99 results on '"Enzhong, Li"'
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2. Molecular characterization of the tet (M)-carrying transposon Tn7124 and plasmids in Escherichia coli isolates recovered from swine
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Yingying Liu, Zhu Qiao, Yan Ma, Mingcheng Wang, Gongzheng Hu, and Enzhong Li
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IncR-type plasmid ,tet (M) ,insertion sequence 26 ,transposon 7124 ,Escherichia coli ,Veterinary medicine ,SF600-1100 - Abstract
Here, we report the genetic features and evolutionary mechanisms of two tet (M)-bearing plasmids (pTA2 and pTA7) recovered from swine Escherichia coli isolates. The genetic profiles of pTA2 and pTA7 and corresponding transconjugants were accessed by S1 nuclease pulsed-field gel electrophoresis and Southern hybridization, followed by whole genome sequencing and bioinformatics analysis. The biological influences of pTA2 and pTA7 were determined by stability and direct competition assays. Both pTA7 and pTA2 had the IncR backbone sequences but differed in the multidrug resistance region (MDR). The MDR of pTA2 consisted of sul3, tet (M), qnrS1, bleO, oqxAB, floR, aadA1, cmlA1, aadA2, and tet (A)-tetR (A) in addition to 22 insertion sequences. Notably, pTA2 carried the novel complex Tn7124 (IS26-ctp-lp-tet (M)-hp-IS406tnp-IntI4-IS26) harboring tet (M). The fragment carrying tet (M) (IS26-ctp-lp-tet (M)-IS406 tnp-ctp-aadA1-cmlA1-aadA2-dfrA12-IntI1), named Tn6942-like, and the two resistance modules ISVsa3-VirD2-floR-lysR and tet (A)-tetR (A) were located in the MDR of pTA7. Both pTA2 and pTA7 were highly stable in E. coli DH5α cells with no fitness cost to the host or disadvantage in growth competition. These results indicate that transposons carrying tet (M) continuously integrate via mediation with an insertion sequence, which accelerates the transmission of tet (M) in E. coli isolates through integration of other drug-resistant genes, thereby posing a potential serious threat to the efficacy of clinical treatment.
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- 2024
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3. Pyridaben inhibits cell cycle progression and delays early embryonic development in zebrafish (Danio rerio)
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Weidong Qiang, Wenwen Wang, Tianzhu Shen, Shuhui Wu, Shengnan Yu, Xiaomei Zhang, Yang Yang, Xiaokun Li, Enzhong Li, and Fanghua Gong
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Pyridaben ,Zebrafish ,Embryonic developmental delay ,Cell cycle order ,Environmental pollution ,TD172-193.5 ,Environmental sciences ,GE1-350 - Abstract
Pyridaben is a broad-spectrum, contact-killing acaricide that can be used to control a variety of harmful food and plant mites. Pyridaben displays cardiotoxicity and liver toxicity toward fish, but the effects on fish embryonic development have not been characterized. We exposed early zebrafish embryos to 20, 30, and 40 μg/L concentrations of pyridaben. The exposure caused developmental abnormalities, including delayed embryonic shield formation, yolk sac resorption, decreases in body length, reduced pigmentation, and delays in hatching. Pyridaben caused a significant increase in the transcription level of the endoderm marker foxa2, but the transcription levels of the ectoderm development marker foxb1a and the mesoderm development marker snaila were not significantly altered. The transcription levels of the genes SOX17 in early embryos were significantly reduced. After exposure to pyridaben, catalase (CAT) activity and glutathione (GSH) content were increased, and cyclin D1, that is involved in early embryonic development, was abnormally expressed. This study shows that pyridaben causes anomalous development in zebrafish embryos by interfering with the cell cycle order of early embryonic development and inducing excessive oxidative stress. Colivelin, an agonist of the STAT3 signaling pathway, acted as a salvage drug to restore the cell cycle order during embryonic development following exposure to pyridaben. Thus, the toxic effects may be caused by pyridaben’s regulation of the STAT3 signaling pathway.
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- 2024
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4. Compound probiotics regulate the NRF2 antioxidant pathway to inhibit aflatoxin B1-induced autophagy in mouse Sertoli TM4 cells
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Hongwei Guo, Taiyang Liu, Jie Li, Enzhong Li, Xiuhua Wen, Fujia Chen, Siqaing Li, Yun Li, Qingqiang Yin, and Qun Zhu
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Compound probiotics ,Aflatoxin B1 ,Sertoli TM4 cells ,Antioxidant pathway ,Autophagy ,Environmental pollution ,TD172-193.5 ,Environmental sciences ,GE1-350 - Abstract
This study investigated the effects of compound probiotics (CP) on AFB1-induced cytotoxicity in Sertoli TM4 cells. The L9 (3 × 3) orthogonal test was conducted to determine the optimal CP required for high AFB1 degradation in the artificial gastrointestinal fluid in vitro. The maximal AFB1 degradation rate was 40.55 % (P < 0.05) when the final viable count was 1.0 × 105 CFU/mL for Bacillus subtilis, Lactobacillus casein, and Saccharomyces cerevisiae. The effects of CP and the CP supernatant (CPS) on TM4 cell viability were evaluated to achieve the optimal protective conditions. When CPS4 (corresponding to CP viable counts of 1.0 × 104 CFU/mL) was added to the TM4 cells for 24 h, the cell viability reached 108.86 % (P < 0.05). AFB1 reduced TM4 cell viability in a concentration- and time-dependent manner at an AFB1 concentration ranging from 0 to 1.5 μM after 48-h AFB1 exposure. The optimal AFB1 concentration/times for low- and high damage models were 0.5 and 1.25 μM both for 24 h, which decreased viability to 76.04 % and 65.35 %, respectively. however, CPS4 added to low- and high-damage models increased the cell viability to 97.43 % and 75.12 %, respectively (P < 0.05). Transcriptome sequencing was performed based on the following designed groups: the control, 0.5 μM AFB1, 1.25 μM AFB1, CPS4, and CPS4+0.5 μM AFB1. The Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analysis was further performed to identify significantly enriched signaling pathways, which were subsequently verified. It was shown that AFB1 induced apoptosis by blocking the PI3K-AKT-mTOR pathway and upregulating autophagy proteins such as LC3B, Beclin1, and ATG5 while inhibiting autophagic flux. CPS4 promoted AFB1 degradation, activated the p62-NRF2 antioxidant, and inhibited ROS/TRPML1 pathways, thereby reducing ROS production and inflammation and ultimately alleviating AFB1-induced autophagy and apoptosis. These findings supports the potential of probiotics to protect the male reproductive system from toxin damage.
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- 2024
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5. Identifying potential therapeutic targets in lung adenocarcinoma: a multi-omics approach integrating bulk and single-cell RNA sequencing with Mendelian randomization
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Youpeng Chen, Enzhong Li, Zhenglin Chang, Tingting Zhang, Zhenfeng Song, Haojie Wu, Zhangkai J. Cheng, and Baoqing Sun
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lung adenocarcinoma ,bulk RNA sequencing ,Mendelian randomization ,single-cell RNA sequencing ,potential therapeutic targets ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Our research aimed to identify new therapeutic targets for Lung adenocarcinoma (LUAD), a major subtype of non-small cell lung cancer known for its low 5-year survival rate of 22%. By employing a comprehensive methodological approach, we analyzed bulk RNA sequencing data from 513 LUAD and 59 non-tumorous tissues, identifying 2,688 differentially expressed genes. Using Mendelian randomization (MR), we identified 74 genes with strong evidence for a causal effect on risk of LUAD. Survival analysis on these genes revealed significant differences in survival rates for 13 of them. Our pathway enrichment analysis highlighted their roles in immune response and cell communication, deepening our understanding. We also utilized single-cell RNA sequencing (scRNA-seq) to uncover cell type-specific gene expression patterns within LUAD, emphasizing the tumor microenvironment’s heterogeneity. Pseudotime analysis further assisted in assessing the heterogeneity of tumor cell populations. Additionally, protein-protein interaction (PPI) network analysis was conducted to evaluate the potential druggability of these identified genes. The culmination of our efforts led to the identification of five genes (tier 1) with the most compelling evidence, including SECISBP2L, PRCD, SMAD9, C2orf91, and HSD17B13, and eight genes (tier 2) with convincing evidence for their potential as therapeutic targets.
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- 2024
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6. Novel Insights into Ethanol-Soluble Oyster Peptide–Zinc-Chelating Agents: Structural Characterization, Chelation Mechanism, and Potential Protection on MEHP-Induced Leydig Cells
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Zhen Lu, Qianqian Huang, Xiaoming Qin, Fujia Chen, Enzhong Li, and Haisheng Lin
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zinc-chelating peptides ,MEHP ,TM3 ,in silico screening ,apoptosis ,Biology (General) ,QH301-705.5 - Abstract
Numerous studies have reported that mono-(2-ethylhexyl) phthalate (MEHP) (bioactive metabolite of Di(2-ethylhexyl) phthalate) has inhibitory effects on Leydig cells. This study aims to prepare an oyster peptide–zinc complex (PEP-Zn) to alleviate MEHP-induced damage in Leydig cells. Zinc-binding peptides were obtained through the following processes: zinc-immobilized affinity chromatography (IMAC-Zn2+), liquid chromatography–mass spectrometry technology (LC-MS/MS) analysis, molecular docking, molecular dynamic simulation, and structural characterization. Then, the Zn-binding peptide (PEP) named Glu—His—Ala—Pro—Asn—His—Asp—Asn—Pro—Gly—Asp—Leu (EHAPNHDNPGDL) was identified. EHAPNHDNPGDL showed the highest zinc-chelating ability of 49.74 ± 1.44%, which was higher than that of the ethanol-soluble oyster peptides (27.50 ± 0.41%). In the EHAPNHDNPGDL-Zn complex, Asn-5, Asp-7, Asn-8, His-2, and Asp-11 played an important role in binding to the zinc ion. Additionally, EHAPNHDNPGDL-Zn was found to increase the cell viability, significantly increase the relative activity of antioxidant enzymes and testosterone content, and decrease malondialdehyde (MDA) content in MEHP-induced TM3 cells. The results also indicated that EHAPNHDNPGDL-Zn could alleviate MEHP-induced apoptosis by reducing the protein level of p53, p21, and Bax, and increasing the protein level of Bcl-2. These results indicate that the zinc-chelating peptides derived from oyster peptides could be used as a potential dietary zinc supplement.
