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484 results on '"Engel U"'

1. Automated image analysis in the study of collagenous colitis

Author

Fiehn AM, Kristensson M, Engel U, Munck LK, Holck S, and Engel PJH

Subjects
Microscopic colitis, collagenous colitis, collagenous colitis incomplete, automated image analysis, Diseases of the digestive system. Gastroenterology, RC799-869
Abstract

Anne-Marie Kanstrup Fiehn,1,2 Martin Kristensson,3 Ulla Engel,4 Lars Kristian Munck,5,6 Susanne Holck,4 Peter Johan Heiberg Engel1,6 1Department of Pathology, Roskilde Hospital, Roskilde, 2Department of Pathology, Copenhagen University Hospital, Rigshospitalet, Copenhagen, 3Digital Pathology, Visiopharm, Hørsholm, 4Department of Pathology, Copenhagen University Hospital, Hvidovre, 5Department of Gastroenterology, Koege Hospital, Koege, 6Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark Purpose: The aim of this study was to develop an automated image analysis software to measure the thickness of the subepithelial collagenous band in colon biopsies with collagenous colitis (CC) and incomplete CC (CCi). The software measures the thickness of the collagenous band on microscopic slides stained with Van Gieson (VG). Patients and methods: A training set consisting of ten biopsies diagnosed as CC, CCi, and normal colon mucosa was used to develop the automated image analysis (VG app) to match the assessment by a pathologist. The study set consisted of biopsies from 75 patients. Twenty-five cases were primarily diagnosed as CC, 25 as CCi, and 25 as normal or near-normal colonic mucosa. Four pathologists individually reassessed the biopsies and categorized all into one of the abovementioned three categories. The result of the VG app was correlated with the diagnosis provided by the four pathologists. Results: The interobserver agreement for each pair of pathologists ranged from Κ-values of 0.56–0.81, while the Κ-value for the VG app vs each of the pathologists varied from 0.63 to 0.79. The overall agreement between the four pathologists was Κ=0.69, while the overall agreement between the four pathologists and the VG app was Κ=0.71. Conclusion: In conclusion, the Visiopharm VG app is able to measure the thickness of a subepithelial collagenous band in colon biopsies with an accuracy comparable to the performance of a pathologist and thereby provides a promising supplementary tool for the diagnosis of CC and CCi and in particular for research. Keywords: microscopic colitis, collagenous colitis, incomplete collagenous colitis, automated image analysis

Published
2016

2. Allgemeinchirurgie und Viszeralchirurgie

Author

Liehn, M., Steinmüller, L., Bröker, S., Engel, U., Schimmelpenning, H., Weise, R., Preuth, M., Liehn, Margret, editor, Lengersdorf, Brigitte, editor, Steinmüller, Lutz, editor, and Döhler, Rüdiger, editor

Published
2016
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3. Actin-rich lamellipodia-like protrusions contribute to the integrity of epithelial cell–cell junctions

Author

Senju, Y. (Yosuke), Mushtaq, T. (Toiba), Vihinen, H. (Helena), Manninen, A. (Aki), Saarikangas, J. (Juha), Ven, K. (Katharina), Engel, U. (Ulrike), Varjosalo, M. (Markku), Jokitalo, E. (Eija), Lappalainen, P. (Pekka), Senju, Y. (Yosuke), Mushtaq, T. (Toiba), Vihinen, H. (Helena), Manninen, A. (Aki), Saarikangas, J. (Juha), Ven, K. (Katharina), Engel, U. (Ulrike), Varjosalo, M. (Markku), Jokitalo, E. (Eija), and Lappalainen, P. (Pekka)

Abstract

Metastasis-suppressor 1 (MTSS1) is a membrane-interacting scaffolding protein that regulates the integrity of epithelial cell–cell junctions and functions as a tumor suppressor in a wide range of carcinomas. MTSS1 binds phosphoinositide-rich membranes through its I-BAR domain and is capable of sensing and generating negative membrane curvature in vitro. However, the mechanisms by which MTSS1 localizes to intercellular junctions in epithelial cells and contributes to their integrity and maintenance have remained elusive. By carrying out EM and live-cell imaging on cultured Madin-Darby canine kidney cell monolayers, we provide evidence that adherens junctions of epithelial cells harbor lamellipodia-like, dynamic actin-driven membrane folds, which exhibit high negative membrane curvature at their distal edges. BioID proteomics and imaging experiments demonstrated that MTSS1 associates with an Arp2/3 complex activator, the WAVE-2 complex, in dynamic actin-rich protrusions at cell–cell junctions. Inhibition of Arp2/3 or WAVE-2 suppressed actin filament assembly at adherens junctions, decreased the dynamics of junctional membrane protrusions, and led to defects in epithelial integrity. Together, these results support a model in which membrane-associated MTSS1, together with the WAVE-2 and Arp2/3 complexes, promotes the formation of dynamic lamellipodia-like actin protrusions that contribute to the integrity of cell–cell junctions in epithelial monolayers.

