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3. Architecture for Predictive Maintenance Based on Integrated Models, Methods and Technologies

Author

Werner, Andreas, Mendez-Rial, Roi, Salvo, Pablo, Charisi, Vasiliki, Piccini, Joaquín, Mousavi, Alireza, Civardi, Claudio, Monios, Nikos, Espinosa, Diego Bartolomé, Hildebrand, Marlène, Zimmermann, Nikolas, Aguirre, Irati Vizcarguenaga, Cassina, Jacopo, Avendano, Diego Nieves, Oliveira, Helder, Caljouw, Daniel, Fazziani, Matteo, de la Maza, Silvia, Behrens, Bernd-Arno, Series Editor, Grzesik, Wit, Series Editor, Ihlenfeldt, Steffen, Series Editor, Kara, Sami, Series Editor, Ong, Soh-Khim, Series Editor, Tomiyama, Tetsuo, Series Editor, Williams, David, Series Editor, Huang, Chin-Yin, editor, Dekkers, Rob, editor, Chiu, Shun Fung, editor, Popescu, Daniela, editor, and Quezada, Luis, editor

Published
2023
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4. Structural basis for antibody inhibition of flavivirus NS1–triggered endothelial dysfunction

Author

Biering, Scott B, Akey, David L, Wong, Marcus P, Brown, W Clay, Lo, Nicholas TN, Puerta-Guardo, Henry, Tramontini Gomes de Sousa, Francielle, Wang, Chunling, Konwerski, Jamie R, Espinosa, Diego A, Bockhaus, Nicholas J, Glasner, Dustin R, Li, Jeffrey, Blanc, Sophie F, Juan, Evan Y, Elledge, Stephen J, Mina, Michael J, Beatty, P Robert, Smith, Janet L, and Harris, Eva

Subjects
Vaccine Related, Prevention, Immunization, Vector-Borne Diseases, Rare Diseases, Emerging Infectious Diseases, Biodefense, Infectious Diseases, West Nile Virus, Aetiology, 2.1 Biological and endogenous factors, Infection, Good Health and Well Being, Animals, Antibodies, Monoclonal, Antibodies, Neutralizing, Antibodies, Viral, Cross Reactions, Crystallography, X-Ray, Dengue, Dengue Virus, Endothelium, Glycocalyx, Humans, Mice, Protein Conformation, beta-Strand, Protein Domains, Viral Nonstructural Proteins, West Nile Fever, West Nile virus, Zika Virus, Zika Virus Infection, General Science & Technology
Abstract

Medically important flaviviruses cause diverse disease pathologies and collectively are responsible for a major global disease burden. A contributing factor to pathogenesis is secreted flavivirus nonstructural protein 1 (NS1). Despite demonstrated protection by NS1-specific antibodies against lethal flavivirus challenge, the structural and mechanistic basis remains unknown. Here, we present three crystal structures of full-length dengue virus NS1 complexed with a flavivirus-cross-reactive, NS1-specific monoclonal antibody, 2B7, at resolutions between 2.89 and 3.96 angstroms. These structures reveal a protective mechanism by which two domains of NS1 are antagonized simultaneously. The NS1 wing domain mediates cell binding, whereas the β-ladder triggers downstream events, both of which are required for dengue, Zika, and West Nile virus NS1-mediated endothelial dysfunction. These observations provide a mechanistic explanation for 2B7 protection against NS1-induced pathology and demonstrate the potential of one antibody to treat infections by multiple flaviviruses.

Published
2021

5. Interferon receptor-deficient mice are susceptible to eschar-associated rickettsiosis

Author

Burke, Thomas P, Engström, Patrik, Tran, Cuong J, Langohr, Ingeborg M, Glasner, Dustin R, Espinosa, Diego A, Harris, Eva, and Welch, Matthew D

Subjects
Vaccine Related, Prevention, Biodefense, Infectious Diseases, Vector-Borne Diseases, Rare Diseases, Emerging Infectious Diseases, Aetiology, 2.1 Biological and endogenous factors, Infection, Good Health and Well Being, Animals, Bone Marrow, Female, Genetic Association Studies, Immunity, Innate, Inflammation, Listeria monocytogenes, Macrophages, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, Receptor, Interferon alpha-beta, Receptors, Interferon, Rickettsia, Rickettsia Infections, Ticks, actin-based motility, eschar-associated rickettsiosis, immunity, infectious disease, interferons, listeria monocytogenes, microbiology, mouse, rickettsia, rickettsia parkeri, type i interferon, Biochemistry and Cell Biology
Abstract

Arthropod-borne rickettsial pathogens cause mild and severe human disease worldwide. The tick-borne pathogen Rickettsia parkeri elicits skin lesions (eschars) and disseminated disease in humans; however, inbred mice are generally resistant to infection. We report that intradermal infection of mice lacking both interferon receptors (Ifnar1-/-;Ifngr1-/-) with as few as 10 R. parkeri elicits eschar formation and disseminated, lethal disease. Similar to human infection, eschars exhibited necrosis and inflammation, with bacteria primarily found in leukocytes. Using this model, we find that the actin-based motility factor Sca2 is required for dissemination from the skin to internal organs, and the outer membrane protein OmpB contributes to eschar formation. Immunizing Ifnar1-/-;Ifngr1-/- mice with sca2 and ompB mutant R. parkeri protects against rechallenge, revealing live-attenuated vaccine candidates. Thus, Ifnar1-/-;Ifngr1-/- mice are a tractable model to investigate rickettsiosis, virulence factors, and immunity. Our results further suggest that discrepancies between mouse and human susceptibility may be due to differences in interferon signaling.

