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Presenteeism is defined as the presence of the worker at their workplace despite not being in optimal physical or mental conditions. Presenteeism is a phenomenon that has been poorly studied in the context of healthcare. Despite the many negative consequences associated with presenteeism, to date, no studies have investigated this issue in nurses in Spain. The objective was to develop and validate a questionnaire on presenteeism to be used by nursing staff in Spain. Methods: A psychometric study for the development and validation of a questionnaire. The PRESENCA® questionnaire on presenteeism was created by a panel of experts, based on a survey comprised of 31 Likert-type items. Results: In total, 355 nurses completed the questionnaire. The factorial analysis revealed the existence of 3 factors and confirmed appropriate levels of validity and reliability (alpha = 0.729). Conclusions: The PRESENCA® questionnaire is the first tool developed and validated in Spanish for the assessment of presenteeism in nursing. Our findings demonstrate that this scale has appropriate psychometric properties and its use may facilitate the detection of presenteeism among professionals. As a result, use of this questionnaire may contribute towards the improvement of clinical safety.

The voltage-dependent potassium channel Kv1.3 has been implicated in proliferation in many cell types, based on the observation that Kv1.3 blockers inhibited proliferation. By modulating membrane potential, cell volume, and/or Ca2+ influx, K+ channels can influence cell cycle progression. Also, noncanonical channel functions could contribute to modulate cell proliferation independent of K+ efflux. The specificity of the requirement of Kv1.3 channels for proliferation suggests the involvement of molecule-specific interactions, but the underlying mechanisms are poorly identified. Heterologous expression of Kv1.3 channels in HEK cells has been shown to increase proliferation independently of K+ fluxes. Likewise, some of the molecular determinants of Kv1.3-induced proliferation have been located in the C-terminus region, where individual point mutations of putative phosphorylation sites (Y447A and S459A) abolished Kv1.3-induced proliferation. Here, we investigated the mechanisms linking Kv1.3 channels to proliferation exploring the correlation between Kv1.3 voltage-dependent molecular dynamics and cell cycle progression. Using transfected HEK cells, we analyzed both the effect of changes in resting membrane potential on Kv1.3-induced proliferation and the effect of mutated Kv1.3 channels with altered voltage dependence of gating. We conclude that voltage-dependent transitions of Kv1.3 channels enable the activation of proliferative pathways. We also found that Kv1.3 associated with IQGAP3, a scaffold protein involved in proliferation, and that membrane depolarization facilitates their interaction. The functional contribution of Kv1.3-IQGAP3 interplay to cell proliferation was demonstrated both in HEK cells and in vascular smooth muscle cells. Our data indicate that voltage-dependent conformational changes of Kv1.3 are an essential element in Kv1.3-induced proliferation., Secretaría de Estado de Investigación,Desarrollo e Innovación,Grant/Award Numbers: BFU2016‐75360‐R,SEV‐2015‐0505; Consejería de Educación,Junta de Castilla y León,Grant/Award Number: VA114P17

Producción Científica, Changes in voltage-dependent potassium channels (Kv channels) associate to proliferation in many cell types, including transfected HEK293 cells. In this system Kv1.5 overexpression decreases proliferation, whereas Kv1.3 expression increases it independently of K+ fluxes. To identify Kv1.3 domains involved in a proliferation-associated signaling mechanism(s), we constructed chimeric Kv1.3-Kv1.5 channels and point-mutant Kv1.3 channels, which were expressed as GFP- or cherry-fusion proteins. We studied their trafficking and functional expression, combining immunocytochemical and electrophysiological methods, and their impact on cell proliferation. We found that the C terminus is necessary for Kv1.3-induced proliferation. We distinguished two residues (Tyr-447 and Ser-459) whose mutation to alanine abolished proliferation. The insertion into Kv1.5 of a sequence comprising these two residues increased proliferation rate. Moreover, Kv1.3 voltage-dependent transitions from closed to open conformation induced MEK-ERK1/2-dependent Tyr-447 phosphorylation. We conclude that the mechanisms for Kv1.3-induced proliferation involve the accessibility of key docking sites at the C terminus. For one of these sites (Tyr-447) we demonstrated the contribution of MEK/ERK-dependent phosphorylation, which is regulated by voltage-induced conformational changes., Ministerio de Economía y Competitividad (MINECO), Instituto de Salud Carlos III y Programa Estatal de Investigación , Fundación Ramón Areces y Consejería de Sanidad de la Junta de Castilla y León.

