30 results on '"Emiliane Taillebois"'
Search Results
2. Effect of Acetamiprid, a Neonicotinoid Insecticide, on Locomotor Activity of the American Cockroach
- Author
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Emiliane Taillebois, Alison Cartereau, and Steeve H. Thany
- Subjects
insect ,cockroach ,locomotor activity ,insecticide ,neonicotinoid ,acetamiprid ,Science - Abstract
Toxicological studies have shown that the American cockroach Periplaneta americana (Linnaeus) is a classical model for studying the mode of action of commonly used insecticides. In a previous study, we demonstrated that thiamethoxam and clothianidin decreased locomotor activity in an open-field-like apparatus. Here, we tested the effect of the neonicotinoid acetamiprid when applied orally, topically, or injected into the haemolymph. We found that acetamiprid was also able to impair locomotor activity in the open-field-like apparatus. When treated with acetamiprid, a strong alteration in locomotor activity was observed 1 h, 24 h, and 48 h after haemolymph and topical applications. Oral application induced an impairment of locomotor activity at 24 h and 48 h. A comparison of the present data with our previously published results showed that neonicotinoids were more active when injected into the haemolymph compared to oral and topical applications. These findings increased our understanding of the effect of neonicotinoid insecticides on insect locomotor activity, and demonstrated that the cyano-substituted neonicotinoid, acetamiprid, was able to alter cockroach locomotor activity.
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- 2024
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3. Transcriptome of the synganglion in the tick Ixodes ricinus and evolution of the cys-loop ligand-gated ion channel family in ticks
- Author
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Claude Rispe, Caroline Hervet, Nathalie de la Cotte, Romain Daveu, Karine Labadie, Benjamin Noel, Jean-Marc Aury, Steeve Thany, Emiliane Taillebois, Alison Cartereau, Anaïs Le Mauff, Claude L. Charvet, Clément Auger, Elise Courtot, Cédric Neveu, and Olivier Plantard
- Subjects
Cys-loop receptors ,Tick ,Synganglion ,Differential expression ,Duplication ,Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background Ticks represent a major health issue for humans and domesticated animals. Exploring the expression landscape of the tick’s central nervous system (CNS), known as the synganglion, would be an important step in understanding tick physiology and in managing tick-borne diseases, but studies on that topic are still relatively scarce. Neuron-specific genes like the cys-loop ligand-gated ion channels (cys-loop LGICs, or cysLGICs) are important pharmacological targets of acaricides. To date their sequence have not been well catalogued for ticks, and their phylogeny has not been fully studied. Results We carried out the sequencing of transcriptomes of the I. ricinus synganglion, for adult ticks in different conditions (unfed males, unfed females, and partially-fed females). The de novo assembly of these transcriptomes allowed us to obtain a large collection of cys-loop LGICs sequences. A reference meta-transcriptome based on synganglion and whole body transcriptomes was then produced, showing high completeness and allowing differential expression analyses between synganglion and whole body. Many of the genes upregulated in the synganglion were associated with neurotransmission and/or localized in neurons or the synaptic membrane. As the first step of a functional study of cysLGICs, we cloned the predicted sequence of the resistance to dieldrin (RDL) subunit homolog, and functionally reconstituted the first GABA-gated receptor of Ixodes ricinus. A phylogenetic study was performed for the nicotinic acetylcholine receptors (nAChRs) and other cys-loop LGICs respectively, revealing tick-specific expansions of some types of receptors (especially for Histamine-like subunits and GluCls). Conclusions We established a large catalogue of genes preferentially expressed in the tick CNS, including the cysLGICs. We discovered tick-specific gene family expansion of some types of cysLGIC receptors, and a case of intragenic duplication, suggesting a complex pattern of gene expression among different copies or different alternative transcripts of tick neuro-receptors.
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- 2022
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4. Effect of the combination of DEET and flupyradifurone on the tick Ixodes ricinus: Repellency bioassay and pharmacological characterization using microtransplantation of synganglion membranes
- Author
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Anaïs Le Mauff, Alison Cartereau, Olivier Plantard, Emiliane Taillebois, and Steeve H. Thany
- Subjects
Ixodes ricinus ,DEET ,Flupyradifurone ,Synganglion membranes ,Nicotinic receptors ,Infectious and parasitic diseases ,RC109-216 - Abstract
Ticks are vectors of many human and animal pathogens, and represent a major threat to public health. In recent years, an increase in tick-borne diseases has been observed, and new strategies are therefore needed in order to control tick numbers and reduce human tick bites. In the present study, we adapted the previous tick repellency bioassay based on the exploration behavior of the tick, using the ToxTrac software and video-tracking, to compare the repellent effect of two compounds on the tick Ixodes ricinus: N,N-diethyl-methyl-m-toluamide (DEET), and butenolide, flupyradifurone (FLU). We found that when applied alone, 10% DEET or FLU have no/or low repellency effect. But, the combination of both 10% DEET and FLU demonstrated a significant repellency effect against I. ricinus, similar to the repellency of 20% DEET. Using membrane microtransplantation, we evaluated the effect of DEET and FLU on native acetylcholine receptors expressed on the tick synganglion. We found that DEET has no effect on acetylcholine-evoked currents, but significantly reduced nicotine-induced current amplitudes. FLU induced an ionic current but was not able to reduce acetylcholine or nicotine evoked currents. The combination of both DEET and FLU strongly reduced nicotine-evoked currents. Finally, we demonstrated that our recording device for repellency, as well as the use of membrane microtransplantation, could be used as methods to study the mode of action of active compounds on ticks.
