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3. Folding and unfolding of a triple-branch DNA molecule with four conformational states

Author

Engel, Sandra, Alemany, Anna, Forns, Nuria, Maass, Philipp, and Ritort, Felix

Subjects
Physics - Biological Physics, Condensed Matter - Statistical Mechanics
Abstract

Single-molecule experiments provide new insights into biological processes hitherto not accessible by measurements performed on bulk systems. We report on a study of the kinetics of a triple-branch DNA molecule with four conformational states by pulling experiments with optical tweezers and theoretical modelling. Three distinct force rips associated with different transitions between the conformational states are observed in the folding and unfolding trajectories. By applying transition rate theory to a free energy model of the molecule, probability distributions for the first rupture forces of the different transitions are calculated. Good agreement of the theoretical predictions with the experimental findings is achieved. Furthermore, due to our specific design of the molecule, we found a useful method to identify permanently frayed molecules by estimating the number of opened basepairs from the measured force jump values., Comment: 17 pages, 12 figures

Published
2011
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5. Quercetin supplementation attenuates muscle wasting in cancer-associated cachexia in mice

Author

Levolger, Stef, Engel, Sandra, Ambagtsheer, Gisela, IJzermans, J.N.M., de Bruin, Ron, Levolger, Stef, Engel, Sandra, Ambagtsheer, Gisela, IJzermans, J.N.M., and de Bruin, Ron

Abstract

BACKGROUND: Quercetin is a flavonoid with reported antioxidant, anti-inflammatory and anti-aging effects, and may limit muscle wasting in cancer cachexia. OBJECTIVE: To investigate the effect of quercetin on muscle wasting in the murine C26 cancer-cachexia model and assess the feasibility of non-invasive micro-CT analysis of skeletal muscle. MATERIALS AND METHODS: Custom CRM(P) diets supplemented with 250 mg/kg quercetin (Q) were obtained. Thirty CD2F1 mice were equally randomized to non-tumor-bearing (NTB), C26 tumor-bearing (TB), TB + Q. All groups started their allocated diet and underwent hindlimb micro-CT. Bodyweight, food intake, and grip-strength were recorded periodically. After 21 days, repeat micro-CT was performed. Gastrocnemius (GCM) and tibialis anterior (TA) muscles were resected. mRNA expression of MuRF1, Atrogin-1, myogenin, and MyoD was determined. RESULTS: NTB and TB + Q gained 9.4% and 5.3% bodyweight respectively, TB lost 3.9%. Hind limb skeletal muscle volume remained stable for NTB and TB + Q, whereas TB decreased from 242.0 mm3 to 212.8 mm3. Mean GCM muscle weight was 175.2 mg (NTB), 171.3 mg (TB + Q) versus 125.5 mg (TB). A tendency towards decreased expression of atrogin-1 and MuRF1 was observed in TB + Q. CONCLUSION: Dietary quercetin supplementation limits bodyweight loss and muscle wasting in the C26-cancer-associated cachexia model.

Published
2021

8. Reply

Author

Verweij, Mariëlle, van Ginhoven, Tessa M., Mitchell, James R., Sluiter, Wim, van den Engel, Sandra, Roest, Henk P., Torabi, Elham, IJzermans, Jan N. M., Hoeijmakers, Jan H. J., and de Bruin, Ron W. F.

Published
2011
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19. Direct targeting of Gα q and Gα 11 oncoproteins in cancer cells

Author

Annala, Suvi, primary, Feng, Xiaodong, additional, Shridhar, Naveen, additional, Eryilmaz, Funda, additional, Patt, Julian, additional, Yang, JuHee, additional, Pfeil, Eva M., additional, Cervantes-Villagrana, Rodolfo Daniel, additional, Inoue, Asuka, additional, Häberlein, Felix, additional, Slodczyk, Tanja, additional, Reher, Raphael, additional, Kehraus, Stefan, additional, Monteleone, Stefania, additional, Schrage, Ramona, additional, Heycke, Nina, additional, Rick, Ulrike, additional, Engel, Sandra, additional, Pfeifer, Alexander, additional, Kolb, Peter, additional, König, Gabriele, additional, Bünemann, Moritz, additional, Tüting, Thomas, additional, Vázquez-Prado, José, additional, Gutkind, J. Silvio, additional, Gaffal, Evelyn, additional, and Kostenis, Evi, additional

Published
2019
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22. The Educational Oral History: An Approach to Teaching Basic English.

