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9 results on '"Errico, Maria Cristina"'

6. Melanoma stem-like cells and melanoma cell lines: main molecular pathways and possible microRNA involvement

Author

Errico, Maria Cristina

Subjects
neoplasms, CSCs, melanoma, microRNAs, ETS-1
Abstract

Cancer Stem Cells (CSCs) have been identified in several malignancies and indirect evidences support the existence of melanoma stem-like cells. Since microRNAs (miRs), an abundant class of small non-coding RNAs playing important roles in regulating gene expression, are involved in melanoma development and progression, we investigated their possible involvement also in melanoma stem-like cells. We identified a subset of melanoma cells, isolated from lung metastatic melanomas that, cultured in appropriate serum free conditions, were able to propagate as non adherent spheres (SC), whereas in the presence of serum they adhere to the plastic and acquire the typical morphologic features of differentiated cells (PC). These melanoma spheres are also capable of self-renewing and tumorigenesis in vivo. Here we analyzed a group of selected miRs by qReal Time PCR and among them miR-221/-222 had the most consistent and significant increased expression in PC cells. In these cells we confirmed the inverse correlation between microRNA-221/-222 and p27Kip1 and c-KIT receptor, already reported in melanoma development and progression. Moreover, looking for new miR-221/-222-dependent target genes, we selected the proto-oncogene ETS-1 because its functional role in melanoma development was not completely elucidated. In fact ETS-1 has been reported either as a valuable diagnostic/prognostic marker or as molecule without a clear association with clinical outcome. Here we demonstrated that in melanoma stem cells as well as in melanoma cell lines ETS-1 is a direct target of miR-222. Furthermore we showed the existence of an ETS1-mediated repression of miR-222 in melanoma cell lines, thus we suggest that in putative melanoma stem cells proliferating as spheres (Mel SC) in comparison to their adherent counterpart (Mel PC) may be active an ETS-1 miR-222 circuitry whose deregulation could be relevant for melanoma progression. Further studies are in progress to understand the function of miRNAs in the context of gene networks controlling cell differentiation and tumorigenicity in melanoma stem-like cells.

Published
2011

8. RANDOMIZED TRIAL ON HYPOTHERMIC OXYGENATED PERFUSION VS STATIC COLD STORAGE IN LIVER TRANSPLANTATION FROM EXTENDED CRITERIA DONORS: INTERIM ANALYSIS

Author

Matteo Ravaioli, Giuliana Germinario, Lorenzo Maroni, Federica Odaldi, Guido Fallani, Enrico Prosperi, Chiara ZanfI, Valentina Rosa Bertuzzo, Massimo Del Gaudio, Matteo Serenari, Andrea Laurenzi, Francesco Vasuri, Gabriela Sangiorgi, Antonietta D’errico, Maria Cristina Morelli, Matteo Cescon, and Matteo Ravaioli, Giuliana Germinario, Lorenzo Maroni, Federica Odaldi, Guido Fallani, Enrico Prosperi, Chiara ZanfI, Valentina Rosa Bertuzzo, Massimo Del Gaudio, Matteo Serenari, Andrea Laurenzi, Francesco Vasuri, Gabriela Sangiorgi, Antonietta D’errico, Maria Cristina Morelli, Matteo Cescon

Subjects
HYPOTHERMIC OXYGENATED PERFUSION STATIC COLD STORAGE LIVER TRANSPLANTATION EXTENDED CRITERIA DONORS
Published
2021

9. [The biological resources and molecule archives organization: turn a need into an opportunity for the Smart Specialization Strategy of the Lazio region.]

Author

Bravo E, Franchin T, Santoro F, Conti L, Carrara S, Marino M, Di Caro A, Cigliana G, Mandoj C, Baselice S, Daniele N, Giampaoli S, Quintarelli C, Salerno M, Zinno F, Russo G, Errico MC, Minghetti L, Ricciardi W, and Napolitano M

Subjects
Biological Specimen Banks standards, Biomedical Research standards, Biotechnology standards, Humans, Italy, Specimen Handling standards, Biological Specimen Banks organization & administration, Biomedical Research organization & administration, Biotechnology organization & administration
Abstract

Smart Specialization Strategy (S3) of Lazio defines smart specialization strategies to bring out the excellence of the territory with prospects of success on the global market. Chemical-pharmaceutical, biomedical and biotechnological field is one of the 7 sectors considered of greatest interest for the S3. Key engine of biotechnology development are biological materials and associated data, stored in biobanks. However, to ensure that the research and product development carried out with that resources gives statistically significant and reproducible results, it is essential that they are collected, manipulated and stored using standardized and traced methods. Implementation of the recent published standard ISO 20387- "Biotechnology-Biobanking-General requirements for biobanking" is bridging biobanks toward to storage and distribution of qualified biological material only. Human biobanks are also an essential part of the assistance and care of the citizen and constitute an unavoidable cost of the regional health system. However, biobanks organization, rationalization of their territorial distribution, completion of the process of recognition and regional accreditation, parallel to the implementation of the offer of remunerated services for biobanking, can turn the cost of the necessary preservation of the samples, into an opportunity of territorial development. The paper describes the necessity, shared by a working group represented by several Lazio biobanks, of including biobank activities in the virtuous circle designed by the S3,concretizing the framework prefigured by the S3 document on infrastructures for research, innovation and technology transfer. To allow inclusion of biobank activities in the virtuous circle, we underline the need to quickly start the process of recognition of the territorial research biobanks, to implement at regional level the process of optimization and rationalization of the management of biological samples, in accordance with the international harmonization standards and with the territorial indications of sustainability.

Published
2019
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