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- 2023
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- View/download PDF
5. Nicotinic acetylcholine receptors in the synganglion of the tick Ixodes ricinus: Functional characterization using membrane microtransplantation
- Author
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Anaïs Le Mauff, Hamza Chouikh, Alison Cartereau, Claude L. Charvet, Cédric Neveu, Claude Rispe, Olivier Plantard, Emiliane Taillebois, and Steeve H. Thany
- Subjects
Tick ,Ixodes ricinus ,Synganglion membrane ,Microtransplantation ,Nicotinic acetylcholine receptors ,Infectious and parasitic diseases ,RC109-216 - Abstract
Nicotinic acetylcholine receptors are an important class of excitatory receptors in the central nervous system of arthropods. In the ticks Ixodes ricinus, the functional and pharmacological properties of nicotinic receptors located in their neurons are still unknown. The objective of this study was to characterize the pharmacological properties of tick nicotinic receptors using membrane microtransplantation in Xenopus laevis oocytes and two-electrodes voltage clamp method. The membranes microtransplanted were extracted from the tick synganglion. We found that oocytes microtransplanted with tick synganglion membranes expressed nicotinic acetylcholine receptor subtypes which were activated by acetylcholine (1 mM) and nicotine (1 mM). Currents induced by pressure application of acetylcholine and nicotine were diminished by 10 nM α-bungarotoxin and methyllycaconitine, suggesting that they expressed two subtypes of nicotinic receptors, α-bungarotoxin-sensitive and -insensitive, respectively. In addition, we found that nicotine receptors expressed in the synganglion membranes were poorly sensitive to the neonicotinoid insecticides clothianidin (CLT), imidacloprid (IMI), acetamiprid (ACE) and thiamethoxam (TMX), in agreement with their lack of activity as acaricides. Interestingly, current amplitudes were strongly potentialized in the presence of 1 μM PNU-120596. CLT was more active as an agonist than IMI, TMX and ACE. Finally, we demonstrated that microtransplantation of purified membrane from the tick synganglion can be a valuable tool for the development and screening of compounds targeting tick nicotinic acetylcholine receptor subtypes.
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- 2020
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6. Impairments in learning and memory performances associated with nicotinic receptor expression in the honeybee Apis mellifera after exposure to a sublethal dose of sulfoxaflor
- Author
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Alison Cartereau, Xavier Pineau, Jacques Lebreton, Monique Mathé-Allainmat, Emiliane Taillebois, and Steeve H. Thany
- Subjects
Medicine ,Science - Abstract
Sulfoxaflor is a new insecticide which acts on the nicotinic acetylcholine receptor (nAChRs) in a similar way to neonicotinoids. However, sufloxaflor (SFX) is thought to act in a different manner and is thus proposed as an alternative in crop protection. The goal of this study is to evaluate the toxicity of SFX and its sublethal effect on the honeybee Apis mellifera after acute exposure. In toxicological assay studies, the LD50 value and sublethal dose (corresponding to the NOEL: no observed effect level) were 96 and 15 ng/bee, respectively. Using the proboscis extension response paradigm, we found that an SFX dose of 15 ng/bee significantly impairs learning and memory retrieval when applied 12 h before conditioning or 24 h after olfactory conditioning. SFX had no effect on honeybee olfactory performance when exposure happened after the conditioning. Relative quantitative PCR experiments performed on the six nicotinic acetylcholine receptor subunits demonstrated that they are differently expressed in the honeybee brain after SFX exposure, whether before or after conditioning. We found that intoxicated bees with learning defects showed a strong expression of the Amelβ1 subunit. They displayed overexpression of Amelα9 and Amelβ2, and down-regulation of Amelα1, Amelα3 and Amelα7 subunits. These results demonstrated for the first time that a sublethal dose of SFX could affect honeybee learning and memory performance and modulate the expression of specific nAChR subunits in the brain.
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- 2022
7. Correction: Transcriptome of the synganglion in the tick Ixodes ricinus and evolution of the cys-loop ligand-gated ion channel family in ticks
- Author
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Claude Rispe, Caroline Hervet, Nathalie de la Cotte, Romain Daveu, Karine Labadie, Benjamin Noel, Jean-Marc Aury, Steeve Thany, Emiliane Taillebois, Alison Cartereau, Anaïs Le Mauf, Claude L. Charvet, Clément Auger, Elise Courtot, Cédric Neveu, and Olivier Plantard
- Subjects
Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Published
- 2022
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8. Cloning and Expression of Cockroach α7 Nicotinic Acetylcholine Receptor Subunit
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Alison Cartereau, Emiliane Taillebois, Balaji Selvam, Carine Martin, Jérôme Graton, Jean-Yves Le Questel, and Steeve H. Thany
- Subjects
nicotinic receptor ,insect ,α7 subunit ,acetylcholine ,nicotine ,neonicotinoid ,Physiology ,QP1-981 - Abstract
Understanding insect nicotinic acetylcholine receptor (nAChR) subtypes is of major interest because they are the main target of several insecticides. In this study, we have cloned a cockroach Pameα7 subunit that encodes a 518 amino acid protein with futures typical of nAChR subunit, and sequence homology to α7 subunit. Pameα7 is differently expressed in the cockroach nervous system, in particular in the antennal lobes, optical lobes and the mushroom bodies where specific expression was found in the non-compact Kenyon cells. In addition, we found that cockroach Pameα7 subunits expressed in Xenopus laevis oocytes can assemble to form homomeric receptors. Electrophysiological recordings using the two-electrode voltage clamp method demonstrated that nicotine induced an Imax current of −92 ± 27 nA at 1 mM. Despite that currents are low with the endogenous ligand, ACh, this study provides information on the first expression of cockroach α7 homomeric receptor.
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- 2020
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9. Mode of Action of Neonicotinoid Insecticides Imidacloprid and Thiacloprid to the Cockroach Pameα7 Nicotinic Acetylcholine Receptor
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Alison Cartereau, Emiliane Taillebois, Jean-Yves Le Questel, and Steeve H. Thany
- Subjects
acetylcholine ,nicotinic receptors ,neonicotinoids ,imidacloprid ,thiacloprid ,molecular modeling ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
The functional expression of the cockroach Pameα7 nicotinic acetylcholine receptor subunit has been previously studied, and was found to be able to form a homomeric receptor when expressed in Xenopus laevis oocytes. In this study, we found that the neonicotinoid insecticide imidacloprid is unable to activate the cockroach Pameα7 receptor, although thiacloprid induces low inward currents, suggesting that it is a partial agonist. In addition, the co-application or 5 min pretreatment with 10 µM imidacloprid increased nicotine current amplitudes, while the co-application or 5 min pretreatment with 10 µM thiacloprid decreased nicotine-evoked current amplitudes by 54% and 28%, respectively. This suggesting that these two representatives of neonicotinoid insecticides bind differently to the cockroach Pameα7 receptor. Interestingly, the docking models demonstrate that the orientation and interactions of the two insecticides in the cockroach Pameα7 nAChR binding pocket are very similar. Electrophysiological results have provided evidence to suggest that imidacloprid and thiacloprid could act as modulators of the cockroach Pameα7 receptors.
