Search

Your search keyword '"Empel, Joanna"' showing total 145 results
Start Over Author "Empel, Joanna"
145 results on '"Empel, Joanna"'

2. Improving Activity of New Arylurea Agents against Multidrug-Resistant and Biofilm-Producing Staphylococcus epidermidis

Author

Canale, Vittorio, primary, Skiba-Kurek, Iwona, additional, Klesiewicz, Karolina, additional, Papież, Monika, additional, Ropek, Marlena, additional, Pomierny, Bartosz, additional, Piska, Kamil, additional, Koczurkiewicz-Adamczyk, Paulina, additional, Empel, Joanna, additional, Karczewska, Elżbieta, additional, and Zajdel, Paweł, additional

Published
2024
Full Text
View/download PDF

3. Foodborne Origin and Local and Global Spread of Staphylococcus saprophyticus Causing Human Urinary Tract Infections

Author

Lawal, Opeyemi U., Fraqueza, Maria J., Bouchami, Ons, Worning, Peder, Bartels, Mette D., Gonsalves, Maria L., Paixao, Paulo, Gonsalves, Elsa, Toscano, Cristina, Empel, Joanna, Urbas, Matgorzata, Dominguez, M. Angeles, Westh, Henrik, de Lencastre, Hermmia, and Miragaia, Maria

Subjects
Urinary tract infections -- Causes of, Staphylococcus -- Distribution -- Health aspects -- Genetic aspects, Company distribution practices, Health
Abstract

Staphylococcus saprophyticus is the cause of uncomplicated urinary tract infection (UTI) in 10%-20% of young women (1). Despite a greater successful treatment rate, S. saprophyticus UTI has a higher recurrent [...]

Published
2021
Full Text
View/download PDF

5. Design and synthesis of novel arylurea derivatives of aryloxy(1-phenylpropyl) alicyclic diamines with potential biological activity against multidrug-resistant Gram-positive bacteria

Author

Canale, Vittorio, primary, Czekajewska, Joanna, additional, Klesiewicz, Karolina, additional, Papież, Monika, additional, Kuziak, Agata, additional, Witek, Karolina, additional, Piska, Kamil, additional, Niemiec, Dagmara, additional, Kasza, Patryk, additional, Pękala, Elżbieta, additional, Empel, Joanna, additional, Tomczak, Magdalena, additional, Karczewska, Elżbieta, additional, and Zajdel, Paweł, additional

Published
2023
Full Text
View/download PDF

6. Interventions to reduce colonisation and transmission of antimicrobial-resistant bacteria in intensive care units: an interrupted time series study and cluster randomised trial

Author

Derde, Lennie P G, Cooper, Ben S, Goossens, Herman, Malhotra-Kumar, Surbhi, Willems, Rob J L, Gniadkowski, Marek, Hryniewicz, Waleria, Empel, Joanna, Dautzenberg, Mirjam J D, Annane, Djillali, Aragão, Irene, Chalfine, Annie, Dumpis, Uga, Esteves, Francisco, Giamarellou, Helen, Muzlovic, Igor, Nardi, Giuseppe, Petrikkos, George L, Tomic, Viktorija, Martí, Antonio Torres, Stammet, Pascal, Brun-Buisson, Christian, and Bonten, Marc J M

Published
2014
Full Text
View/download PDF

7. MRSA surveillance programmes worldwide: moving towards a harmonised international approach

Author

Baede, Valerie O., David, Michael Z., Andrasevic, Arjana Tambic, Blanc, Dominique S., Borg, Michael, Brennan, Grainne, Catry, Boudewijn, Chabaud, Aurelie, Empel, Joanna, Enger, Hege, Hallin, Marie, Ivanova, Marina, Kronenberg, Andreas, Kuntaman, Kuntaman, Larsen, Anders Rhod, Latour, Katrien, Lindsay, Jodi A., Pichon, Bruno, Santosaningsih, Dewi, Schouls, Leo M., Vandenesch, Francois, Werner, Guido, Zabicka, Dorota, Zemlickova, Helena, Seifert, Harald, Vos, Margreet C., Baede, Valerie O., David, Michael Z., Andrasevic, Arjana Tambic, Blanc, Dominique S., Borg, Michael, Brennan, Grainne, Catry, Boudewijn, Chabaud, Aurelie, Empel, Joanna, Enger, Hege, Hallin, Marie, Ivanova, Marina, Kronenberg, Andreas, Kuntaman, Kuntaman, Larsen, Anders Rhod, Latour, Katrien, Lindsay, Jodi A., Pichon, Bruno, Santosaningsih, Dewi, Schouls, Leo M., Vandenesch, Francois, Werner, Guido, Zabicka, Dorota, Zemlickova, Helena, Seifert, Harald, and Vos, Margreet C.

