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1,650 results on '"Errachid, A."'

9. Salivary Cortisol Detection with a Fully Inkjet-Printed Paper-Based Electrochemical Sensor

Author

Miguel Zea, Hamdi Ben Halima, Rosa Villa, Imad Abrao Nemeir, Nadia Zine, Abdelhamid Errachid, and Gemma Gabriel

Subjects
inkjet printing, paper substrate, cortisol, stress hormone, electrochemical impedance spectroscopy, electrochemical sensor, Mechanical engineering and machinery, TJ1-1570
Abstract

Electrochemical paper-based analytical devices (ePADs) offer an innovative, low-cost, and environmentally friendly approach for real-time diagnostics. In this study, we developed a functional all-inkjet paper-based electrochemical immunosensor using gold (Au) printed ink to detect salivary cortisol. Covalent binding of the cortisol monoclonal antibody onto the printed Au surface was achieved through electrodeposition of 4-carboxymethylaniline (CMA), with ethanolamine passivation to prevent non-specific binding. The ePAD exhibited a linear response within the physiological cortisol range (5–20 ng/mL), with sensitivities of 25, 23, and 19 Ω·ng/mL and R2 values of 0.995, 0.979, and 0.99, respectively. Additionally, interference studies against tumor necrosis factor-α (TNF-α) and N-terminal pro-B-type natriuretic peptide (NT-proBNP) yielded excellent results. This novel ePAD, fabricated using inkjet printing technology on paper, simplifies the process, reduces environmental impact, and lowers fabrication costs.

Published
2024
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28. Determination of Charge Transfer Resistance from Randles Circuit Spectra Using Elliptical Fitting

Author

Pfeiffer, Norman, Wachter, Toni, Frickel, Jürgen, Halima, Hamdi Ben, Hofmann, Christian, Errachid, Abdelhamid, Heuberger, Albert, Filipe, Joaquim, Editorial Board Member, Ghosh, Ashish, Editorial Board Member, Prates, Raquel Oliveira, Editorial Board Member, Zhou, Lizhu, Editorial Board Member, Gehin, Claudine, editor, Wacogne, Bruno, editor, Douplik, Alexandre, editor, Lorenz, Ronny, editor, Bracken, Bethany, editor, Pesquita, Cátia, editor, Fred, Ana, editor, and Gamboa, Hugo, editor

Published
2022
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33. An ImmunoFET Coupled with an Immunomagnetic Preconcentration Technique for the Sensitive EIS Detection of HF Biomarkers

Author

Hamdi Ben Halima, Nadia Zine, Imad Abrao Nemeir, Norman Pfeiffer, Albert Heuberger, Joan Bausells, Abdelhamid Elaissari, Nicole Jaffrezic-Renault, and Abdelhamid Errachid

Subjects
heart failure, interleukin-10, tumor necrosis factor-α, electrochemical impedance spectroscopy, saliva analysis, Mechanical engineering and machinery, TJ1-1570
Abstract

We propose a new strategy using a sandwich approach for the detection of two HF biomarkers: tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10). For this purpose, magnetic nanoparticles (MNPs) (MNPs@aminodextran) were biofunctionalized with monoclonal antibodies (mAbs) using bis (sulfosuccinimidyl) suberate (BS3) as a cross-linker for the pre-concentration of two biomarkers (TNF-α and IL-10). In addition, our ISFETs were biofunctionalized with polyclonal antibodies (pAbs) (TNF-α and IL-10). The biorecognition between pAbs immobilized on the ISFET and the pre-concentrate antigen (Ag) on MNPs was monitored using electrochemical impedance spectroscopy (EIS). Our developed ImmunoFET showed a low detection limit (0.03 pg/mL) toward our target analyte when compared to previously published electrochemical immunosensors. It showed a higher sensitivity than for other HF biomarkers. Finally, the standard addition method was used to determine the unknown concentration in artificial saliva. The results matched with the expected values well.

Published
2024
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48. Salivary Cortisol Detection with a Fully Inkjet-Printed Paper-Based Electrochemical Sensor.

Author

Zea, Miguel, Ben Halima, Hamdi, Villa, Rosa, Nemeir, Imad Abrao, Zine, Nadia, Errachid, Abdelhamid, and Gabriel, Gemma

Subjects
ELECTROCHEMICAL sensors, IMPEDANCE spectroscopy, HYDROCORTISONE, PASSIVATION, ELECTROPLATING
Abstract

Electrochemical paper-based analytical devices (ePADs) offer an innovative, low-cost, and environmentally friendly approach for real-time diagnostics. In this study, we developed a functional all-inkjet paper-based electrochemical immunosensor using gold (Au) printed ink to detect salivary cortisol. Covalent binding of the cortisol monoclonal antibody onto the printed Au surface was achieved through electrodeposition of 4-carboxymethylaniline (CMA), with ethanolamine passivation to prevent non-specific binding. The ePAD exhibited a linear response within the physiological cortisol range (5–20 ng/mL), with sensitivities of 25, 23, and 19 Ω·ng/mL and R2 values of 0.995, 0.979, and 0.99, respectively. Additionally, interference studies against tumor necrosis factor-α (TNF-α) and N-terminal pro-B-type natriuretic peptide (NT-proBNP) yielded excellent results. This novel ePAD, fabricated using inkjet printing technology on paper, simplifies the process, reduces environmental impact, and lowers fabrication costs. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
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