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- 2021
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10. Characterisation of adipocyte-derived extracellular vesicle subtypes identifies distinct protein and lipid signatures for large and small extracellular vesicles
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Maëva Durcin, Audrey Fleury, Emiliane Taillebois, Grégory Hilairet, Zuzana Krupova, Céline Henry, Sandrine Truchet, Martin Trötzmüller, Harald Köfeler, Guillaume Mabilleau, Olivier Hue, Ramaroson Andriantsitohaina, Patrice Martin, and Soazig Le Lay
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Extracellular vesicles ,exosomes ,microvesicles ,large EVs ,small EVs ,adipocytes ,Cytology ,QH573-671 - Abstract
Extracellular vesicles (EVs) are biological vectors that can modulate the metabolism of target cells by conveying signalling proteins and genomic material. The level of EVs in plasma is significantly increased in cardiometabolic diseases associated with obesity, suggesting their possible participation in the development of metabolic dysfunction. With regard to the poor definition of adipocyte-derived EVs, the purpose of this study was to characterise both qualitatively and quantitatively EVs subpopulations secreted by fat cells. Adipocyte-derived EVs were isolated by differential centrifugation of conditioned media collected from 3T3-L1 adipocytes cultured for 24 h in serum-free conditions. Based on morphological and biochemical properties, as well as quantification of secreted EVs, we distinguished two subpopulations of adipocyte-derived EVs, namely small extracellular vesicles (sEVs) and large extracellular vesicles (lEVs). Proteomic analyses revealed that lEVs and sEVs exhibit specific protein signatures, allowing us not only to define novel markers of each population, but also to predict their biological functions. Despite similar phospholipid patterns, the comparative lipidomic analysis performed on these EV subclasses revealed a specific cholesterol enrichment of the sEV population, whereas lEVs were characterised by high amounts of externalised phosphatidylserine. Enhanced secretion of lEVs and sEVs is achievable following exposure to different biological stimuli related to the chronic low-grade inflammation state associated with obesity. Finally, we demonstrate the ability of primary murine adipocytes to secrete sEVs and lEVs, which display physical and biological characteristics similar to those described for 3T3-L1. Our study provides additional information and elements to define EV subtypes based on the characterisation of adipocyte-derived EV populations. It also underscores the need to distinguish EV subpopulations, through a combination of multiple approaches and markers, since their specific composition may cause distinct metabolic responses in recipient cells and tissues.
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- 2017
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11. Neonicotinoid binding, toxicity and expression of nicotinic acetylcholine receptor subunits in the aphid Acyrthosiphon pisum.
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Emiliane Taillebois, Abdelhamid Beloula, Sophie Quinchard, Stéphanie Jaubert-Possamai, Antoine Daguin, Denis Servent, Denis Tagu, Steeve H Thany, and Hélène Tricoire-Leignel
- Subjects
Medicine ,Science - Abstract
Neonicotinoid insecticides act on nicotinic acetylcholine receptor and are particularly effective against sucking pests. They are widely used in crops protection to fight against aphids, which cause severe damage. In the present study we evaluated the susceptibility of the pea aphid Acyrthosiphon pisum to the commonly used neonicotinoid insecticides imidacloprid (IMI), thiamethoxam (TMX) and clothianidin (CLT). Binding studies on aphid membrane preparations revealed the existence of high and low-affinity binding sites for [3H]-IMI (Kd of 0.16 ± 0.04 nM and 41.7 ± 5.9 nM) and for the nicotinic antagonist [125I]-α-bungarotoxin (Kd of 0.008 ± 0.002 nM and 1.135 ± 0.213 nM). Competitive binding experiments demonstrated that TMX displayed a higher affinity than IMI for [125I]-α-bungarotoxin binding sites while CLT affinity was similar for both [125I]-α-bungarotoxin and [3H]-IMI binding sites. Interestingly, toxicological studies revealed that at 48 h, IMI (LC50 = 0.038 µg/ml) and TMX (LC50 = 0.034 µg/ml) were more toxic than CLT (LC50 = 0.118 µg/ml). The effect of TMX could be associated to its metabolite CLT as demonstrated by HPLC/MS analysis. In addition, we found that aphid larvae treated either with IMI, TMX or CLT showed a strong variation of nAChR subunit expression. Using semi-quantitative PCR experiments, we detected for all insecticides an increase of Apisumα10 and Apisumβ1 expressions levels, whereas Apisumβ2 expression decreased. Moreover, some other receptor subunits seemed to be differently regulated according to the insecticide used. Finally, we also demonstrated that nAChR subunit expression differed during pea aphid development. Altogether these results highlight species specificity that should be taken into account in pest management strategies.
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- 2014
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12. The Differential Effect of Low-Dose Mixtures of Four Pesticides on the Pea Aphid Acyrthosiphon pisum
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Emiliane Taillebois and Steeve H. Thany
- Subjects
aphid ,neonicotinoid ,organophosphate ,pyrethroid ,phenylpyrazol ,Science - Abstract
The modes of action of most insecticides are known, but little information exists regarding the toxicological interactions involving insecticide mixtures at low doses. The effects of mixtures of four insecticides were investigated using LC10 values (concentration leading to 10% mortality), acetamiprid (ACE, 0.235 µg/mL), chlorpyriphos (CHL, 107.0 µg/mL), deltamethrin (DEL, 5.831 µg/mL), and fipronil (FIP, 3.775 µg/mL) on the larvae of the pea aphid, Acyrthosiphon pisum. After 24 h exposure, 6 of the 11 tested combinations, DEL/FIP, ACE/DEL, CHL/FIP, ACE/DEL/FIP, ACE/CHL/FIP, and ACE/DEL/CHL/FIP, were toxic through an additive effect. Four combinations, ACE/FIP, DEL/CHL, ACE/CHL, and ACE/DEL/CHL had a synergistic effect, whereas only one DEL/CHL/FIP showed an antagonistic effect. The toxic effect of these mixtures was confirmed after 48 h of exposure, revealing an enhanced toxicity of CHL, DEL, and FIP in combination with ACE. We suggest that an insect pest management strategy should be evaluated in the future using different combinations of insecticides.