Abstract

A B S T R A C T Multinational surveillance programmes for methicillin-resistant Staphylococcus aureus (MRSA) are dependent on national structures for data collection. This study aimed to capture the diversity of national MRSA surveillance programmes and to propose a framework for harmonisation of MRSA surveillance. The International Society of Antimicrobial Chemotherapy (ISAC) MRSA Working Group conducted a structured survey on MRSA surveillance programmes and organised a webinar to discuss the programmes' strengths and challenges as well as guidelines for harmonisation. Completed surveys represented 24 MRSA surveil-lance programmes in 16 countries. Several countries reported separate epidemiological and microbiolog-ical surveillance. Informing clinicians and national policy-makers were the most common purposes of surveillance. Surveillance of bloodstream infections (BSIs) was present in all programmes. Other invasive infections were often included. Three countries reported active surveillance of MRSA carriage. Method-ology and reporting of antimicrobial susceptibility, virulence factors, molecular genotyping and epidemi-ological metadata varied greatly. Current MRSA surveillance programmes rely upon heterogeneous data collection systems, which hampers international epidemiological monitoring and research. To harmonise MRSA surveillance, we suggest improving the integration of microbiological and epidemiological data, im-plementation of central biobanks for MRSA isolate collection, and inclusion of a representative sample of skin and soft-tissue infection cases in addition to all BSI cases.Crown Copyright (c) 2022 Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license ( http://creativecommons.org/licenses/by-nc-nd/4.0/ )

Published
2022

8. MRSA surveillance programmes worldwide: moving towards a harmonised international approach

Author

Baede, Valérie V.O., David, Michael M.Z., Andrasevic, Arjana Tambic, Blanc, Dominique, Borg, Michael, Brennan, Gráinne, Catry, Boudewijn, Chabaud, Aurélie, Empel, Joanna, Enger, Hege, Hallin, Marie, Ivanova, Marina, Kronenberg, Andreas, Kuntaman, Kuntaman, Larsen, Anders A.R., Latour, Katrien, Lindsay, Jodi A, Pichon, Bruno Marcel, Santosaningsih, Dewi, Schouls, Leo L.M., Vandenesch, François, Werner, Guido, Żabicka, Dorota, Zemlickova, Helena, Seifert, Harald, Vos, Margreet M.C., Baede, Valérie V.O., David, Michael M.Z., Andrasevic, Arjana Tambic, Blanc, Dominique, Borg, Michael, Brennan, Gráinne, Catry, Boudewijn, Chabaud, Aurélie, Empel, Joanna, Enger, Hege, Hallin, Marie, Ivanova, Marina, Kronenberg, Andreas, Kuntaman, Kuntaman, Larsen, Anders A.R., Latour, Katrien, Lindsay, Jodi A, Pichon, Bruno Marcel, Santosaningsih, Dewi, Schouls, Leo L.M., Vandenesch, François, Werner, Guido, Żabicka, Dorota, Zemlickova, Helena, Seifert, Harald, and Vos, Margreet M.C.

Abstract

Multinational surveillance programmes for methicillin-resistant Staphylococcus aureus (MRSA) are dependent on national structures for data collection. This study aimed to capture the diversity of national MRSA surveillance programmes and to propose a framework for harmonisation of MRSA surveillance. The International Society of Antimicrobial Chemotherapy (ISAC) MRSA Working Group conducted a structured survey on MRSA surveillance programmes and organised a webinar to discuss the programmes’ strengths and challenges as well as guidelines for harmonisation. Completed surveys represented 24 MRSA surveillance programmes in 16 countries. Several countries reported separate epidemiological and microbiological surveillance. Informing clinicians and national policy-makers were the most common purposes of surveillance. Surveillance of bloodstream infections (BSIs) was present in all programmes. Other invasive infections were often included. Three countries reported active surveillance of MRSA carriage. Methodology and reporting of antimicrobial susceptibility, virulence factors, molecular genotyping and epidemiological metadata varied greatly. Current MRSA surveillance programmes rely upon heterogeneous data collection systems, which hampers international epidemiological monitoring and research. To harmonise MRSA surveillance, we suggest improving the integration of microbiological and epidemiological data, implementation of central biobanks for MRSA isolate collection, and inclusion of a representative sample of skin and soft-tissue infection cases in addition to all BSI cases., SCOPUS: ar.j, info:eu-repo/semantics/published