Published
2021

6. Identification of Dengue Virus Serotype 3 Specific Antigenic Sites Targeted by Neutralizing Human Antibodies

Author

Young, Ellen, Carnahan, Robert H, Andrade, Daniela V, Kose, Nurgun, Nargi, Rachel S, Fritch, Ethan J, Munt, Jennifer E, Doyle, Michael P, White, Laura, Baric, Thomas J, Stoops, Mark, DeSilva, Aravinda, Tse, Longping V, Martinez, David R, Zhu, Deanna, Metz, Stefan, Wong, Marcus P, Espinosa, Diego A, Montoya, Magelda, Biering, Scott B, Sukulpolvi-Petty, Soila, Kuan, Guillermina, Balmaseda, Angel, Diamond, Michael S, Harris, Eva, Crowe, James E, and Baric, Ralph S

Subjects
Clinical Research, Immunization, Biotechnology, Biodefense, Infectious Diseases, Emerging Infectious Diseases, Prevention, Rare Diseases, Vector-Borne Diseases, Vaccine Related, Infection, Good Health and Well Being, Adolescent, Animals, Antibodies, Monoclonal, Antibodies, Neutralizing, Antibodies, Viral, B-Lymphocytes, Child, Child, Preschool, Chlorocebus aethiops, Dengue, Dengue Vaccines, Dengue Virus, Epitope Mapping, Epitopes, Humans, Mice, Models, Molecular, Nicaragua, Sequence Alignment, Serogroup, Vero Cells, Viral Envelope Proteins, Virion, DENV3, antigenic site, chimeric virus, dengue virus serotype 3, envelope protein, flavivirus, functional epitopes, in vivo protection, neutralizing monoclonal antibodies, Microbiology, Medical Microbiology, Immunology
Abstract

The rational design of dengue virus (DENV) vaccines requires a detailed understanding of the molecular basis for antibody-mediated immunity. The durably protective antibody response to DENV after primary infection is serotype specific. However, there is an incomplete understanding of the antigenic determinants for DENV type-specific (TS) antibodies, especially for DENV serotype 3, which has only one well-studied, strongly neutralizing human monoclonal antibody (mAb). Here, we investigated the human B cell response in children after natural DENV infection in the endemic area of Nicaragua and isolated 15 DENV3 TS mAbs recognizing the envelope (E) glycoprotein. Functional epitope mapping of these mAbs and small animal prophylaxis studies revealed a complex landscape with protective epitopes clustering in at least 6-7 antigenic sites. Potently neutralizing TS mAbs recognized sites principally in E glycoprotein domains I and II, and patterns suggest frequent recognition of quaternary structures on the surface of viral particles.

Published
2020

7. Cyclic Dinucleotide–Adjuvanted Dengue Virus Nonstructural Protein 1 Induces Protective Antibody and T Cell Responses

Author

Espinosa, Diego A, Beatty, P Robert, Reiner, Gabrielle L, Sivick, Kelsey E, Hix Glickman, Laura, Dubensky, Thomas W, and Harris, Eva

Subjects
Biotechnology, Emerging Infectious Diseases, Prevention, Biodefense, Infectious Diseases, Rare Diseases, Immunization, Vector-Borne Diseases, Vaccine Related, 2.1 Biological and endogenous factors, Aetiology, Infection, Good Health and Well Being, Adjuvants, Immunologic, Animals, Antibodies, Viral, Dengue Virus, Mice, Mice, Inbred C57BL, Mice, Knockout, Nucleotides, Cyclic, T-Lymphocytes, Viral Nonstructural Proteins, Immunology
Abstract

Endothelial dysfunction and vascular leak, pathogenic hallmarks of severe dengue disease, are directly triggered by dengue virus (DENV) nonstructural protein 1 (NS1). Previous studies have shown that immunization with NS1, as well as passive transfer of NS1-immune serum or anti-NS1 mAb, prevent NS1-mediated lethality in vivo. In this study, we evaluated the immunogenicity and protective capacity of recombinant DENV NS1 administered with cyclic dinucleotides (CDNs), potent activators of innate immune pathways and highly immunogenic adjuvants. Using both wild-type C57BL/6 mice and IFN-α/β receptor-deficient mice, we show that NS1-CDN immunizations elicit serotype-specific and cross-reactive Ab and T cell responses. Furthermore, NS1-CDN vaccinations conferred significant homotypic and heterotypic protection from DENV2-induced morbidity and mortality. In addition, we demonstrate that high anti-NS1 Ab titers are associated with protection, supporting the role of humoral responses against DENV NS1 as correlates of protection. These findings highlight the potential of CDN-based adjuvants for inducing Ab and T cell responses and validate NS1 as an important candidate for dengue vaccine development.