Author

Deem, James M. and Engel, Sandra A.

Abstract

Describes an introductory activity called educational oral history which was designed to improve communications between teachers and disadvantaged students in an integrated basic course in reading and writing. Concludes the project also raised students' pride and ability to comprehend and participate in the course. (SD)

Published
1988

23. Developing Literacy through Transcription.

Author

Deem, James M. and Engel, Sandra A.

Abstract

Describes a holistic strategy for teaching reading and writing skills, beginning with oral fluency, moving to simple transcription skills as part of an oral history exercise, and then to the production of a newsletter based on interviews. The strategy recognizes the relationship between the student's background and the English curriculum. (RS)

Published
1988

24. A signature of renal stress resistance induced by short-term dietary restriction, fasting, and protein restriction

Author

Jongbloed, Franny, Saat, Tanja, Verweij, Mariëlle, Payan Gomez, Cesar, Hoeijmakers, Jan, Engel, Sandra, van Oostrom, CT, Ambagtsheer, Gisela, Imholz, S, Pennings, JLA, van Steeg, H, IJzermans, J.N.M., Dolle, MET, de Bruin, Ron, Jongbloed, Franny, Saat, Tanja, Verweij, Mariëlle, Payan Gomez, Cesar, Hoeijmakers, Jan, Engel, Sandra, van Oostrom, CT, Ambagtsheer, Gisela, Imholz, S, Pennings, JLA, van Steeg, H, IJzermans, J.N.M., Dolle, MET, and de Bruin, Ron

Published
2017

26. Inactivated Mesenchymal Stem Cells Maintain Immunomodulatory Capacity

Author

Luk, Franka, primary, de Witte, Samantha F.H., additional, Korevaar, Sander S., additional, Roemeling-van Rhijn, Marieke, additional, Franquesa, Marcella, additional, Strini, Tanja, additional, van den Engel, Sandra, additional, Gargesha, Madhusudhana, additional, Roy, Debashish, additional, Dor, Frank J.M.F., additional, Horwitz, Edwin M., additional, de Bruin, Ron W.F., additional, Betjes, Michiel G.H., additional, Baan, Carla C., additional, and Hoogduijn, Martin J., additional

Published
2016
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30. Preoperative fasting protects against renal ischemia-reperfusion injury in aged and overweight mice

Author

Jongbloed, Franny, de Bruin, Ron W. F., Pennings, Jeroen L. A., Payán-Gómez, César, Engel, Sandra van den, Oostrom, Conny T. van, de Bruin, Alain, Hoeijmakers, Jan H. J., Steeg, Harry van, IJzermans, Jan N. M., Dollé, Martijn E. T., Jongbloed, Franny, de Bruin, Ron W. F., Pennings, Jeroen L. A., Payán-Gómez, César, Engel, Sandra van den, Oostrom, Conny T. van, de Bruin, Alain, Hoeijmakers, Jan H. J., Steeg, Harry van, IJzermans, Jan N. M., and Dollé, Martijn E. T.

Abstract

Ischemia-reperfusion injury (IRI) is inevitable during kidney transplantation leading to oxidative stress and inflammation. We previously reported that preoperative fasting in young-lean male mice protects against IRI. Since patients are generally of older age with morbidities possibly leading to a different response to fasting, we investigated the effects of preoperative fasting on renal IRI in aged-overweight male and female mice. Male and female F1-FVB/C57BL6-hybrid mice, average age 73 weeks weighing 47.2 grams, were randomized to preoperative ad libitum feeding or 3 days fasting, followed by renal IRI. Body weight, kidney function and survival of the animals were monitored until day 28 postoperatively. Kidney histopathology was scored for all animals and gene expression profiles after fasting were analyzed in kidneys of young and aged male mice. Preoperative fasting significantly improved survival after renal IRI in both sexes compared with normal fed mice. Fasted groups had a better kidney function shown by lower serum urea levels after renal IRI. Histopathology showed less acute tubular necrosis and more regeneration in kidneys from fasted mice. A mRNA analysis indicated the involvement of metabolic processes including fatty acid oxidation and retinol metabolism, and the NRF2-mediated stress response. Similar to young-lean, healthy male mice, preoperative fasting protects against renal IRI in aged-overweight mice of both genders. These findings suggest a general protective response of fasting against renal IRI regardless of age, gender, body weight and genetic background. Therefore, fasting could be a non-invasive intervention inducing increased oxidative stress resistance in older and overweight patients as well. © 2014 Jongbloed et al.