Published
2022

9. MRSA surveillance programmes worldwide: moving towards a harmonised international approach

Author

Baede, Valérie O., primary, David, Michael Z., additional, Andrasevic, Arjana Tambic, additional, Blanc, Dominique S., additional, Borg, Michael, additional, Brennan, Grainne, additional, Catry, Boudewijn, additional, Chabaud, Aurélie, additional, Empel, Joanna, additional, Enger, Hege, additional, Hallin, Marie, additional, Ivanova, Marina, additional, Kronenberg, Andreas, additional, Kuntaman, Kuntaman, additional, Larsen, Anders Rhod, additional, Latour, Katrien, additional, Lindsay, Jodi A., additional, Pichon, Bruno, additional, Santosaningsih, Dewi, additional, Schouls, Leo M., additional, Vandenesch, François, additional, Werner, Guido, additional, Żabicka, Dorota, additional, Žemličková, Helena, additional, Seifert, Harald, additional, and Vos, Margreet C., additional

Published
2022
Full Text
View/download PDF

11. Staphylococcus saprophyticus Causing Infections in Humans Is Associated with High Resistance to Heavy Metals

Author

Lawal, Opeyemi U., primary, Fraqueza, Maria J., additional, Worning, Peder, additional, Bouchami, Ons, additional, Bartels, Mette D., additional, Goncalves, Luisa, additional, Paixão, Paulo, additional, Goncalves, Elsa, additional, Toscano, Cristina, additional, Empel, Joanna, additional, Urbaś, Malgorzata, additional, Domínguez, Maria A., additional, Westh, Henrik, additional, de Lencastre, Hermínia, additional, and Miragaia, Maria, additional

Published
2021
Full Text
View/download PDF

12. Staphylococcus saprophyticus From Clinical and Environmental Origins Have Distinct Biofilm Composition

Author

Lawal, Opeyemi U., primary, Barata, Marta, additional, Fraqueza, Maria J., additional, Worning, Peder, additional, Bartels, Mette D., additional, Goncalves, Luisa, additional, Paixão, Paulo, additional, Goncalves, Elsa, additional, Toscano, Cristina, additional, Empel, Joanna, additional, Urbaś, Malgorzata, additional, Domiìnguez, Maria A., additional, Westh, Henrik, additional, de Lencastre, Hermínia, additional, and Miragaia, Maria, additional

Published
2021
Full Text
View/download PDF

13. Staphylococcus saprophyticus From Clinical and Environmental Origins Have Distinct Biofilm Composition

Author

Lawal, Opeyemi U., Barata, Marta, Fraqueza, Maria J., Worning, Peder, Bartels, Mette D., Goncalves, Luisa, Paixão, Paulo, Goncalves, Elsa, Toscano, Cristina, Empel, Joanna, Urbaś, Malgorzata, Domiìnguez, Maria A., Westh, Henrik, de Lencastre, Hermínia, Miragaia, Maria, Lawal, Opeyemi U., Barata, Marta, Fraqueza, Maria J., Worning, Peder, Bartels, Mette D., Goncalves, Luisa, Paixão, Paulo, Goncalves, Elsa, Toscano, Cristina, Empel, Joanna, Urbaś, Malgorzata, Domiìnguez, Maria A., Westh, Henrik, de Lencastre, Hermínia, and Miragaia, Maria