This methodological paper discusses how photographs can be used in multi-layered data projects with children and families. We present photographs as a versatile low-fi digital artifact that can be used under a variety of research circumstances and critically discuss this particular visual tool in the context of the growing body of visual and multimodal research with children and families. The critical discussion draws on a series of research projects in which we have employed photographs (topics of the projects include family diversity or children's routines). The comparisons between projects highlights some of the procedural and analytical choices that are opened up when using photographs. In particular, we focus on two issues: (a) differences that emerge when materials are created by participants or are elicited by researchers, and; (b) the metaphors that are applied to interpret and work with photographs, En este artículo metodológico analizamos cómo las fotografías pueden ser utilizadas en proyectos de investigación con infancia y familias que usan diferentes clases de datos. Planteamos las fotografías como un artefacto digital the "baja fidelidad" ("low-fi") versátil que puede utilizarse bajo una variedad de condiciones de investigación y discutimos críticamente esta herramienta visual, particularmente en el contexto de la creciente investigación visual y multimodal con infancia y familias. La discusión crítica se basa en una serie de proyectos de investigación en los que hemos empleado fotografías (los temas de los proyectos incluyen diversidad familiar o rutinas de la infancia). La comparación entre los proyectos sirve para resaltar algunas de las opciones analíticas y procedimentales que se abren cuando se usan fotografías. Específicamente, nos centramos en dos cuestiones: (a) las diferencias que surgen cuando los materiales fotográficos son creados por los participantes o son provocados por la investigación y; (b) las metáforas que se aplican para interpretar y trabajar con fotografías

espanolEl objetivo principal de este trabajo sera elaborar un cuestionario que nos permita conocer la percepcion de los trabajadores sobre las actividades que realiza la enfermeria del trabajo en los diferentes Servicios de Prevencion. Metodo: El cuestionario se divide en tres partes: 1) Variables sociodemograficas, formadas por 4 items; 2) Opinion sobre el servicio de prevencion, formado por 15 items; 3) Opinion de los trabajadores sobre la atencion de enfermeria, formado por 23 items. Se analizo el comportamiento metrico del cuestionario respecto a la validez de constructo, de criterio y la consistencia interna, para lo cual, se opta por hacerlo segun los criterios basicos de Moriyama y el calculo de indice ∂ de Conbrach. Para ello se repartieron 55 cuestionarios de control en los diferentes servicios de prevencion de la Comunidad de Cantabria y se trataron los datos con el programa SPSS v.15 de analisis estadistico. Resultados: Se supero el criterio de validacion establecido, con resultados superiores al 80% en la valoracion de los expertos. Encontramos una buena consistencia interna, evidenciada por un Alfa de Cronbach de 0.837 en cada seccion. Conclusion: El cuestionario reune suficientes propiedades psicometricas para ser considerado una herramienta util y fiable capaz de medir con objetividad la satisfaccion de los trabajadores con los cuidados de la enfermeria del trabajo. EnglishThe main aim of this study is to draw up a "Questionnaire on Satisfaction with Occupational Health Nursing" (Cuestionario de satisfaccion con los cuidados de la enfermeria del trabajo - CUSACET), which will enable us to gauge users' perceptions with regard to the occupational health nursing work carried out by the various safety management services. Materials and Method: The questionnaire is designed to encompass three dimensions: a. Sociodemographic: consisting of four items. b. Opinion of the safety management services: consisting of fifteen items. c. Opinion of care received from the nursing staff at the safety management service, measured by twenty-three items. We opted to do the validation according to the basic criteria of Moriyama and by applying Cronbach's Alpha index. To this end, 55 control questionnaires were given out at various safety management services in Cantabria. The questionnaires were collected and the data recorded onto the statistical analysis program SPSS v.15. Results: The criterion established for validity was exceeded, with results above 80% in assessment by experts in accordance with Moriyama's basic criteria. We encountered good internal consistency, as evidenced by a Cronbach Alpha coefficient of 0.837 in each section. Conclusions: This paper presents a new instrument, specifically designed to measure the quality of occupational health nursing care through the perceptions of the users who receive it which is reliable and easy to use.

En este artículo se pretende analizar un sesgo de la relación que existe entre la demanda de perfección que recae sobre elsujeto contemporáneo y el individualismo resultante, mediante conceptos del psicoanálisis para precisar algunos de sus efectos. Partimos del supuesto de que como consecuencia de los imperativos sociales que se despliegan en la actualidad a manera de demanda y exigencia, los sujetos producen un engaño imaginario cuya trama proyecta la ilusión de que es posible lograr la completud o perfección, lo cual implicaría en esencia la no necesidad del otro, donde cada uno lo es por sí mismo, derivándose el individualismo que caracteriza a la presente época.

