1. MS-based targeted metabolomics of eicosanoids and other oxylipins: Analytical and inter-individual variabilities
- Author
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Nils Helge Schebb, John W. Newman, Annika I. Ostermann, Cécile Gladine, Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Faculty of Mathematics and Natural Sciences, University of Wuppertal, United States Department of Agriculture, Agricultural Researh Service, Western Human Nutrition Research Center, Department of Nutrition, Harvard School of Public Health, Unité de Nutrition Humaine - Clermont Auvergne (UNH), and Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA)
- Subjects
thromboxanes ,SPM ,HETE ,PG ,Biochemistry ,TriHOME ,ethylenediaminetetraacetic acid ,GPCR ,0302 clinical medicine ,TRPs ,Specialized pro-resolving mediators ,Tandem Mass Spectrometry ,transient receptor potential channels ,CYP ,hydroxyeicosapentaenoic acid ,Profiling (information science) ,CAD ,Biomarker discovery ,Chromatography, High Pressure Liquid ,EDTA ,docosahexaenoic acid ,3. Good health ,coronary artery diseases ,electrospray ionization-mass spectrometry ,Fatty Acids, Unsaturated ,nuclear factor kappa B ,PD ,CRP ,BHT ,PPARs ,trihydroxyoctadecenoic acid ,lipoxin ,gas liquid chromatography mass spectrometry ,FLAP ,1 Abbreviations : (U)HPLC-MS ,hydroxyeicosatetraenoic acid ,03 medical and health sciences ,[CHIM.ANAL]Chemical Sciences/Analytical chemistry ,Physiology (medical) ,Humans ,Metabolomics ,G protein-coupled receptor ,maresin ,LLOQ ,5-lipoxygenase-activating protein ,hydroxydocosahexaenoic acid ,EPA ,AA ,lipoxygenase ,cardiovascular diseases ,030104 developmental biology ,Vasoconstriction ,EpETrE ,time of flight ,3-Hydroxy-3-Methyl-Glutaryl-CoA reductase ,C- reactive protein ,030217 neurology & neurosurgery ,NFκB ,Biological variability ,0301 basic medicine ,eicosapentaenoic acid ,QqQ-MS ,HEPE ,ketooctadecadienoic acid ,epoxyoctadecenoic acid ,Nuclear factor kappa b ,LX ,MaR ,GLC-MS ,LT ,leukotrienes ,Triple quadrupole mass spectrometry ,epoxyeicosatrienoic acid ,LOD ,HMG- CoA ,Observer Variation ,HODE ,limit of detection ,TOF ,soluble epoxyhydroalse ,LOX ,lower limit of quantification ,Neurodegenerative Diseases ,CVD ,Vasodilation ,DHA ,peroxisome proliferator-activated receptors ,prostaglandin ,polyunsaturated fatty acids ,cytochrome P450 ,butylated hydroxytoluene ,sEH ,Computational biology ,Biology ,HDHA ,arachidonic acid ,Oxylipins ,KODE ,hydroxyoctadecadienoic acid ,Inflammation ,triple quadrupole mass spectrometry ,protectin ,COX ,solid phase extraction ,Reproducibility of Results ,ESI-MS ,Tx ,(ultra) high performance liquid chromatography-mass spectrometry ,Oxylipin ,cycloxygenase ,specialized pro-resolving mediators ,PUFAs ,Eicosanoids ,SPE ,EpOME ,[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition ,Targeted metabolomics - Abstract
Epub ahead of print; Oxylipins, including the well-known eicosanoids, are potent lipid mediators involved in numerous physiological and pathological processes. Therefore, their quantitative profiling has gained a lot of attention during the last years notably in the active field of health biomarker discovery. Oxylipins include hundreds of structurally and stereochemically distinct lipid species which today are most commonly analyzed by (ultra) high performance liquid chromatography-mass spectrometry based ((U)HPLC-MS) methods. To maximize the utility of oxylipin profiling in clinical research, it is crucial to understand and assess the factors contributing to the analytical and biological variability of oxylipin profiles in humans. In this review, these factors and their impacts are summarized and discussed, providing a framework for recommendations expected to enhance the interlaboratory comparability and biological interpretation of oxylipin profiling in clinical research.
- Published
- 2019