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2 results on '"Endo-H"'

1. Chemical Secretory Pathway Modulation in Plant Protoplasts

Author

Michele Bellucci, Francesca De Marchis, and Andrea Pompa

Subjects
0106 biological sciences, 0301 basic medicine, medicine.diagnostic_test, Immunoprecipitation, fungi, food and beverages, Tunicamycin, biochemical phenomena, metabolism, and nutrition, Protoplast, Golgi apparatus, Brefeldin A, Immunofluorescence, 01 natural sciences, Transport protein, Cell biology, 03 medical and health sciences, chemistry.chemical_compound, symbols.namesake, 030104 developmental biology, chemistry, medicine, symbols, BFA, Endo-H, Golgi-mediated protein traffic, Pulse-chase, Tobacco, Secretory pathway, 010606 plant biology & botany
Abstract

The classical Golgi pathway is not the only mechanism for vacuolar protein transport in plants because alternative transport mechanisms have been described. The existence of these alternative pathways can be demonstrated using several chemicals and here we describe the use of brefeldin A (BFA), endo-β-N-acetylglucosaminidase H (Endo-H), and tunicamycin, on isolated tobacco leaf protoplasts. Two main methods are illustrated in this chapter, protoplast pulse-chase followed by protein immunoprecipitation, and protoplast immunofluorescence.

Published
2016

2. Chemical Secretory Pathway Modulation in Plant Protoplasts.

Author

De Marchis F, Pompa A, and Bellucci M

Subjects
Biological Transport, Glycoside Hydrolases metabolism, Golgi Apparatus metabolism, Immunoprecipitation, Plant Leaves metabolism, Polysaccharides metabolism, Protein Transport, Nicotiana metabolism, Vacuoles metabolism, Plants metabolism, Protoplasts metabolism, Secretory Pathway
Abstract

The classical Golgi pathway is not the only mechanism for vacuolar protein transport in plants because alternative transport mechanisms have been described. The existence of these alternative pathways can be demonstrated using several chemicals and here we describe the use of brefeldin A (BFA), endo-β-N-acetylglucosaminidase H (Endo-H), and tunicamycin, on isolated tobacco leaf protoplasts. Two main methods are illustrated in this chapter, protoplast pulse-chase followed by protein immunoprecipitation, and protoplast immunofluorescence.

Published
2016
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