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5 results on '"Envelope E"'

1. Multi-epitope peptide vaccines targeting dengue virus serotype 2 created via immunoinformatic analysis

Author

Radwa N. Morgan, Nasser S. M. Ismail, Mohammad Y. Alshahrani, and Khaled M. Aboshanab

Subjects
Dengue viruses, DENV-2, Nonstructural protein 1 (NS1), Envelope E, Peptide vaccine, Epitopes, Medicine, Science
Abstract

Abstract The Middle East has witnessed a greater spread of infectious Dengue viruses, with serotype 2 (DENV-2) being the most prevalent form. Through this work, multi-epitope peptide vaccines against DENV-2 that target E and nonstructural (NS1) proteins were generated through an immunoinformatic approach. MHC class I and II and LBL epitopes among NS1 and envelope E proteins sequences were predicted and their antigenicity, toxicity, and allergenicity were investigated. Studies of the population coverage denoted the high prevalence of NS1 and envelope-E epitopes among different countries where DENV-2 endemic. Further, both the CTL and HTL epitopes retrieved from NS1 epitopes exhibited high conservancies’ percentages with other DENV serotypes (1, 3, and 4). Three vaccine constructs were created and the expected immune responses for the constructs were estimated using C-IMMSIM and HADDOCK (against TLR 2,3,4,5, and 7). Molecular dynamics simulation for vaccine construct 2 with TLR4 denoted high binding affinity and stability of the construct with the receptor which might foretell favorable in vivo interaction and immune responses.

Published
2024
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2. Multi-epitope peptide vaccines targeting dengue virus serotype 2 created via immunoinformatic analysis.

Author

Morgan, Radwa N., Ismail, Nasser S. M., Alshahrani, Mohammad Y., and Aboshanab, Khaled M.

Subjects
*PEPTIDE vaccines, *MOLECULAR dynamics, *AMINO acid sequence, *EPITOPES, *IMMUNE response, *DENGUE viruses
Abstract

The Middle East has witnessed a greater spread of infectious Dengue viruses, with serotype 2 (DENV-2) being the most prevalent form. Through this work, multi-epitope peptide vaccines against DENV-2 that target E and nonstructural (NS1) proteins were generated through an immunoinformatic approach. MHC class I and II and LBL epitopes among NS1 and envelope E proteins sequences were predicted and their antigenicity, toxicity, and allergenicity were investigated. Studies of the population coverage denoted the high prevalence of NS1 and envelope-E epitopes among different countries where DENV-2 endemic. Further, both the CTL and HTL epitopes retrieved from NS1 epitopes exhibited high conservancies' percentages with other DENV serotypes (1, 3, and 4). Three vaccine constructs were created and the expected immune responses for the constructs were estimated using C-IMMSIM and HADDOCK (against TLR 2,3,4,5, and 7). Molecular dynamics simulation for vaccine construct 2 with TLR4 denoted high binding affinity and stability of the construct with the receptor which might foretell favorable in vivo interaction and immune responses. [ABSTRACT FROM AUTHOR]

Published
2024
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3. Multi-laboratory comparison of three commercially available Zika IgM enzyme-linked immunosorbent assays.

Author

Basile, Alison Jane, Goodman, Christin, Horiuchi, Kalanthe, Sloan, Angela, Johnson, Barbara W., Kosoy, Olga, Laven, Janeen, Panella, Amanda J., Sheets, Isabel, Medina, Freddy, Mendoza, Emelissa J., Epperson, Monica, Maniatis, Panagiotis, Semenova, Vera, Steward-Clark, Evelene, Wong, Emily, Biggerstaff, Brad J., Lanciotti, Robert, Drebot, Michael, and Safronetz, David

Subjects
*ZIKA virus, *IMMUNOGLOBULIN M, *ENZYME-linked immunosorbent assay, *VIRAL nonstructural proteins, *HORSERADISH peroxidase, *VIRAL antigens
Published
2018
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4. Multi-laboratory comparison of three commercially available Zika IgM enzyme-linked immunosorbent assays

Author

Michael A. Drebot, Evelene Steward-Clark, Brad J. Biggerstaff, Olga Kosoy, Janeen Laven, Freddy A. Medina, Amanda J. Panella, Monica Epperson, Kalanthe Horiuchi, Barbara W. Johnson, David Safronetz, Emelissa J Mendoza, Alison Jane Basile, Emily Wong, Angela Sloan, Panagiotis Maniatis, Jarad Schiffer, Robert S. Lanciotti, Christin H. Goodman, Vera A. Semenova, and Isabel Sheets

Subjects
0301 basic medicine, Male, IgM, IgG, 030106 microbiology, NS1, Enzyme-Linked Immunosorbent Assay, Comparison, Viral Nonstructural Proteins, Antibodies, Viral, Sensitivity and Specificity, Immunoglobulin G, Virus, Article, Dengue fever, Dengue, 03 medical and health sciences, Zika, Virology, Chlorocebus aethiops, medicine, Animals, Humans, Vero Cells, chemistry.chemical_classification, biology, Zika Virus Infection, Commercial, Zika Virus, medicine.disease, Nonstructural protein 1, Enzyme, chemistry, Kits, Immunoglobulin M, Envelope E, biology.protein, ELISA, Female
Published
2018

5. Identification of Effective siRNA Blocking the Expression of SARS Viral Envelope E and RDRP Genes

Author

Yinghe Hu, Shi Meng, Changxiu Cao, Bo Meng, and Yue-woon Lui

Subjects
Gene Expression Regulation, Viral, viruses, Bioengineering, Biology, Applied Microbiology and Biotechnology, Biochemistry, Viroporin Proteins, Small hairpin RNA, Mice, chemistry.chemical_compound, Viral Envelope Proteins, Viral envelope, RNA interference, RNA polymerase, Gene expression, Animals, RNA, Small Interfering, Molecular Biology, SARS, Regulation of gene expression, Expression vector, Reverse Transcriptase Polymerase Chain Reaction, Research, RNA, RNA-Dependent RNA Polymerase, Molecular biology, Mutagenesis, Insertional, Severe acute respiratory syndrome-related coronavirus, chemistry, envelope E, RNAi, NIH 3T3 Cells, RNA Interference, RNA-dependent RNA polymerase (RDRP), Biotechnology
Abstract

A cell-based assay was developed to screen small interference RNA (siRNA) to block the expression of two genes of the severe acute respiratory syndrome (SARS) virus. These two genes encode RNA-dependent RNA polymerase (RDRP) and envelope E protein. The RDRP plays an essential role in viral RNA replication where envelope E protein is involved in envelope formation and virus assembly. The RDRP and envelope E genes, based on published sequences, have been synthesized and cloned into mammalian expression vectors. In addition, four siRNA sites for the RDRP gene and two siRNA sites for envelope E gene were designed and tested. The siRNA or short hairpin RNA (shRNA) expression cassettes were co-transfected with the SARS viral RDRP or envelope E expression vectors into NIH 3T3 cells. The expression levels of RDRP and envelope E genes were examined by reverse transcription followed by quantitative real-time polymerase chain reaction (PCR). Two of the siRNA expression cassettes for RDRP successfully inhibited the expression of the gene, whereas both of the siRNA expression cassettes for envelope E decreased approx 90% of the envelope E gene expression. The siRNA and shRNA for one of the siRNA sites of the RDRP gene were also tested, and it was found that both inhibited exogenous RDRP expression in a dose-dependent manner. These siRNA molecules could be used to examine the function of these genes in SARS virus replication and assembly. Furthermore, these molecules could potentially be developed into therapeutic agents for the treatment of patients with SARS.

Published
2006
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