Published
2023

5. Measurement and Test Techniques

Author

Abele, E., Aurich, J. C., Behrens, B. -A., Biermann, D., Brecher, C., Brinksmeier, E., Czora, M., Denkena, B., Engel, U., Großmann, K., Heisel, U., Heinisch, D., Hermes, R., Kirsch, B., Klocke, F., Krause, A., Kroiß, T., Laurischkat, R., Löser, M., Mahr, F., Meier, H., Pischan, M., Rasper, P., Scheidler, A. V., Storchak, M., Uhlmann, E., Weiß, M., Denkena, Berend, editor, and Hollmann, Ferdinand, editor

Published
2013
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11. Analyzing data streams for social scientists

Author

Ippel, L., Kaptein, Maurits, Vermunt, Jeroen, Engel, U., Quan-Haase, A., Xun-Liu, S., Lyberg, L., Data Analytics, and Department of Methodology and Statistics

Published
2022

16. Genital Tract GAS Infection ISIDOG Guidelines

Author

Donders, G, Greenhouse, P, Donders, F, Engel, U, Paavonen, J, Mendling, W, HUS Gynecology and Obstetrics, Clinicum, and Department of Obstetrics and Gynecology

Subjects
endometritis, HEMOLYTIC STREPTOCOCCI, Streptococcus pyogenes, maternal mortality, CLINICAL-MANIFESTATIONS, invasive GAS infection, ERYTHROMYCIN RESISTANCE, GROUP-A STREPTOCOCCUS, CHRONIC PHARYNGEAL CARRIAGE, GROUP-B STREPTOCOCCUS, sepsis, puerperal infection, INTERNALIZATION-ASSOCIATED GENE, 3123 Gynaecology and paediatrics, VULVO-VAGINITIS, FIBRONECTIN-BINDING PROTEIN, TOXIC-SHOCK-SYNDROME
Abstract

There has been an increasing worldwide incidence of invasive group A streptococcal (GAS) disease in pregnancy and in the puerperal period over the past 30 years. Postpartum Group A streptococci infection, and in particular streptococcal toxic shock syndrome (TSS) and necrotizing fasciitis, can be life threatening and difficult to treat. Despite antibiotics and supportive therapy, and in some cases advanced extensive surgery, mortality associated with invasive group A streptococcal postpartum endometritis, necrotizing fasciitis, and toxic shock syndrome remains high, up to 40% of postpartum septic deaths. It now accounts for more than 75,000 deaths worldwide every year. Postpartum women have a 20-fold increased incidence of GAS disease compared to non-pregnant women. Despite the high incidence, many invasive GAS infections are not diagnosed in a timely manner, resulting in potentially preventable maternal and neonatal deaths. In this paper the specific characteristics of GAS infection in the field of Ob/Gyn are brought to our attention, resulting in guidelines to improve our awareness, early recognition and timely treatment of the disease. New European prevalence data of vaginal GAS colonization are presented, alongside two original case histories. Additionally, aerobic vaginitis is proposed as a supplementary risk factor for invasive GAS diseases.

Published
2021

49. Enhancement of a Constitutive Friction Law by Considering Plastic Smoothing of Rough Surfaces in Dependency on the Bulk Stresses

Author

Florian Beyer, Löffler, M., Willner, K., Engel, U., and Merklein, M.

Subjects
Elasto-plastic contact, Friction, Constitutive friction law, Sheet-bulk metal forming, lcsh:Mechanical engineering and machinery, lcsh:TJ1-1570, Half-space model, Bulk stresses, Strain hardening
Abstract

The aim of this work is the improvement of a constitutive friction law which has been identified for the specific demands of sheet-bulk metal-forming. The constitutive friction law determines the friction shear stress in dependency on the plastic smoothing of the surface roughness, which also affects the real contact area. An experimental setup is introduced to determine the change of the surface roughness, which is influenced by the tensile bulk stress in the workpiece. In order to model the experimental results, a well-established half-space model is presented and improved to consider strain hardening. The half-space model is used to determine the change of the contact area as a function of the tensile bulk stress. This interdependency is also reflected by the proposed constitutive friction law.

Published
2016

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