Published
2019

8. Flavivirus NS1 Triggers Tissue-Specific Vascular Endothelial Dysfunction Reflecting Disease Tropism

Author

Puerta-Guardo, Henry, Glasner, Dustin R, Espinosa, Diego A, Biering, Scott B, Patana, Mark, Ratnasiri, Kalani, Wang, Chunling, Beatty, P Robert, and Harris, Eva

Subjects
Prevention, West Nile Virus, Vaccine Related, Immunization, Biodefense, Emerging Infectious Diseases, Infectious Diseases, Rare Diseases, Vector-Borne Diseases, 2.1 Biological and endogenous factors, Aetiology, Infection, Good Health and Well Being, Animals, Brain, Cell Line, Cell Membrane Permeability, Dengue, Dengue Virus, Dermis, Encephalitis Virus, Japanese, Endothelial Cells, Gene Expression, Glycocalyx, Humans, Liver, Lung, Male, Mice, Organ Specificity, Primary Cell Culture, Umbilical Veins, Viral Nonstructural Proteins, Virus Replication, West Nile virus, Yellow fever virus, Zika Virus, Flavivirus, Japanese encephalitis virus, NS1, Zika virus, dengue virus, disease tropism, endothelial permeability, vascular leak, yellow fever virus, Biochemistry and Cell Biology, Medical Physiology
Abstract

Flaviviruses cause systemic or neurotropic-encephalitic pathology in humans. The flavivirus nonstructural protein 1 (NS1) is a secreted glycoprotein involved in viral replication, immune evasion, and vascular leakage during dengue virus infection. However, the contribution of secreted NS1 from related flaviviruses to viral pathogenesis remains unknown. Here, we demonstrate that NS1 from dengue, Zika, West Nile, Japanese encephalitis, and yellow fever viruses selectively binds to and alters permeability of human endothelial cells from lung, dermis, umbilical vein, brain, and liver in vitro and causes tissue-specific vascular leakage in mice, reflecting the pathophysiology of each flavivirus. Mechanistically, each flavivirus NS1 leads to differential disruption of endothelial glycocalyx components, resulting in endothelial hyperpermeability. Our findings reveal the capacity of a secreted viral protein to modulate endothelial barrier function in a tissue-specific manner both in vitro and in vivo, potentially influencing virus dissemination and pathogenesis and providing targets for antiviral therapies and vaccine development.

Published
2019

9. Methodological Proposal for Privilege Escalation in Windows Systems

Author

Espinosa, Diego Muñoz, Vidal, David Cordero, Huidobro, Cristian Barría, Filipe, Joaquim, Editorial Board Member, Ghosh, Ashish, Editorial Board Member, Prates, Raquel Oliveira, Editorial Board Member, Zhou, Lizhu, Editorial Board Member, Mata-Rivera, Miguel Félix, editor, and Zagal-Flores, Roberto, editor

Published
2021
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13. Hidradenitis supurativa, una actualización. Parte II: diagnóstico, clasificación y tratamiento.

Author

Guillermo Pabón, Juan, Flaminio Rojas, Ricardo, Barona, María Isabel, Orozco, Beatriz, Meléndez, Esperanza, Beatriz Jiménez, Sol, Motta, Adriana, Johana Rueda, Lili, González, Claudia, Ibagón, Viviana, Vallejo, Jessica, Armand, Beatriz, Avellaneda, María del Pilar, Bendek, Edwin, Montealegre, Carlos, Velásquez, Natalia, Quiroz, Lina, Uribe, Claudia, Lotero, Cristina, and Espinosa, Diego

Subjects
HIDRADENITIS suppurativa, QUALITY of life, ULTRASONIC imaging, IMMUNE response, DIAGNOSIS
Abstract

Copyright of Revista de la Asociación Colombiana de Dermatología y Cirugía Dermatológica is the property of Asociacion Colombiana de Dermatologia y Cirugia Dermatologica and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2024
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16. Novel CRISPR-Associated Gene-Editing Systems Discovered in Metagenomic Samples Enable Efficient and Specific Genome Engineering

Author

Lamothe, Rebecca C., primary, Storlie, Meghan D., additional, Espinosa, Diego A., additional, Rudlaff, Rachel, additional, Browne, Patrick, additional, Liu, Jason, additional, Rivas, Andres, additional, Devoto, Audra, additional, Oki, Jennifer, additional, Khoubyari, Ashcon, additional, Goltsman, Daniela S. Aliaga, additional, Lin, Jyun-Liang, additional, Butterfield, Cristina N., additional, Brown, Christopher T., additional, Thomas, Brian C., additional, and Cost, Gregory J., additional

Published
2023
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17. Specificity, cross-reactivity, and function of antibodies elicited by Zika virus infection

Author

Stettler, Karin, Beltramello, Martina, Espinosa, Diego A., Graham, Victoria, Cassotta, Antonino, Bianchi, Siro, Vanzetta, Fabrizia, Minola, Andrea, Jaconi, Stefano, Mele, Federico, Foglierini, Mathilde, Pedotti, Mattia, Simonelli, Luca, Dowall, Stuart, Atkinson, Barry, Percivalle, Elena, Simmons, Cameron P., Varani, Luca, Blum, Johannes, Baldanti, Fausto, Cameroni, Elisabetta, Hewson, Roger, Harris, Eva, Lanzavecchia, Antonio, Sallusto, Federica, and Corti, Davide

Published
2016

19. Physiology and biochemistry of naranjilla (Solanum quitoense Lam) fruit during postharvest and the main conservation strategies: A review.

