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32 results on '"Emnett, Ryan J."'

1. Supplementary Data from Array-Based Comparative Genomic Hybridization Identifies CDK4 and FOXM1 Alterations as Independent Predictors of Survival in Malignant Peripheral Nerve Sheath Tumor

Author

Yu, Jinsheng, primary, Deshmukh, Hrishikesh, primary, Payton, Jacqueline E., primary, Dunham, Christopher, primary, Scheithauer, Bernd W., primary, Tihan, Tarik, primary, Prayson, Richard A., primary, Guha, Abhijit, primary, Bridge, Julia A., primary, Ferner, Rosalie E., primary, Lindberg, Guy M., primary, Gutmann, Rebecca J., primary, Emnett, Ryan J., primary, Salavaggione, Lorena, primary, Gutmann, David H., primary, Nagarajan, Rakesh, primary, Watson, Mark A., primary, and Perry, Arie, primary

Published
2023
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2. Data from Array-Based Comparative Genomic Hybridization Identifies CDK4 and FOXM1 Alterations as Independent Predictors of Survival in Malignant Peripheral Nerve Sheath Tumor

Author

Yu, Jinsheng, primary, Deshmukh, Hrishikesh, primary, Payton, Jacqueline E., primary, Dunham, Christopher, primary, Scheithauer, Bernd W., primary, Tihan, Tarik, primary, Prayson, Richard A., primary, Guha, Abhijit, primary, Bridge, Julia A., primary, Ferner, Rosalie E., primary, Lindberg, Guy M., primary, Gutmann, Rebecca J., primary, Emnett, Ryan J., primary, Salavaggione, Lorena, primary, Gutmann, David H., primary, Nagarajan, Rakesh, primary, Watson, Mark A., primary, and Perry, Arie, primary

Published
2023
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3. Supplementary Figure 1 from Distinct Genetic Signatures among Pilocytic Astrocytomas Relate to Their Brain Region Origin

Author

Sharma, Mukesh K., primary, Mansur, David B., primary, Reifenberger, Guido, primary, Perry, Arie, primary, Leonard, Jeffrey R., primary, Aldape, Kenneth D., primary, Albin, Meredith G., primary, Emnett, Ryan J., primary, Loeser, Simon, primary, Watson, Mark A., primary, Nagarajan, Rakesh, primary, and Gutmann, David H., primary

Published
2023
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4. Supplementary Figure 2 from Distinct Genetic Signatures among Pilocytic Astrocytomas Relate to Their Brain Region Origin

Author

Sharma, Mukesh K., primary, Mansur, David B., primary, Reifenberger, Guido, primary, Perry, Arie, primary, Leonard, Jeffrey R., primary, Aldape, Kenneth D., primary, Albin, Meredith G., primary, Emnett, Ryan J., primary, Loeser, Simon, primary, Watson, Mark A., primary, Nagarajan, Rakesh, primary, and Gutmann, David H., primary

Published
2023
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5. Supplementary Table 1 from Distinct Genetic Signatures among Pilocytic Astrocytomas Relate to Their Brain Region Origin

Author

Sharma, Mukesh K., primary, Mansur, David B., primary, Reifenberger, Guido, primary, Perry, Arie, primary, Leonard, Jeffrey R., primary, Aldape, Kenneth D., primary, Albin, Meredith G., primary, Emnett, Ryan J., primary, Loeser, Simon, primary, Watson, Mark A., primary, Nagarajan, Rakesh, primary, and Gutmann, David H., primary

Published
2023
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6. Supplementary Figure Legends 1-2 from Distinct Genetic Signatures among Pilocytic Astrocytomas Relate to Their Brain Region Origin

Author

Sharma, Mukesh K., primary, Mansur, David B., primary, Reifenberger, Guido, primary, Perry, Arie, primary, Leonard, Jeffrey R., primary, Aldape, Kenneth D., primary, Albin, Meredith G., primary, Emnett, Ryan J., primary, Loeser, Simon, primary, Watson, Mark A., primary, Nagarajan, Rakesh, primary, and Gutmann, David H., primary

Published
2023
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11. Ab initio molecular orbital and density functional studies on the solvolysis of sarin and O,S-dimethyl methylphosphonothiolate, a VX-like compound

Author

Seckutte, Jolita, Menke, Jessica L., Emnett, Ryan J., Patterson, Eric V., and Cramer, Chrisopher J.

