13 results on '"Estévez-Priego, Estefanía"'
Search Results
2. HeNeCOn: An Ontology for Integrative Research in Head and Neck Cancer
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Hernández, Liss, primary, Estévez-Priego, Estefanía, additional, López-Pérez, Laura, additional, Fernanda Cabrera-Umpiérrez, María, additional, Arredondo, María T., additional, and Fico, Giuseppe, additional
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- 2023
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3. Long-term calcium imaging reveals functional development in hiPSC-derived cultures comparable to human but not rat primary cultures
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Estévez-Priego, Estefanía, primary, Moreno-Fina, Martina, additional, Monni, Emanuela, additional, Kokaia, Zaal, additional, Soriano, Jordi, additional, and Tornero, Daniel, additional
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- 2023
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4. Light sheet fluorescence microscopy for 3D imaging of Ca2+ dynamics in neuronal cultures
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Loza-Alvarez, Pablo, primary, Castro-Olvera, Gustavo, additional, Madrid-Wolff, Jorge A., additional, Olarte, Omar E., additional, Gualda, Emilio J., additional, Soriano, Jordi, additional, Estévez-Priego, Estefanía, additional, and Ludl, Adriaan A., additional
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- 2021
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5. In Vitro Development of Human iPSC-Derived Functional Neuronal Networks on Laser-Fabricated 3D Scaffolds
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Koroleva, Anastasia, primary, Deiwick, Andrea, additional, El-Tamer, Ayman, additional, Koch, Lothar, additional, Shi, Yichen, additional, Estévez-Priego, Estefanía, additional, Ludl, Adriaan-Alexander, additional, Soriano, Jordi, additional, Guseva, Daria, additional, Ponimaskin, Evgeni, additional, and Chichkov, Boris, additional
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- 2021
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6. Dynamics and Effective Connectivity in Bi- and Three–dimensional Neuronal Cultures: from Self–organization to Engineering
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Estévez Priego, Estefanía, Soriano i Fradera, Jordi, Tornero, Daniel, Universitat de Barcelona. Departament de Física de la Matèria Condensada, and Franzese, Giancarlo
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Células madre ,Cultivo celular ,Neurociencias ,Neurosciences ,Biophysics ,Neurociències ,Cell culture ,Stem cells ,Cèl·lules mare ,538.9 ,Biofísica ,Ciències Experimentals i Matemàtiques ,Cultiu cel·lular - Abstract
[eng] This thesis was part of the European consortium MESOBRAIN, a team of 5 organizations that joined efforts in nanofabrication, cell culturing, imaging and data analysis to build tailored human 3D networks. The thesis timing was limited to 3 years, and several of the resources needed for its development were built from scratch. The main objective of this Ph.D. thesis was to explore complex characteristics of cortical neuronal cultures in terms of effective connectivity and exhaustive network analyses. This objective comprised four research lines: (i) The evaluation of neuronal network resilience and emerging plasticity mechanisms, (ii) the characterization of functional development to underline crucial timepoints in healthy neuronal networks, (iii) the study of 3D network interactions of neurons embedded inside an ECM--like environment, and (iv) the design, construction and viability inspection of neurons seeded on tiny 3D nanoprinted solid scaffold structures as a first step towards recreating cortical columns in vitro. For these multiple lines, we used either primary rat cultures (i,iii,iv) or human--derived neurons (ii). The former group corresponds to cultures with long established protocols that have been thoroughly studied in the field. The latter group corresponds to human neurons derived from iPSCs, a relatively novel model with promising and thrilling applications in regenerative medicine. Despite the increasing use of stem cells in neuroscience, complex systems and medicine, they still lack a thorough exploration in terms of neuronal and circuit formation as well as the properties of the emergent activity patterns. With either primary or stem cells, we explored the formation of neuronal circuits in 2D and 3D, characterized the effective connectivity and rendered a number of network traits. This Thesis combines experiments of highly difficult implementation with detailed data analysis. It was necessary to develop brand new protocols for culturing 3D neuronal networks and for human-derived neurons, the use of different microscopy setups the programming of object detection and tracking software and advance the analysis toolbox of calcium fluorescence data. First, resilience experiments on primary clustered neuronal cultures