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Pharmaceutical wastewater is a typical type of wastewater with high concentrations of organic pollutants, but research on this subject is limited. The aeration tanks of three different pharmaceutical wastewater treatment systems were seeded with the same inocula and stably operated for 40 days. Then, aerobic sludge samples from the three aeration tanks were collected to provide insight into the bacterial community composition of the activated sludges. Additionally, we investigated the effects of wastewater characteristics and the type and operation of the technological system on the microbial communities. High-throughput sequencing analysis demonstrated that the communities enriched in the three reactors had differing. The dominant phyla detected were Proteobacteria, Chloroflexi, Bacteroidetes and candidate division TM7, while the dominant clones were uncultured Candidatus Saccharibacteria bacterium, uncultured Saprospiraceae bacterium, PHOS-HE51(AF314433.1), uncultured Anaerolineaceae bacterium and Blastocatella, suggesting their importance in pharmaceutical wastewater treatment plants. According to the wastewater parameters and canonical correspondence analyses, we can conclude that uncultured Candidatus Saccharibacteria bacterium, uncultured Anaerolineaceae bacterium and Blastocatella contribute to ammonium nitrogen ( ) removal; uncultured Saprospiraceae bacterium plays an important role in treating nitrogen; and chemical oxygen demand and PHOS-HE51 contribute to total phosphorus removal.

Gibberellin wastewater cannot be directly discharged without treatment due to its high concentrations of sulfate and organic compounds and strong acidity. Therefore, multi-stage anaerobic bioreactor + micro-aerobic+ anoxic/aeration (A/O) + biological contact oxidation combined processes are used to treat gibberellin wastewater. However, knowledge of the treatment effects of the A/O process and bacterial community structure in the aeration tank reactors of such systems is sparse. Therefore, this study was conducted to investigate the treatment effects and operation of the A/O process on gibberellin wastewater, as well as changes in the bacterial community structure of activated sludge in the aeration tank during treatment. Moreover, removal was examined based on evaluation of effluent after A/O treatment. Although influent chemical oxygen demand (COD), NH3-N and total phosphorus (TP) fluctuated, effluent COD, NH3-N and TP remained stable. Moreover, average COD, NH3-N and TP removal efficiency were 68.41%, 93.67% and 45.82%, respectively, during the A/O process. At the phylum level, Proteobacteria was the dominant phylum in all samples, followed by Chloroflexi, Bacteroidetes and Actinobacteria. Proteobacteria played an important role in the removal of organic matter. Chloroflexi was found to be responsible for the degradation of carbohydrates and Bacteroidetes also had been found to be responsible for the degradation of complex organic matters. Actinobacteria are able to degrade a variety of environmental chemicals. Additionally, Anaerolineaceae_uncultured was the major genus in samples collected on May 25, 2015, while Novosphingobium and Nitrospira were dominant in most samples. Nitrosomonas are regarded as the dominant ammonia-oxidizing bacteria, while Nitrospira are the main nitrite-oxidizing bacteria. Bacterial community structure varied considerably with time, and a partial Mantel test showed a highly significant positive correlation between bacterial community structure and DO. The bacterial community structure was also positively correlated with temperature and SO42-.

To treat gibberellin (GA) wastewater, a full-scale, multi-stage combined contact process was developed. This whole process employs three anaerobic reactors followed by micro-aerobic, anoxic/aeration and biological oxidation treatment. Pollutant removal results showed that the combined process could remove more than 98% of the chemical oxygen demand (COD), NH3N, and SO42- pollutants because of the different microbial communities in each reactor. 16S rRNA gene sequencing was used to examine the microbial communities in the internal circulation (IC) and in the two up flow anaerobic sludge blanket (UASB) reactors, as well as to investigate the effect of sampling elevation on the microbial community. The results showed that Firmicutes and Euryarchaeota were the most dominant phyla at the bacterial and archaeal levels, respectively. High levels of Synergistaceae_uncultured were detected in IC and UASB1. Chloroflexi_uncultured was the dominant genus of bacterial communities within UASB2, and Methanosaeta was the dominant genus of archaeal communities. Principal coordinates analysis (PCoA) revealed variations among the microbial communities in 9 samples, and Venn analysis showed different operational taxonomic units (OTUs) among samples collected at various elevations within the three anaerobic reactors. However, partial Mantel tests indicated no significant correlation between the microbial community structure and elevation in the three anaerobic reactors.