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- 2016
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13. Effect of the combination of DEET and flupyradifurone on the tick Ixodes ricinus: Repellency bioassay and pharmacological characterization using microtransplantation of synganglion membranes
- Author
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Anaïs Le Mauff, Alison Cartereau, Olivier Plantard, Emiliane Taillebois, and Steeve H. Thany
- Subjects
Infectious Diseases ,Insect Science ,Parasitology ,Microbiology - Abstract
Ticks are vectors of many human and animal pathogens, and represent a major threat to public health. In recent years, an increase in tick-borne diseases has been observed, and new strategies are therefore needed in order to control tick numbers and reduce human tick bites. In the present study, we adapted the previous tick repellency bioassay based on the exploration behavior of the tick, using the ToxTrac software and video-tracking, to compare the repellent effect of two compounds on the tick Ixodes ricinus: N,N-diethyl-methyl-m-toluamide (DEET), and butenolide, flupyradifurone (FLU). We found that when applied alone, 10% DEET or FLU have no/or low repellency effect. But, the combination of both 10% DEET and FLU demonstrated a significant repellency effect against I. ricinus, similar to the repellency of 20% DEET. Using membrane microtransplantation, we evaluated the effect of DEET and FLU on native acetylcholine receptors expressed on the tick synganglion. We found that DEET has no effect on acetylcholine-evoked currents, but significantly reduced nicotine-induced current amplitudes. FLU induced an ionic current but was not able to reduce acetylcholine or nicotine evoked currents. The combination of both DEET and FLU strongly reduced nicotine-evoked currents. Finally, we demonstrated that our recording device for repellency, as well as the use of membrane microtransplantation, could be used as methods to study the mode of action of active compounds on ticks.
- Published
- 2022
14. The use of insecticide mixtures containing neonicotinoids as a strategy to limit insect pests: Efficiency and mode of action
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Steeve THANY and Emiliane Taillebois
- Subjects
Insecticide Resistance ,Insecticides ,Neonicotinoids ,Insecta ,Health, Toxicology and Mutagenesis ,Animals ,General Medicine ,Receptors, Nicotinic ,Agronomy and Crop Science - Abstract
Synthetic insecticides continue to be the main strategy for managing insect pests, which are a major concern for both crop protection and public health. As nicotinic acetylcholine receptors play a central role in insect neurotransmission, they are the molecular target of neurotoxic insecticides such as neonicotinoids. These insecticides are used worldwide and have shown high efficiency in culture protection. However, the emergence of insect resistance mechanisms, and negative side-effects on non-target species have highlighted the need for a new control strategy. In this context, the use of insecticide mixtures with synergistic effects have been used in order to decrease the insecticide dose, and thus delay the selection of resistance-strains, and limit their negative impact. In this review, we summarize the available data concerning the mode of action of neonicotinoid mixtures, as well as their toxicity to various insect pests and non-target species. We found that insecticide mixtures containing neonicotinoids may be an effective strategy for limiting insect pests, and in particular resistant strains, although they could also negatively impact non-target species such as pollinating insects.
- Published
- 2022
15. Transcriptome of the synganglion and characterization of the cys-loop ligand-gated ion channel gene family in the tick Ixodes ricinus
- Author
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Claude Rispe, Caroline Hervet, Nathalie de la Cotte, Romain Daveu, Karine Labadie, Benjamin Noël, Jean-Marc Aury, Steeve Thany, Emiliane Taillebois, Alison Cartereau, Anaïs Le Mauff, Claude L. Charvet, Clément Auger, Cédric Neveu, and Olivier Plantard
- Abstract
Ticks represent a major health issue for humans and domesticated animals. Assessing the expression patterns of the tick’s central nervous system, known as the synganglion, is an important step in understanding tick physiology and in managing tick-borne diseases. Neuron-specific genes like the cys-loop ligand-gated ion channels (cys-loop LGICs) are important pharmacological targets of acaricides. Here, we carried out the sequencing of transcriptomes of the I. ricinus synganglion, for adult ticks in different conditions (unfed males, unfed females, and partially-fed females). The de novo assembly of these transcriptomes allowed us to obtain a large collection of cys-loop LGICs sequences. A reference meta-transcriptome based on synganglion and whole body transcriptomes was then produced, showing high completeness and allowing differential expression analyses between synganglion and whole body. Many of the genes upregulated in the synganglion were related to biological processes or functions associated with neurotransmission and located in neurones or the synaptic membrane, including most of the cys-loop LGICs. As a first step of a functional study of cysLGICs, we cloned the predicted sequence of the resistance to dieldrin (RDL) subunit homologue, and functionally reconstituted the first GABA-gated receptor of Ixodes ricinus using a hetrologous expression approach. A phylogenetic study was performed for the nicotinic acetylcholine receptors (nAChRs) and for other cys-loop LGICs respectively, showing tick-specific expansions of some types of receptors (Histamine-gated, GluCls).