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- 2024
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7. Analysis and enhancement of the energy utilization efficiency of corn stover using strain Lsc-8 in a bioelectrochemical system
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Lianbin Cao, Hongmei Sun, Yamei Ma, Mingguo Lu, Mengrui Zhao, Enzhong Li, and Ying Liu
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Microbial fuel cells ,Cellulose ,Raw corn stover ,Geobacter sulfurreducens PCA ,Cellulomonas sp. ,Microbiology ,QR1-502 - Abstract
Abstract The strain Lsc-8 can produce a current density of 33.08 µA cm−2 using carboxymethylcellulose (CMC) as a carbon source in a three-electrode configuration. A co-culture system of strain Lsc-8 and Geobacter sulfurreducens PCA was used to efficiently convert cellulose into electricity to improve the electricity generation capability of microbial fuel cells (MFCs). The maximum current density achieved by the co-culture with CMC was 559 μA cm−2, which was much higher than that of strain Lsc-8 using CMC as the carbon source. The maximum power density reached 492.05 ± 52.63 mW cm−2, which is much higher than that previously reported. Interaction mechanism studies showed that strain Lsc-8 had the ability to secrete riboflavin and convert cellulose into acetic acid, which might be the reason for the high electrical production performance of the co-culture system. In addition, to the best of our knowledge, a co-culture or single bacteria system using agricultural straw as the carbon source to generate electricity has not been reported. In this study, the maximum current density of the three-electrode system inoculated with strain Lsc-8 was 14.56 μA cm−2 with raw corn stover as the sole carbon source. Raw corn stover as a carbon source was also investigated for use in a co-culture system. The maximum current density achieved by the co-culture was 592 μA cm−2. The co-culture system showed a similar electricity generation capability when using raw corn stover and when using CMC. This research shows for the first time that a co-culture or single bacteria system can realize both waste biomass treatment and waste power generation.
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- 2023
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8. Impact of different dietary fibre levels on the roughage resistance of the Dahe black pig
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Ye Wang, Chaoying Liu, Jinjin Zhu, Enzhong Li, Mingcheng Wang, Zhichao Shen, Xinfeng Yang, Guojin Lv, and Rongfu Guo
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production performance ,nutrient digestibility ,prkag3 ,fsp27 ,chinese milk vetch ,gene expression ,Animal culture ,SF1-1100 - Abstract
To investigate the effects and molecular mechanism of different levels of dietary fibre (Chinese milk vetch) on Dahe black and Duroc × (Landrace × Yorkshire) (DLY) pigs, 54 of each type of pig were selected and fed diets that contained 3.5%, 5.5% and 7.5% levels of fibre in the growing (30-60 kg) and fattening stages (60-120 kg). The production performance, serum physicochemical indices, nutrient digestibility, the amylase activity in the small intestine, contents of the muscle crude protein and ether extract, and the levels of transcription of the PRKAG3 and Fsp27 genes were determined. The production performance of the Dahe black pigs with a 5.5% level of dietary fibre was significantly higher than those with dietary fibre levels of 3.5% and 7.5%. A diet high in fibre also significantly affected the production performance of the DLY pigs. The apparent digestibility of the nutrients decreased with an increase in the dietary fibre level, and the Dahe black pigs appeared to more effectively digest the dietary fibre than the DLY pigs. The serum physicochemical indices, amylase activity, and the expression levels of the PRKAG3 and Fsp27 genes from the Dahe black pigs were significantly higher than those of the DLY pigs, and the intramuscular fat content of the Dahe black pigs fed a high fibre diet was significantly higher than that of the DLY pigs. The dietary fibre levels of 5.5% and 7.5% did not affect the production performance of the Dahe black pigs during the 30-120 kg period, but they significantly reduced the production performance of the DLY pigs. The Dahe black pigs were evidently tolerant to high amounts of fibre when fed a high fibre diet.
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- 2022
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9. Oyster Peptide-Zinc Complex Ameliorates Di-(2-ethylhexyl) Phthalate-Induced Testis Injury in Male Mice and Improving Gut Microbiota
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Zhen Lu, Qianqian Huang, Fujia Chen, Enzhong Li, Haisheng Lin, and Xiaoming Qin
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oyster peptide-zinc complex ,DEHP ,testis injury ,zinc ,intestinal microbiota ,Chemical technology ,TP1-1185 - Abstract
Di-(2-ethylhexyl) phthalate (DEHP) is a widely used plasticizer, which can cause damage to male reproductive organs, especially the atrophy of the testis. Meanwhile, DEHP can also lead to a decrease in testicular zinc content, but the role of zinc remains unclear. This study aims to prepare oyster peptide-zinc complex (OPZC) to alleviate DEHP-induced reproductive damage in mice. OPZC was successfully obtained through electron microscopy, X-ray diffraction, and thermogravimetric analysis, with stable structure and high water-solubility. Low dose oyster peptide-zinc complex (OPZCL) significantly reduced the reproductive damage caused by DEHP in mice. Further research had shown that OPZCL restored the content of serum hormones and the activity of oxidative stress kinases to normal, while also normalizing testicular zinc and selenium levels. In addition, it also recovered the disorder of gut microbiota, reduced the proportion of Bacteroides, increased the abundance of Ligilactobacillus, and restored the proportion of Acidobacteriota, Chloroflexi, and Proteobacteria. Therefore, OPZCL can relieve the reproductive damage caused by DEHP in mice by restoring testicular zinc homeostasis and the composition of intestinal microbiota, indicating that OPZCL has a potential protective effect on male reproductive health.
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- 2023
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10. Physiological and transcriptional studies reveal Cr(VI) reduction mechanisms in the exoelectrogen Cellulomonas fimi Clb-11
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Lianbin Cao, Mingguo Lu, Mengrui Zhao, Yifan Zhang, Yiping Nong, Mengxue Hu, Ya Wang, Tongbiao Li, Fujia Chen, Mingcheng Wang, Junhe Liu, Enzhong Li, and Hongmei Sun
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microbial fuel cells ,Cr(VI) reduction ,Cellulomonas ,cell swelling ,transcriptome ,Microbiology ,QR1-502 - Abstract
A facultative exoelectrogen, Cellulomonas fimi strain Clb-11, was isolated from polluted river water. This strain could generate electricity in microbial fuel cells (MFCs) with carboxymethyl cellulose (CMC) as the carbon source, and the maximum output power density was 12.17 ± 2.74 mW·m−2. In addition, Clb-11 could secrete extracellular chromate reductase or extracellular electron mediator to reduce Cr(VI) to Cr(III). When the Cr(VI) concentration was less than 0.5 mM in Luria-Bertani (LB) medium, Cr(VI) could be completely reduced by Clb-11. However, the Clb-11 cells swelled significantly in the presence of Cr(VI). We employed transcriptome sequencing analysis to identify genes involved in different Cr(VI) stress responses in Clb-11. The results indicate that 99 genes were continuously upregulated while 78 genes were continuously downregulated as the Cr(VI) concentration increased in the growth medium. These genes were mostly associated with DNA replication and repair, biosynthesis of secondary metabolites, ABC transporters, amino sugar and nucleotide sugar metabolism, and carbon metabolism. The swelling of Clb-11 cells might have been related to the upregulation of the genes atoB, INO1, dhaM, dhal, dhak, and bccA, which encode acetyl-CoA C-acetyltransferase, myo-inositol-1-phosphate synthase, phosphoenolpyruvate-glycerone phosphotransferase, and acetyl-CoA/propionyl-CoA carboxylase, respectively. Interestingly, the genes cydA and cydB related to electron transport were continuously downregulated as the Cr(VI) concentration increased. Our results provide clues to the molecular mechanism of Cr(VI) reduction by microorganisms in MFCs systems.
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- 2023
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11. Isolation and Identification of Bacteria Leading to Microbial Spoilage of Sweet Potato and Screening for Antistaling Agents Running Head
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Daoqi Liu, Huili Liu, Yuanyuan Dang, Mingcheng Wang, Huili Xia, Shuyu Shi, Enzhong Li, Daoqi Liu, Huili Liu, Yuanyuan Dang, Mingcheng Wang, Huili Xia, Shuyu Shi, and Enzhong Li
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To develop natural preservatives tailored for sweet potatoes, the control effect of essential oils on the spoilage microorganisms of sweet potatoes is uncertain. Therefore, we first isolated and identified the dominant spoilage bacteria from spoiled sweet potatoes. Micrococcus aloeverae, Priestia aryabhattai, and Glutamicibacter arilaitensis were identified through screening, morphological observation, gram staining, and 16S rDNA assays. Subsequently, five plant essential oils (citral, Litseacubeba, parsley, eugenol, and chinaberry tree oils) that significantly inhibited the spoilage bacterial species were identified after screening 20 plant essential oils using the zone of inhibition test. Minimum Inhibitory Concentrations (MICs) of Litsea cubeba, eugenol, and parsley oils against the above three spoilage bacteria were 320-1280, 640-1280, and 320-640 μL/L, respectively. The three essential oils exhibiting the most favorable inhibitory effects were selected for combinatorial studies. Finally, the response surface methodology was used to optimize the combination concentration of Litsea cubeba, eugenol, and parsley oils. The optimum concentration was identified as follows: 320 μL/L for Litsea cubeba oil, 716 μL/L for parsley oil, and 1280 μL/L for eugenol oil. At this concentration, the growth of three spoilage bacteria was almost completely inhibited (OD values of 0.067, 0.202, and 0.013). The optimized compound essential oil exhibited potent inhibitory activity against the spoilage bacteria of sweet potatoes. The aforementioned studies may provide important guidance for the development of efficient and broad-spectrum sweet potato preservatives.