Subjects
Density functionals -- Usage, Molecular orbitals -- Research, Hydrolysis -- Research, Nerve gas -- Chemical properties, Methyl groups -- Chemical properties, Biological sciences, Chemistry
Abstract

Potential energy surfaces for the alkaline hydrolysis of sarin and O,S-dimethyl methylphosphonothiolate, a VX model compound, and the perhydrolysis of the latter are computed at the MP2/6-31+G(d)//mPW1K/MIDI level of theory. Excellent agreement with the experimental enthalpy of activation for alkaline hydrolysis of sarin was found.

Published
2005

19. Conditional KIAA1549:BRAF mice reveal brain region- and cell type-specific effects

Author

Kaul, Aparna, Chen, Yi-Hsien, Emnett, Ryan J., Gianino, Scott M., and Gutmann, David H.

Subjects
Proto-Oncogene Proteins B-raf, endocrine system diseases, Oncogene Proteins, Fusion, Green Fluorescent Proteins, Brain, Mice, Transgenic, Astrocytoma, digestive system diseases, Article, Mice, nervous system, Neural Stem Cells, Organ Specificity, Astrocytes, Cerebellum, Models, Animal, Animals, Humans, Mitogen-Activated Protein Kinases, skin and connective tissue diseases, Child, neoplasms, Neuroglia, Cell Proliferation
Abstract

Low-grade brain tumors (pilocytic astrocytomas) that result from a genomic rearrangement in which the BRAF kinase domain is fused to the amino terminal of the KIAA1549 gene (KIAA1549:BRAF fusion; f-BRAF) commonly arise in the cerebellum of young children. To model this temporal and spatial specificity in mice, we generated conditional KIAA1549:BRAF strains that coexpresses green fluorescent protein (GFP). Although both primary astrocytes and neural stem cells (NSCs) from these mice express f-BRAF and GFP as well as exhibit increased MEK activity, only f-BRAF-expressing NSCs exhibit increased proliferation in vitro. Using Cre driver lines in which KIAA1549:BRAF expression was directed to NSCs (f-BRAF; BLBP-Cre mice), astrocytes (f-BRAF; GFAP-Cre mice), and NG2 progenitor cells (f-BRAF; NG2-Cre mice), increased glial cell numbers were observed only in the cerebellum of f-BRAF; BLBP-Cre mice in vivo. The availability of this unique KIAA1549:BRAF conditional transgenic mouse strain will enable future mechanistic studies aimed at defining the developmentally-regulated temporal and spatial determinants that underlie low-grade astrocytoma formation in children.

Published
2013

20. F11R Is a Novel Monocyte Prognostic Biomarker for Malignant Glioma

Author

Pong, Winnie W., primary, Walker, Jason, additional, Wylie, Todd, additional, Magrini, Vincent, additional, Luo, Jingqin, additional, Emnett, Ryan J., additional, Choi, Jaebok, additional, Cooper, Matthew L., additional, Griffith, Malachi, additional, Griffith, Obi L., additional, Rubin, Joshua B., additional, Fuller, Gregory N., additional, Piwnica-Worms, David, additional, Feng, Xi, additional, Hambardzumyan, Dolores, additional, DiPersio, John F., additional, Mardis, Elaine R., additional, and Gutmann, David H., additional

Published
2013
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23. Evaluation of Tissue Homogenization to Support the Generation of GMP-Compliant Mesenchymal Stromal Cells from the Umbilical Cord.

Author

Emnett, Ryan J., Kaul, Aparna, Babic, Aleksandar, Geiler, Vicki, Regan, Donna, Gross, Gilad, and Akel, Salem

Subjects
*MESENCHYMAL stem cells, *GUANOSINE monophosphate phosphodiesterase, *UMBILICAL cord, *TISSUE culture, *BIOLOGICAL reagents, *CELL division, *PHYSIOLOGY
Abstract