- Published
- 2021
16. Impairments in learning and memory performances associated with nicotinic receptor expression in the honeybee Apis mellifera after exposure to a sublethal dose of sulfoxaflor
- Author
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Alison, Cartereau, Xavier, Pineau, Jacques, Lebreton, Monique, Mathé-Allainmat, Emiliane, Taillebois, and Steeve H, Thany
- Subjects
Insecticides ,Neonicotinoids ,Sulfur Compounds ,Pyridines ,Animals ,Learning ,Bees ,Receptors, Nicotinic - Abstract
Sulfoxaflor is a new insecticide which acts on the nicotinic acetylcholine receptor (nAChRs) in a similar way to neonicotinoids. However, sufloxaflor (SFX) is thought to act in a different manner and is thus proposed as an alternative in crop protection. The goal of this study is to evaluate the toxicity of SFX and its sublethal effect on the honeybee Apis mellifera after acute exposure. In toxicological assay studies, the LD50 value and sublethal dose (corresponding to the NOEL: no observed effect level) were 96 and 15 ng/bee, respectively. Using the proboscis extension response paradigm, we found that an SFX dose of 15 ng/bee significantly impairs learning and memory retrieval when applied 12 h before conditioning or 24 h after olfactory conditioning. SFX had no effect on honeybee olfactory performance when exposure happened after the conditioning. Relative quantitative PCR experiments performed on the six nicotinic acetylcholine receptor subunits demonstrated that they are differently expressed in the honeybee brain after SFX exposure, whether before or after conditioning. We found that intoxicated bees with learning defects showed a strong expression of the Amelβ1 subunit. They displayed overexpression of Amelα9 and Amelβ2, and down-regulation of Amelα1, Amelα3 and Amelα7 subunits. These results demonstrated for the first time that a sublethal dose of SFX could affect honeybee learning and memory performance and modulate the expression of specific nAChR subunits in the brain.
- Published
- 2021
17. Mode of Action of Neonicotinoid Insecticides Imidacloprid and Thiacloprid to the Cockroach Pameα7 Nicotinic Acetylcholine Receptor
- Author
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Steeve H. Thany, Jean-Yves Le Questel, Alison Cartereau, and Emiliane Taillebois
- Subjects
Insecticides ,nicotinic receptors ,Patch-Clamp Techniques ,binding studies ,QH301-705.5 ,Thiazines ,Cockroaches ,Nicotinic Antagonists ,Receptors, Nicotinic ,Pharmacology ,Partial agonist ,Article ,Catalysis ,Inorganic Chemistry ,Xenopus laevis ,chemistry.chemical_compound ,Imidacloprid ,biology.animal ,medicine ,Animals ,Nicotinic Agonists ,Biology (General) ,Physical and Theoretical Chemistry ,Receptor ,QD1-999 ,Molecular Biology ,Spectroscopy ,Cockroach ,biology ,molecular modeling ,Organic Chemistry ,Neonicotinoid ,General Medicine ,imidacloprid ,Nitro Compounds ,Thiacloprid ,acetylcholine ,Computer Science Applications ,Chemistry ,Nicotinic acetylcholine receptor ,chemistry ,Oocytes ,neonicotinoids ,thiacloprid ,Acetylcholine ,medicine.drug - Abstract
The functional expression of the cockroach Pameα7 nicotinic acetylcholine receptor subunit has been previously studied, and was found to be able to form a homomeric receptor when expressed in Xenopus laevis oocytes. In this study, we found that the neonicotinoid insecticide imidacloprid is unable to activate the cockroach Pameα7 receptor, although thiacloprid induces low inward currents, suggesting that it is a partial agonist. In addition, the co-application or 5 min pretreatment with 10 µM imidacloprid increased nicotine current amplitudes, while the co-application or 5 min pretreatment with 10 µM thiacloprid decreased nicotine-evoked current amplitudes by 54% and 28%, respectively. This suggesting that these two representatives of neonicotinoid insecticides bind differently to the cockroach Pameα7 receptor. Interestingly, the docking models demonstrate that the orientation and interactions of the two insecticides in the cockroach Pameα7 nAChR binding pocket are very similar. Electrophysiological results have provided evidence to suggest that imidacloprid and thiacloprid could act as modulators of the cockroach Pameα7 receptors.
- Published
- 2021
- Full Text
- View/download PDF
18. Calmidazolium induces a decrease in nicotine-induced currents and intracellular calcium levels after pulse application of nicotine onto insect neurosecretory cells
- Author
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Jean-Noël Houchat, Alison Cartereau, Emiliane Taillebois, and Steeve H. Thany
- Subjects
Mammals ,Nicotine ,Insecta ,Physiology ,Insect Science ,Imidazoles ,Animals ,Calcium ,Cockroaches ,Bungarotoxins - Abstract
Dorsal unpaired median (DUM) neurons, are a class of insect neurosecretory cells, which are involved in the control of several functions, such as excretion and reproduction, or the release of neurohormones. Previous studies demonstrated that they express different nicotinic acetylcholine receptor subtypes, in particular α-bungarotoxin-insensitive receptors, with nAChR1 and nAChR2 subtypes. Here, we demonstrated that pulse application of 1 mM nicotine (300 ms pulse duration) induced inward currents which were reduced under bath application of 15 µM calmidazolium, a calmodulin inhibitor. Bath application of 0.5 µM α-bungarotoxin had no effect on calmidazolium action, suggesting that it could have an indirect effect through α-bungarotoxin-insensitive receptors. Indeed, nicotine-evoked currents were reduced by 10 µM d-tubocurarine, and completely blocked by 5 µM mecamylamine, which affected nAChR1 and nAChR2 subtypes, respectively. Our results demonstrated that nAChR2 subtypes are involved in the indirect effect of calmidazolium. Moreover, we found that this calmidazolium effect was associated to a strong reduction in intracellular calcium levels after pulse application of 1 mM nicotine. Thus, compared to previous studies on mammalian cells, calmidazolium did not cause an increase in intracellular calcium levels in DUM neurons, suggesting that different calcium mechanisms are involved in the calmidazolium effect.