consisted on progressive perturbations through chemical receptor antagonists. This study represents an inspiring numerical--experimental model to comprehend the impact of plasticity mechanisms in the spontaneous activity of neuronal circuits. The results showed that, upon progressive connectivity blockade through chemical receptors' antagonists, only--excitatory neuronal networks displayed a surprising hyper--efficiency (HE) state for early--onset doses. As plasticity mechanisms influence the response of effective connectivity in the presence of perturbations, these compensatory mechanisms, usually disregarded, must be included in biological modeling as accurately as possible. Otherwise, episodes of functional rewiring and synaptic strengthening could mask important phenomena during experiments that alter channel communication. A simple algorithm that hypothesized an effective synaptic scaling was able to capture the hyper--efficiency state seen in experimental data, while percolation models wrongly predicted a progressive decay. The second research line was a sum of engineering efforts within the MESOBRAIN consortium, the European adventure to build 3D neuronal cultures embedded in hydrogels and with the presence of scaffolds. After several months of biomaterials testing, the candidate D--Clear resulted suitable for the construction of scaffolds, both with primary rat cells and hiPSCs, due to its good optical properties, manageability and biocompatibility. To our knowledge, D--Clear was never used before outside the orthodontic field and could provide a new catalogue of interesting designs for support and guidance of neuronal assemblies. Using this material, we developed a series of designs to offer support and guidance to cortical neurons in a 3D platform. The third research line focused on the study of neuronal development and cell-to-cell interactions in a semi-synthetic hydrogel that resembles the extracellular matrix of the brain. These hydrogel cultures keep the advantages of in vitro models while achieving an effective connectivity and architecture closer to in vivo. Finally, the fourth line of research applied cortical neurons from human-derived pluripotent stem cells to study key developmental stages and characterize the healthy maturation of these cells in vitro. As this technology has tremendous potential for regenerative medicine and to model neuronal diseases, it is urgent to consolidate the capacity of these human neuronal networks to reproduce efficient activity patterns of healthy patients, and explore the differences against the results obtained with animal models., [spa] La presente tesis doctoral se enmarca en el contexto de la Física de la Materia Condensada, la Biofísica y la Neurociencia. Principalmente, se centra en el estudio de la conectividad funcional en cultivos neuronales bidimensionales (2D) y tridimensionales (3D). El trabajo se ha desarrollado en el Laboratorio del director de tesis Dr. Jordi Soriano, en la Facultad de Física de la Universitat de Barcelona, junto con el codirector Dr. Daniel Tornero, en el Hospital Clínic de Barcelona. Esta tesis forma parte del proyecto europeo MESO-BRAIN, del programa Future and Emergent Technologies (FET) de la Comisión Europea, Horizon2020. El trabajo de investigación combina experimentos con cultivos neuronales (de rata embrionaria o células humanas pluripotentes) y un análisis detallado en el contexto de teoría de redes y sistemas complejos. Los principales núcleos del trabajo realizado son los siguientes: (i) Actividad funcional en cultivos de redes neuronales y los mecanismos homeostáticos que emergen en presencia de perturbaciones; (ii) el desarrollo de herramientas de neuroingeniería para preparar cultivos ad hoc con conectividad dirigida mediante scaffolds; (iii) el análisis exhaustivo de los procesos de formación y madurez de redes funcionales humanas obtenidas de células madre pluripotentes inducidas, una nueva tecnología que promete revolucionar el campo de la medicina regenerativa; y (iv) la caracterización de cultivos neuronales 3D en estructuras que imitan la matriz extracelular natural de su entorno. Entre las diversas técnicas para la realización de cultivos tridimensionales, destacan los hidrogeles semi-sintéticos, constituidos en base a polímeros altamente hidratados con alta biocompatibilidad y cuyas propiedades mecánicas pueden ser manipuladas para obtener la estructura óptima según el tipo de tejido. En conjunto, los resultados de la presente tesis muestran la gran versatilidad de los cultivos neuronales y aportan avances relevantes en el estudio de plasticidad en redes neuronales, madurez y desarrollo tanto en 2D como en 3D, con sus correspondientes diferencias, incluyendo el uso de neuronas humanas derivadas de células madre inducidas. En el futuro, estos estudios nos permitirán incrementar nuestro conocimiento sobre el funcionamiento global del cerebro y avanzar en la investigación de diferentes enfermedades neurodegenerativas.