Author

Reyes Medina, Andrea Johana, Castellanos Espinosa, Diego Alberto, and Enrique Balaguera-López, Helber

Subjects
PHYSIOLOGY, BIOCHEMISTRY, ANDEANS (South American people), VITAMIN C, ETHYLENE
Abstract

Copyright of Agronomía Colombiana is the property of Universidad Nacional de Colombia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2023
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21. Multisystem Inflammatory Syndrome in Children (MIS-C) temporally related to COVID-19: the experience at a pediatric reference hospital in Colombia

Author

Lozano-Espinosa, Diego Alejandro, primary, Camacho-Moreno, Germán, additional, López-Cubillos, Juan Francisco, additional, Díaz-Maldonado, Adriana Soraya, additional, León-Guerra, Oscar Javier, additional, Galvis-Trujillo, Diego Mauricio, additional, Sanguino-Lobo, Roy, additional, Arévalo-Leal, Oscar Guillermo, additional, del Castillo, Ana María Eraso-Díaz, additional, Reina-Ávila, María Fernanda, additional, Cárdenas-Hernández, Vicky Carolina, additional, Ivankovich-Escoto, Gabriela, additional, Tremoulet, Adriana H, additional, and Ulloa-Gutiérrez, Rolando, additional

Published
2023
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24. Multisystem Inflammatory Syndrome in Children (MIS-C) temporally related to COVID-19: the experience at a pediatric reference hospital in Colombia

Author

Lozano-Espinosa, Diego Alejandro, Camacho-Moreno, Germán, López-Cubillos, Juan Francisco, Díaz-Maldonado, Adriana Soraya, León-Guerra, Oscar Javier, Galvis-Trujillo, Diego Mauricio, Sanguino-Lobo, Roy, Arévalo-Leal, Oscar Guillermo, del Castillo, Ana María Eraso-Díaz, Reina-Ávila, María Fernanda, Cárdenas-Hernández, Vicky Carolina, Ivankovich-Escoto, Gabriela, Tremoulet, Adriana H, and Ulloa-Gutiérrez, Rolando

Subjects
Doença de Kawasaki, Kawasaki disease, Crianças, Disfunção ventricular, COVID-19, Multisystem inflammatory syndrome, Falência de múltiplos órgãos, Child, Ventricular dysfunction, Multiple organ failure, Síndrome inflamatória multissistêmica
Abstract

Objective: This study aimed to describe the clinical characteristics and the different phenotypes of children with multisystem inflammatory syndrome in children (MIS-C) temporally related to COVID-19 and to evaluate the risk conditions that favored a greater severity of the disease during a 12-month period at a pediatric reference hospital in Colombia. Methods: A 12-month retrospective observational study of children under the age of 18 years who met criteria for MIS-C. Results: A total of 28 children presented MIS-C criteria. The median age was 7 years. Other than fever (100%) (onset 4 days prior to admission), the most frequent clinical features were gastrointestinal (86%) and mucocutaneous (61%). Notably, 14 (50%) children had Kawasaki-like symptoms. The most frequent echocardiographic abnormalities were pericardial effusion (64%), valvular involvement (68%), ventricular dysfunction (39%), and coronary artery abnormalities (29%). In addition, 75% had lymphopenia. All had at least one abnormal coagulation test. Most received intravenous immunoglobulin (89%), glucocorticoids (82%), vasopressors (54%), and antibiotics (64%). Notably, 61% had a more severe form of the disease and were admitted to an intensive care unit (median 4 days, mean 6 days); the severity predictors were patients with the inflammatory/MIS-C phenotype (OR 26.5; 95%CI 1.40–503.7; p=0.029) and rash (OR 14.7; 95%CI 1.2–178.7; p=0.034). Two patients had macrophage activation syndrome. Conclusions: Coronary artery abnormalities, ventricular dysfunction, and intensive care unit admission were frequent, which needs to highlight the importance of early clinical suspicion. Resumo Objetivo: Descrever as características clínicas e os diferentes fenótipos de crianças com síndrome inflamatória multissistêmica na criança temporalmente relacionada com a COVID-19 (do inglês multisystem inflammatory syndrome in children — MIS-C) e avaliar as condições de risco que favorecem a maior gravidade da doença durante um período de 12 meses em um hospital pediátrico de referência na Colômbia. Métodos: Estudo retrospectivo de 12 meses de observação de crianças menores de 18 anos que cumprem os critérios para o MIS-C. Resultados: Vinte e oito crianças foram apresentadas com os critérios do MIS-C. A idade média era de sete anos, e 54% eram do sexo masculino. Para além da febre (100%) (com início quatro dias antes da admissão), as características clínicas mais frequentes eram gastrointestinais (86%) e mucocutâneas (61%). Quatorze crianças (50%) apresentavam sintomas semelhantes aos de Kawasaki. As anomalias ecocardiográficas mais frequentes foram derrame pericárdico (64%), envolvimento valvar (68%), disfunção ventricular (39%) e anomalias coronárias (29%). Tinham linfopenia 75% das crianças. Todas tinham algum teste de coagulação anormal. A maioria recebeu imunoglobulina intravenosa (89%), glucocorticoides (82%), vasopressores (54%) e antibióticos (64%). Tiveram envolvimento mais grave 61% dos pacientes, que precisaram ser internados em unidade de terapia intensiva (mediana de quatro dias, média de seis dias); os preditores de gravidade foram pacientes com fenótipo inflamatório/ MIS-C (odds ratio — OR 26,5; intervalo de confiança — IC95% 1,4–503,7; p=0,029) e erupção cutânea (OR 14,7; IC95% 1,2–178,7; p=0,034). Dois pacientes (7%) apresentavam síndrome de ativação macrofágica. Conclusões: Alteração da artéria coronária, disfunção ventricular e internação na unidade de terapia intensiva foram frequentes, o que nos alerta sobre a importância da suspeita clínica precoce.