Abstract

Biofilm formation has been shown to be critical to the success of uropathogens. Although Staphylococcus saprophyticus is a common cause of urinary tract infections, its biofilm production capacity, composition, genetic basis, and origin are poorly understood. We investigated biofilm formation in a large and diverse collection of S. saprophyticus (n = 422). Biofilm matrix composition was assessed in representative strains (n = 63) belonging to two main S. saprophyticus lineages (G and S) recovered from human infection, colonization, and food-related environment using biofilm detachment approach. To identify factors that could be associated with biofilm formation and structure variation, we used a pangenome-wide association study approach. Almost all the isolates (91%; n = 384/422) produced biofilm. Among the 63 representative strains, we identified eight biofilm matrix phenotypes, but the most common were composed of protein or protein–extracellular DNA (eDNA)–polysaccharides (38%, 24/63 each). Biofilms containing protein–eDNA–polysaccharides were linked to lineage G and environmental isolates, whereas protein-based biofilms were produced by lineage S and infection isolates (p < 0.05). Putative biofilm-associated genes, namely, aas, atl, ebpS, uafA, sasF, sasD, sdrH, splE, sdrE, sdrC, sraP, and ica genes, were found with different frequencies (3–100%), but there was no correlation between their presence and biofilm production or matrix types. Notably, icaC_1 was ubiquitous in the collection, while icaR was lineage G-associated, and only four strains carried a complete ica gene cluster (icaADBCR) except one that was without icaR. We provided evidence, using a comparative genomic approach, that the complete icaADBCR cluster was acquired multiple times by S. saprophyticus and originated from other coagulase-negative staphylococci. Overall, the composition of S. saprophyticus biofilms was distinct in environmental and clinical isolates, suggesting that modulation o

Published
2021

14. Staphylococcus saprophyticus causing infections in humans is associated with high resistance to heavy metals

Author

Lawal, Opeyemi U., Fraqueza, Maria J., Worning, Peder, Bouchami, Ons, Bartels, Mette D., Goncalves, Luisa, Paixão, Paulo, Goncalves, Elsa, Toscano, Cristina, Empel, Joanna, Urbaś, Malgorzata, Domínguez, Maria A., Westh, Henrik, de Lencastre, Hermínia, Miragaia, Maria, Lawal, Opeyemi U., Fraqueza, Maria J., Worning, Peder, Bouchami, Ons, Bartels, Mette D., Goncalves, Luisa, Paixão, Paulo, Goncalves, Elsa, Toscano, Cristina, Empel, Joanna, Urbaś, Malgorzata, Domínguez, Maria A., Westh, Henrik, de Lencastre, Hermínia, and Miragaia, Maria

Abstract

Staphylococcus saprophyticus is a common pathogen of the urinary tract, a heavy metal-rich environment, but information regarding its heavy metal resistance is unknown. We investigated 422 S. saprophyticus isolates from human infection and colonization/contamination, animals, and environmental sources for resistance to copper, zinc, arsenic, and cadmium using the agar dilution method. To identify the genes associated with metal resistance and assess possible links to pathogenicity, we accessed the whole-genome sequence of all isolates and used in silico and pangenome-wide association approaches. The MIC values for copper and zinc were uniformly high (1,600 mg/liter). Genes encoding copper efflux pumps (copA, copB, copZ, mco, and csoR) and zinc transporters (zinT, czrAB, znuBC, and zur) were abundant in the population (20 to 100%). Arsenic and cadmium showed various susceptibility levels. Genes encoding the ars operon (arsRDABC), an ABC transporter and a two-component permease, were linked to resistance to arsenic (MICs $ 1,600 mg/liter; 14% [58/422]; P, 0.05). At least three cad genes (cadA or cadC and cadD-cadX or czrC) and genes encoding multidrug efflux pumps and hyperosmoregulation in acidified conditions were associated with resistance to cadmium (MICs $ 200 mg/liter; 20% [85/422]; P, 0.05). These resistance genes were frequently carried by mobile genetic elements. Resistance to arsenic and cadmium were linked to human infection and a clonal lineage originating in animals (P, 0.05). Altogether, S. saprophyticus was highly resistant to heavy metals and accumulated multiple metal resistance determinants. The highest arsenic and cadmium resistance levels were associated with infection, suggesting resistance to these metals is relevant for S. saprophyticus pathogenicity.