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- 2024
12. The Supportive Role of International Government Funds on the Progress of Sepsis Research During the Past Decade (2010–2019): A Narrative Review
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Yuxin Leng PhD, Chengjin Gao MD, Fang Li PhD, Enzhong Li PhD, and Fengzhu Zhang MSc
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Public aspects of medicine ,RA1-1270 - Abstract
This narrative review aimed to clarify the characteristics of international government support for sepsis research, trends in published literature on sepsis, and potential contributions of government-source grants to progress in sepsis research between fiscal years 2010 and 2019. The data in this study were collected from the National Institutes of Health (NIH, https://projectreporter.nih.gov/reporter.cfm/ ) of the United States of America (USA), National Natural Science Foundation of China (NSFC, https://isisn.nsfc.gov.cn/egrantweb/ ), and Japan Society for the Promotion of Science (JSPS, https://kaken.nii.ac.jp/ ). All sepsis-related projects approved by the NIH, NSFC, and JSPS were retrieved by searching the project titles, abstracts, and key words for “sepsis,” “septic shock,” or “sepsis inflammatory response syndrome” between 2010 and 2019. Representative sepsis-related studies published between Jan 2010 and Aug 2020 by the first/corresponding authors from these countries were obtained by searching the PubMed database using Medical Subject Heading terms for “sepsis” in representative journals, including Nature , Cell, Science, The Lancet, New England Journal of medicine (New Engl J Med), The Journal of American Medical Association (JAMA), Critical Care Medicine (CCM), Intensive Care Medicine (ICM), Chest, Annals of Emergency Medicine (Ann Emerg Med), and American Thoracic Society journals (ATS). The total/annual institutional budgets, major funding mechanisms and schemes, superior institutions and individual principal investigators, and published original research articles in the field of sepsis in the USA, China, and Japan during the past decade were investigated. The national supporting schemes of the NIH, NSFC, and JSPS were similar. Support from these institutions is quite important for the development of the field of “sepsis” which was acknowledged in 57–64% of original research articles published in CCM. For the future development of precision medicine in sepsis, more government funding support is necessary.
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- 2022
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13. Effects of resveratrol on reducing spermatogenic dysfunction caused by high-intensity exercise
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Yuping Guo, Anli Wang, Xinpeng Liu, and Enzhong Li
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Resveratrol ,High-intensity exercise ,Spermatogenesis ,iTRAQ proteomic analysis ,Gynecology and obstetrics ,RG1-991 ,Reproduction ,QH471-489 - Abstract
Abstract Background Long-term high-intensity exercise can lead to reproductive endocrine and spermatogenic dysfunction. This research is to investigate the effect of resveratrol on the reduction of reproductive dysfunction induced by high-intensity exercise, and to screen relevant factors and signal transduction pathways. Methods Rats were randomly divided into three groups, a control group, an intensive exercise group (IE group), and a resveratrol-treated group (RSV group). After 9 weeks of exercise, the sperm density and reproductive hormone concentrations were measured, along with antioxidation, inflammatory cytokine production, and histological analyses performed for each group. In addition, a proteomics analysis of the IE group and RSV group were conducted. Results We found that compared with the control group, the average sperm density (P
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- 2019
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14. Improving Thermostability of GH11 Xylanase XynASP by the Design of Loop Region
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Tongbiao Li, Siwen Yang, Xiaoxiao Wang, Hongxuan Cai, Ye Wang, Chao Li, and Enzhong Li
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GH11 xylanase ,thermostability ,loop ,site-directed mutagenesis ,protein engineering ,Crystallography ,QD901-999 - Abstract
Highly flexible loop regions can affect the structure-function relationship of enzymes. In this study, to reveal the effect of the loop on the thermal stability of GH11 xylanase XynASP from Aspergillus saccharolyticus JOP 1030-1, three mutants (T41V, A79Y, T81Q) located in the loop region were predicted by the FireProt web server and constructed by site-directed mutagenesis. Heat tolerance experiments were performed in the mutants and wild-type XynASP, as well as for previously reported mutant T41W, indicating that the thermostability of enzymes from weak to strong was wild-type XynASP, T41V, T81Q, A79Y and T41W. Novel hydrophobic contacts and hydrogen bonds in the single mutants were found and explained for enhanced thermostability. T41W, A79Y and T81Q were combined by site-directed mutagenesis. The combined double mutants T41W/A79Y and A79Y/T81Q displayed significantly higher thermostability than that of the wild-type, yet lower than that of the robust mutant T41W after 30 min of incubation at 35–60 °C. But the triple mutant T41W/A79Y/T81Q displayed a slight improvement in thermal stability compared to T41W after 30 min of incubation at 35–60 °C. Meanwhile, T41W/A79Y/T81Q exhibited substantially improved thermostability with a half-life of inactivation enhanced from 20.7 min to 127.0 min at 45 °C. Furthermore, the optimum temperature increased by 10 °C compared to the wild-type XynASP. The pH stability of T41W/A79Y/T81Q at pH 3.0-8.0 all obtained more than 88% residual activities, displaying a significant improvement compared to the wild-type XynASP (40–70% residual activities) at the same conditions. This study confirms that designing GH11xylanases in the loop region is an effective strategy for obtaining thermostabilizing xylanases.
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- 2022
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15. Rhamnose-Containing Compounds: Biosynthesis and Applications
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Siqiang Li, Fujia Chen, Yun Li, Lizhen Wang, Hongyan Li, Guofeng Gu, and Enzhong Li
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rhamnose ,deoxythymidinediphosphate-L-rhamnose ,guanosine diphosphate rhamnose ,uridine diphosphate-rhamnose ,rhamnosyltransferase ,rhamnose biosynthesis ,Organic chemistry ,QD241-441 - Abstract
Rhamnose-associated molecules are attracting attention because they are present in bacteria but not mammals, making them potentially useful as antibacterial agents. Additionally, they are also valuable for tumor immunotherapy. Thus, studies on the functions and biosynthetic pathways of rhamnose-containing compounds are in progress. In this paper, studies on the biosynthetic pathways of three rhamnose donors, i.e., deoxythymidinediphosphate-L-rhamnose (dTDP-Rha), uridine diphosphate-rhamnose (UDP-Rha), and guanosine diphosphate rhamnose (GDP-Rha), are firstly reviewed, together with the functions and crystal structures of those associated enzymes. Among them, dTDP-Rha is the most common rhamnose donor, and four enzymes, including glucose-1-phosphate thymidylyltransferase RmlA, dTDP-Glc-4,6-dehydratase RmlB, dTDP-4-keto-6-deoxy-Glc-3,5-epimerase RmlC, and dTDP-4-keto-Rha reductase RmlD, are involved in its biosynthesis. Secondly, several known rhamnosyltransferases from Geobacillus stearothermophilus, Saccharopolyspora spinosa, Mycobacterium tuberculosis, Pseudomonas aeruginosa, and Streptococcus pneumoniae are discussed. In these studies, however, the functions of rhamnosyltransferases were verified by employing gene knockout and radiolabeled substrates, which were almost impossible to obtain and characterize the products of enzymatic reactions. Finally, the application of rhamnose-containing compounds in disease treatments is briefly described.
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- 2022
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16. Research on the application of the blended <scp>BOPPPS</scp> based on an online and offline mixed teaching model in the course of fermentation engineering in applied universities
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Siqiang Li, Quanlan Liu, Shuang Guo, Yun Li, Fujia Chen, Chunhong Wang, Mingcheng Wang, Junhe Liu, Xianli Liu, Duanhao Wang, and Enzhong Li
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Molecular Biology ,Biochemistry - Published
- 2023
17. Screening of Dominant Cellulose-Degrading Microbe in Humus and Optimisation of its Enzyme Producing Conditions and Application Optimization of Enzyme Production Conditions and Evaluation of Efficient Straw Degradation by Penicillium oxalicum
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Gailing Wang, Chendi Li, Yanan Guo, Lei Guo, Mingcheng Wang, Tongbiao Li, Linglong Xu, and Enzhong Li
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Biochemistry ,Biotechnology - Published
- 2023
18. Effects of resveratrol on hydrogen peroxide-pyroptosis, apoptosis, and autophagy.
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Chaoying Liu, Ye Wang, Dahong Li, Tongbiao Li, Mingcheng Wang, Zhiwen Zhou, Qianyi Jin, and Enzhong Li
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APOPTOTIC bodies ,INTERSTITIAL cells ,CELL anatomy ,GERM cells ,HEMATOXYLIN & eosin staining - Abstract
Oxidative stress is one of the main causes of testicular spermatogenesis disorders. Resveratrol (RES) can inhibit oxidative stress levels, reduce germ cell damage, and promote spermatogenesis. To investigate the molecular mechanism by which RES promotes spermatogenesis, the effects of hydrogen peroxide (H
2 O2 ) on the pathological damage of mouse testis tissue were assessed. The expression of key genes associated with pyroptosis, apoptosis, and autophagy, and the effects of RES on this process were determined. Experimental mice were divided into three groups with intraperitoneally injection of H2 O2 (3 mmol/kg/d), RES (100 mg/kg/d) + H2 O2 (3 mmol/kg/d), and normal saline, respectively. The testicular tissues were collected, and the pathological changes were observed by hematoxylin and eosin staining. The ultrastructure was observed using transmission electron microscopy (TEM). The transcriptional levels of pyroptosis, apoptosis, and autophagy-related genes were measured and analyzed by fluorescence quantitative polymerase chain reaction. The results showed that, in the H2 O2 group, the basal membrane of spermatogenic tubules was damaged, atrophic, and thin. Interstitial cells were fragmented, and the gap of spermatogenic tubules increased. Compared with the H2 O2 group, the pathological damage of testicular tissue structure in the H2 O2 + RES group was significantly ameliorated. TEM results showed no evident damage to the cell structure in the control group, while apoptosis, autophagy, and pyroptosis were observed in the testicular tissue of H2 O2 and H2 O2 + RES groups, where cell damage was less, but apoptotic bodies, autophagosomes, and pyroptosomes were observed. The transcription levels of key pyroptosis genes increased in the H2 O2 group but decreased significantly after RES treatment. The results indicated that H2 O2 could induce pyroptosis, while RES inhibited pyroptosis and led to the release of inflammatory factors by regulating the expression of genes in signaling pathways. After H2 O2 injection, the transcript levels of key apoptosis genes in testicular tissue were lower than those in the control group, while RES promoted the occurrence of H2 O2 -induced apoptosis, to achieve the purpose of protecting itself from oxidative damage. H2 O2 could promote autophagy in mouse testicular tissue. RES enhanced the level of H2 O2 -induced autophagy, removed the damaged cells in the testicular tissue, and protected the development of the body. This study provided the experimental basis for the study of RES in oxidative damage, pyroptosis, apoptosis, and autophagy, and theoretical guidance and new ideas for drug development and treatment of male reproductive diseases in clinical practice. [ABSTRACT FROM AUTHOR]- Published
- 2024
19. Beverage containing ginger and snow pear inhibits smoke-induced pyroptosis in rat lungs and protects small intestine.
