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Tamarind seed polysaccharide (TSP) is a neutral water-soluble galactoxyloglucan isolated from the seed kernel of Tamarindus indica with average molecular weight (Mw) 600–800 kDa. The high viscosity of TSP slows solubilisation, and the absence of charged substituent hinders the formation of electrostatic interactions with biomolecules. TSP was sulphated in a one-step process using dimethylformamide as a solvent, and sulphur trioxide-pyridine complex as a sulphating reagent. Studies of chemical structure, molecular weight distribution and viscosity were conducted to characterise the synthesised products. The sulphation degree was established by conductimetric titration; the sulphate group distribution was studied by NMR spectroscopy and liquid chromatography-mass spectrometry, and sulphated TSP oligomers were obtained by enzymatic degradation with cellulase and/or xyloglucanase. Sulphated products showed higher solubility than TSP, Mws in the range of 700–1000 kDa, a sulphation degree of two to four per subunit and pseudoplastic behaviour. A preliminary study of mucoadhesion revealed the unexpected interaction of S-TSP with mucin, providing a route by which sulphated TSP interactions with biomolecules may be influenced. [ABSTRACT FROM AUTHOR]

The public and academic debate about urban squatting in Western cities has been dominated by research on collectively organized, politically-motivated occupations. By contrast, occupations promoted to fulfil urgent housing needs by uncoordinated urban poor without any connection with activists (i.e. need-based squatting) have been far less explored. The present paper contributes to filling this research gap concerning squatting as a sheltering strategy by marginalized individuals. To this end, this article focuses on the overlooked phenomenon of the illegal occupation of public buildings for residential purposes in Italy that occurs outside any explicit political framework. In particular, it provides an ethnographic investigation of a case of squatting in an abandoned school located in a public housing neighbourhood in Naples. This investigation is the basis for the conceptualization of a specific type of need-based squatting, that is to say 'individualistic squatting', whose specific features (including its distinct political character) are highlighted, together with its peculiarity vis-à-vis other types of need-based squatting. [ABSTRACT FROM AUTHOR]