Published
2021

16. A Kayvirus Distant Homolog of Staphylococcal Virulence Determinants and VISA Biomarker is a Phage Lytic Enzyme That Decreases S. aureus Tolerance to Vancomycin

Author

Głowacka-Rutkowska, Aleksandra, Ulatowska, Magdalena, Empel, Joanna, Kowalczyk, Magdalena, Boreczek, Jakub, and Łobocka, Małgorzata

Subjects
biology_other
Abstract

Staphylococcal bacteriophages of Kayvirus genus are candidates for therapeutic applications. One of their proteins, Tgl, is slightly similar to staphylococcal virulence factors, secreted autolysins of lytic transglycosylase motifs, IsaA and SceD. We show that Tgl is also a lytic enzyme secreted by bacterial transport system and localizes to cell peripheries, like IsaA and SceD. It caused lysis of E. coli cells expressing the cloned tgl gene, but could be overproduced when depleted of signal peptide. S. aureus cells producing Tgl lysed in the presence of nisin, which mimics the action of phage holin. In vitro, Tgl protein was able to destruct S. aureus cell walls. The production of Tgl decreased S. aureus tolerance to vancomycin, unlike the production of SceD, which is associated with the decreased sensitivity to vancomycin. In the genomes of kayviruses, the tgl gene is located a few genes away from gene lysK, encoding the major endolysin. While lysK is a late phage gene, tgl can be transcribed by a host RNA polymerase, as are phage early genes. Taken together our data indicate that tgl is a part of kayviruses lytic module and encodes an additional endolysin which can act in concert with LysK in cell lysis.

Published
2019

17. Emergence and spread of worldwide Staphylococcus aureus clones among cystic fibrosis patients

Author

Garbacz,Katarzyna, Piechowicz,Lidia, Podkowik,Magdalena, Mroczkowska,Aneta, Empel,Joanna, and Bania,Jacek

Subjects
cystic fibrosis, Staphylococcus aureus, Infection and Drug Resistance, Panton–Valentine leukocidin, MRSA, ST398 MRSA, Original Research, spa typing
Abstract

Katarzyna Garbacz,1 Lidia Piechowicz,2 Magdalena Podkowik,3 Aneta Mroczkowska,4 Joanna Empel,4 Jacek Bania3 1Department of Oral Microbiology, Medical University of Gdansk, Gdansk, Poland; 2Department of Medical Microbiology, Medical University of Gdansk, Gdansk, Poland; 3Department of Food Hygiene and Consumer Health Protection, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland; 4Department of Epidemiology and Clinical Microbiology, National Medicines Institute, Warsaw, Poland Background: The aim of this study was to assess the relatedness of molecular types of Staphylococcus aureus isolates colonizing cystic fibrosis (CF) patients with their antimicrobial resistance and prevalence of toxin genes. Methods: A total of 215 isolates from the airways of 107 patients with CF were tested for spa and SCCmec type, antimicrobial resistance and carriage of toxin genes. Results: t015, t084, t091, t700 and t002 were the largest group (approximately 25%) among all 69 identified spa types. Five new spa types, t14286, t14287, t14288, t14289 and t14290, were identified and registered. Isolates from CF patients were clustered into 11 multi-locus sequence typing clonal complexes, with CC30, CC22, CC97, CC45, CC15 and CC5 being the most frequent ones. Twelve (5.6%) methicillin-resistant S. aureus (MRSA) isolates and 102 (47.7%) multidrug-resistant isolates were identified, along with three SCCmec types (I, III and V). All isolates (both MRSA and methicillin-sensitive S. aureus) were Panton–Valentine leucocidin-negative, and 56.7% harbored egc genes. This was the first study documenting the presence of ST398-V-t571 livestock-associated MRSA in a European patient with CF. Conclusion: These findings imply that individuals with CF can also be colonized with animal-related ST398 MRSA, and justify constant monitoring of staphylococcal colonization and identification of epidemic S. aureus clones in this group. Keywords: Staphylococcus aureus, cystic fibrosis, ST398 MRSA, Panton–Valentine leukocidin, spa typing, MRSA

Published
2018

22. The Ability of Lytic Staphylococcal Podovirus vB_SauP_phiAGO1.3 to Coexist in Equilibrium With Its Host Facilitates the Selection of Host Mutants of Attenuated Virulence but Does Not Preclude the Phage Antistaphylococcal Activity in a Nematode Infection Model

Author

Głowacka-Rutkowska, Aleksandra, primary, Gozdek, Agnieszka, additional, Empel, Joanna, additional, Gawor, Jan, additional, Żuchniewicz, Karolina, additional, Kozińska, Aleksandra, additional, Dębski, Janusz, additional, Gromadka, Robert, additional, and Łobocka, Małgorzata, additional

Published
2019
Full Text
View/download PDF

23. European external quality assessments for identification, molecular typing and characterization of Staphylococcus aureus