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Ye Wang, Chaoying Liu, Tongbiao Li, Dahong Li, Huili Xia, Mingcheng Wang, Fujia Chen, Siqiang Li, Yun Li, and Enzhong Li
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TOBACCO smoke ,CIGARETTE smoke ,SMALL intestine ,SMOKING ,PATHOLOGICAL physiology - Abstract
Tobacco smoke is the leading risk factor for lung cancer, accounting for over 80% of lung cancer deaths. Reducing the damage of cigarette smoke to the lungs through dietary therapy is currently a research hotspot. It has been reported that cigarette smoke extract induced inflammation and contributed to pyroptosis through the ROS/NLRP3/caspase-1 pathway in human bronchial epithelial cells. To investigate the effect of ginger and snow pear beverage on smoke-induced pyroptosis, ginger, snow pear, loquat, honey, and skimmed milk powder were used to prepare a lung moistening drink with excellent palatability. Male rats were divided into the drink group and control group and exposed to cigarette smoke for 60 days. Blood biochemical indices and organ coefficients were measured, and lung and small intestine tissues were excised for HE staining. The mRNA expression of pyroptosis in the lung tissue was evaluated by fluorescence quantitative polymerase chain reaction (qPCR). The results showed that globulin and urea nitrogen levels were significantly higher in control (C) group than that in beverage (B) group, while the opposite effect was observed for the albumin/globulin ratio and cholesterol level. The changes in the weight of the lung tissue were obvious and significantly higher in group B than that in group C. The histological structure of the lung tissue in group C was abnormal with evidence of significant pathological changes in the alveolar wall and capillary bronchial tube wall along with a few inflammatory cells. The pathological changes in group B showed significantly improved. The small intestinal villus length, intestinal crypt depth, goblet cell number, and V/C value were significantly higher in group B. In addition, the drink significantly reduced the mRNA expression of NLRP3, caspase1, GSDMD, IL-18, and IL-1β genes in the lung tissue. The results indicated that the tested drink exerted protective effects on the lungs and small intestine, which inhibited the release of inflammatory factors from the cellular pyroptosis signaling pathway, alleviated the occurrence of pyroptosis, protected the lung tissue, and reduced the cigarette smoke-induced damage and lesions. [ABSTRACT FROM AUTHOR]
- Published
- 2024
20. Extraction of Radix trichosanthis Polysaccharides for Potential Antihyperlipidemic Application
- Author
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Fujia Chen, Yilin Xu, Nana Ding, Hongyan Li, Tongbiao Li, Fengyun Liu, Mengxue Liang, Li Song, Junhe Liu, Enzhong Li, and Jiayang Liu
- Subjects
Article Subject ,General Immunology and Microbiology ,General Medicine ,General Biochemistry, Genetics and Molecular Biology - Abstract
This study focused on the optimization of ultrasound-assisted compound enzyme extraction for polysaccharides (RTPs) from Radix trichosanthis by orthogonal experiment and response surface methodology, and then its extraction kinetics model and antihyperlipidemic activities were studied. The optimum extraction process was as follows: cellulase—1.0%, papain—1.0%, pectase—0.5%, pH—5, extraction temperature—50°C, and liquid-to-solid ratio—30 mL/g; prediction value of RTPs was 7.54%; the experimental yield of RTPs was 7.22%, while 50 minutes was optimized in Weibull kinetics model. Then high-dose groups of RTP extract could reduce the TC, TG, and LDL-C levels and increase the level of HDL-C in high-fat mice, with the ability to lower the MDA content and enhance SOD level.
- Published
- 2022
21. Ferroptosis’s Role in Genitourinary System Cancer
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Chaoying Liu, Xinfeng Yang, Ye Wang, Keyu Wu, Siqiang Li, Gailing Wang, Yun Li, Chuanfeng Li, Mingcheng Wang, and Enzhong Li
- Subjects
Oncology - Published
- 2022
22. Antifungal Mechanism of Cinnamon Essential Oil against Chinese Yam-Derived Aspergillus niger
- Author
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Mingcheng Wang, Huiyuan Liu, Yuanyuan Dang, Dahong Li, Zhu Qiao, Gailing Wang, Guo Liu, Jin Xu, and Enzhong Li
- Subjects
Article Subject ,General Chemical Engineering ,General Chemistry ,Food Science - Abstract
Chinese yam with damaged outer skin can be easily oxidized and infected by spoilage fungi. To find preservatives in the storage of the Chinese yam, Aspergillus niger 103 was isolated, identified, and determined as the dominant spoilage fungus in Chinese yam according to Koch’s postulates. Then, the strain was used as a model to screen antifungal agents and study antifungal mechanisms in this study. We found that cinnamon essential oil was the best antifungal agent, and the minimum concentration against Aspergillus niger 103 was 25 μg/mL. The storage life of Chinese yam could significantly extend by 27.66 days by spraying with cinnamon essential oil (25 μg/mL). To further explore the antifungal mechanism of cinnamon essential oil against Aspergillus niger 103, alkaline phosphatase activity and electrolyte content in the fungal solution were measured. The alkaline phosphatase activity and electrolyte content of the fungal solution with cinnamon essential oil were significantly increased than those without cinnamon essential oil, which showed that the cinnamon essential oil could destroy the integrity of the cell wall and cell membrane of Aspergillus niger 103, and disrupted cellular homeostasis of Aspergillus niger 103.
- Published
- 2023
- Full Text
- View/download PDF
23. Effects of organic fertilizer on soil properties and soil microorganisms.
- Author
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Tongbiao Li, Ruilin Wang, Jinjin Zhu, Lianbin Cao, Huili Xia, Mingcheng Wang, Yanan Guo, and Enzhong Li
- Subjects
ORGANIC fertilizers ,SOIL microbiology ,FERTILIZER application ,SOIL composition ,NUCLEOTIDE sequencing - Abstract
Organic fertilizers have been shown to positively impact soil microorganisms and the physical and chemical properties of soil. This study utilized high-throughput sequencing technology to analyze the composition and structure of the soil microbial community after organic fertilizer application. Enzyme activity, water content, and organic matter were also measured. The findings indicated that organic fertilizer application resulted in lower soil pH and reduced rates of miscellaneous bacteria, while increasing enzyme activity, water content, and organic matter. Moreover, the use of organic fertilizers improved alpha and beta diversity of soil microorganisms with Firmicutes experiencing a significant increase at the phylum level, and Bacillus, SBR1031, and Sphingomonas exhibiting higher relative abundance at the genus level. Conversely, levels of soil microorganisms like Nocardioides, Solidubrobacter, and Microvirga were lower in the organic fertilizer group compared to the control group. The application of PICRUST2 revealed significant up-regulation of PWY-6174 (mevalonate pathway II (haloarchaea)), ASPASN-PWY (super pathway of L-aspartate and L-asparagine biosynthesis), and PWY0-42 (2-methylcitrate cycle I) pathways in the organic fertilizer group, demonstrating the positive impact of organic fertilizers on microbial communities. Further analysis of the species composition of these pathways showed that in PWY-6174 pathway, Marine_ Group_ II in the control group displayed higher relative abundance than that in the organic fertilizer group (P < 0.05). In PWY0-42 pathways, the relative abundance of Rhodanobacter, Chujaibacter, Dyella, Luteimonas, and Thermomonas in organic fertilizer group was significantly higher than that in control group (P < 0.05). In ASPASN-PWY pathway, the relative abundance of Bacillus, Fonticella, Romboutsia, Chloroplast, and Tepidimicrobium in organic fertilizer group was significantly higher than that in control group (P < 0.05). In conclusion, this study indicated that organic fertilizers could effectively enhance the structure and composition of the soil microbial community, leading to improvements in the physical and chemical properties of soil. [ABSTRACT FROM AUTHOR]
- Published
- 2023
24. Extraction of
- Author
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Fujia, Chen, Yilin, Xu, Nana, Ding, Hongyan, Li, Tongbiao, Li, Fengyun, Liu, Mengxue, Liang, Li, Song, Junhe, Liu, Enzhong, Li, and Jiayang, Liu
- Subjects
Mice ,Cellulase ,Polysaccharides ,Temperature ,Animals ,Antioxidants ,Drugs, Chinese Herbal ,Hypolipidemic Agents - Abstract
This study focused on the optimization of ultrasound-assisted compound enzyme extraction for polysaccharides (RTPs) from
- Published
- 2022
25. Using Natural Biomacromolecules for Adsorptive and Enzymatic Removal of Aniline Blue from Water
- Author
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Xiaojuan You, Enzhong Li, Jiayang Liu, and Songhua Li
- Subjects
adsorption ,adsorbent ,laccase ,aniline blue ,dye removal ,Organic chemistry ,QD241-441 - Abstract
The present study investigated the adsorptive and enzymatic removal of aniline blue dye (AB) from aqueous solution using waxy riceprocessing waste (RW), peanut shell (PS), microbial waste of Aspergillus niger (MW) as low cost adsorbents, and laccase (Lac) as a biocatalyst. Commercial activated carbon (AC) was also employed to compare the adsorption performance with the three adsorbents. Dye removal was examined under various parameters in batch experiments. It was found that dye removal by RW and Lac was 89–94% noticeably better than that by MW and PS (20–70%). In any cases, AC produced the highest dye removal among the tested materials. The kinetics, isotherms, and thermodynamics were then analyzed to elucidate the adsorption process by the four adsorbents. The pseudo-second order kinetic was superior to the pseudo first order kinetic model in describing adsorption for all adsorbents. The Langmuir model fitted the adsorption process very well, indicating monolayer coverage of dyes on a solid surface. A thermodynamic analysis of enthalpy (ΔH°), entropy (ΔS°), and Gibbs free energy (ΔG°) classified the adsorption as a nonspontaneous and endothermic process. The results reveal diverse natural materials (e.g., processing waste RW) as novel substitutes for traditional activated carbon, as well as laccase as a green catalyst for the treatment of dye wastewater.
