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Your search keyword '"European Project: ANR-11-LABX-0003-01"' showing total 2 results
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2 results on '"European Project: ANR-11-LABX-0003-01"'

1. Kinetics of isothermal dumbbell exponential amplification: effects of mix composition on LAMP and its derivatives

Author

Maud Savonnet, Mathilde Aubret, Patricia Laurent, Yoann Roupioz, Myriam Cubizolles, Arnaud Buhot, Chimie pour la Reconnaissance et l’Etude d’Assemblages Biologiques (CREAB ), SYstèmes Moléculaires et nanoMatériaux pour l’Energie et la Santé (SYMMES), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA), Département Microtechnologies pour la Biologie et la Santé (DTBS), Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information (CEA-LETI), Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), ANR-17-EURE-0003,CBH-EUR-GS,CBH-EUR-GS(2017), ANR-11-LABX-0003,ARCANE,Grenoble, une chimie bio-motivée(2011), and European Project: ANR-11-LABX-0003-01

Subjects
Kinetics, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, Molecular Diagnostic Techniques, loop mediated isothermal amplification (LAMP), isothermal dumbbell exponential amplification (IDEA), kinetic model, nucleic acids amplification, logistic function, [CHIM.ORGA]Chemical Sciences/Organic chemistry, Clinical Biochemistry, DNA, General Medicine, Nucleic Acid Amplification Techniques
Abstract

International audience; Loop-mediated isothermal amplification (LAMP) is an exponential amplification method of DNA strands that is more and more used for its high performances. Thanks to its high sensitivity and selectivity, LAMP found numerous applications from the detection of pathogens or viruses through their genome amplification to its incorporation as an amplification strategy in protein or miRNA biomarker quantification. The LAMP method is composed of two stages: the first one consists in the transformation of the DNA strands into dumbbell structures formed of two stems and loops thanks to four primers; then, in the second stage, only two primers are required to amplify the dumbbells exponentially in numerous hairpins of increasing lengths. In this paper, we propose a theoretical framework to analyze the kinetics of the second stage of LAMP, the isothermal dumbbell exponential amplification (IDEA) as function of the physico-chemical parameters of the amplification reaction. Dedicated experiments validate the models. We believe these results may help the optimization of LAMP performances by reducing the number of experiments necessary to find the best parameters.

Published
2022
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2. A membraneless air-breathing hydrogen biofuel cell based on direct wiring of thermostable enzymes on carbon nanotube electrodes

Author

Pascale Infossi, Michael Holzinger, Sébastien Gounel, Raoudha Haddad, Anne de Poulpiquet, Michel Mermoux, Elisabeth Lojou, Noémie Lalaoui, Serge Cosnier, Nicolas Mano, Alan Le Goff, Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA), Département de Chimie Moléculaire (DCM), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Joseph Fourier - Grenoble 1 (UJF), Bioénergétique et Ingénierie des Protéines (BIP ), Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Joseph Fourier - Grenoble 1 (UJF), Centre de Recherche Paul Pascal (CRPP), Université de Bordeaux (UB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Electrochimie et de Physico-chimie des Matériaux et des Interfaces (LEPMI ), Institut de Chimie du CNRS (INC)-Institut National Polytechnique de Grenoble (INPG)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Université Joseph Fourier - Grenoble 1 (UJF)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS), ANR-13-BIME-0003,CAROUCELL,Nanostructuration des anode et cathode pour une biopile à combustible H2/O2(2013), European Project: ANR-11-LABX-0003-01, European Project: ANR-12-BS08-0011-01, Université Joseph Fourier - Grenoble 1 (UJF)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Université Joseph Fourier - Grenoble 1 (UJF)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut National Polytechnique de Grenoble (INPG)-Institut de Chimie du CNRS (INC)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS)

Subjects
Oxidoreductases Acting on CH-CH Group Donors, Materials science, Hydrogenase, Hydrogen, Bioelectric Energy Sources, Carbon nanotubes, chemistry.chemical_element, Bacillus, Nanotechnology, 02 engineering and technology, Carbon nanotube, 010402 general chemistry, 7. Clean energy, 01 natural sciences, Catalysis, law.invention, law, Materials Chemistry, Bilirubin oxidase, Electrodes, biology, Nanotubes, Carbon, Bacillus pumilus, Air, fungi, Metals and Alloys, Biofuel cell, [CHIM.CATA]Chemical Sciences/Catalysis, General Chemistry, Enzymes, Immobilized, 021001 nanoscience & nanotechnology, biology.organism_classification, Cathode, Enzymes, 0104 chemical sciences, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials, Anode, Oxygen, [CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry, Chemical engineering, chemistry, Electrode, Ceramics and Composites, 0210 nano-technology, Oxidation-Reduction
Abstract

International audience; A biocathode was designed by the modification of a carbon nanotube (CNT) gas-diffusion electrode with bilirubin oxidase from Bacillus pumilus, achieving high current densities up to 3 mA cm−2 for the reduction of O2 from air. A membraneless air-breathing hydrogen biofuel cell was designed by combination of this cathode with a functionalized CNT-based hydrogenase anode.

Published
2015
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