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This study aimed to identify the skin temperature (Tsk) behavior to understand the acute cross-effect after unilateral training of lower-limbs. Seventeen healthy young men (weight, 75.2±5.5 kg; height, 1.8±0.1 m; age, 22.5±1.6 years) were divided into two groups: high-trained (n=8) and low-trained (n=9). All participants performed: (a) one-repetition maximum (1RM) testing protocol on the leg press, (b) a unilateral training protocol (4×10 repetitions at 70% of 1RM for leg press and 4×10 repetitions at 50% of 1RM for knee extension). Pre- and posttraining thermal images were recorded. The main results showed that independent of the limb (exercised vs. nonexercised), differences between low- and high-trained were observed for all regions of interest (ROI) except for the anterior knee: posttraining, 30-min and 60-min posttraining in nonexercised limb. The increase of contralateral Tsk was more than 50% on the ROIs corresponding to the exercises muscles 30-min post-training in low-trained but was not so high in high-trained ( P <0.05). Low-trained subjects incremented more the Tsk than high-trained in both legs after exercise. In conclusion, we observed an acute contralateral Tsk effect to unilateral training on the Tsk of the nonexercised limb, reliant on the training level of the subject., Competing Interests: CONFLICT OF INTEREST No potential conflict of interest relevant to this article was reported.