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Made available in DSpace on 2019-10-04T12:39:57Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-07-04 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) The genera Eriosema and Rhynchosia, which comprise approximately 150 and 230 species, respectively, belong to the tribe Phaseoleae and to the subtribe Cajaninae. The pollen morphology of 49 species of Eriosema and 13 of Rhynchosia were studied using scanning electron microscopy to identify diagnostic characteristics that could aid in the taxonomic circumscription of those genera. The pollen grains analysed were obtained from specimens deposited in the BOTU, CEPEC, IPA, HRB, HUEFS, OUPR, P, S, UFC, and UFRJ herbaria. The species studied demonstrated that the pollen grains dispersed in monads, are isopolar or heteropolar, triporate or tricolporate, angulaperturate; with the endo-aperture membrane generally psilate and the exine largely reticulate heterobrocate, except for R. claussenii which is microreticulate. We discuss these diverse characteristics, including the ornamentation of the endo-aperture membrane and the margins of the apertures and reticula, to obtain information that possibly could be used to differentiate between the two genera. Although this proved not to be possible using pollen, because many characters overlap between the two genera, nevertheless some species display unique, taxonomically useful, characters, such as R. platyphylla which has an apocolpium in the shape of a 'Y', E. bauchiense with a verrucate exine ornamentation, and E. benthamianum with fossulate lumina. Additionally, two pollen types were identified in Eriosema, one group of species with 3-porate pollen grains and the other composed of species with 3-colporate grains. Univ Estado Bahia, Programa Posgrad Biodiversidade Vegetal PPGBVeg, Dept Educ, Campus 8, Paulo Afonso, Brazil Univ Estado Bahia, Dept Ciencias Humanas, Campus 6, Caetite, Brazil Univ Estadual Paulista, Inst Biociencias, Dept Bot, Programa Posgrad Ciencias Biol Bot, Botucatu, SP, Brazil Royal Bot Gardens, Comparat Plant & Fungal Biol Dept, London, England Univ Estadual Paulista, Inst Biociencias, Dept Bot, Programa Posgrad Ciencias Biol Bot, Botucatu, SP, Brazil

Made available in DSpace on 2018-12-11T17:21:12Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-10-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Gloxiniinae comprises herbs and subshrubs usually growing on rocky outcrops, rocky fields or gallery forests. For Gesneriaceae in some cases variations in pollen morphology allow the differentiation of genera and species. We studied the pollen grains of ten Brazilian species of Gloxiniinae (Gesneriaceae), including the genera Chautemsia, Gloxinia, Goyazia, Mandirola and Seemannia with the purposes of describing the pollen morphology of these genera, and contributing to the taxonomic characterization of the group and a better delimitation of genera and species of Gesneriaceae. The pollen grains were acetolyzed, measured, photographed and described qualitatively. Quantitative data were analyzed through descriptive and multivariate statistics. Non-acetolyzed pollen grains were observed in SEM and TEM in order to provide more details of the ornamentation and the structure of the exine. The analyzed species differ as to the pollen amb, shape, length, width and ends of colpi, presence or absence of margo, type of endoaperture and exine ornamentation. We found circular, subcircular and subtriangular amb, oblate spheroidal and subprolate shape, long or very long colpi, narrow, wide or very wide, with or without margo, lalongate or lolongate endoaperture in microreticulate or reticulate pollen grains. The reticulate ornamentation appears in the Gloxinia species’ pollen grains, and lolongate endoapertures represent the pattern for the subtribe pollen grains except in Chautemsia. The pollen grain metrics examined by principal component analysis allow generic distinction. We conclude that qualitative and quantitative pollen data help to delimit the genera and species of Gloxiniinae. Departamento de Biologia Aplicada à Agropecuária Faculdade de Ciências Agrárias e Veterinárias (FCAV) UNESP—Univ. Estadual Paulista, Via de Acesso Prof. Paulo Donato Castellane, s/n Centro de Ciências Naturais e Humanas UFABC – Univ. Federal do ABC, Rua São Paulo, s/nº - Jardim Antares Laboratoire de Systématique Végétale et Biodiversité Conservatoire et Jardin botaniques de la Ville de Genève & Université de Genève, CP 71 Instituto de Botânica Núcleo de Pesquisas em Palinologia, Av. Miguel Stéfano, 3687 Água Funda Departamento de Biologia Aplicada à Agropecuária Faculdade de Ciências Agrárias e Veterinárias (FCAV) UNESP—Univ. Estadual Paulista, Via de Acesso Prof. Paulo Donato Castellane, s/n FAPESP: 2011/20269-9 FAPESP: 2015/02707-0

The pollen grain morphology of Jacaratia corumbensis Kuntze, J. spinosa (Aubl.) A.DC., Vasconcellea quercifolia A. St.-Hil., and Carica papaya L. from Argentine samples were examined for the first time, using light and scanning electron microscopes. Observations and measurements were performed on acetolyzed pollen grains. Principal-components analysis was performed for quantitative morphological variables. Pollen grains were tri-colporate with lalongate endoaperture, oblate spheroidal to prolate, medium, exine bireticulate or reticulate, with a granulate lumina. A fastigium was present. Novel palynological data in this study for species identification revealed the presence of margo in C. papaya and variations in endoaperture shapes in all the species. The endoaperture ends often were H-shaped or horn-shaped. A biplot showed clear separation of the four taxa based on the palynological traits. Vasconcellea quercifolia was strongly correlated with equatorial diameter, equatorial diameter in polar view, exine thickness, and polar diameter, whereas J. corumbensis and J. spinosa were positively correlated with polar index and polar area index, respectively. The distance between two colpi allowed the discrimination of C. papaya. We confirmed the homogenous nature for most of the qualitative palynological characters in Caricaceae, and demonstrated that pollen morphology in combination with statistical analyses is a reliable tool for delimiting taxonomic groups. Fil: Zini, Lucia Melisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina Fil: Carrera, Constanza Soledad. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina Fil: Lattar, Elsa Clorinda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina Fil: Ferrucci, María Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina

Pollen and orbicule morphology of 26 species in the tribe Hillieae is described based on observations by light, scanning and transmission electron microscopy. Pollen and orbicule characters are critically evaluated and discussed in the context of existing hypotheses of systematic relationships within the tribe. Pollen is 3-zonocolporate with a perforate, microreticulate, reticulate or eutectate sexine. In the two species of Blepharidium, however, the pollen has one, four or five apertures. These pollen morphological data were incorporated into an existing macromorphological matrix of the group and cladistically analysed. The resulting phylogenies indicate that it may be appropriate to reduce Cosmibuena to a subgenus of the genus Hillia, while Blepharidium should be removed from Hillieae because of the deviating pollen type that is unique to the Rubiaceae. All species investigated produce orbicules, which are mostly spherical and possess an electron-lucent core that is sometimes characteristically flattened. (C) 2004 The Linnean Society of London, Botanical Journal of the Linnean Society, 2004, 146, 303-321. ispartof: Botanical journal of the Linnean Society vol:146 issue:3 pages:303-321 status: published