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3 results on '"Emine ŞAHİN SEMERCİ"'

1. Polymorphisms in some candidate genes associated with egg yield and quality in five different white layer pure lines

Author

Eymen DEMİR, Taki KARSLI, Hüseyin Göktuğ FİDAN, Bahar ARGUN KARSLI, Mehmet ASLAN, Sedat AKTAN, Serdar KAMANLI, Kemal KARABAĞ, Emine ŞAHİN SEMERCİ, and Murat Soner BALCIOĞLU

Subjects
candidate genes, pcr-rflp, polymorphism, pure chicken lines, Veterinary medicine, SF600-1100
Abstract

This is the first comprehensive study aimed to determine polymorphisms in candidate genes (OCX32-exon4, OCX32-exon2, GHR-intron 2, GHR-intron 5, DRD1, DRD2, VIP-501, VIP-I2, VIPR-1, VIPR-2 and MR1C) reported to associated with egg yield and quality in white layer pure chicken lines (Black, Brown, Blue, Maroon and D-229) raised by Ankara Poultry Research Institute by using PCR-RFLP technique. A total of 11 gene regions were amplificated for 192 chickens and were then digested with specific restriction endonucleases to determine the genotypes. Although all chicken lines were monomorphic for VIPR-1/HhaI and VIP-501/VspI gene region, polymorphisms were detected in the rest of nine gene regions. The present study revealed that GHR-intron 2/HindIII and GHR-intron 5/NspI polymorphsims can be integrated with MAS studies to increase egg quality in white layer chicken lines (D-229 excluded) raised in Ankara Poultry Research Institute in the future. In addition, in order to increase egg yield DRD2 and MR1C gene may be used in MAS for all studied chicken lines, while VIP-I2/HinfI polymorphism can be used for Maroon line and VIPR-2/TaqI polymorphism for Brown and D-229.

Published
2020
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2. Polymorphisms in candidate genes associated with egg yield and quality in brown layer pure lines

Author

Taki Karsli, Sedat Aktan, Serdar Kamanli, Kemal Karabağ, Hüseyin Göktuğ Fidan, Murat Soner Balcioğlu, Emine Şahin Semerci, Mehmet Aslan, Eymen Demir, and Bahar Argun Karsli

Subjects
Candidate gene, Horticulture, Yield (engineering), Quality (physics), General Medicine, Biology, Layer (electronics)
Abstract

The aim of the present study was to detect a total of 11 polymorphisms of 6 genes (OCX32, GHR, DRD, VIP, VIPR, MR) related to egg yield and quality in six brown layer lines namely Rhode Island Red-I (RIRI), Rhode Island Red-II (RIRII), Barred Rock-I (BARI), Barred Rock-II (BARII), Colombian Rock (COL) and Line-54 (L5-4) raised in Ankara Poultry Research Institute. A total of 208 samples belonging to RIRI (n= 32), RIRII (n= 32), BARI (n= 40), BARII (n= 32), COL (n= 32) and L-54 (n= 40) were genotyped by PCR-RFLP method. For this reason, a total of 11 polymorphic regions were amplified by PCR and then PCR products were digested with specific restriction endonucleases. Results of the present study showed that polymorphisms and adequate genetic variations were found in OCX32-ex4/NcoI, OCX32-ex2/HpyCH4IV, DRD2/BseGI, VIPR-1/HhaI, VIPR-2/TaqI and MR1C/MboI genes which previously reported to associated with egg quality and yield in different chicken lines. However, conducting the association analysis between these genes and egg yield and quality will be useful. On the contrary, it has been revealed that GHR-intron 2/HindIII, GHR-intron 5/NspI, VIP-501/VspI, DRD1/BseNI and VIP-I2/HinfI polymorphisms cannot be used to increase egg yield and quality due to no detection of desired genotypes or homozygous excess.

Published
2020
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3. Determination of genetic variability, population structure and genetic differentiation of indigenous Turkish goat breeds based on SSR loci

Author

Eymen Demir, Mehmet Aslan, Murat Soner Balcıoğlu, Ibrahim Zafer Arik, Taki Karsli, Emine Şahin Semerci, Kemal Karabağ, Hüseyin Göktuğ Fidan, and Bahar Argun Karsli

Subjects
integumentary system, Phylogenetic tree, 040301 veterinary sciences, Turkish, Population structure, 0402 animal and dairy science, 04 agricultural and veterinary sciences, Biology, 040201 dairy & animal science, Breed, language.human_language, 0403 veterinary science, Loss of heterozygosity, Food Animals, Evolutionary biology, language, Microsatellite, Animal Science and Zoology, Genetic variability, Allele
Abstract

The present study aimed to determine genetic variability and population structure of native Turkish goats including Hair, Kabakulak, Honamli and Norduz. A total of 255 different alleles and 16 private alleles were detected across 20 microsatellite loci. Number of alleles ranged from 8.30 (Norduz) to 11.70 (Hair), whereas number of effective alleles varied from 5.20 (Norduz) to 7.21 (Hair). Observed heterozygosity ranged from 0.69 (Honamli) to 0.75 (Hair), while expected heterozygosity varied from 0.80 (Norduz) to 0.85 (Hair). FIS values ranged from 0.09 (HAI) to 0.16 (HNM). Unlike previous studies, phylogenetic analysis revealed that Norduz breed was turned out to be genetically different from the other breeds for the first time. Additionally, the present study was the first attempt revealing that Kabakulak goats which are accepted as Hair variety until today, were genetically closer to Honamli than Hair goat breed.

Published
2020
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