Search

Your search keyword '"Escobar-Curbelo, Luis"' showing total 10 results
Start Over Author "Escobar-Curbelo, Luis"
10 results on '"Escobar-Curbelo, Luis"'

5. Insulin degrading enzyme is up-regulated in pancreatic β cells by insulin treatment

Author

Fernández-Díaz, C., Escobar-Curbelo, Luis, López-Acosta, José Francisco, Lobatón, Carmen D., Moreno, Alfredo, Sanz-Ortega, Julián, Perdomo, Germán, Cózar-Castellano, Irene, Ministerio de Economía y Competitividad (España), and European Commission

Subjects
Human islets, Beta-cells, OHAs, 6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncología [CDU], Beta cells, Type 2 diabetes, Rodent islets, Alpha-cells, Endocrinology, Insulin treatment, Endocrinología, Insulin-degrading enzyme
Abstract

Insulin Degrading Enzyme (IDE) is an endopeptidase that degrades insulin and glucagon. Ide gene has been associated with type-2 diabetes mellitus (DM2). However, the physiological role(s) of IDE in glucose homeostasis and its potential therapeutic benefit remain not completely known. To contribute in the understanding of IDE's role in glucose metabolism, we analyzed IDE protein level in pancreatic islets from two hyperinsulinemic mouse models, db/db and high-fat diet (HFD) mice, as well as in human islets from DM2 patients treated with oral hypoglycemic agents (OHAs) or insulin. IDE protein level was detected by staining and by western-blot. INS1E cells, rat and human islets were treated with insulin and IDE protein level was studied. We have shown for the first time IDE staining in rodent and human tissue, using the proper negative control, IDE null mouse tissue. Our staining indicates that IDE is expressed in both beta- and alpha-cells, with higher expression in alpha-cells. Db/db and HFD mice islets showed increased IDE protein level. Interestingly, human islets from DM2 patients treated with OHAs showed decreased IDE protein level in beta-cells. Meanwhile, islets from insulin-treated DM2 patients showed augmented IDE protein level compared to OHAs patients, pointing to an upregulation of IDE protein level stimulated by insulin. These data correlate nicely with insulin-stimulated upregulation of IDE in cultured INS1E cells, as well as in rat and human islets. In conclusion, our study shows that IDE is expressed in pancreatic beta- and alpha-cells of both rodents and humans, having higher expression in alpha-cells. Furthermore, insulin stimulates IDE protein level in pancreatic beta-cells. These results may have implications in how DM2 patient's treatment affects their beta-cell function., This research has been funded by Ministerio de Economia y Competitividad-Government of Spain and FEDER (SAF2014-58702-C2-1-R and SAF2014-58702-C2-2-R) to IC and to GP respectively.