Published
2014

31. Preoperative fasting protects against renal ischemia-reperfusion injury in aged and overweight mice

Author

LS Pathobiologie, Tissue Repair, PB TR, Jongbloed, Franny, De Bruin, Ron W F, Pennings, Jeroen L A, Payán-Gómez, César, Van Den Engel, Sandra, Van Oostrom, Conny T., De Bruin, Alain, Hoeijmakers, Jan H J, Van Steeg, Harry, IJzermans, Jan N M, Dollé, Martijn E T, LS Pathobiologie, Tissue Repair, PB TR, Jongbloed, Franny, De Bruin, Ron W F, Pennings, Jeroen L A, Payán-Gómez, César, Van Den Engel, Sandra, Van Oostrom, Conny T., De Bruin, Alain, Hoeijmakers, Jan H J, Van Steeg, Harry, IJzermans, Jan N M, and Dollé, Martijn E T

Published
2014

33. Preoperative Fasting Protects against Renal Ischemia-Reperfusion Injury in Aged and Overweight Mice

Author

Jongbloed, Franny, primary, de Bruin, Ron W. F., additional, Pennings, Jeroen L. A., additional, Payán-Gómez, César, additional, van den Engel, Sandra, additional, van Oostrom, Conny T., additional, de Bruin, Alain, additional, Hoeijmakers, Jan H. J., additional, van Steeg, Harry, additional, IJzermans, Jan N. M., additional, and Dollé, Martijn E. T., additional

Published
2014
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46. Bulimia Nervosa in Bulimia Nervosa in DSM-5

Author

Wolfe, Barbara E., Hannon-Engel, Sandra L., and Mitchell, James E.

Abstract

CME Educational Objectives1.Outline the core clinical features of bulimia nervosa (BN).2.Describe the concerns surrounding the Describe the concerns surrounding the DSM-IVcriteria for BN.3.Understand the rationale for changes in the diagnostic criteria for BN as recommended for Understand the rationale for changes in the diagnostic criteria for BN as recommended for DSM-5.Bulimia nervosa, a disorder first introduced as “bulimia” in the Bulimia nervosa, a disorder first introduced as “bulimia” in the Diagnostic and Statistical Manual of Mental Disorders, Third Edition,1is characterized by recurrent binge eating episodes followed by inappropriate compensatory behaviors (such as, self-induced vomiting, laxative abuse, excessive exercising, and fasting) to prevent weight gain. The disorder is more common in females than in males, with onset typically in adolescence and young adulthood.2

Published
2012
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47. Direct targeting of Gαqand Gα11oncoproteins in cancer cells

Author

Annala, Suvi, Feng, Xiaodong, Shridhar, Naveen, Eryilmaz, Funda, Patt, Julian, Yang, JuHee, Pfeil, Eva M., Cervantes-Villagrana, Rodolfo Daniel, Inoue, Asuka, Häberlein, Felix, Slodczyk, Tanja, Reher, Raphael, Kehraus, Stefan, Monteleone, Stefania, Schrage, Ramona, Heycke, Nina, Rick, Ulrike, Engel, Sandra, Pfeifer, Alexander, Kolb, Peter, König, Gabriele, Bünemann, Moritz, Tüting, Thomas, Vázquez-Prado, José, Gutkind, J. Silvio, Gaffal, Evelyn, and Kostenis, Evi

Abstract

Inhibition of constitutively activated, oncogenic G proteins inhibits uveal melanoma tumor growth in mice.

Published
2019
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