- Published
- 2022
19. Cloning and Expression of Cockroach α7 Nicotinic Acetylcholine Receptor Subunit
- Author
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Jean-Yves Le Questel, Alison Cartereau, Emiliane Taillebois, Carine Martin, Jérôme Graton, Balaji Selvam, Steeve H. Thany, Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), and Université d'Orléans (UO)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
- Subjects
0301 basic medicine ,Physiology ,Protein subunit ,[SDV]Life Sciences [q-bio] ,Xenopus ,α7 subunit ,lcsh:Physiology ,03 medical and health sciences ,0302 clinical medicine ,Physiology (medical) ,biology.animal ,medicine ,Homomeric ,Receptor ,ComputingMilieux_MISCELLANEOUS ,Original Research ,Cockroach ,lcsh:QP1-981 ,biology ,Chemistry ,neonicotinoid ,biology.organism_classification ,acetylcholine ,3. Good health ,Cell biology ,Nicotinic acetylcholine receptor ,030104 developmental biology ,Mushroom bodies ,insect ,nicotinic receptor ,030217 neurology & neurosurgery ,Acetylcholine ,nicotine ,medicine.drug - Abstract
Understanding insect nicotinic acetylcholine receptor (nAChR) subtypes is of major interest because they are the main target of several insecticides. In this study, we have cloned a cockroach Pameα7 subunit that encodes a 518 amino acid protein with futures typical of nAChR subunit, and sequence homology to α7 subunit. Pameα7 is differently expressed in the cockroach nervous system, in particular in the antennal lobes, optical lobes and the mushroom bodies where specific expression was found in the non-compact Kenyon cells. In addition, we found that cockroach Pameα7 subunits expressed in Xenopus laevis oocytes can assemble to form homomeric receptors. Electrophysiological recordings using the two-electrode voltage clamp method demonstrated that nicotine induced an I max current of -92 ± 27 nA at 1 mM. Despite that currents are low with the endogenous ligand, ACh, this study provides information on the first expression of cockroach α7 homomeric receptor.
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- 2020
20. An Overview on the Effect of Neonicotinoid Insecticides on Mammalian Cholinergic Functions through the Activation of Neuronal Nicotinic Acetylcholine Receptors
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Anaïs Le Mauff, Alison Cartereau, Jean-Noël Houchat, Steeve H. Thany, Emiliane Taillebois, Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), and Université d'Orléans (UO)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
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nicotinic receptors ,Insecticides ,Health, Toxicology and Mutagenesis ,[SDV]Life Sciences [q-bio] ,cholinergic functions ,Cholinergic Agents ,lcsh:Medicine ,Review ,Biology ,Neurotransmission ,Receptors, Nicotinic ,03 medical and health sciences ,chemistry.chemical_compound ,Neonicotinoids ,0302 clinical medicine ,medicine ,Homomeric ,neonicotinoid insecticides ,Animals ,Humans ,030304 developmental biology ,Acetylcholine receptor ,Mammals ,0303 health sciences ,lcsh:R ,Public Health, Environmental and Occupational Health ,Neonicotinoid ,Neurotoxicity ,Clothianidin ,medicine.disease ,3. Good health ,Rats ,modulation ,Nicotinic agonist ,chemistry ,nervous system ,Cholinergic ,ACh ,Neuroscience ,030217 neurology & neurosurgery - Abstract
International audience; Neonicotinoid insecticides are used worldwide and have been demonstrated as toxic to beneficial insects such as honeybees. Their effectiveness is predominantly attributed to their high affinity for insect neuronal nicotinic acetylcholine receptors (nAChRs). Mammalian neuronal nAChRs are of major importance because cholinergic synaptic transmission plays a key role in rapid neurotransmission, learning and memory processes, and neurodegenerative diseases. Because of the low agonist effects of neonicotinoid insecticides on mammalian neuronal nAChRs, it has been suggested that they are relatively safe for mammals, including humans. However, several lines of evidence have demonstrated that neonicotinoid insecticides can modulate cholinergic functions through neuronal nAChRs. Major studies on the influence of neonicotinoid insecticides on cholinergic functions have been conducted using nicotine low-affinity homomeric α7 and high-affinity heteromeric α4β2 receptors, as they are the most abundant in the nervous system. It has been found that the neonicotinoids thiamethoxam and clothianidin can activate the release of dopamine in rat striatum. In some contexts, such as neurodegenerative diseases, they can disturb the neuronal distribution or induce oxidative stress, leading to neurotoxicity. This review highlights recent studies on the mode of action of neonicotinoid insecticides on mammalian neuronal nAChRs and cholinergic functions.
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- 2020
21. Neonicotinoid insecticides mode of action on insect nicotinic acetylcholine receptors using binding studies
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Andrew K. Jones, Emiliane Taillebois, Steeve H. Thany, Alison Cartereau, Université Francois Rabelais [Tours], Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), Institut National de la Recherche Agronomique (INRA)-Université d'Orléans (UO), and Oxford Brookes University
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0301 basic medicine ,nicotinic receptors ,Insecticides ,[SDV.BIO]Life Sciences [q-bio]/Biotechnology ,binding studies ,Health, Toxicology and Mutagenesis ,media_common.quotation_subject ,Insect ,Receptors, Nicotinic ,Neonicotinoids ,03 medical and health sciences ,0302 clinical medicine ,neonicotinoid insecticides ,Animals ,Humans ,Binding site ,Mode of action ,media_common ,Acetylcholine receptor ,Binding Sites ,Chemistry ,[SDV.BA]Life Sciences [q-bio]/Animal biology ,Binding properties ,Neonicotinoid ,General Medicine ,3. Good health ,030104 developmental biology ,Nicotinic agonist ,nervous system ,Biochemistry ,insect ,Whole body ,Agronomy and Crop Science ,030217 neurology & neurosurgery ,Protein Binding - Abstract
International audience; Nicotinic acetylcholine receptors (nAChRs) are the main target of neonicotinoid insecticides, which are widely used in crop protection against insect pests. Electrophysiological and molecular approaches have demonstrated the presence of several nAChR subtypes with different affinities for neonicotinoid insecticides. However, the precise mode of action of neonicotinoids on insect nAChRs remains to be elucidated. Radioligand binding studies with [3H]-α-bungarotoxin and [3H]-imidacloprid have proved instructive in understanding ligand binding interactions between insect nAChRs and neonicotinoid insecticides. The precise binding site interactions have been established using membranes from whole body and specific tissues. In this review, we discuss findings concerning the number of nAChR binding sites against neonicotinoid insecticides from radioligand binding studies on native tissues. We summarize the data available in the literature and compare the binding properties of the most commonly used neonicotinoid insecticides in several insect species. Finally, we demonstrate that neonicotinoid-nAChR binding sites are also linked to biological samples used and insect species.