Published
2018

8. Colaboradores

Author

Abad Manteca, Laura, Aguado García, José M., Alcalá Díaz, Juan F., Aller Fernández, Marta, Álvarez Escudero, Rocío, Armiñanzas Castillo, Carlos, Arnaiz de las Revillas Almajano, Francisco, García de la Santa, Jaime Atance, Baamonde Calzada, Carmela, Benítez Toledo, María J., Berciano Blanco, José A., Berrazueta Fernández, José R., Blanco Alonso, Ricardo, Burón Mediavilla, Francisco J., Cabezas Olea, Rita, Calvo Manuel, Elpidio, Crespo Facorro, Benedicto, Crespo García, Javier, Crespo del Pozo, Juan, Cuadrado Medina, Francisca, Delgado Lista, Francisco J., Delgado Alvarado, Manuel, Escobar Curbelo, Luis, Fariñas Álvarez, María C., Fernández Fresnedo, Gema, Fernández Sampedro, Marta, Fernández de la Puebla Giménez, Rafael A., Fuentes Jiménez, Francisco J., Gallardo Agromayor, Elena, García Ibarbia, Carmen, García Klepzig, José L., García Unzueta, Mayte, García Hoyos, Marta, Garmilla Ezquerra, Pablo, Gómez Garduño, Ángela, Gómez Delgado, Francisco, González-Mandly, Andrés, González Sánchez, Francisco J., González Vílchez, Francisco J., González Macías, Jesús, González Rozas, Marta, González-Gay, Miguel A., Hernández Hernández, José L., Ibáñez Botella, Alba, Iguarán Bermúdez, Rosario, Infante Ceberio, Jon, Jaén-Águila, Fernando, Jáimez-Gámiz, Laura, Jiménez-Alonso, Juan F., Jiménez López, M. Ángeles, Landeras Álvaro, Rosa, Larrosa Campo, Davinia, López Miranda, José, Manzano-Gamero, M. Victoria, Marco de Lucas, Enrique, Mario Sabio, José, Martín de Francisco Hernández, Ángel L., Martín Escudero, Juan C., Martínez Taboada, Víctor M., Mayoral van Son, Jacqueline, Morchón Simón, David, Navarrete-Navarrete, Nuria, Neila Calvo, Sara, Olmos Martínez, José M., Ortiz Flores, Fernando, Oterino Durán, Agustín, Parra Blanco, José A., Pascual Gómez, Julio, Pedraz González-Tablas, Teresa, Pérez Castrillón, José L., Pérez Martínez, Pablo, Piedra León, María, Pineda Alonso, Mónica, Polo Esteban, José M., Puente Sanchez, Ángela M., Riancho Moral, José A., Riancho Zarrabeitia, Javier, Riancho Zarrabeitia, Leyre, Rodríguez Rodríguez, Eloy M., Salas Pelayo, Cristina, Sánchez Gil, Justo, Sangil Monroy, Irene, Santurtún Zarrabeitia, Ana, Santurtún Zarrabeitia, Maite, Silva, José T., Suso Estivalez, Catalina, Trippe, Jorn, Valero Díaz Lamadrid, María C., Vargas-Hitos, José A., Vázquez de Prada, José A., Vázquez Jacinto, Pedro, Vega Tejedor, Gemma, and Zarrabeitia Cimiano, María T.

Published
2014
Full Text
View/download PDF

10. Insulin degrading enzyme is up-regulated in pancreatic β cells by insulin treatment.

Author

Fernández-Díaz CM, Escobar-Curbelo L, López-Acosta JF, Lobatón CD, Moreno A, Sanz-Ortega J, Perdomo G, and Cózar-Castellano I

Subjects
Animals, Cells, Cultured, Diabetes Mellitus, Experimental drug therapy, Diabetes Mellitus, Experimental pathology, Diabetes Mellitus, Type 2 drug therapy, Diabetes Mellitus, Type 2 pathology, Humans, Hypoglycemic Agents therapeutic use, Insulin therapeutic use, Mice, Up-Regulation, Diabetes Mellitus, Experimental metabolism, Diabetes Mellitus, Type 2 metabolism, Glucagon-Secreting Cells enzymology, Hypoglycemic Agents pharmacology, Insulin pharmacology, Insulin-Secreting Cells enzymology, Insulysin biosynthesis, Islets of Langerhans metabolism
Abstract

Insulin Degrading Enzyme (IDE) is an endopeptidase that degrades insulin and glucagon. Ide gene has been associated with type-2 diabetes mellitus (DM2). However, the physiological role(s) of IDE in glucose homeostasis and its potential therapeutic benefit remain not completely known. To contribute in the understanding of IDE's role in glucose metabolism, we analyzed IDE protein level in pancreatic islets from two hyperinsulinemic mouse models, db/db and high-fat diet (HFD) mice, as well as in human islets from DM2 patients treated with oral hypoglycemic agents (OHAs) or insulin. IDE protein level was detected by staining and by western-blot. INS1E cells, rat and human islets were treated with insulin and IDE protein level was studied. We have shown for the first time IDE staining in rodent and human tissue, using the proper negative control, IDE null mouse tissue. Our staining indicates that IDE is expressed in both beta- and alpha-cells, with higher expression in alpha-cells. Db/db and HFD mice islets showed increased IDE protein level. Interestingly, human islets from DM2 patients treated with OHAs showed decreased IDE protein level in beta-cells. Meanwhile, islets from insulin-treated DM2 patients showed augmented IDE protein level compared to OHAs patients, pointing to an upregulation of IDE protein level stimulated by insulin. These data correlate nicely with insulin-stimulated upregulation of IDE in cultured INS1E cells, as well as in rat and human islets. In conclusion, our study shows that IDE is expressed in pancreatic beta- and alpha-cells of both rodents and humans, having higher expression in alpha-cells. Furthermore, insulin stimulates IDE protein level in pancreatic beta-cells. These results may have implications in how DM2 patient's treatment affects their beta-cell function.

Published
2018
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results