación disponible al respecto y que, por tanto, consiga aportar un corpus de aquellas indagaciones que, tras un estudio concienzudo, se han preocupado por esclarecer el vínculo existente entre ambos campos.