Author

Deplano, Ariane, Dodémont, Magali, Denis, Olivier, Westh, Henrik, Gumpert, Heidi, Larsen, Anders Rhod, Larsen, Jesper, Kearns, Angela, Pichon, Bruno, Layer, Franziska, Schulte, Berit, Wolz, Christiane, Spiliopoulou, Iris, Brennan, Gráinne, Empel, Joanna, Hryniewicz, Waleria, de Lencastre, Herminia, Faria, Nuno Alexandre, Codita, Irina, Sabat, Artur J., Friedrich, Alexander W., Deurenberg, Ruud H., Tristan, Anne, Laurent, Frédéric, Vandenesch, François, Deplano, Ariane, Dodémont, Magali, Denis, Olivier, Westh, Henrik, Gumpert, Heidi, Larsen, Anders Rhod, Larsen, Jesper, Kearns, Angela, Pichon, Bruno, Layer, Franziska, Schulte, Berit, Wolz, Christiane, Spiliopoulou, Iris, Brennan, Gráinne, Empel, Joanna, Hryniewicz, Waleria, de Lencastre, Herminia, Faria, Nuno Alexandre, Codita, Irina, Sabat, Artur J., Friedrich, Alexander W., Deurenberg, Ruud H., Tristan, Anne, Laurent, Frédéric, and Vandenesch, François

Abstract

Objectives: We present the results of two European external quality assessments (EQAs) conducted in 2014 and 2016 under the auspices of the Study Group on Staphylococci and Staphylococcal Infections of ESCMID. The objective was to assess the performance of participating centres in characterizing Staphylococcus aureus using their standard in-house phenotypic and genotypic protocols. Methods: A total of 11 well-characterized blindly coded S. aureus (n " 9), Staphylococcus argenteus (n " 1) and Staphylococcus capitis (n " 1) strains were distributed to participants for analysis. Species identification, MIC determination, antimicrobial susceptibility testing, antimicrobial resistance and toxin gene detection and molecular typing including spa typing, SCCmec typing and MLST were performed. Results: Thirteen laboratories from 12 European countries participated in one EQA or both EQAs. Despite considerable diversity in the methods employed, good concordance (90%–100%) with expected results was obtained. Discrepancies were observed for: (i) identification of the S. argenteus strain; (ii) phenotypic detection of low-level resistance to oxacillin in the mecC-positive strain; (iii) phenotypic detection of the inducible MLSB strain; and (iv) WGS-based detection of some resistance and toxin genes. Conclusions: Overall, good concordance (90%–100%) with expected results was observed. In some instances, the accurate detection of resistance and toxin genes from WGS data proved problematic, highlighting the need for validated and internationally agreed-on bioinformatics pipelines before such techniques are implemented routinely by microbiology laboratories. We strongly recommend all national reference laboratories and laboratories acting as referral centres to participate in such EQA initiatives.

Published
2018

24. European external quality assessments for identification, molecular typing and characterization of Staphylococcus aureus

Author

Deplano, Ariane, primary, Dodémont, Magali, additional, Denis, Olivier, additional, Westh, Henrik, additional, Gumpert, Heidi, additional, Larsen, Anders Rhod, additional, Larsen, Jesper, additional, Kearns, Angela, additional, Pichon, Bruno, additional, Layer, Franziska, additional, Schulte, Berit, additional, Wolz, Christiane, additional, Spiliopoulou, Iris, additional, Brennan, Gráinne, additional, Empel, Joanna, additional, Hryniewicz, Waleria, additional, de Lencastre, Herminia, additional, Faria, Nuno Alexandre, additional, Codita, Irina, additional, Sabat, Artur J, additional, Friedrich, Alexander W, additional, Deurenberg, Ruud H, additional, Tristan, Anne, additional, Laurent, Frédéric, additional, and Vandenesch, François, additional

Published
2018
Full Text
View/download PDF

26. Livestock-associated Staphylococcus aureus on Polish pig farms

Author

Mroczkowska, Aneta, primary, Żmudzki, Jacek, additional, Marszałek, Natalia, additional, Orczykowska-Kotyna, Monika, additional, Komorowska, Iga, additional, Nowak, Agnieszka, additional, Grzesiak, Anna, additional, Czyżewska-Dors, Ewelina, additional, Dors, Arkadiusz, additional, Pejsak, Zygmunt, additional, Hryniewicz, Waleria, additional, Wyszomirski, Tomasz, additional, and Empel, Joanna, additional

