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IntroductionWastewater treatment plants (WWTPs) and drinking-water treatment plants (DWTPs) are critical for public health due to the potential risks posed by microorganisms that may persist after treatment. The aim of this study was to detect the microbiome profiles of waters from both DWTPs and WWTPs under the Istanbul Water and Sewerage Administration (ISKI), identify the antimicrobial resistance profiles in all these facilities, and observe the differences in the microbiome between the inlet and outlet of different WWTPs.MethodsA total of 52 samples were examined, comprising 18 samples from DWTPs and 34 samples from WWTPs. All water samples underwent pre-isolation filtration. DNA isolation was conducted using filter material, followed by sequencing on a NovaSeq 6000 instrument. Kraken2 tools and R scripts were used for statistical analysis and data visualization.ResultsThe microbial metagenomic analysis identified 71 phyla, 113 classes, 217 orders, 480 families, and 1,282 genera across all samples. There were unclassified microbes (53.14% vs. 58.75%), Eukaryota (3.64% vs. 3.5%), Archaea (0.08% vs. 0.03%), bacteria (42% vs. 36.25%), and viruses (0.02% vs. 0.04%) in the raw water and ozonation unit outlet of DWTPs. The inlet and outlet of WWTPs showed unclassified microbes (52.68% vs. 59.62%), Eukaryota (0.6% vs. 1.72%), Archaea (0.26% vs. 0.15%), bacteria (46.43% vs. 38.43%), and viruses (0.05% vs. 0.04%). No statistically significant results were found in the analysis of raw waters collected from DWTPs and samples taken from the ozonation unit outlet—from the phylum level to the genus level (p > 0.05). The inlet and outlet points of WWTPs showed no statistically significant results from the phylum to species levels (p > 0.05). The most detected genera were Desulfobacter (4.82%) in preliminary WWTPs, Thauera (1.93%) in biological WWTPs, Pseudomonas (1.44%) in advanced biological WWTPs, Acidovorax (1.85%) in biological package WWTPs, and Pseudomonas (11.55%) in plant-based WWTPs. No antimicrobial resistance gene markers were detected in water samples from raw water inlets and ozonation unit outlets from DWTPs, membrane wastewater recovery plants, or ultraviolet (UV) recycling facilities. The ANT(3″), Erm, and Sul resistance gene markers were detected in all raw WWTPs samples.DiscussionThere were no significant microbial risk differentiation between biological WWTPs and advanced biological WWTPs. The data could serve as preliminary information for future research. More extensive studies are needed, with multiple sample tracking in these facilities and their feeding basins. more...

Bu çalışma Gökçeada çevresindeki anyonik deterjan ve fosfat kirliliğini belirlemek amacıyla Aralık 2016-Kasım 2017 tarihleri arasında yapılmıştır. Elde edilen sonuçlara göre çalışma dönemi boyunca Gökçeada çevresinde deterjan konsantrasyonları 0.020-0.051 mg/L arasında, fosfat konsantrasyonları ise 0.008-0.451 mg/L değerleri arasında bulunmuştur. Ada etrafından alınan su örneklerindeki ortalama anyonik deterjan ve fosfat konsantrasyonları ‘Su Kirliliği Kontrolü Yönetmeliği’ ne göre değerlendirilmiştir. Buna göre Gökçeada yüzey aktif madde açısından I. Sınıf (kirlenmemiş su), fosfat parametresi açısından ise II. Sınıf (az kirlenmiş su) olarak belirlenmiştir. more...