Objective: To determine the diagnostic performance of the immunochromatography technique or lateral flow for the detection of antibodies in patients with human fasciolosis., Materials and Methods: Observational, prospective and cross-sectional study. We have developed a lateral flow test (Fasciorap) for the serological diagnosis of fasciolosis due to F. hepatica, composed of excretion-secretion antigens of adult forms conjugated with orocolloid of 40 nm and a protein A and IgG of rabbit anti F. hepatica as detector reagents in the test and control line, flanked by pads in a cassette. 240 sera were evaluated, 120 positive, 50 sera from patients with other parasites, 20 from patients with infectious diseases and 50 sera from non-parasitized people, the interpretation of results was performed by visual inspection 15 minutes after applying the samples., Results: The test detected the presence of antibodies in the serum of patients with fasciolosis, reaching a sensitivity of 92.5%, a specificity of 94.17%, a positive predictive value of 94.07% and a negative predictive value of 92.62%; with 100% agreement on repeatability and reproducibility., Conclusions: Fasciorap detects cases of fascioliasis, therefore, it is a potential diagnostic test in endemic areas where point-of-care testing is required.