Published
2022

29. Aplicación móvil para la gestión de basuras de un núcleo urbano

Author

Martinez Espinosa, Diego Alejandro and Hernández Díaz, Vicente

Subjects
Informática
Abstract

Este proyecto se realizó con el fin de diseñar y desarrollar un sistema que, mediante una aplicación móvil en Android, permita a los administradores de las empresas encargadas de la recolección de basuras de un núcleo urbano y a sus usuarios, disponer de información precisa y en tiempo real del lugar en donde están los contenedores, en qué porcentaje de llenado se encuentran y el trazado de las posibles mejores rutas que se pueden tomar para la recolección o depósito de las basuras. Con ello se pretende evitar pérdidas de tiempo, mala disposición de las basuras y contaminación del medio ambiente. La información suministrada se obtuvo del Portal de Datos Abiertos del Ayuntamiento de Madrid y de la simulación de sensores que enviaban información a una plataforma IoT (Internet Of Things) para su almacenamiento y administración, haciendo posible así que todos los usuarios de la aplicación móvil pudieran tener acceso a ella y lograr una ciudad más inteligente y limpia.

Published
2022

30. Procesos agroindustriales en mora de Castilla

Author

Díaz Ortiz, Alberto, Ochoa Martínez, Claudia Isabel, Vélez Pasos, Carlos, Ayala Aponte, Alfredo Adolfo, Fernández Quintero, Alejandro, Delgado Solarte, Diana María, Plaza Cortés, Cindy Camila, Terán Llorente, Diana Milena, González Torres, Vanessa, González Hoyos, Lina Vanessa, Benítez Franco, Juan Pablo, Vargas Serna, Claudia Liliana, Escobar Espinal, Paula Andrea, Castellanos Espinosa, Diego Alberto, Moncayo Martínez, Diana Cristina, Barón Cangrejo, Omar Giovanny, Posada Rubiano, Nestor Camilo, Rada Bula, Adriana Isabel, Cortés Jaimes, Lesley Andrea, Lopera Sepúlveda, Alex Arbey, Universidad del Valle, Parque BioPacífico, Centro Internacional de Agricultura Tropical - CIAT, Bioversity International, Sistema General de Regalías - SGR, Gobernación del Valle del Cauca, and Universidad Nacional de Colombia

Subjects
Fruit quality, 664 - Tecnología de alimentos [660 - Ingeniería química], Fruit growing, Research, Commercialization, Agroindustria, Investigaciones, Mulberries, Colombia, Agroindustry, Mora, Valle del Cauca, Proyectos, Product development, Fruticultura, Tecnología postcosecha, Mountain farming, Packaging, Agricultura de montaña, Agro-industry, Vigilancia tecnológica, Empaquetado, Comercialización, Morus, Desarrollo de un producto
Abstract

Ilustraciones (principalmente a color), diagramas, figuras, fotografías, mapas Dentro del proyecto BPIN 2014000100010 “Incremento de la competitividad sostenible en la agricultura de ladera en todo el departamento, Valle del Cauca, Occidente”, la Universidad del Valle propuso la actividad 2: diseñar e implementar procesos agroindustriales que generen, con base en estándares internacionales, valor agregado a los productos seleccionados, así como desarrollar y/o evaluar empaques innovadores apropiados para los productos seleccionados en fresco y procesados. Esta actividad incluye las siguientes acciones específicas: 1) vigilancia tecnológica sobre procesos agroindustriales y productos; 2) selección de productos procesados a desarrollar y 3) selección de los procesos, entre otras. Este documento corresponde al entregable de las actividades específicas 1, 2 y 3. Se incluyen los resultados de los productos y los procesos seleccionados de acuerdo con las brechas identificadas en mora de Castilla, tanto en fresco como procesada, tras un análisis comparativo de los resultados. La vigilancia tecnológica se llevó a cabo incluyendo cuatro tipos de vigilancias (competitiva, comercial, científico-tecnológica y estratégica). Con base en estas, se seleccionaron los productos y procesos correspondientes a la fruta objeto de este estudio. A partir de lo anterior, se evidenció que aumentar la vida útil del producto fresco es un reto para la exportación. En el caso de mora de Castilla, se presentan problemas como el carácter altamente perecedero de esta fruta, con altas pérdidas poscosecha a causa de la escasa tecnología de conservación y transformación en las zonas de producción. El transporte del producto fresco es difícil, debido a que las zonas de producción generalmente están en regiones de ladera con malas vías de comunicación. Para responder a las situaciones presentadas, es necesario buscar alternativas de conservación y transformación que permitan aumentar la productividad y competitividad de los sectores mencionados. Se definieron los siguientes productos y procesos: • Mora fresca: mora refrigerada y congelada. • Mora procesada: mora en polvo obtenida por secado en bandejas. 1° ed.