Recent studies have demonstrated that the umbilical cord (UC) is an excellent source of mesenchymal stromal cells (MSCs). However, current protocols for extracting and culturing UC-MSCs do not meet current good manufacturing practice (cGMP) standards, in part due to the use of xenogeneic reagents. To support the development of a cGMP-compliant method, we have examined an enzyme-free isolation method utilizing tissue homogenization (t-H) followed by culture in human platelet lysate (PL) supplemented media. The yield and viability of cells after t-H were comparable to those obtained after collagenase digestion (Col-D). Importantly, kinetic analysis of cultured cells showed logarithmic growth over 10 tested passages, although the rate of cell division was lower for t-H as compared to Col-D. This slower growth of t-H-derived cells was also reflected in their longer population doubling time. Interestingly, there was no difference in the expression of mesenchymal markers and trilineage differentiation potential of cells generated using either method. Finally, t-H-derived cells had greater clonogenic potential compared to Col-D/FBS but not Col-D/PL and were able to maintain CFU-F capacity through P7. This bench scale study demonstrates the possibility of generating therapeutic doses of good quality UC-MSCs within a reasonable length of time using t-H and PL. [ABSTRACT FROM AUTHOR]

Published
2016
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25. Array-Based Comparative Genomic Hybridization Identifies CDK4 and FOXM1 Alterations as Independent Predictors of Survival in Malignant Peripheral Nerve Sheath Tumor

Author

Yu, Jinsheng, primary, Deshmukh, Hrishikesh, additional, Payton, Jacqueline E., additional, Dunham, Christopher, additional, Scheithauer, Bernd W., additional, Tihan, Tarik, additional, Prayson, Richard A., additional, Guha, Abhijit, additional, Bridge, Julia A., additional, Ferner, Rosalie E., additional, Lindberg, Guy M., additional, Gutmann, Rebecca J., additional, Emnett, Ryan J., additional, Salavaggione, Lorena, additional, Gutmann, David H., additional, Nagarajan, Rakesh, additional, Watson, Mark A., additional, and Perry, Arie, additional

Published
2011
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29. Distinct Genetic Signatures among Pilocytic Astrocytomas Relate to Their Brain Region Origin

Author

Sharma, Mukesh K., primary, Mansur, David B., additional, Reifenberger, Guido, additional, Perry, Arie, additional, Leonard, Jeffrey R., additional, Aldape, Kenneth D., additional, Albin, Meredith G., additional, Emnett, Ryan J., additional, Loeser, Simon, additional, Watson, Mark A., additional, Nagarajan, Rakesh, additional, and Gutmann, David H., additional

Published
2007
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32. Conditional KIAA1549:BRAF mice reveal brain region- and cell type-specific effects.

Author

Kaul A, Chen YH, Emnett RJ, Gianino SM, and Gutmann DH

Subjects
Animals, Astrocytes cytology, Astrocytes physiology, Astrocytoma genetics, Astrocytoma pathology, Brain metabolism, Cell Proliferation, Cerebellum cytology, Child, Green Fluorescent Proteins genetics, Green Fluorescent Proteins metabolism, Humans, Mice, Mitogen-Activated Protein Kinases metabolism, Models, Animal, Neuroglia cytology, Oncogene Proteins, Fusion genetics, Organ Specificity, Mice, Transgenic, Neural Stem Cells physiology, Neuroglia physiology, Oncogene Proteins, Fusion physiology, Proto-Oncogene Proteins B-raf genetics, Proto-Oncogene Proteins B-raf metabolism
Abstract

Low-grade brain tumors (pilocytic astrocytomas) that result from a genomic rearrangement in which the BRAF kinase domain is fused to the amino terminal of the KIAA1549 gene (KIAA1549:BRAF fusion; f-BRAF) commonly arise in the cerebellum of young children. To model this temporal and spatial specificity in mice, we generated conditional KIAA1549:BRAF strains that coexpresses green fluorescent protein (GFP). Although both primary astrocytes and neural stem cells (NSCs) from these mice express f-BRAF and GFP as well as exhibit increased MEK activity, only f-BRAF-expressing NSCs exhibit increased proliferation in vitro. Using Cre driver lines in which KIAA1549:BRAF expression was directed to NSCs (f-BRAF; BLBP-Cre mice), astrocytes (f-BRAF; GFAP-Cre mice), and NG2 progenitor cells (f-BRAF; NG2-Cre mice), increased glial cell numbers were observed only in the cerebellum of f-BRAF; BLBP-Cre mice in vivo. The availability of this unique KIAA1549:BRAF conditional transgenic mouse strain will enable future mechanistic studies aimed at defining the developmentally-regulated temporal and spatial determinants that underlie low-grade astrocytoma formation in children., (Copyright © 2013 Wiley Periodicals, Inc.)

Published
2013
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