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- 2019
7. Light sheet microscopy for fast functional imaging of 3D neuronal cultures in hydrogels
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Castro-Olvera, Gustavo, primary, Madrid-Wolff, Jorge, additional, Olarte, Omar E., additional, Estévez-Priego, Estefanía, additional, Ludl, Adriaan A., additional, Gualda, Emilio J., additional, Ladepeche, Laurent, additional, Soriano, Jordi, additional, and Loza-Alvarez, Pablo, additional
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- 2020
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8. Functional strengthening through synaptic scaling upon connectivity disruption in neuronal cultures
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Estévez-Priego, Estefanía, primary, Teller, Sara, additional, Granell, Clara, additional, Arenas, Alex, additional, and Soriano, Jordi, additional
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- 2020
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9. Spontaneous Functional Recovery after Focal Damage in Neuronal Cultures
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Teller, Sara, primary, Estévez-Priego, Estefanía, additional, Granell, Clara, additional, Tornero, Daniel, additional, Andilla, Jordi, additional, Olarte, Omar E., additional, Loza-Alvarez, Pablo, additional, Arenas, Alex, additional, and Soriano, Jordi, additional
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- 2019
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10. HeNeCOn: An ontology for integrative research in Head and Neck cancer.
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Hernández, Liss, Estévez-Priego, Estefanía, López-Pérez, Laura, Fernanda Cabrera-Umpiérrez, María, Arredondo, María Teresa, and Fico, Giuseppe
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- 2024
- Full Text
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11. In VitroDevelopment of Human iPSC-Derived Functional Neuronal Networks on Laser-Fabricated 3D Scaffolds
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Koroleva, Anastasia, Deiwick, Andrea, El-Tamer, Ayman, Koch, Lothar, Shi, Yichen, Estévez-Priego, Estefanía, Ludl, Adriaan-Alexander, Soriano, Jordi, Guseva, Daria, Ponimaskin, Evgeni, and Chichkov, Boris
- Abstract
Neural progenitor cells generated from human induced pluripotent stem cells (hiPSCs) are the forefront of ″brain-on-chip″ investigations. Viable and functional hiPSC-derived neuronal networks are shaping powerful in vitromodels for evaluating the normal and abnormal formation of cortical circuits, understanding the underlying disease mechanisms, and investigating the response to drugs. They therefore represent a desirable instrument for both the scientific community and the pharmacological industry. However, culture conditions required for the full functional maturation of individual neurons and networks are still unidentified. It has been recognized that three-dimensional (3D) culture conditions can better emulate in vivoneuronal tissue development compared to 2D cultures and thus provide a more desirable in vitroapproach. In this paper, we present the design and implementation of a 3D scaffold platform that supports and promotes intricate neuronal network development. 3D scaffolds were produced through direct laser writing by two-photon polymerization (2PP), a high-resolution 3D laser microstructuring technology, using the biocompatible and nondegradable photoreactive resin Dental LT Clear (DClear). Neurons developed and interconnected on a 3D environment shaped by vertically stacked scaffold layers. The developed networks could support different cell types. Starting at the day 50 of 3D culture, neuronal progenitor cells could develop into cortical projection neurons (CNPs) of all six layers, different types of inhibitory neurons, and glia. Additionally and in contrast to 2D conditions, 3D scaffolds supported the long-term culturing of neuronal networks over the course of 120 days. Network health and functionality were probed through calcium imaging, which revealed a strong spontaneous neuronal activity that combined individual and collective events. Taken together, our results highlight advanced microstructured 3D scaffolds as a reliable platform for the 3D in vitromodeling of neuronal functions.