Vascular smooth muscle cells (VSMCs) switch from a contractile to a proliferative phenotype, in response to arterial injury, such as after percutaneous coronary interventions (PCI). This phenotypic switch implies a profound change in the expression of contractile proteins and ion channels. In a

Phenotypic modulation (PM) of vascular smooth muscle cells (VSMCs) is central to the process of intimal hyperplasia which constitutes a common pathological lesion in occlusive vascular diseases. Changes in the functional expression of Kv1.5 and Kv1.3 currents upon PM in mice VSMCs have been found to contribute to cell migration and proliferation. Using human VSMCs from vessels in which unwanted remodeling is a relevant clinical complication, we explored the contribution of the Kv1.5 to Kv1.3 switch to PM. Changes in the expression and the functional contribution of Kv1.3 and Kv1.5 channels were studied in contractile and proliferating VSMCs obtained from human donors. Both a Kv1.5 to Kv1.3 switch upon PM and an anti-proliferative effect of Kv1.3 blockers on PDGF-induced proliferation were observed in all vascular beds studied. When investigating the signaling pathways modulated by the blockade of Kv1.3 channels, we found that anti-proliferative effects of Kv1.3 blockers on human coronary artery VSMCs were occluded by selective inhibition of MEK/ERK and PLCγ signaling pathways, but were unaffected upon blockade of PI3K/mTOR pathway. The temporal course of the anti-proliferative effects of Kv1.3 blockers indicates that they have a role in the late signaling events essential for the mitogenic response to growth factors. These findings establish the involvement of Kv1.3 channels in the PM of human VSMCs. Moreover, as current therapies to prevent restenosis rely on mTOR blockers, our results provide the basis for the development of novel, more specific therapies., Supported by grants from the Ministerio de Economía y Competitividad, Instituto de Salud Carlos III (RIC RD12/0042/0006, Red Heracles), Ministerio de Ciencia e Innovación (BFU2010-15898 to MTPG), Junta de Castilla y León (VA094A11-2 to, JRLL), and Fondo de Investigaciones Sanitarias (FIS PI11/00225 to MR)

El pdf del trabajo es la versión post-print., Chemoreceptor cells from rabbit carotid body (CB) exhibit transient outward currents reversibly inhibited by low P(o2). Molecular and functional dissection of the components of these outward currents indicates that at least two different channels (Kv4.3 and Kv3.4) contribute to this current. Furthermore, several lines of evidence support the conclusion that Kv4 channel subfamily members (either Kv4.3 alone or Kv4.3/Kv4.1 heteromultimers) are the oxygen sensitive K channels (K(o2)) in rabbit CB chemoreceptor cells. However, the pharmacological characterization of these currents shows that they are almost completely blocked by high external TEA concentrations, while Kv4 channels have been shown to be TEA-insensitive. We hypothesized that the expression of regulatory subunits in chemoreceptor cells could modify TEA sensitivity of Kv4 channels. Here, we explore the presence and functional contribution of DPPX to K(o2) currents in rabbit CB chemoreceptor cells by using DPPX functional knockdown with siRNA. Our data suggest that DPPX proteins are integral components of K(o2) currents, and that their association with Kv4 subunits modulate the pharmacological profile of the heteromultimers., This work was supported by Ministerio de Sanidad y Consumo, Instituto de Salud Carlos III grants R006/009 (Red Heracles) and PI041044 (JRLL), Ministerio de Educación y Ciencia grant BFU2004-05551 (MTPG) and Junta de Castilla y León grant VA011C05.

The plasticity of vascular smooth muscle cells that can switch from a contractile to a proliferative phenotype associates with several cardiovascular diseases. We have previously demonstrated that changes in the expression of voltage-dependent potassium (Kv) channels associated with this proliferative phenotype. These results were validated in transfected HEK293 cells: Kv1.5 decreased proliferation and Kv1.3 increased cell proliferation independently of ion flux (Cidad et al. ATVB 2012, 32:1299-307). Our data also suggested that the movement of the voltage-sensor of Kv1.3 channels could be coupled to signaling pathways leading to proliferation.Here we explore Kv1.3 regions involved in the signaling mechanism(s) associated with proliferation, by constructing chimeric Kv1.3-Kv1.5 channels and point-mutant Kv1.3 channels without consensus phosphorylation sites. These constructs were expressed as GFP- or cherry-fusion proteins, and we studied their functional expression, their effects on proliferation and their phosphorylation state in HEK cells combining immunocytochemical, electrophysiological and EdU incorporation studies.The molecular determinants of Kv1.3-induced proliferation are located at the carboxy-terminal domain. We identified two close residues (Y447 and S459) whose mutation abolished proliferation. Moreover, proliferation was increased with chimeric Kv1.5 mutants containing this segment (YS segment) but only when located at specific place within the channel molecule. Besides the selective MEK/ERK blocker PD98059 was able to inhibit Kv1.3-induced proliferation and tyrosine phosphorylation of the channel, having no effect on Y447A mutant channel. Finally, voltage-dependent transitions of Kv1.3 from close to open conformation increased pTyr labelling.We conclude that the signaling pathway linking Kv1.3 channels with proliferation involves voltage-induced conformational changes promoting tyrosine phosphorylation. Both phosphorylation and proliferation increase by facilitating the closed to open transition, being diminished in mutant channels that reside in the inactivated state. Supported by ISCIII-FEDER RD12/0042/0006, and BFU2013-45867-R grants.

Resumen del poster presentado al American College of Cardiology - 61st Annual Scientific Seesion & Expo celebrado en Chicago (US) del 24 al 27 de marzo de 2012.