- Published
- 2018
- Full Text
- View/download PDF
26. Microstructure characteristics and mechanical properties of new-type FeNiCr laser cladding alloy coating on nodular cast iron
- Author
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Enzhong Li, Shixing Yan, Peng He, Binshi Xu, Yongjian Li, Shiyun Dong, and Xiaoting Liu
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Cladding (metalworking) ,0209 industrial biotechnology ,Materials science ,Scanning electron microscope ,Alloy ,Metals and Alloys ,02 engineering and technology ,engineering.material ,Microstructure ,Industrial and Manufacturing Engineering ,Computer Science Applications ,law.invention ,020303 mechanical engineering & transports ,020901 industrial engineering & automation ,0203 mechanical engineering ,Optical microscope ,law ,Transmission electron microscopy ,Modeling and Simulation ,Ultimate tensile strength ,Ceramics and Composites ,engineering ,Cast iron ,Composite material - Abstract
Nodular cast iron is a kind of traditional steel material which is widely used in many important industrial fields such as ship engine cylinders. But it is difficult to be repaired due to great cracking tendency during repairing process. It is difficult to find a kind of repairing material with an excellent combination of phase structure and mechanical properties. In the present research, a kind of new-type Fe-Ni-Cr alloy powder had been applied to the laser cladding process of nodular cast iron. The microstructure and phase evolution were determined and the mechanical properties had been also researched. Optical microscope (OM) and Scanning electron microscopy (SEM) were used to identify the microstructure. Energy dispersive microanalysis (EDS), X-ray diffraction (XRD) and Transmission electron microscope (TEM) were used to determine phase types of the claddings and interface zones. The results showed that the cladding layers illustrated good combination of hardness, tensile property and wear property.
- Published
- 2019
27. Deep pit repairing of nodular cast iron by laser cladding NiCu/Fe-36Ni low-expansion composite alloy
- Author
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Yongjian Li, Binshi Xu, Shixing Yan, Xiaoting Liu, Shiyun Dong, Peng He, and Enzhong Li
- Subjects
010302 applied physics ,Cladding (metalworking) ,Materials science ,Mechanical Engineering ,Alloy ,02 engineering and technology ,engineering.material ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,Microstructure ,01 natural sciences ,Brittleness ,Mechanics of Materials ,Residual stress ,0103 physical sciences ,Ultimate tensile strength ,engineering ,General Materials Science ,Cast iron ,Composite material ,0210 nano-technology ,Invar - Abstract
It is very easy to form hard and brittle phases (chilled structure and martensite) in the interface zone during the welding and cladding process of nodular cast iron which always decrease the tensile strength of the interface. Shrinkage during the freezing and cooling process of the weld and the clad also bring high residual stress which results in cracks in the interface. However, it is very difficult to eliminate hard and brittle phases in the interface. In this study, a new method was adopted to reduce the residual stress during the laser cladding process of nodular cast iron. That is, cladding alloy with low expansion characteristic was prepared to repair the nodular cast iron, and the shrinkage of the claddings could be reduced, which could reduce the residual stress and cracks. Fe-36Ni (Invar 36) alloy powders which show very low coefficient of expansion and NiCu alloy powders which could restrain the hard phases in the interface were used as the cladding powders to repair the pit of the nodular cast iron by designing composite claddings. The microstructure characteristics were identified by Optical microscope (OM) and Scanning electron microscopy (SEM). Chemical composition was conducted using Energy dispersive microanalysis (EDS). The results showed that no obvious cracks forming in the composite cladding layers and the interface. The average hardness of the cladding layer was about 190 HV and the tensile strength is beyond 460 MPa. The thermal expansion coefficient of the cladding layer is obviously lower than that of the substrate at temperatures below 230 °C and above 870 °C. The cracks were effectively controlled by using NiCu/Fe-36Ni low-expansion composite alloy during the deep pit repair of the nodular cast iron.
- Published
- 2019
28. Elimination of voids by laser remelting during laser cladding Ni based alloy on gray cast iron
- Author
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Shiyun Dong, Enzhong Li, Binshi Xu, Shixing Yan, Yongjian Li, Xiaoting Liu, and Peng He
- Subjects
010302 applied physics ,Cladding (metalworking) ,Heat-affected zone ,Materials science ,Scanning electron microscope ,Metallurgy ,Alloy ,02 engineering and technology ,engineering.material ,021001 nanoscience & nanotechnology ,Microstructure ,01 natural sciences ,Indentation hardness ,Atomic and Molecular Physics, and Optics ,Electronic, Optical and Magnetic Materials ,law.invention ,Optical microscope ,law ,0103 physical sciences ,engineering ,Cast iron ,Electrical and Electronic Engineering ,0210 nano-technology - Abstract
Gray cast iron was a traditional iron material which had been applied to industrial field for many years. For damaged gray cast iron, the repair process is very difficult because the gray cast iron was easy contaminated due to its special loose structure and the contaminants were difficult to remove. The strong tendency of the void formed in the interface region was a major trouble for the laser cladding process. In this paper, in order to eliminate those voids, laser cladding process on gray cast iron with remelting process was successfully carried out. The generation mechanism and elimination process of the voids were determined. The microstructure evolution in the melted zone (MZ), partially melted zone (PMZ) and heat affected zone (HAZ) was also researched. Optical microscopy (OM) and Scanning electron microscopy (SEM) were used to identify the microstructure and phase composition of the cladding layers and the transition zones. The results revealed that laser remelting was an effective way to eliminate the voids generated in the interface zone. The average microhardness of the hardened region formed during multi-layer cladding with remelting was about 600HV, and the width of the hardened region ranged from dozens of microns to hundreds of microns.
- Published
- 2019
29. Collaborative Learning- and Quiz-based Teaching Strategy in Biochemistry and Molecular Biology
- Author
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Enzhong Li, Yanli Yang, and Jie Zhong
- Subjects
Mathematics education ,Collaborative learning ,General Pharmacology, Toxicology and Pharmaceutics ,Psychology - Published
- 2019
30. Engineering O-glycosylation in modified N-linked oligosaccharide (Man12GlcNAc2∼Man16GlcNAc2) Pichia pastoris strains
- Author
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Jun Wu, Enzhong Li, Peng Sun, Xin Gong, Yuanhong Xu, Siqiang Li, Shaohong Chang, and Bo Liu
- Subjects
chemistry.chemical_classification ,Glycosylation ,biology ,Strain (chemistry) ,Chemistry ,General Chemical Engineering ,Mutant ,02 engineering and technology ,General Chemistry ,Protein degradation ,Oligosaccharide ,010402 general chemistry ,021001 nanoscience & nanotechnology ,biology.organism_classification ,01 natural sciences ,Yeast ,0104 chemical sciences ,Pichia pastoris ,carbohydrates (lipids) ,chemistry.chemical_compound ,Biochemistry ,0210 nano-technology ,Glycoprotein - Abstract
Yeast have been engineered for the production of therapeutic glycoproteins with humanized N-linked oligosaccharides. Both N- and O-linked oligosaccharides engineered yeast have been attractive prospects, since yeast-specific O-mannosylated proteins were reported to induce an aberrant immune response and alter pharmacokinetics in vivo. In the present study, we genetically manipulated O-glycosylation by disrupting O-mannosyltransferase PMT1 and PMT5 in a low-mannose type N-linked oligosaccharide (Man12GlcNAc2∼Man16GlcNAc2) engineered Pichia pastoris strain to produce therapeutic glycoproteins. The O-mannosyltransferase PMT1 mutant produces anti-Her-2 antibodies with reduced O-linked oligosaccharides and protein degradation, but this strain exhibited growth defects. However, the deletion of O-mannosyltransferase PMT5 individually has a minimal effect on O-glycosylation, degradation of the anti-Her-2 antibody, and strain growth. Thus, by disrupting O-mannosyltransferase PMT1 in an N-glycosylation engineered Pichia pastoris strain, we generated an effective glycoengineered Pichia pastoris strain to effectively produce therapeutic glycoproteins with both engineered N- and O-linked oligosaccharides.
- Published
- 2019
31. Therapeutic Potential of Indole Derivatives as Anti-HIV Agents: A Mini-review
- Author
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Chen Qingtai, Enzhong Li, Zhi Xu, Jinjin Zhu, and Chongchong Wu
- Subjects
Indole test ,Acquired Immunodeficiency Syndrome ,Protease ,Indoles ,biology ,business.industry ,Anti-HIV Agents ,medicine.medical_treatment ,HIV Infections ,General Medicine ,Disease ,Drug resistance ,medicine.disease ,Virology ,Reverse transcriptase ,Integrase ,Structure-Activity Relationship ,Acquired immunodeficiency syndrome (AIDS) ,Drug Discovery ,medicine ,biology.protein ,Delavirdine ,Humans ,business ,medicine.drug - Abstract
Acquired immunodeficiency syndrome (AIDS), caused by the human immunodeficiency virus (HIV), is one of the leading causes of human deaths. The advent of different anti-HIV drugs has turned AIDS/HIV from a deadly infection to chronic and manageable disease. However, the devel-opment of multidrug-resistant viruses, along with the severe side effects of anti-HIV agents, has com-promised their efficacy and limited the treatment options. Indoles, the most common frameworks in the bioactive molecules, represent attractive scaffolds for designing and developing novel drugs. In-dole derivatives are potent inhibitors of HIV enzymes such as reverse transcriptase, integrase, and protease. Furthermore, some indole-based agents, like delavirdine, have already been applied in clin-ics or are under clinical evaluations for the treatment of AIDS/HIV, revealing that indole moiety is a useful template for the development of anti-HIV agents. This review focuses on the recent advance-ment in indole derivatives, including indole alkaloids, hybrids, and dimers with anti-HIV potential, covering articles published between 2010 and 2020. The chemical structures, structure-activity rela-tionship, and mechanisms of action are also discussed.