- Published
- 2018
22. CHARACTERIZATION OF NICOTINE ACETYLCHOLINE RECEPTOR SUBUNITS IN THE COCKROACHPeriplaneta americanaMUSHROOM BODIES REVEALS A STRONG EXPRESSION OF β1 SUBUNIT: INVOLVEMENT IN NICOTINE-INDUCED CURRENTS
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Steeve H. Thany and Emiliane Taillebois
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0301 basic medicine ,medicine.medical_specialty ,animal structures ,Physiology ,Biology ,Biochemistry ,Nicotine ,03 medical and health sciences ,0302 clinical medicine ,Ganglion type nicotinic receptor ,Internal medicine ,medicine ,Acetylcholine receptor ,General Medicine ,3. Good health ,Cell biology ,Nicotinic acetylcholine receptor ,030104 developmental biology ,Endocrinology ,Nicotinic agonist ,nervous system ,Insect Science ,Mushroom bodies ,Alpha-4 beta-2 nicotinic receptor ,030217 neurology & neurosurgery ,Cys-loop receptors ,medicine.drug - Abstract
Nicotinic acetylcholine receptors are ligand-gated ion channels expressed in many insect structures, such as mushroom bodies, in which they play a central role. We have recently demonstrated using electrophysiological recordings that different native nicotinic receptors are expressed in cockroach mushroom bodies Kenyon cells. In the present study, we demonstrated that eight genes coding for cockroach nicotinic acetylcholine receptor subunits are expressed in the mushroom bodies. Quantitative real-time polymerase chain reaction (PCR) experiments demonstrated that β1 subunit was the most expressed in the mushroom bodies. Moreover, antisense oligonucleotides performed against β1 subunit revealed that inhibition of β1 expression strongly decreases nicotine-induced currents amplitudes. Moreover, co-application with 0.5 μM α-bungarotoxin completely inhibited nicotine currents whereas 10 μM d-tubocurarine had a partial effect demonstrating that β1-containing neuronal nicotinic acetylcholine receptor subtypes could be sensitive to the nicotinic acetylcholine receptor antagonist α-bungarotoxin.
- Published
- 2016
23. Synergic effect of a quinuclidine benzamide complexed with borane, the LMA10233, in combination with seven pesticides
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Steeve H. Thany, Jacques Lebreton, Emiliane Taillebois, Jérôme Graton, Alison Cartereau, Monique Mathé-Allainmat, Jean-Yves Le Questel, Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), Université d'Orléans (UO)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Chimie Et Interdisciplinarité : Synthèse, Analyse, Modélisation (CEISAM), Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), and Université de Nantes (UN)-Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)
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0106 biological sciences ,0301 basic medicine ,Insecticides ,Quinuclidines ,[SDV]Life Sciences [q-bio] ,Health, Toxicology and Mutagenesis ,01 natural sciences ,Medicinal chemistry ,Acetamiprid ,Neonicotinoids ,03 medical and health sciences ,chemistry.chemical_compound ,Imidacloprid ,Animals ,[CHIM]Chemical Sciences ,Pesticides ,Boranes ,Benzamide ,ComputingMilieux_MISCELLANEOUS ,Fipronil ,Clothianidin ,General Medicine ,Nitro Compounds ,3. Good health ,010602 entomology ,030104 developmental biology ,chemistry ,Chlorpyrifos ,Benzamides ,[SDE]Environmental Sciences ,Toxicity ,Agronomy and Crop Science ,Quinuclidine - Abstract
Some quinuclidine benzamide compounds have been found to modulate nicotinic acetylcholine receptors in both mammals and insects. In particular, the quaternarization of 3-amino quinuclidine benzamide derivatives with dichloromethane gave charged N-chloromethylated quinuclidine compounds, disclosing an antagonist profile on homomeric α7 nAChRs. Here, we synthesized and studied the toxicological effect of LMA10233, a quinuclidine-borane complex analogue, the LMA10233, on the pea aphid Acyrthosiphon pisum and found that LMA10233 only exhibit proper toxicity on A. pisum larvae when applied in concentrations of over 10 μg/ml. We assessed the ability of LMA10233 to enhance the toxicity of different insecticides. When a sublethal concentration of LMA10233 was combined with the LC10 of each compound, we found a strong increase in toxicity at 24 h and 48 h of exposure for clothianidin, fipronil and chlorpyrifos, and only at 24 h for imidacloprid, acetamiprid and deltamethrin. However, when the pesticide was used at the LC50, only acetamiprid showed a synergistic effect with LMA10233. When the concentration of LMA10233 was decreased, we found that up to 80–90% of mortality was obtained due to the synergism between acetamiprid and LMA10233. No similar effect was observed with other insecticides. We conclude that such quinuclidine-borane complex compounds could increase the toxic effect of insecticides at low concentrations.