Published
2017
Full Text
View/download PDF

27. KAEA (SUDPRO), a member of the ubiquitous KEOPS/EKC protein complex, regulates the arginine catabolic pathway and the expression of several other genes in Aspergillus nidulans

Author

Dzikowska, Agnieszka, primary, Grzelak, Anna, additional, Gawlik, Joanna, additional, Szewczyk, Edyta, additional, Mrozek, Paweł, additional, Borsuk, Piotr, additional, Koper, Michał, additional, Empel, Joanna, additional, Szczęsny, Paweł, additional, Piłsyk, Sebastian, additional, Pękala, Małgorzata, additional, and Weglenski, Piotr, additional

Published
2015
Full Text
View/download PDF

29. High genetic diversity among community-associated Staphylococcus aureus in Europe

Author

Rolo, Joana, Miragaia, Maria, Turlej-Rogacka, Agata, Empel, Joanna, Bouchami, Ons, Faria, Nuno Alexandre, Tavares , Ana, Hryniewicz, Waleria, Fluit, Adriaan C., de Lencastre, Hermínia, Nashev, Dimitar, Melter, Oto, Zemlickova, Helena, Fridrichová, Marta, Westh, Henrik T., Salmenlinna, Saara, Lina, Gerard, Spiliopoulou, Iris, Drougka, Eleanna, Kristóf, Katalin, Rozgonyi, Ferenc, Raponi, Giammarco, Ghezzi, Maria Cristina, Wulf, Mireille W.H., Codita, Irina, Ionescu, G., Nica, Maria, Lísková, Anna, Ruíz-Garbajosa, Patricia, Cantón, Rafael, Domínguez Luzón, M. A., Petersson, Ann Cathrine, Walker, Rebecca, Anderson, Rich, Andrews, Jennifer M., Instituto de Tecnologia Química e Biológica António Xavier (ITQB), and Molecular, Structural and Cellular Microbiology (MOSTMICRO)

Subjects
respiratory system, biochemical phenomena, metabolism, and nutrition, bacterial infections and mycoses
Abstract

BACKGROUND: Several studies have addressed the epidemiology of community-associated Staphylococcus aureus (CA-SA) in Europe; nonetheless, a comprehensive perspective remains unclear. In this study, we aimed to describe the population structure of CA-SA and to shed light on the origin of methicillin-resistant S. aureus (MRSA) in this continent. METHODS AND FINDINGS: A total of 568 colonization and infection isolates, comprising both MRSA and methicillin-susceptible S. aureus (MSSA), were recovered in 16 European countries, from community and community-onset infections. The genetic background of isolates was characterized by molecular typing techniques (spa typing, pulsed-field gel electrophoresis and multilocus sequence typing) and the presence of PVL and ACME was tested by PCR. MRSA were further characterized by SCCmec typing. We found that 59% of all isolates were associated with community-associated clones. Most MRSA were related with USA300 (ST8-IVa and variants) (40%), followed by the European clone (ST80-IVc and derivatives) (28%) and the Taiwan clone (ST59-IVa and related clonal types) (15%). A total of 83% of MRSA carried Panton-Valentine leukocidin (PVL) and 14% carried the arginine catabolic mobile element (ACME). Surprisingly, we found a high genetic diversity among MRSA clonal types (ST-SCCmec), Simpson's index of diversity = 0.852 (0.788-0.916). Specifically, about half of the isolates carried novel associations between genetic background and SCCmec. Analysis by BURP showed that some CA-MSSA and CA-MRSA isolates were highly related, suggesting a probable local acquisition/loss of SCCmec. CONCLUSIONS: Our results imply that CA-MRSA origin, epidemiology and population structure in Europe is very dissimilar from that of USA. publishersversion published

Published
2012

44. Population Structure and Oxacillin Resistance of Staphylococcus aureus from Pigs and Pork Meat in South-West of Poland.