- Published
- 2021
32. Metabolomics analysis reveals metabolic changes associated with trans-resveratrol treatment in experimental cryptorchidism mice
- Author
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Li Song, Mingguang Shao, Yun Li, Yuanhong Xu, Chaoying Liu, Chen Fujia, Enzhong Li, Baogen Wang, Yang Yurong, and Siqiang Li
- Subjects
Male ,Glycosylation ,Spermatogenesis arrest ,Reproductive technology ,Biology ,Andrology ,03 medical and health sciences ,chemistry.chemical_compound ,Mice ,0302 clinical medicine ,Endocrinology ,Metabolomics ,Biosynthesis ,Cryptorchidism ,Testis ,Genetics ,Metabolome ,Animals ,Spermatogenesis ,Molecular Biology ,030304 developmental biology ,0303 health sciences ,Uridine Diphosphate N-Acetylglucosamine ,Uridine diphosphate ,Metabolic pathway ,Reproductive Medicine ,chemistry ,Resveratrol ,030220 oncology & carcinogenesis ,Animal Science and Zoology ,Biomarkers ,Developmental Biology ,Biotechnology - Abstract
This study aimed to analyse global metabolomic changes associated with trans-resveratrol (RSV) treatment in mice with cryptorchidism using untargeted metabolomics. Cryptorchidism was established surgically in Kunming mice, which were then treated with 20µg g–1 day–1, s.c., RSV for 35 consecutive days. Typical manifestations of spermatogenesis arrest were seen in mice with cryptorchidism, and RSV treatment for 35 days restored spermatogenesis. Liquid chromatography–tandem mass spectrometry was used to profile the metabolome of testes from mice in the control (non-cryptorchid, untreated), cryptorchid and RSV-treated cryptorchid groups. In all, 1386 and 179 differential metabolites were detected in the positive and negative modes respectively. Seven and six potential biomarkers were screened for spermatogenesis arrest and restoration respectively. Pathway analysis showed changes in 197 metabolic pathways. The hexosamine biosynthesis pathway was inhibited in the cryptorchid group, which probably resulted in a decrease in the end product, uridine diphosphate N-acetylglucosamine (UDP-GlcNAc). Immunoblot analysis showed that total testicular protein O-linked β-N-acetylglucosamine glycosylation was related to spermatogenesis arrest, further indicating a decrease in UDP-GlcNAc in the cryptorchid group. Thus, untargeted metabolomics revealed the biochemical pathways associated with the restoration of metabolic status in the cryptorchid group following RSV treatment and the findings could be used to monitor the response to RSV treatment. This study provides a meaningful foundation for the future clinical application of RSV in the treatment of spermatogenesis dysfunction.
- Published
- 2020
33. Preparation of organic porous materials-TiO2/cu composite with excellent photocatalytic degradation performances toward degradation of organic pollutants in wastewater
- Author
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Enzhong Li, Ruonan Zhao, Tingting Liang, Qijie Xu, Wenbin Hu, Huibin Zhang, and Ning Zhang
- Subjects
Pollutant ,Materials science ,Polymers and Plastics ,Singlet oxygen ,Radical ,Organic Chemistry ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,chemistry.chemical_compound ,chemistry ,Chemical engineering ,Materials Chemistry ,Methyl orange ,Photocatalysis ,Phenol ,Degradation (geology) ,Hydroxyl radical ,0210 nano-technology - Abstract
Organic porous materials-TiO2/Cu composite (OPMTC) were readily fabricated through two steps, which included the formation of organic porous materials (OPM) and the in-situ synthesis of TiO2/Cu composite (TiO2/Cu) in the pore of OPM. Furthermore, the photocatalytic degradation behaviors of the photocatalysts toward organic pollutants were also investigated under simulated and nature sunlight, respectively. Finding showed that OPMTC allowed methyl orange, phenol, and methylene blue to be decomposed under simulated sunlight by the rate of 91.6%, 98.6%, and 100%, even to degraded DMF with a rate of 74% that revealed excellent photocatalytic degradation properties toward organic pollutants. Interestingly, OPMTC still kept the photocatalytic degradation ability after 6 time reuse. These results indicated the potential applications of OPMTC in the treatment of wastewater. Besides, the measurements of free radical intensities including superoxide free radical (O2·), singlet oxygen (1O2), and hydroxyl radical (·OH) of photocatalysts attributed to understanding the mechanism. The tested finding indicated that metallic copper doped TiO2 are beneficial to produce more free radicals thereby increasing photocatalytic degradation performances TiO2/Cu. OPMTC also had absorption ability measuring by BET that increased the contract area between photocatalyst and organic pollutants, thus, combining the two advantages thereby resulting in improving photocatalytic degradation properties.
- Published
- 2020
34. The Application of Diffusion- and Perfusion-Weighted Magnetic Resonance Imaging in the Diagnosis and Therapy of Acute Cerebral Infarction
- Author
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Ying Han, Enzhong Li, Jie Tian, Jian Chen, Huifang Wang, and Jianping Dai
- Subjects
Medical physics. Medical radiology. Nuclear medicine ,R895-920 ,Medical technology ,R855-855.5 - Abstract
Diffusion- and perfusion-weighted magnetic resonance imaging (DWI and PWI) was applied for stroke diagnose in 120 acute (
- Published
- 2006
- Full Text
- View/download PDF
35. Radiology research in mainland China in the past 10 years: a survey of original articles published in Radiology and European Radiology
- Author
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Jiaqian Xu, Guangming Lu, Enzhong Li, U. Joseph Schoepf, Zhen Lu Yang, Long Jiang Zhang, and Yun Fei Wang
- Subjects
Radiography, Abdominal ,Mainland China ,China ,medicine.medical_specialty ,Biomedical Research ,Bibliometric analysis ,Radiology, Interventional ,Subspecialty ,030218 nuclear medicine & medical imaging ,03 medical and health sciences ,0302 clinical medicine ,Humans ,Medicine ,Radiology, Nuclear Medicine and imaging ,Statistical analysis ,Retrospective Studies ,Neuroradiology ,medicine.diagnostic_test ,business.industry ,Interventional radiology ,General Medicine ,Mr imaging ,Bibliometrics ,030220 oncology & carcinogenesis ,Neuroradiography ,Radiology ,Periodicals as Topic ,business - Abstract
To evaluate the features and trends of Radiology research in Mainland China through bibliometric analysis of the original articles published in Radiology and European Radiology (ER) between 2006 and 2015. We reviewed the original articles published in Radiology and ER between 2006 and 2015. The following information was abstracted: imaging subspecialty, imaging technique(s) used, research type, sample size, study design, statistical analysis, study results, funding declarations, international collaborations, number of authors, department and province of the first author. All variables were examined longitudinally over time. Radiology research in Mainland China saw a substantial increase in original research articles published, especially in the last 5 years (P
- Published
- 2017
36. Improving the thermostability of GH11 xylanase XynXM from Aspergillus brunneoviolaceus CBS 621.78 by the design of cord region.
- Author
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Tongbiao Li, Jiaze Tang, Siqi Li, Hui Peng, YanYan Zhu, Jinjin Zhu, Yue Li, Chaoying Liu, and Enzhong Li
- Subjects
ENZYME stability ,SITE-specific mutagenesis ,PROTEIN-ligand interactions ,MOLECULAR interactions ,PROTEIN engineering ,XYLANASES - Abstract
Xylanases which hydrolyze ß-1,4-glycosidic bonds of xylans to yield xylooligosaccharides have a potential to be used in pulp bleaching, food processing, and feed processing. However, the poor thermostability of xylanases is a critical limiting factor in industrial applications. The thermostability of xylanases can be improved by protein engineering and computer-assisted design. In the present study, the thermostabilizing mutation G143W locating at the cord region of xylanase XynXM from Aspergillus brunneoviolaceus CBS 621.78 was predicted by the FireProt web server and constructed by site-directed mutagenesis. Heat tolerance experiments were performed in mutant G143W and wildtype XynXM. G143W exhibited a substantially improved thermostability with half-life of inactivation at 45°C enhanced from 41.5 min to 65.3 min, which was approximately 1.6-fold increase over that of wildtype. Furthermore, the optimum temperature was 50°C, which has the best reactive activity, and 5°C higher than that of XynXM (45°C). The reactive optimal pH of G143W increased from pH 5.0 to pH7.0. The pH stability span (4.0-9.0) of G143W displayed a significant improvement comparing to that of XynXM. Comparing to the wildtype, the half-life of G143W at 35°C in simulated intestinal fluid (pH 6.8, 10 mg/mL trypsin solution) was increased by 35.8 min. Novel hydrophobic contacts and hydrogen bonds were discovered in the model of G143W, which explained the reason of enhanced thermal stability. The key residues Trp57, Asn84, Val86, Tyr114, Gln117, Trp120, Glu127, Tyr137, Pro139, Ser141, Thr144, Ala161, Pro167, and Glu218 were identified in protein-ligand interaction by the molecular docking analysis using AutoDock Vina, which contributed to the catalytic activity and stability of enzyme. This study developed a thermostabilizing mutant G143W locating in the cord region of xylanase by the prediction of FireProt web server, which laid a foundation for exploring the functional relationship between cord region and xylanase XynXM. [ABSTRACT FROM AUTHOR]
- Published
- 2022
37. Isolation and identification of an acid-tolerant Lactobacillus species from chicken intestine and its application.
- Author
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Mingcheng Wang, Jiaze Tang, Gailing Wang, Yanan Guo, Lei Guo, and Enzhong Li
- Subjects
LACTOBACILLUS ,LACTIC acid bacteria ,POULTRY farms ,BROILER chickens ,REPRODUCTIVE isolation ,INTESTINES ,PLANT growth - Abstract
In order to promote the growth of broilers and improve disease resistance, lactic acid bacteria are often added to the diet during the breeding process. However, exogenous lactic acid bacteria are easy to break the balance of intestinal microbial flora, causing side effects. To obtain a safe Lactobacillus species to ensure its proper use in chicken farms, a strain of Lactobacillus with good acid tolerance was isolated and further purified from fresh chicken feces by using a Lactobacillus selective medium, peroxidase assay, coagulation assay, and acid tolerance screening. The strain was further identified as Lactobacillus sakei. The survival rate of this strain in a simulated gastrointestinal environment was 15.35%. The supplementation of the drinking water of broilers with 106 CFU/mL of Lactobacillus sakei JF-2 resulted in an 8.46% increase in body weight at farrowing, a 10.53% decrease in the feed-to-meat ratio, and a 26.49% increase in the European efficiency index, thus, achieving optimal economic benefits. This study showed that Lactobacillus sakei JF-2 could significantly promote the growth of broiler chickens and could be used as a broiler growth promoter. [ABSTRACT FROM AUTHOR]
- Published
- 2022
38. Rapid improvement in thermostability of GH11 xylanase (XynASP) from Aspergillus saccharolyticus JOP 1030-1 by tryptophan residue substitution.