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- 2021
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12. Cambio de relación de aspecto en imágenes basado en contenido
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Estévez Priego, Estefanía, González Díaz, Iván, and Universidad Carlos III de Madrid. Departamento de Teoría de la Señal y Comunicaciones
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Telecomunicaciones ,Algoritmos ,Seams ,Tratamiento de imágenes ,Redimensión de imágenes - Abstract
Para redimensionar efectivamente una imagen no basta con atender a sus límites geométricos, sino que hay que tener en cuenta su contenido, ya que la importancia de una imagen reside en su interior, no en los bordes que la delimitan. En este documento se presenta un algoritmo inteligente, bautizado como Seam Carving, que permite cambiar la relación de aspecto de las imágenes priorizando la información relevante contenida en las mismas, evitando pérdidas innecesarias y deformaciones, tanto para reducciones como expansiones. Dicho algoritmo se basa en la idea de seams o caminos de píxeles consecutivos que van desde un extremo a otro de la imagen, ya sea de izquierda a derecha o de arriba abajo, dependiendo de la dimensión que se requiera transformar en cada momento. En nuestro caso, estos seams tienen la peculiaridad de portar la menor cantidad de energía dentro de la imagen. Es decir, se calculan de forma que contengan aquella información que resulta más despreciable o aquella que, por su redundancia con el resto del contenido, resulta menos perceptible. Así, Seam Carving ofrece mejores prestaciones y resultados frente a métodos convencionales de redimensionamiento o cambio de relación de aspecto, limitados por unas directrices que únicamente consideran la alteración de las proporciones de las imágenes desde un punto de vista estrictamente geométrico, ignorando las consecuencias que ocasiona esto en el contenido de las imágenes. La técnica desarrollada y evaluada en el presente Trabajo de Fin de Grado, permite aumentar o reducir la escala de una imagen para mejorar una composición, encajar un diseño o cambiar la orientación. Pero la implementación eficaz de este método puede utilizarse también para manipular la información propia del contenido y para eliminar elementos específicos de la imagen sin alterar el resto, por lo que se trata de una herramienta muy útil y versátil en el entorno de procesado de imagen. Ingeniería de Sistemas Audiovisuales
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- 2014
13. Spontaneous Functional Recovery after Focal Damage in Neuronal Cultures.
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Teller S, Estévez-Priego E, Granell C, Tornero D, Andilla J, Olarte OE, Loza-Alvarez P, Arenas A, and Soriano J
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- Animals, Rats, Rats, Sprague-Dawley, Central Nervous System injuries, Neurons pathology, Recovery of Function
- Abstract
Damage in biological neuronal networks triggers a complex functional reorganization whose mechanisms are still poorly understood. To delineate this reorganization process, here we investigate the functional alterations of in vitro rat cortical circuits following localized laser ablation. The analysis of the functional network configuration before and after ablation allowed us to quantify the extent of functional alterations and the characteristic spatial and temporal scales along recovery. We observed that damage precipitated a fast rerouting of information flow that restored network's communicability in about 15 min. Functional restoration was led by the immediate neighbors around trauma but was orchestrated by the entire network. Our in vitro setup exposes the ability of neuronal circuits to articulate fast responses to acute damage, and may serve as a proxy to devise recovery strategies in actual brain circuits. Moreover, this biological setup can become a benchmark to empirically test network theories about the spontaneous recovery in dynamical networks., (Copyright © 2020 Teller et al.)
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- 2020
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