Published
2022

31. Procesos agroindustriales en piña md2

Author

Díaz Ortiz, Alberto, Ochoa Martínez, Claudia Isabel, Vélez Pasos, Carlos, Ayala Aponte, Alfredo Adolfo, Fernández Quintero, Alejandro, Delgado Solarte, Diana María, Plaza Cortés, Cindy Camila, Terán Llorente, Diana Milena, González Torres, Vanessa, González Hoyos, Lina Vanessa, Benítez Franco, Juan Pablo, Vargas Serna, Claudia Liliana, Escobar Espina, Paula Andrea, Castellanos Espinosa, Diego Alberto, Moncayo Martínez, Diana Cristina, Cortés Jaimes, Lesley Andrea, Rada Bula, Adriana Isabel, Barón Cangrejo, Omar Giovanny, Posada Rubiano, Nestor Camilo, Universidad del Valle, Parque BioPacífico, Centro Internacional de Agricultura Tropical - CIAT, Universidad Nacional de Colombia, Bioversity International, Sistema General de Regalías - SGR, and Gobernación del Valle del Cauca

Subjects
Ananás comosus, Fruit industry, Agroindustria, Investigaciones, Piñas, Piña, Colombia, Agroindustry, Valle del Cauca, Proyectos, Fruticultura, Tecnología postcosecha, Agricultura de montaña, Agro-industry, Industria frutícola, Vigilancia tecnológica, Comercialización de productos agrícolas, Comercialización, Agricultural product marketing
Abstract

Ilustraciones (principalmente a color), diagramas, figuras, fotografías, En este documento se incluyen los resultados de los productos y los procesos seleccionados de acuerdo con las brechas identificadas para piña md2, tanto en fresco como procesada, y se realiza un análisis comparativo de dichos resultados. La vigilancia tecnológica se llevó a cabo incluyendo 4 tipos de vigilancias (competitiva, comercial, científico-tecnológica y estratégica). Con base en estas, se seleccionaron los productos y procesos correspondientes a la fruta que fue objeto de este estudio. (Texto tomado de la fuente) 1° ed.

Published
2022

32. Procesos agroindustriales en aguacate cv. Hass

Author

Castellanos Espinosa, Diego Alberto, Moncayo Martínez, Diana Cristina, Rada Bula, Adriana Isabel, Rodríguez Dionicio, Adriana Lisbeth, Lopera Sepúlveda, Alex Arbey, Ramírez Gil, Joaquín Guillermo, Díaz Ortiz, Alberto, Ochoa Martínez, Claudia Isabel, Vélez Pasos, Carlos, Ayala Aponte, Alfredo Adolfo, Fernández Quintero, Alejandro, Delgado Solarte, Diana María, Plaza Cortés, Cindy Camila, Terán Llorente, Diana Milena, González Torres, Vanessa, González Hoyos, Lina Vanessa, Benítez Franco, Juan Pablo, Vargas Serna, Claudia Liliana, Escobar Espina, Paula Andrea, Universidad del Valle, Parque BioPacífico, Centro Internacional de Agricultura Tropical - CIAT, Bioversity International, Sistema General de Regalías - SGR, Gobernación del Valle del Cauca, and Universidad Nacional de Colombia

Subjects
Aguacate, Fruit growing, Commercialization, Agroindustria, Colombia, Agroindustry, Valle del Cauca, Proyectos, 664 - Tecnología de alimentos, Fruticultura, Tecnología postcosecha, Postharvest Technology, Mountain farming, Agricultura de montaña, Persea americana, Agro-industry, Vigilancia tecnológica, Comercialización, Avocados
Abstract

Ilustraciones (principalmente a color), diagramas, figuras, fotografías, mapas Este documento se presenta en el marco del proyecto “Incremento de la competitividad sostenible en la agricultura de ladera en todo el departamento del Valle del Cauca, Occidente”, ejecutado por la Universidad Nacional de Colombia, sede Palmira, la Universidad del Valle y el Centro Internacional de Agricultura Tropical (Ciat), con el apoyo económico del Sistema General de Regalías de Colombia (sgr). Este estudio se desarrolló mediante la metodología de vigilancia tecnológica e inteligencia competitiva con el fin de establecer una herramienta que brinde información estratégica para la toma de decisiones de los actores involucrados en el proyecto. Su objetivo principal es identificar a través de la información recopilada, procesada y analizada los productos para desarrollar, tanto frescos como procesados, y la selección de procesos para el aguacate Hass. (Texto tomado de la fuente) 1° ed.