- Author
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Tongbiao Li, Xiaoli Zhu, Mingyang Ye, Mingcheng Wang, Jinjin Zhu, Fujia Shen, and Enzhong Li
- Subjects
XYLANASES ,ORGANIC solvents ,INDUSTRIAL enzymology ,TRYPTOPHAN ,SITE-specific mutagenesis ,PEPTIDES - Abstract
Poor thermal stability is the main limiting factor for the industrial application of xylanase. To explore the way to improve the thermal stability of xylanase and obtain thermostable xylanase, a mesophilic xylanase gene (xynASP) (GenBank accession number: XM_025579666.1) derived from Aspergillus saccharolyticus JOP 1030-1 was mined by using the BLAST program and Thermomonospora fusca xylanase gene (TfxA) (GenBank accession number: KF927166.1) as the probe. The mined gene without signal peptide was cloned into Escherichia coli BL21 (DE3) cells by using the pET-28a expression system. Enzymatic characterization and conformation analysis showed that the recombinant XynASP belonged to GH11 family mesophilic xylanase with poor heat resistance, which limits the industrial applications of the enzyme at high temperature conditions. To rapidly enhance thermostability, the aromatic amino acids tryptophan was introduced at the 41st site of N-terminal of recombinant XynASP to design the variant T41W. The results experimentally confirmed that the beneficial variant T41W displayed the optimum temperature increased by 10°C comparing to that of the wild type XynASP. The half-life of inactivation of the variant T41W increased to 127.7 min at 45°C, which was approximately 6.2-fold longer than that of the wild type and exhibited a substantially improved thermostability. In addition, the variant T41W exhibited a high stability after incubated with metal ions and different concentrations of various organic solvents (methanol, ethanol, and isopropanol), which was similar to that of the wild type XynASP. This study demonstrated that the enzyme could be rapidly designed and substantially improved thermostability by site-directed mutagenesis. The resulted variant T41W could be a prospective candidate for industrial application. [ABSTRACT FROM AUTHOR]
- Published
- 2022
39. Collaborative Testing in Practical Laboratories: An Effective Teaching–Learning Method in Histology
- Author
-
Enzhong Li and Yuping Guo
- Subjects
China ,Educational measurement ,Histology ,020205 medical informatics ,education ,02 engineering and technology ,Final examination ,Education ,Random Allocation ,Teaching styles ,Pedagogy ,0202 electrical engineering, electronic engineering, information engineering ,Learning ,Medicine ,Cooperative Behavior ,Student learning ,Random allocation ,Medical education ,General Veterinary ,business.industry ,05 social sciences ,050301 education ,Questionnaire ,General Medicine ,Achievement ,Attitude ,Learning methods ,Educational Measurement ,Education, Veterinary ,business ,0503 education ,Effective teaching - Abstract
This article presents an experimental teaching and learning program used in histology with first-year students in the second term in the Faculty of Biology at Huanghuai University, China. Eighty-six students were divided randomly into two groups (n=43 per group). Tests were conducted at the end of each practical laboratory (10 laboratories in total) in which collaborative testing was used in the experimental group and traditional testing in the control group. To assess achievement, a final examination in histology was carried out at the end of the course. To determine students' attitude to the teaching styles, a questionnaire survey was conducted at the end of the term. Results showed that students preferred the collaborative testing format. In the experimental group, students' scores were significantly higher than those of students in the control group in final examinations. These findings indicate that collaborative testing enhances student learning and understanding of the material taught, and suggest that collaborative testing is an effective teaching–learning method in histology.
- Published
- 2016
40. Engineering
- Author
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Siqiang, Li, Peng, Sun, Xin, Gong, Shaohong, Chang, Enzhong, Li, Yuanhong, Xu, Jun, Wu, and Bo, Liu
- Abstract
Yeast have been engineered for the production of therapeutic glycoproteins with humanized
- Published
- 2018
41. Using Natural Biomacromolecules for Adsorptive and Enzymatic Removal of Aniline Blue from Water
- Author
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Enzhong Li, Xiaojuan You, Jiayang Liu, and Songhua Li
- Subjects
Entropy ,Enthalpy ,Pharmaceutical Science ,02 engineering and technology ,010501 environmental sciences ,aniline blue ,01 natural sciences ,Endothermic process ,Article ,Analytical Chemistry ,Catalysis ,laccase ,lcsh:QD241-441 ,symbols.namesake ,adsorbent ,Adsorption ,lcsh:Organic chemistry ,Drug Discovery ,medicine ,Physical and Theoretical Chemistry ,Coloring Agents ,0105 earth and related environmental sciences ,Aniline Compounds ,Aqueous solution ,Chemistry ,Organic Chemistry ,Temperature ,Water ,Langmuir adsorption model ,021001 nanoscience & nanotechnology ,Gibbs free energy ,dye removal ,Kinetics ,Chemistry (miscellaneous) ,adsorption ,Charcoal ,symbols ,Thermodynamics ,Molecular Medicine ,0210 nano-technology ,Water Pollutants, Chemical ,Activated carbon ,medicine.drug ,Nuclear chemistry - Abstract
The present study investigated the adsorptive and enzymatic removal of aniline blue dye (AB) from aqueous solution using waxy riceprocessing waste (RW), peanut shell (PS), microbial waste of Aspergillus niger (MW) as low cost adsorbents, and laccase (Lac) as a biocatalyst. Commercial activated carbon (AC) was also employed to compare the adsorption performance with the three adsorbents. Dye removal was examined under various parameters in batch experiments. It was found that dye removal by RW and Lac was 89&ndash, 94% noticeably better than that by MW and PS (20&ndash, 70%). In any cases, AC produced the highest dye removal among the tested materials. The kinetics, isotherms, and thermodynamics were then analyzed to elucidate the adsorption process by the four adsorbents. The pseudo-second order kinetic was superior to the pseudo first order kinetic model in describing adsorption for all adsorbents. The Langmuir model fitted the adsorption process very well, indicating monolayer coverage of dyes on a solid surface. A thermodynamic analysis of enthalpy (&Delta, H°, ), entropy (&Delta, S°, ), and Gibbs free energy (&Delta, G°, ) classified the adsorption as a nonspontaneous and endothermic process. The results reveal diverse natural materials (e.g., processing waste RW) as novel substitutes for traditional activated carbon, as well as laccase as a green catalyst for the treatment of dye wastewater.
- Published
- 2018
- Full Text
- View/download PDF
42. Simulation of vaporization in low fluence nanosecond laser ablation of aluminum alloy
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Shixing Yan, Peng He, Chaoqun Song, Binshi Xu, Enzhong Li, and Shiyun Dong
- Subjects
Liquid metal ,Microscope ,Materials science ,Laser ablation ,Buoyancy ,Laser scanning ,medicine.medical_treatment ,Physics::Medical Physics ,engineering.material ,Ablation ,law.invention ,Impact crater ,law ,Vaporization ,medicine ,engineering ,Physics::Atomic Physics ,Composite material - Abstract
This paper presents a multi-phase flow model for the nanosecond laser ablation of aluminum alloy at a low fluence based on finite volume method, considering gravity, recoil pressure, buoyancy and surface tension to describe vaporization. Actual morphology of ablation crater was measured by a laser scanning confocal microscope to verify the model. Results show that vaporization is the main ablation mechanism for 100ns laser ablation at low fluences, and the peak temperature is only 50% of critical temperature. Both the experimental and calculated crater have a wall-like bulge around the rim, as a result of impact of recoil pressure and resolidification of pushed liquid metal. The calculated depth and diameter of crater are in good agreement with the corresponding experimental measurement indicating the feasibility of the model.
- Published
- 2018
43. Functional Neuroimaging and Its Applications to Critical Brain Diseases
- Author
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JiaHong Gao, GuangMing Lu, YuHua Peng, ErDan Dong, and EnZhong Li
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Cognitive science ,Functional neuroimaging ,Translational medicine ,Pharmacology (medical) ,Brain research ,Psychology ,Neuroscience ,Brain disease - Abstract
This review provides an overview of current status and developing trends for technology and methodology used in functional neuroimaging, introduces the latest and significant findings in brain research and challenges in translational medicine based on imaging techniques, and proposes future research directions and major scientific questions to be addressed in the field of functional neuroimaging and its applications to critical brain diseases.
