Search

Objective: Hepatocellular carcinoma is the most common type of liver cancer. Doxorubicin (Dox) is an anthracycline antibiotic and is commonly used for cancer treatment. However, Dox has severe side effects that limit efficiency, and it is used with drug delivery systems. Asialoglycoprotein receptors are membrane glycoprotein receptors and are expressed in hepatocellular carcinoma. This study, it is aimed to develop a magnetic field and asialoglycoprotein receptor-targeted, doxorubicin-loaded nanoparticle system to be used in the treatment of hepatocellular carcinoma. Research methods: Magnetic nanoparticles were synthesized by the co-precipitation method. The surface was functionalized with 2-aminoethylphosphonic acid to provide the binding of arabinogalactan with magnetic nanoparticles. Then, MNP's surface was coated with arabinogalactan. After that, doxorubicin was loaded on arabinogalactan-coated magnetic nanoparticles by adsorption. The functional groups, hydrodynamic size, zeta potential, and drug encapsulation efficiency were evaluated. Results and Conclusion: The SEM size of the Dox-loaded arabinogalactan-coated magnetic nanoparticle(DANP) was found to be 30-35 nm. FTIR analysis has confirmed the presence of doxorubicin on arabinogalactan-coated ANPs. Dox loading efficiency was determined as 78.54 %. Based on the results obtained, the study is thought to have potential for in vitro, in vivo, and ex vivo studies. [ABSTRACT FROM AUTHOR]

Objective: Hepatocellular carcinoma (HCC) is the fourth most common cause of cancer-related death worldwide. The World Health Organization stated that by 2030, the number of deaths from hepatocellular carcinoma would be more than 1 million. It is known that there is a high amount of asialoglycoprotein receptors (ASPGR) on the hepatocyte surface, and they are intensely expressed in HCC. These properties make ASGPR an attractive target for receptor-directed drug delivery to hepatocarcinoma cells. New drug delivery systems are being developed to prevent the side effects and limitations of commonly used treatment methods. As an antineoplastic agent, doxorubicin is used for the treatment of many cancers. Research methods: This study aimed to perform in vitro cytotoxicity and biocompatibility tests of the synthesized asialoglycoprotein receptor-targeted and doxorubicin carrying magnetic nanoparticles to treat HCC. For this purpose, after the synthesis of magnetic nanoparticles loaded with doxorubicin, serum protein binding and hemolysis were assayed. The cytotoxicity studies were carried out on hepatocellular carcinoma cell, HEPG2, and human epithelial liver cell, THLE-2. Results and Conclusion: The cytotoxicity studies were carried out on hepatocellular carcinoma cell, HEPG2, and human epithelial liver cell, THLE-2. According to the results, it was found that the prepared formulation was biocompatible, had no hemolytic effect, and created cytotoxicity in cancer cells. As a result, it is thought that the doxorubicin-loaded asialoglycoprotein targeted magnetic nanoparticle system has a high potential for use in the treatment of hepatocellular carcinoma. [ABSTRACT FROM AUTHOR]