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Your search keyword '"Espinoza-Sánchez, Nancy Adriana"' showing total 15 results
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15 results on '"Espinoza-Sánchez, Nancy Adriana"'

1. Role of Syndecan-1 in Cancer Stem Cells

Author

Ibrahim, Sherif Abdelaziz, Hassan, Hebatallah, Reinbold, Rolland, Espinoza-Sanchez, Nancy Adriana, Greve, Burkhard, Götte, Martin, Karamanos, Nikos K., Series Editor, Kletsas, Dimitris, Editorial Board Member, Oh, Eok-Soo, Editorial Board Member, Passi, Alberto, Editorial Board Member, Pihlajaniemi, Taina, Editorial Board Member, Ricard-Blum, Sylvie, Editorial Board Member, Sagi, Irit, Editorial Board Member, Savani, Rashmin, Editorial Board Member, Watanabe, Hideto, Editorial Board Member, Götte, Martin, editor, and Forsberg-Nilsson, Karin, editor

Published
2021
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3. Antioxidants Hydroxytyrosol and Thioredoxin-Mimetic Peptide CB3 Protect Irradiated Normal Tissue Cells.

Author

Borrmann, Katrin, Troschel, Fabian Martin, Brücksken, Kathrin Annemarie, Espinoza-Sánchez, Nancy Adriana, Rezaei, Maryam, Eder, Kai Moritz, Kemper, Björn, Eich, Hans Theodor, and Greve, Burkhard

Subjects
DIGITAL holographic microscopy, REACTIVE oxygen species, POLYMERASE chain reaction, UMBILICAL veins, IONIZING radiation, WOUND healing
Abstract

Reducing side effects in non-cancerous tissue is a key aim of modern radiotherapy. Here, we assessed whether the use of the antioxidants hydroxytyrosol (HT) and thioredoxin-mimetic peptide CB3 (TMP) attenuated radiation-induced normal tissue toxicity in vitro. We used primary human umbilical vein endothelial cells (HUVECs) and human epidermal keratinocytes (HaCaT) as normal tissue models. Cells were treated with HT and TMP 24 h or immediately prior to irradiation. Reactive oxygen species (ROS) were assessed via luminescent- and fluorescence-based assays, migration was investigated using digital holographic microscopy, and clonogenic survival was quantified by colony formation assays. Angiogenesis and wound healing were evaluated via time-dependent microscopy. Secreted cytokines were validated in quantitative polymerase chain reaction (qPCR) studies. Treatment with HT or TMP was well tolerated by cells. The application of either antioxidant before irradiation resulted in reduced ROS formation and a distinct decrease in cytokines compared to similarly irradiated, but otherwise untreated, controls. Antioxidant treatment also increased post-radiogenic migration and angiogenesis while accelerating wound healing. HT or TMP treatment immediately before radiotherapy increased clonogenic survival after radiotherapy, while treatment 24 h before radiotherapy enhanced baseline proliferation. Both antioxidants may decrease radiation-induced normal tissue toxicity and deserve further pre-clinical investigation. [ABSTRACT FROM AUTHOR]

Published
2024
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5. SETD3 acts as a prognostic marker in breast cancer patients and modulates the viability and invasion of breast cancer cells

Author

Hassan, Nourhan, Rutsch, Niklas, Győrffy, Balázs, Espinoza-Sánchez, Nancy Adriana, Götte, Martin, and Universitäts- und Landesbibliothek Münster

Subjects
Cell Survival, Receptor, ErbB-2, lcsh:Medicine, Breast Neoplasms, Kaplan-Meier Estimate, Article, Disease-Free Survival, Epigenesis, Genetic, Prognostic markers, Mechanisms of disease, Cell Line, Tumor, Biomarkers, Tumor, Humans, Neoplasm Invasiveness, Breast, ddc:610, RNA, Small Interfering, lcsh:Science, skin and connective tissue diseases, lcsh:R, DNA Methylation, Prognosis, Gene Expression Regulation, Neoplastic, Receptors, Estrogen, Gene Knockdown Techniques, Mutation, Medicine and health, Disease Progression, Histone Methyltransferases, lcsh:Q, Female, Neoplasm Recurrence, Local, Tumor Suppressor Protein p53, Receptors, Progesterone
Abstract

In several carcinomas, the SET Domain Containing 3, Actin Histidine Methyltransferase (SETD3) is associated with oncogenesis. However, there is little knowledge about the role of SETD3 in the progression and prognosis of breast cancer. In this study, we first analyzed the prognostic value of SETD3 in breast cancer patients using the database of the public Kaplan-Meier plotter. Moreover, in vitro assays were performed to assess the role of SETD3 in the viability and capacity of invasion of human breast cancer cell lines. We observed that the high expression of SETD3 was associated with better relapse-free survival (RFS) of the whole collective of 3,951 patients, of Estrogen Receptor-positive, and of Luminal A-type breast cancer patients. However, in patients lacking expression of estrogen-, progesterone- and HER2-receptor, and those affected by a p53-mutation, SETD3 was associated with poor RFS. In vitro analysis showed that SETD3 siRNA depletion affects the viability of triple-negative cells as well as the cytoskeletal function and capacity of invasion of highly invasive MDA-MB-231 cells. Interestingly, SETD3 regulates the expression of other genes associated with cancer such as β-actin, FOXM1, FBXW7, Fascin, eNOS, and MMP-2. Our study suggests that SETD3 expression can act as a subtype-specific biomarker for breast cancer progression and prognosis.