- Published
- 2015
44. Effect of resveratrol on restoring spermatogenesis in experimental cryptorchid mice and analysis of related differentially expressed proteins
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Enzhong Li, Yuping Guo, Gailing Wang, Qingwang Li, and Fujia Chen
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Serum testosterone ,medicine.medical_specialty ,Serum fsh ,Significant difference ,Cell Biology ,General Medicine ,Biology ,Resveratrol ,Sperm ,chemistry.chemical_compound ,Endocrinology ,chemistry ,Internal medicine ,medicine ,Spermatogenesis ,Sperm counts ,Testosterone - Abstract
The present study aimed to evaluate the effect of trans-Resveratrol on spermatogenesis. Male Kunming suckling mice (10 days old) were surgically rendered cryptorchid and subcutaneously injected with trans-Resveratrol at doses of 5, 10, 20, and 40 µg/g/day as groups I, II, III, and IV, respectively, for 35 days. Animals in the control group received 10 µL/mouse/day of olive oil. Serum estradiol, testosterone, FSH, and LH levels were measured on day 45. Tissue analysis and sperm morphological abnormalities analysis were done. Results showed that in the control group and group I only spermatogonia and primary spermatocytes were present, whereas spermatogenesis was totally restored in groups II, III, and IV. Sperm counts in groups III and IV were remarkably higher than the control group (P
- Published
- 2015
45. Isolation and identification of Sphingomonas sp. from chicken cecum and its ammonia-degrading activity.
- Author
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Mingcheng Wang, Ye Wang, Gailing Wang, Linglong Xu, and Enzhong Li
- Subjects
SPHINGOMONAS ,POULTRY manure ,CECUM ,ODORS ,NEWCASTLE disease virus ,CHICKENS ,CONJUNCTIVA - Abstract
Emission of ammonia is one of the main causes for the bad odor of the chicken manure. The ammonia does not only impose threats on animal health but also is associated with stimulations to chicken mucous membrane, such as conjunctiva and cornea. The mucous membrane damage increases chicken susceptibility to pathogenic microorganisms, for example the Newcastle disease virus and mycoplasma, etc., and eventually results in reduced chicken production. In this study, one bacterial strain degrading ammonia was isolated from the chicken cecum with the use of anaerobic nitrification medium and aerobic denitrification medium. This strain was yellow-colored, Gram-stain-negative, and strictly aerobic. Phylogenetic analysis based on 16S rRNA gene sequence indicated that the bacterial strain is a member of the genus of Sphingomonas, designated Sphingomonas sp. Z392. Afterwards, Sphingomonas sp. Z392 was fed to the chicken in water with a concentration of 0.4x106 CFU/mL. After feeding, the organic nitrogen, ammonium nitrogen, and nitrate nitrogen contents in chicken manure were determined by the Kjeldahl method, the indophenol blue method, and the phenol-disulfonic acid method, respectively. The results showed that the content of organic nitrogen in chicken manure decreased significantly (P<0.05) after addition of Z392 into the feeding water for 10 days, while the content of ammonium nitrogen in fresh chicken manure increased at first but decreased significantly after 24 h (P<0.05) and the content of nitrate nitrogen increased significantly (P<0.05). The research indicated that the addition of Sphingomonas sp. into chicken feeding water had obvious effects on degrading the ammonia, deodorizing the manure, and purifying the environment. [ABSTRACT FROM AUTHOR]
- Published
- 2021
46. Polysaccharides from
- Author
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Fujia, Chen, Dahong, Li, Hongqi, Shen, Chunhong, Wang, Enzhong, Li, Huihui, Xing, Li, Guo, Qingchun, Zhao, Junhao, Shi, Hoang, Nguyen, and Jiayang, Liu
- Subjects
Hot Temperature ,Cellulase ,Ultrasonic Waves ,Polysaccharides ,Trichosanthes ,Research Article - Abstract
An efficient procedure for ultrasound-assisted enzymatic extraction of crude polysaccharides from Trichosanthes Fructus (crude TFP) using response surface methodology (RSM) was developed. The Box–Behnken design was applied to optimize the effects of pH (X1), enzyme amount (X2), extraction temperature (X3), and liquid-to-solid ratio (X4) on the extraction. The statistical analysis indicated that the independent variables (X4, X2, and X3), the quadratic coefficients (X12, X22, X32, and X42), and the interaction coefficient (X1X3) had significant impact on the yield of crude TFP. The optimal conditions were determined as follows: pH 4.5, enzyme amount 5000 u/g, extraction temperature 45°C, and liquid-to-solid ratio 30 ml/g. The experimental yield of crude TFP was 6.58%, which was very close to the predicted yield of 6.71%. TFPI was then purified and characterized with Sephadex G-100 column, UV-Vis, GPC, and FT-IR. The average molecular weight of TFPI was calculated to be 1.49 × 105 Da. TFPI exhibited strong reducing power and possessed not only remarkable scavenging activities against ABTS•+ and DPPH radicals, but also high antitumor activities in C4-2, DU145, and PC3 cells. The results suggest that Trichosanthes Fructus and TFPI could be a novel potent natural medicine with antioxidant and antitumor activities.
- Published
- 2017
47. Erratum: Adsorption of methylene blue on an agro-waste oiltea shell with and without fungal treatment
- Author
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Jiayang Liu, Enzhong Li, Xiaojuan You, Changwei Hu, and Qingguo Huang
- Subjects
Methylene Blue ,Kinetics ,Multidisciplinary ,Models, Chemical ,Microscopy, Electron, Scanning ,Thiazines ,Industrial Waste ,Plant Oils ,Water ,Adsorption ,Erratum ,Hydrogen-Ion Concentration ,Lignin - Abstract
Scientific Reports 6: Article number: 38450; published online: 05 December 2016; updated: 16 March 2017 In this Article, Enzhong Li is incorrectly listed as being affiliated with ‘Shandong Provincial Key Laboratory of Water and Soil Conservation and Environmental Protection, Linyi University, Linyi,276000, China’ The correct affiliation is listed below
- Published
- 2017
48. Adsorption of methylene blue on an agro-waste oiltea shell with and without fungal treatment
- Author
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Jiayang Liu, Enzhong Li, Changwei Hu, Xiaojuan You, and Qingguo Huang
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Multidisciplinary ,Aqueous solution ,Sorbent ,biology ,Langmuir adsorption model ,Sorption ,02 engineering and technology ,010501 environmental sciences ,021001 nanoscience & nanotechnology ,biology.organism_classification ,01 natural sciences ,Article ,chemistry.chemical_compound ,symbols.namesake ,Adsorption ,chemistry ,symbols ,Fourier transform infrared spectroscopy ,0210 nano-technology ,Methylene blue ,0105 earth and related environmental sciences ,Nuclear chemistry ,Trametes versicolor - Abstract
A lignocellulosic waste oiltea shell (OTS) was evaluated as an inexpensive sorbent to remove methylene blue (MB) from aqueous solution. Fungal treatment of OTS increased the MB adsorption by modifying the physicochemical properties of OTS and simultaneously produced laccase as a beneficial co-product. Without fungal treatment, the maximum amount of adsorption (qm) of MB by OTS was 64.4 mg/g, whereas the treatment with fungus Pycnoporus sp. and Trametes versicolor increased qm up to 72.5 mg/g and 85.7 mg/g, respectively. This is because of the improved surface area and pore sizes as well as altered chemical compositions. The equilibrium sorption data for OTS both with and without treatment fitted to the Langmuir model, and the sorption rate data well fitted to the pseudo second-order kinetic model. The changes in free energy (ΔG°) and separation factor (RL) indicated that the sorption was spontaneous and favorable. Scanning electron microscopy and Fourier transform infrared spectroscopy showed the changes in the surface morphology and functional groups of OTS after fungal treatment. The agro-waste OTS could be utilized as a low-cost adsorbent for efficient dye removal, and fungal treatment can serve as a mild and clean technique to increase the adsorptive capacity of OTS.
- Published
- 2016
49. Isolation, identification, and determination of dominant desulfurizing bacterial strains from chicken manure.
- Author
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Gailing Wang, Mingcheng Wang, Tongbiao Li, Changli Liang, and Enzhong Li
- Subjects
POULTRY manure ,SULFUR bacteria ,HYDROGEN sulfide ,POLLUTION ,CHICKEN coops - Abstract
Hydrogen sulfide is a colorless, highly toxic, water-soluble acidic gas. When the concentration in a chicken house exceeds the tolerance range of chicken, hydrogen sulfide will stimulate their mucosa and damage their sense of smell. It can also reduce the chicken’s appetite, growth performance, and resistance to disease. This study was aimed to separate desulfurizing bacterial strains from fresh chicken manure to reduce the impact of hydrogen sulfide on chickens, humans, and environmental pollution more generally. With the methods of enrichment culture, plate separation, and barium chloride screening, four desulfurizing bacterial strains (L
1 , L2 , L3 , and L4 ) were isolated from chicken manure. They were identified as Providencia sp., Arthrobacter AMP-5, Arthrobacter AMP-6, and Thiobacillus sp. by 16S rRNA sequencing. The desulfurization effects of these bacteria were investigated. The results showed that the desulfurization rate of Thiobacillus sp. reached 35.3%, which is the highest among the four strains and suggested that Thiobacillus sp. might be an effective sulfur bacterium degrading hydrogen sulfide. [ABSTRACT FROM AUTHOR]- Published
- 2020
50. The Application of Diffusion- and Perfusion-Weighted Magnetic Resonance Imaging in the Diagnosis and Therapy of Acute Cerebral Infarction
- Author
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Huifang Wang, Jianping Dai, Ying Han, Enzhong Li, Jian Chen, and Jie Tian
- Subjects
lcsh:Medical physics. Medical radiology. Nuclear medicine ,lcsh:Medical technology ,medicine.diagnostic_test ,business.industry ,lcsh:R895-920 ,Penumbra ,Infarction ,Magnetic resonance imaging ,Sequela ,Perfusion-Weighted Magnetic Resonance Imaging ,medicine.disease ,Article ,lcsh:R855-855.5 ,Cerebral blood flow ,medicine ,Effective diffusion coefficient ,Radiology, Nuclear Medicine and imaging ,cardiovascular diseases ,business ,Nuclear medicine ,Stroke - Abstract
Diffusion- and perfusion-weighted magnetic resonance imaging (DWI and PWI) was applied for stroke diagnose in 120 acute (<48h) ischemic stroke patients. At hyperacute (<6h) stage, it is difficult to find out the infarction zone in conventional T1 or T2 image, but it is easy in DWI, apparent diffusion coefficient (ADC) map; when at 3–6-hour stage it is also easy in PWI, cerebral blood flow (CBF) map, cerebral blood volume (CBV) map, and mean transit time (MTT) map; at acute (6–48 h) stage, DWI or PWI is more sensitive than conventional T1 or T2 image too. Combining DWI with ADC, acute and chronic infarction can be distinguished. Besides, penumbra which should be developed in meaning was used as an indication or to evaluate the therapeutic efficacy. There were two cases (<1.5h) that broke the model of penumbra because abnormity was found in DWI but not that in PWI, finally they recovered without any sequela.
- Published
- 2006
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