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5 results on '"Erkent, Alp"'

2. Identification of SERPINE1 as a regulator of glioblastoma cell dispersal with transcriptome profiling

Author

Department of Industrial Engineering, Şeker, Fidan; Cingöz, Ahmet; Sur Erdem, İlknur; Ergüder, Nazlı; Erkent, Alp; Önder, Tuğba Bağcı (ORCID 0000-0003-3646-2613 & YÖK ID 184359); Gönen, Mehmet (ORCID 0000-0002-2483-075X & YÖK ID 237468), Uyulur, Fırat; Selvan, Myvizhi Esa; Gümüş, Zeynep Hülya; Bayraktar, Halil; Wakimoto, Hiroaki, Department of Industrial Engineering, Şeker, Fidan; Cingöz, Ahmet; Sur Erdem, İlknur; Ergüder, Nazlı; Erkent, Alp; Önder, Tuğba Bağcı (ORCID 0000-0003-3646-2613 & YÖK ID 184359); Gönen, Mehmet (ORCID 0000-0002-2483-075X & YÖK ID 237468), and Uyulur, Fırat; Selvan, Myvizhi Esa; Gümüş, Zeynep Hülya; Bayraktar, Halil; Wakimoto, Hiroaki

Abstract

High mortality rates of glioblastoma (GBM) patients are partly attributed to the invasive behavior of tumor cells that exhibit extensive infiltration into adjacent brain tissue, leading to rapid, inevitable, and therapy-resistant recurrence. In this study, we analyzed transcriptome of motile (dispersive) and non-motile (core) GBM cells using an in vitro spheroid dispersal model and identified SERPINE1 as a modulator of GBM cell dispersal. Genetic or pharmacological inhibition of SERPINE1 reduced spheroid dispersal and cell adhesion by regulating cell-substrate adhesion. We examined TGFβ as a potential upstream regulator of SERPINE1 expression. We also assessed the significance of SERPINE1 in GBM growth and invasion using TCGA glioma datasets and a patient-derived orthotopic GBM model. SERPINE1 expression was associated with poor prognosis and mesenchymal GBM in patients. SERPINE1 knock-down in primary GBM cells suppressed tumor growth and invasiveness in the brain. Together, our results indicate that SERPINE1 is a key player in GBM dispersal and provide insights for future anti-invasive therapy design.

Published
2019

3. Identification of SERPINE1 as a Regulator of Glioblastoma Cell Dispersal with Transcriptome Profiling

Author

Seker, Fidan, primary, Cingoz, Ahmet, additional, Sur-Erdem, İlknur, additional, Erguder, Nazli, additional, Erkent, Alp, additional, Uyulur, Fırat, additional, Esai Selvan, Myvizhi, additional, Gümüş, Zeynep Hülya, additional, Gönen, Mehmet, additional, Bayraktar, Halil, additional, Wakimoto, Hiroaki, additional, and Bagci-Onder, Tugba, additional

Published
2019
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5. Identification of SERPINE1 as a regulator of glioblastoma cell dispersal with transcriptome profiling

Author

Ahmet Cingoz, Alp Erkent, Fırat Uyulur, Fidan Seker, Zeynep H. Gümüş, Myvizhi Esai Selvan, Ilknur Sur-Erdem, Halil Bayraktar, Mehmet Gönen, Tugba Bagci-Onder, Hiroaki Wakimoto, Nazli Erguder, Şeker, Fidan, Cingöz, Ahmet, Sur Erdem, İlknur, Ergüder, Nazlı, Erkent, Alp, Önder, Tuğba Bağcı (ORCID 0000-0003-3646-2613 & YÖK ID 184359), Gönen, Mehmet (ORCID 0000-0002-2483-075X & YÖK ID 237468), Uyulur, Fırat, Selvan, Myvizhi Esa, Gümüş, Zeynep Hülya, Bayraktar, Halil, Wakimoto, Hiroaki, Graduate School of Sciences and Engineering, College of Engineering, and Department of Industrial Engineering

Subjects
0301 basic medicine, Cancer Research, Cell, Regulator, Biology, urologic and male genital diseases, lcsh:RC254-282, Transcriptome, 03 medical and health sciences, transcriptome analysis, 0302 clinical medicine, Glioma, medicine, dispersal, Cell adhesion, Dispersal, GBM, Transcriptome analysis, urogenital system, Mesenchymal stem cell, Spheroid, gbm, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, medicine.disease, nervous system diseases, 030104 developmental biology, medicine.anatomical_structure, Oncology, 030220 oncology & carcinogenesis, Cancer research, Biological dispersal
Abstract

High mortality rates of glioblastoma (GBM) patients are partly attributed to the invasive behavior of tumor cells that exhibit extensive infiltration into adjacent brain tissue, leading to rapid, inevitable, and therapy-resistant recurrence. In this study, we analyzed transcriptome of motile (dispersive) and non-motile (core) GBM cells using an in vitro spheroid dispersal model and identified SERPINE1 as a modulator of GBM cell dispersal. Genetic or pharmacological inhibition of SERPINE1 reduced spheroid dispersal and cell adhesion by regulating cell-substrate adhesion. We examined TGFβ as a potential upstream regulator of SERPINE1 expression. We also assessed the significance of SERPINE1 in GBM growth and invasion using TCGA glioma datasets and a patient-derived orthotopic GBM model. SERPINE1 expression was associated with poor prognosis and mesenchymal GBM in patients. SERPINE1 knock-down in primary GBM cells suppressed tumor growth and invasiveness in the brain. Together, our results indicate that SERPINE1 is a key player in GBM dispersal and provide insights for future anti-invasive therapy design., Scientific and Technological Research Council of Turkey (TÜBİTAK); Koç University; Turkish Academy of Sciences (TÜBA), TÜBA-Gedip The Young Scientist Award Program) and the Science Academy of Turkey (BAGEP; The Young Scientist Award Program); LUNGevity foundation

Published
2019

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