Published
2022

36. Modelling change in color and firmness of baby banana (Musa acuminata AA) in modified atmosphere packaging

Author

Castellanos Espinosa Diego and Algecira Enciso Néstor

Subjects
modelo matemático, respiración, firmeza de pulpa, color de corteza., Plant ecology, QK900-989
Abstract

To determine the change in the ripening stage and quality through associated variables such as firmness and peel color is a useful tool for predicting the behavior and involvement of the product stored at different changing conditions. The change in O2 and CO2 concentration, pulp firmness and peel color were measured in a test of modified atmosphere packaging for baby banana to develop a mathematical model to represent the change in firmness and color as a function of temperature, mixture of gas and time. The fruits were packaged at three temperatures (11, 13 and 17°C) and a range of combinations of steady state concentrations of modified atmospheres (5.3 to 15.7 kPa O2 and 0 to 11.0 kPa for CO2), local atmospheric pressure of 74.9 kPa (0.74 atm) and 80 % relative humidity constant in polyethylene bags (HDPE) micro-perforated for a period of 30 days. The model considers the product respiration rate by an Michaelis-Menten equation of noncompetitive inhibition, and takes into account the transfer of gases through packaging film and through micro-perforations of this; the change of firmness is considered through a first-order model, and depending on the concentration of both O2 and CO2 by Michaelis-Menten equations; color change (in Hunter Lab coordinates) was considered separately for each color coordinate, representing the coordinate L* using an increase-logistic model, a* by a zero-order model and b* by first-order model, all temperaturedependent by Arrhenius functions. The predictive capability of the developed model is appropriate, explaining 97.0% of the modified atmosphere effect on the loss of firmness, 90.6% of the change in the coordinate L*, 88.9% of the change in a* and 96.7 % of the change in b*.

Published
2012

38. 22 Un álbum inédito de Rafael Garzón sobre la Alhambra en Daimiel

Author

Clemente Espinosa, Diego and Celis Pozuelo, Alberto

Subjects
San Pedro de Galatino, Conde de Benalúa, Rafael Garzón Rodríguez, Alhambra, Granada, Álbum, Zacatena, Láchar, Daimiel, Fotografía, Photograph
Abstract

El objeto de la presente comunicación consiste en dar a conocer la existencia de un álbum de fotografías de la Alhambra realizado por el fotógrafo Rafael Garzón. La existencia del álbum reviste sumo interés puesto que éste ha permanecido inédito en una finca del ducado de San Carlos hasta fecha reciente. También es de destacar el buen estado de conservación del mismo, ya que conserva la estructura original, incluidas las tapas de piel de cocodrilo y las guardas. Éstas fueron utilizadas a modo de libro de firmas por distintas personalidades invitadas a una serie de cacerías organizadas en Granada y que han permitido establecer las coordenadas espaciotemporales del documento (¿dónde?, ¿cuándo?). El álbum se compone de 22 hojas de cartón que contienen 16 albúminas de gran formato 55.4 x 43.5 cm que nos permiten conocer la Alhambra a finales del siglo XIX. Como valor añadido, las firmas que aparecen en el álbum son un rico legado histórico para conocer la sociedad de la época, así como las primeras iniciativas turísticas alrededor del que es uno de los monumentos más importantes de España y vanguardia de esta actividad económica hasta nuestros días., The purpose of the present communication is to know the existence of an album of photographs of the Alhambra made by the photographer Rafael Garzón. The reason why the document is of great interest, is because it has been remained unpub lished in a farm of Daimiel until nowadays. It is also worth noting the good state of conservation of the same, since it retains the original structure, including crocodile skin covers and the flyleaf. These were used as a book of signatures by different personalities invited to a series of hunts organized in Granada and that allowed to establish the space-time coordinates of the document (where?, when?). The album consists of 22 sheets of cardboard containing 16 large format 55.4 x 43.5 cm albumens that allow us to know the Alhambra at the end of the XIX century. As an added value, the signatures that appear in the album are a rich historical legacy to know the society of the time, as well as the first tourist initiatives around which is one of the most important monuments of Spain and vanguard of this economic activity up to our days.

Published
2021

40. Novel CRISPR-Associated Gene-Editing Systems Discovered in Metagenomic Samples Enable Efficient and Specific Genome Engineering for Cell Therapy Development

Author

Cost, Gregory J., primary, Lamothe, Rebecca C., additional, Storlie, Meghan D., additional, Rivas, Andres, additional, Espinosa, Diego A., additional, Goltsman, Daniela S. A., additional, Alexander, Lisa, additional, Butterfield, Cristina N., additional, Brown, Christopher T., additional, and Thomas, Brian C., additional

Published
2021
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41. Multisystem Inflammatory Syndrome in Children (MIS-C) temporally related to COVID-19: the experience at a pediatric reference hospital in Colombia.