Published
2021

6. Syndecan-1 Depletion Has a Differential Impact on Hyaluronic Acid Metabolism and Tumor Cell Behavior in Luminal and Triple-Negative Breast Cancer Cells

Author

Valla, Sofía, Hassan, Nourhan, Luján Vitale, Daiana, Madanes, Daniela, Spinelli, Fiorella Mercedes, Teixeira, Felipe C. O. B., Greve, Burkhard, Espinoza-Sánchez, Nancy Adriana, Cristina, Carolina, Alaniz, Laura, Götte, Martin, and Universitäts- und Landesbibliothek Münster

Subjects
Databases, Factual, QH301-705.5, 610 Medicine and health, Kruppel-Like Transcription Factors, Triple Negative Breast Neoplasms, Glycocalyx, Article, Kruppel-Like Factor 4, angiogenesis, breast cancer, stem cells, Cell Line, Tumor, hyaluronic acid, Humans, ddc:610, RNA, Small Interfering, Biology (General), skin and connective tissue diseases, Wnt Signaling Pathway, QD1-999, Mitogen-Activated Protein Kinase 1, syndecan-1, apoptosis, Mitogen-Activated Protein Kinase 3, Cell Cycle, PTEN Phosphohydrolase, CD24 Antigen, RNA-Binding Proteins, Survival Analysis, Gene Expression Regulation, Neoplastic, MicroRNAs, Chemistry, Hyaluronan Receptors, Medicine and health, MCF-7 Cells, Female, Hyaluronan Synthases, Proto-Oncogene Proteins c-akt, Protein Binding
Abstract

Glycosaminoglycans (GAGs) and proteoglycans (PGs) are major components of the glycocalyx. The secreted GAG and CD44 ligand hyaluronic acid (HA), and the cell surface PG syndecan-1 (Sdc-1) modulate the expression and activity of cytokines, chemokines, growth factors, and adhesion molecules, acting as critical regulators of tumor cell behavior. Here, we studied the effect of Sdc-1 siRNA depletion and HA treatment on hallmark processes of cancer in breast cancer cell lines of different levels of aggressiveness. We analyzed HA synthesis, and parameters relevant to tumor progression, including the stem cell phenotype, Wnt signaling constituents, cell cycle progression and apoptosis, and angiogenic markers in luminal MCF-7 and triple-negative MDA-MB-231 cells. Sdc-1 knockdown enhanced HAS-2 synthesis and HA binding in MCF-7, but not in MDA-MB-231 cells. Sdc-1-depleted MDA-MB-231 cells showed a reduced CD24-/CD44+ population. Furthermore, Sdc-1 depletion was associated with survival signals in both cell lines, affecting cell cycle progression and apoptosis evasion. These changes were linked to the altered expression of KLF4, MSI2, and miR-10b and differential changes in Erk, Akt, and PTEN signaling. We conclude that Sdc-1 knockdown differentially affects HA metabolism in luminal and triple-negative breast cancer model cell lines and impacts the stem phenotype, cell survival, and angiogenic factors., Finanziert durch den Open-Access-Publikationsfonds der Westfälischen Wilhelms-Universität Münster (WWU Münster).

Published
2021

15. Monocyte Differentiation towards Protumor Activity Does Not Correlate with M1 or M2 Phenotypes.

Author

Chimal-Ramírez, G. Karina, Espinoza-Sánchez, Nancy Adriana, Chávez-Sánchez, Luis, Arriaga-Pizano, Lourdes, Fuentes-Pananá, Ezequiel M., Chimal-Ramírez, G Karina, Espinoza-Sánchez, Nancy Adriana, Chávez-Sánchez, Luis, and Fuentes-Pananá, Ezequiel M

Subjects
*MONOCYTES, *CELL differentiation, *BREAST cancer, *CANCER invasiveness, *MACROPHAGES, *CD86 antigen, *INDOLEAMINE 2,3-dioxygenase, *CANCER cells, *ANTIGENS, *BIOCHEMISTRY, *CELL culture, *CELL lines, *CELL receptors, *CYTOKINES, *FLOW cytometry, *HYDROLASES, *INTERLEUKINS, *PHENOMENOLOGY, *OXIDOREDUCTASES, *TUMOR necrosis factors, *PHENOTYPES, *PHYSIOLOGY
Abstract

Macrophages facilitate breast cancer progression. Macrophages were initially classified as M1 or M2 based on their distinct metabolic programs and then expanded to include antitumoral (M1) and protumoral (M2) activities. However, it is still uncertain what markers define the pro- and antitumoral phenotypes and what conditions lead to their formation. In this study, monocytic cell lines and primary monocytes were subjected to commonly reported protocols of M1/M2 polarization and conditions known to engage monocytes into protumoral functions. The results showed that only IDO enzyme and CD86 M1 markers were upregulated correlating with M1 polarization. TNF-α, CCR7, IL-10, arginase I, CD36, and CD163 were expressed indistinguishably from M1 or M2 polarization. Similarly, protumoral engaging resulted in upregulation of both M1 and M2 markers, with conditioned media from the most aggressive breast cancer cell line promoting the greatest changes. In spite of the mixed phenotype, M1-polarized macrophages exhibited the highest expression/secretion of inflammatory mediators, many of which have previously been associated with breast cancer aggressiveness. These data argue that although the existence of protumoral macrophages is unquestionable, their associated phenotypes and the precise conditions driving their formation are still unclear, and those conditions may need both M1 and M2 stimuli. [ABSTRACT FROM AUTHOR]

Published
2016
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