Author

Krupa, Paweł, Bystroń, Jarosław, Podkowik, Magdalena, Empel, Joanna, Mroczkowska, Aneta, and Bania, Jacek

Subjects
MEAT microbiology, AGRICULTURE, ANIMAL experimentation, DRUG resistance in microorganisms, STAPHYLOCOCCUS aureus, SWINE, PHENOTYPES, OXACILLIN, METHICILLIN-resistant staphylococcus aureus, GENOTYPES
Abstract

The genotypes and oxacillin resistance of 420 S. aureus isolates from pigs (n=203) and pork (n=217) were analyzed. Among 18 spa types detected in S. aureus from pig t011, t021, t034, t091, t318, t337, and t1334 were the most frequent. Among 30 spa types found in S. aureus isolates from pork t084, t091, t499, t4309, t12954, and t13074 were dominant. The animal S. aureus isolates were clustered into MLST clonal complexes CC7, CC9, CC15, CC30, and CC398 and meat-derived isolates to CC1, CC7, and CC15. Thirty-six MRSA were isolated exclusively from pigs. All MRSA were classified to spa t011 SCCmecV. BORSA phenotype was found in 14% S. aureus isolates from pigs and 10% isolates from pork meat. spa t034 dominated among BORSA from pigs and t091 among meat-derived BORSA. This is the first report on spa types and oxacillin resistance of S. aureus strains from pigs and pork meat in Poland. Besides S. aureus CC9, CC30, and CC398 known to be distributed in pigs, the occurrence of genotype belonging to CC7 in this species has been reported for the first time. To our knowledge it is also the first report concerning CC398 BORSA isolates from pigs and pork meat. [ABSTRACT FROM AUTHOR]

Published
2015
Full Text
View/download PDF

46. Comparison of Multiple-Locus Variable-Number Tandem-Repeat Analysis with Pulsed-Field Gel Electrophoresis, spa Typing, and Multilocus Sequence Typing for Clonal Characterization of Staphylococcus aureus Isolates

Author

Malachowa, Natalia, primary, Sabat, Artur, additional, Gniadkowski, Marek, additional, Krzyszton-Russjan, Jolanta, additional, Empel, Joanna, additional, Miedzobrodzki, Jacek, additional, Kosowska-Shick, Klaudia, additional, Appelbaum, Peter C., additional, and Hryniewicz, Waleria, additional

Published
2005
Full Text
View/download PDF

50. High Genetic Diversity among Community-Associated Staphylococcus aureus in Europe: Results from a Multicenter Study.

Author

Rolo, Joana, Miragaia, Maria, Turlej-Rogacka, Agata, Empel, Joanna, Bouchami, Ons, Faria, Nuno A., Tavares, Ana, Hryniewicz, Waleria, Fluit, Ad C., and Lencastre, Hermínia de

Subjects
STAPHYLOCOCCUS aureus infections, INFECTIOUS disease transmission, EPIDEMIOLOGY, METHICILLIN-resistant staphylococcus aureus, POLYMERASE chain reaction
Abstract

Background: Several studies have addressed the epidemiology of community-associated Staphylococcus aureus (CA-SA) in Europe; nonetheless, a comprehensive perspective remains unclear. In this study, we aimed to describe the population structure of CA-SA and to shed light on the origin of methicillin-resistant S. aureus (MRSA) in this continent. Methods and Findings: A total of 568 colonization and infection isolates, comprising both MRSA and methicillin-susceptible S. aureus (MSSA), were recovered in 16 European countries, from community and community-onset infections. The genetic background of isolates was characterized by molecular typing techniques (spa typing, pulsed-field gel electrophoresis and multilocus sequence typing) and the presence of PVL and ACME was tested by PCR. MRSA were further characterized by SCCmec typing. We found that 59% of all isolates were associated with community-associated clones. Most MRSA were related with USA300 (ST8-IVa and variants) (40%), followed by the European clone (ST80-IVc and derivatives) (28%) and the Taiwan clone (ST59-IVa and related clonal types) (15%). A total of 83% of MRSA carried Panton-Valentine leukocidin (PVL) and 14% carried the arginine catabolic mobile element (ACME). Surprisingly, we found a high genetic diversity among MRSA clonal types (ST-SCCmec), Simpson's index of diversity = 0.852 (0.788-0.916). Specifically, about half of the isolates carried novel associations between genetic background and SCCmec. Analysis by BURP showed that some CA-MSSA and CA-MRSA isolates were highly related, suggesting a probable local acquisition/loss of SCCmec. Conclusions: Our results imply that CA-MRSA origin, epidemiology and population structure in Europe is very dissimilar from that of USA. [ABSTRACT FROM AUTHOR]

Published
2012
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results