Author

Alejandro Lozano-Espinosa, Diego, Camacho-Moreno, Germán, Francisco López-Cubillos, Juan, Soraya Díaz-Maldonado, Adriana, Javier León-Guerra, Oscar, Mauricio Galvis-Trujillo, Diego, Sanguino-Lobo, Roy, Guillermo Arévalo-Leal, Oscar, María Eraso-Díaz del Castillo, Ana, Fernanda Reina-Ávila, María, Carolina Cárdenas-Hernández, Vicky, Ivankovich-Escoto, Gabriela, Tremoulet, Adriana H., and Ulloa-Gutiérrez, Rolando

Subjects
*MULTISYSTEM inflammatory syndrome in children, *CHILDREN'S hospitals, *MACROPHAGE activation syndrome, *MULTISYSTEM inflammatory syndrome, *VENTRICULAR dysfunction, *MUCOCUTANEOUS lymph node syndrome, *INTENSIVE care units
Abstract

Objective: This study aimed to describe the clinical characteristics and the different phenotypes of children with multisystem inflammatory syndrome in children (MIS-C) temporally related to COVID-19 and to evaluate the risk conditions that favored a greater severity of the disease during a 12-month period at a pediatric reference hospital in Colombia. Methods: A 12-month retrospective observational study of children under the age of 18 years who met criteria for MIS-C. Results: A total of 28 children presented MIS-C criteria. The median age was 7 years. Other than fever (100%) (onset 4 days prior to admission), the most frequent clinical features were gastrointestinal (86%) and mucocutaneous (61%). Notably, 14 (50%) children had Kawasaki-like symptoms. The most frequent echocardiographic abnormalities were pericardial effusion (64%), valvular involvement (68%), ventricular dysfunction (39%), and coronary artery abnormalities (29%). In addition, 75% had lymphopenia. All had at least one abnormal coagulation test. Most received intravenous immunoglobulin (89%), glucocorticoids (82%), vasopressors (54%), and antibiotics (64%). Notably, 61% had a more severe form of the disease and were admitted to an intensive care unit (median 4 days, mean 6 days); the severity predictors were patients with the inflammatory/MIS-C phenotype (OR 26.5; 95%CI 1.40–503.7; p=0.029) and rash (OR 14.7; 95%CI 1.2–178.7; p=0.034). Two patients had macrophage activation syndrome. Conclusions: Coronary artery abnormalities, ventricular dysfunction, and intensive care unit admission were frequent, which needs to highlight the importance of early clinical suspicion. [ABSTRACT FROM AUTHOR]

Published
2023
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43. Restauración y ampliación del palacio de la cultura y las artes de la Amazonia – Curiplaya

Author

Barbosa Páez, Juan Antonio / Director, Blain López, William / Seminarista, Garavito Almonacid, Diana Patricia / Seminarista, Coronado Cabrera, María Camila / Seminarista, Cuellar Espinosa, Diego Alejandro, Barbosa Páez, Juan Antonio / Director, Blain López, William / Seminarista, Garavito Almonacid, Diana Patricia / Seminarista, Coronado Cabrera, María Camila / Seminarista, and Cuellar Espinosa, Diego Alejandro

Abstract

Este trabajo trata sobre diseño, patrimonio y luz. Tiene como objetivo recuperar un patrimonio arquitectónico que permita la percepción continua de espacios mediante la luz y conexión simbólica de los hitos en el entorno inmediato, generando sitios adecuados para la divulgación y difusión de la cultura. Para ello fue necesario: identificar un contexto urbano donde se requiere la recuperación y ampliación de un patrimonio, realizar una revisión de autores y referentes para involucrar desde la arquitectura la memoria colectiva, visual y la luz, por ultimo aplicar las estrategias y operaciones de diseño en el patrimonio promoviendo la conexión simbólica e integrando a las dinámicas urbanas. Por lo tanto, durante el desarrollo del proyecto, se puede inferir que, se generaron las determinantes de diseño del proyecto, logrando una integración adecuada respecto a su entorno, respetando los valores estéticos del lugar de implantación, configurándolo hacia el paisaje patrimonial y conexión con el entorno generando espacios adecuados para la divulgación de la cultura., This work is about design, heritage and light. Its objective is to recover an architectural heritage that allows the continuous perception of spaces through light and symbolic connection of landmarks in the immediate environment, generating suitable sites for the dissemination and dissemination of culture. For this, it was necessary: to identify an urban context where the recovery and expansion of a heritage is required, to carry out a review of authors and references to involve the collective memory, visual and light from the architecture, finally to apply the strategies and design operations in heritage promoting symbolic connection and integrating urban dynamics. Therefore, during the development of the project, it can be inferred that the design determinants of the project were generated, achieving an adequate integration with respect to its environment, respecting the aesthetic values of the place of implantation, configuring it towards the heritage landscape and connection with the environment

Published
2021

46. Caracterización de pacientes con dislipidemia en un hospital regional de Colombia

Author

González Soriano, Luz Stella, Lozano Espinosa, Diego Alejandro, Bornachero Pinto, Diego Armando, González Soriano, Luz Stella, Lozano Espinosa, Diego Alejandro, and Bornachero Pinto, Diego Armando

Abstract

La dislipidemia constituye un factor de riesgo mayor y modificable en la génesis de las enfermedades cardiovasculares. Objetivo: Caracterizar los pacientes adultos con dislipidemia que consultan al servicio ambulatorio del Hospital Regional Valle de Tenza, Guateque Boyacá, Colombia, en el año 2014. Material y Método: Se realizó un estudio observacional de corte trasversal retrospectivo. Se incluyeron los pacientes de 18 o más años con alteraciones en el perfil lipídico en seguimiento por consulta ambulatoria del Hospital Regional Valle de Tenza, Guateque Boyacá, Colombia en el 2014, utilizando información registrada en resultados de laboratorio e historias clínicas, análisis efectuado mediante el programa EPI INFO versión 7.

Published
2020

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