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6 results on '"Etges, Rodrigo N."'

1. Genomic analysis of an outbreak of bovine tuberculosis in a man‐made multi‐host species system: A call for action on wildlife in Brazil

Author

Lima, Daiane A. R., primary, Zimpel, Cristina K., additional, Patané, José S., additional, Silva‐Pereira, Taiana Tainá, additional, Etges, Rodrigo N., additional, Rodrigues, Rudielle A., additional, Dávila, Alberto M.R., additional, Ikuta, Cássia Y., additional, Ferreira Neto, José S., additional, Guimarães, Ana Marcia S., additional, and Araújo, Flábio R., additional

Published
2021
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2. Genomic analysis of an outbreak of bovine tuberculosis in a man‐made multi‐host species system: A call for action on wildlife in Brazil.

Author

Lima, Daiane A. R., Zimpel, Cristina K., Patané, José S., Silva‐Pereira, Taiana Tainá, Etges, Rodrigo N., Rodrigues, Rudielle A., Dávila, Alberto M.R., Ikuta, Cássia Y., Ferreira Neto, José S., Guimarães, Ana Marcia S., and Araújo, Flábio R.

Subjects
TUBERCULOSIS in cattle, GENOMICS, MYCOBACTERIUM bovis, SPECIES, TUBERCULIN test, NUCLEOTIDE sequencing
Abstract

We report on a 15‐year‐long outbreak of bovine tuberculosis (bTB) in wildlife from a Brazilian safari park. A timeline of diagnostic events and whole‐genome sequencing (WGS) of 21 Mycobacterium bovis isolates from deer and llamas were analyzed. Accordingly, from 2003 to 2018, at least 16 animals, from eight species, died due to TB, which is likely an underestimated number. In three occasions since 2013, the deer presented positive tuberculin tests, leading to the park closure and culling of all deer. WGS indicated that multiple M. bovis strains were circulating, with at least three founding introductions since the park inauguration in 1977. Using a previously sequenced dataset of 71 M. bovis genomes from cattle, we found no recent transmission events between nearby farms and the park based on WGS. Lastly, by discussing socio‐economic and environmental factors escaping current regulatory gaps that were determinant of this outbreak, we pledge for the development of a plan to report and control bTB in wildlife in Brazil. [ABSTRACT FROM AUTHOR]

Published
2022
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4. Matrix assisted laser desorption ionization-time-of-flight mass spectrometry identification of mycobacterium bovis in bovinae

Author

Bacanelli, Gisele, Olarte, Larissa C., Silva, Marcio Roberto, Rodrigues, Rudielle A., Carneiro, Paulo A. M., Kannene, John B., Pasquatti, Taynara N., Takatani, Haruo, Zumarraga, Martin Jose, Etges, Rodrigo N., Araujo, Flabio Ribeiro de, and Verbisck, Newton V.

Subjects
Espectrometría, Spectrometry, Bovina, Mycobacterium bovis Infections, Tuberculosis, Infeccion Mycobacterium tuberculosis, Desorption, Infeccion por Mycobacterium bovis, Ionization Treatment, Mycobacterium tuberculosis Infections, Desorción, Bovinae, Tratamiento Ionizante
Abstract

In this study, Matrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDI-TOF) mass spectrometry was used to identify Mycobacterium bovis from cattle and buffalo tissue isolates from the North and South regions of Brazil, grown in solid medium and previously identified by Polymerase Chain Reaction (PCR) based on Region of Difference 4 (RD4), sequencing and spoligotyping. For this purpose, the protein extraction protocol and the mass spectra reference database were optimized for the identification of 80 clinical isolates of mycobacteria. As a result of this optimization, it was possible to identify and differentiate M. bovis from other members of the Mycobacterium tuberculosis complex with 100% specificity, 90.91% sensitivity and 91.25% reliability. MALDI-TOF MS methodology described herein provides successful identification of M. bovis within bovine/bubaline clinical samples, demonstrating its usefulness for bovine tuberculosis diagnosis in the future. Instituto de Biotecnología Fil: Bacanelli, Gisele. Federal University of Mato Grosso do Sul. Biotechnology and Biodiversity of the Central Western Region Postgraduate Program; Brasil Fil: Olarte, Larissa C. Federal University of Mato Grosso do Sul. Biochemistry and Molecular Biology Multicentric Postgraduate Program; Brasil Fil: Silva, Marcio Roberto. Empresa Brasileira de Pesquisa Agropecuária (Embrapa). Gado de Leite; Brasil Fil: Rodrigues, Rudielle A. Federal University of Mato Grosso do Sul. Faculty of Veterinary Medicine. Veterinary Sciences Postgraduate Program; Brasil Fil: Carneiro, Paulo A. M. Michigan State University. Center for Comparative Epidemiology; Estados Unidos Fil: Kannene, John B. Michigan State University. Center for Comparative Epidemiology; Estados Unidos Fil: Pasquatti, Taynara N. Dom Bosco Catholic University; Brasil Fil: Takatani, Haruo. Agricultural Defense Agency of Amazonas; Brasil Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Etges, Rodrigo N. Secretary of Agriculture, Livestock and Irrigation; Brasil Fil: Araujo, Flabio Ribeiro de. Empresa Brasileira de Pesquisa Agropecuária (Embrapa). Gado de Corte; Brasil Fil: Verbisck, Newton V. Empresa Brasileira de Pesquisa Agropecuária (Embrapa). Gado de Corte; Brasil

Published
2019

5. Matrix Assisted Laser Desorption Ionization-Time-of-Flight mass spectrometry identification of Mycobacterium bovis in Bovinae

Author

BACANELLI, Gisele, primary, OLARTE, Larissa C., additional, SILVA, Márcio R., additional, RODRIGUES, Rudielle A., additional, CARNEIRO, Paulo A.M., additional, KANEENE, John B., additional, PASQUATTI, Taynara N., additional, TAKATANI, Haruo, additional, ZUMÁRRAGA, Martin J., additional, ETGES, Rodrigo N., additional, ARAÚJO, Flábio R., additional, and VERBISCK, Newton V., additional

Published
2019
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