- Published
- 2020
24. Molecular features and toxicological properties of four common pesticides, acetamiprid, deltamethrin, chlorpyriphos and fipronil
- Author
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Steeve H. Thany, Christine Brazier, Adèle D. Laurent, Emiliane Taillebois, Jean-Yves Le Questel, Zakaria Alamiddine, Jérôme Graton, Laboratoire Récepteurs et Canaux Ioniques Membranaires (RCIM), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA), Chimie Theorique et Modèles (CTOM), Institut des Sciences Moléculaires de Marseille (ISM2), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS), Chimie Et Interdisciplinarité : Synthèse, Analyse, Modélisation (CEISAM), Université de Nantes - Faculté des Sciences et des Techniques, Université de Nantes (UN)-Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS), Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Laboratoire R ecepteurs et Canaux Ioniques Membranaires (RCIM), UPRES EA 2647/USC INRA 1330/SFR 4207 QUASAV, UFR Sciences, Université d'Angers (UA), Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), Université d'Orléans (UO)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-École Centrale de Marseille (ECM)-Institut de Chimie du CNRS (INC)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-École Centrale de Marseille (ECM)-Institut de Chimie du CNRS (INC), Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Institut National de la Recherche Agronomique (INRA)-Université d'Orléans (UO), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and Université de Nantes (UN)-Université de Nantes (UN)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
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Quantum chemistry calculations ,Insecticides ,Insecta ,Pyridines ,Myzus persicae ,Clinical Biochemistry ,Pharmaceutical Science ,010402 general chemistry ,01 natural sciences ,Biochemistry ,Acetamiprid ,Toxicology ,Neonicotinoids ,chemistry.chemical_compound ,Toxicological effect ,Nitriles ,Pyrethrins ,parasitic diseases ,Drug Discovery ,Animals ,Pesticides ,Conformational features ,Aphid ,Molecular Biology ,Fipronil ,Pyrethroid ,Dose-Response Relationship, Drug ,biology ,010405 organic chemistry ,Organic Chemistry ,Acyrthosiphon pisum ,Neonicotinoid ,Molecular electrostatic potential ,Pesticide ,biology.organism_classification ,0104 chemical sciences ,[CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry ,Deltamethrin ,chemistry ,Chlorpyrifos ,Pyrazoles ,Molecular Medicine ,Female - Abstract
International audience; Structural features and selected physicochemical properties of four common pesticides: acetamiprid (neonicotinoid), chlorpyriphos (organophosphate insecticide), deltamethrin (pyrethroid) and fipronil (phenylpyrazole) have been investigated by Density Functional Theory quantum chemical calculations. The high flexible character of these insecticides is revealed by the numerous conformers obtained, located within a 20 kJ mol(-1) range in the gas phase. In line with this trend, a redistribution of the energetic minima is observed in water medium. Molecular electrostatic potential calculations provide a ranking of the potential interaction sites of the four insecticides. The theoretical studies reported in the present work are completed by comparative toxicological assays against three aphid strains. Thus, the same toxicity order for the two susceptible strains Myzus persicae 4106A and Acyrthosiphon pisum LSR1: acetamiprid > fipronil > deltamethrin > chlorpyriphos is revealed. In the resistant strain M. persicae 1300145, the toxicity order is modified: acetamiprid > fipronil > chlorpyriphos > deltamethrin. Interestingly, the strain 1300145 which is known to be resistant to neonicotinoids, is also less sensitive to deltamethrin, chlorpyriphos and fipronil. (C) 2015 Elsevier Ltd. All rights reserved.
- Published
- 2015
25. Ca2+/CALMODULIN-DEPENDENT PROTEIN KINASE II IN THE COCKROACHPeriplaneta americana: IDENTIFICATION OF FIVE ISOFORMS AND THEIR TISSUES DISTRIBUTION
- Author
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Christian Legros, Céline Bourdin, Steeve H. Thany, Sophie Quinchard, Emiliane Taillebois, Hélène Tricoire-Leignel, Audrey Griveau, and Emilie Heuland
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Cloning ,Gene isoform ,0303 health sciences ,Cockroach ,biology ,Physiology ,Kinase ,Alternative splicing ,General Medicine ,biology.organism_classification ,Biochemistry ,Cell biology ,03 medical and health sciences ,0302 clinical medicine ,Insect Science ,biology.animal ,Ca2+/calmodulin-dependent protein kinase ,Botany ,Drosophila melanogaster ,030217 neurology & neurosurgery ,030304 developmental biology ,Periplaneta - Abstract
Calcium/calmodulin-dependent protein kinase II (CaMKII) is a key kinase that transduces Ca²⁺ signals into downstream effects acting on a range of cellular processes in nervous system and muscular tissues. In insects, different CaMKII isoforms have been reported in Drosophila melanogaster, Apis florae, Bombus terrestris, and Bombus impatiens but little is known on the organization and tissue-specific expression of these isoforms with the exception of Drosophila. The present study reports the cloning of five CaMKII splice variants issued from a single gene and their tissue-specific expression in the cockroach Periplaneta americana. Each CaMKII isoform shared 82-90% identity with Drosophila CaMKII isoforms and accordingly were named PaCaMKII-A, PaCaMKII-B,PaCaMKII-C,PaCaMKII-D, and PaCaMKII-E. PaCaMKII-A and PaCaMKII-D isoforms are ubiquitously expressed in all tissues, but some such as PaCaMKII-B andPaCaMKII-C are preferentially expressed in the nerve cord and muscle. In addition, using single-cell reverse transcriptase-polymerase chain reaction (RT-PCR), we found a tissue-specific expression of PaCaMKII-E in the dorsal unpaired median neurons. Alternative splicing of PaCaMKII transcripts is likely a common mechanism in insects to control the pattern of isoform expression in the different tissues.
- Published
- 2013
26. CHARACTERIZATION OF NICOTINE ACETYLCHOLINE RECEPTOR SUBUNITS IN THE COCKROACH Periplaneta americana MUSHROOM BODIES REVEALS A STRONG EXPRESSION OF β1 SUBUNIT: INVOLVEMENT IN NICOTINE-INDUCED CURRENTS
- Author
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Emiliane, Taillebois and Steeve H, Thany
- Subjects
Male ,Gene Expression ,Receptors, Nicotinic ,Bungarotoxins ,Real-Time Polymerase Chain Reaction ,Gene Expression Regulation ,Animals ,Insect Proteins ,Periplaneta ,Amino Acid Sequence ,RNA, Messenger ,Cloning, Molecular ,Sequence Alignment ,Mushroom Bodies ,Phylogeny - Abstract
Nicotinic acetylcholine receptors are ligand-gated ion channels expressed in many insect structures, such as mushroom bodies, in which they play a central role. We have recently demonstrated using electrophysiological recordings that different native nicotinic receptors are expressed in cockroach mushroom bodies Kenyon cells. In the present study, we demonstrated that eight genes coding for cockroach nicotinic acetylcholine receptor subunits are expressed in the mushroom bodies. Quantitative real-time polymerase chain reaction (PCR) experiments demonstrated that β1 subunit was the most expressed in the mushroom bodies. Moreover, antisense oligonucleotides performed against β1 subunit revealed that inhibition of β1 expression strongly decreases nicotine-induced currents amplitudes. Moreover, co-application with 0.5 μM α-bungarotoxin completely inhibited nicotine currents whereas 10 μM d-tubocurarine had a partial effect demonstrating that β1-containing neuronal nicotinic acetylcholine receptor subtypes could be sensitive to the nicotinic acetylcholine receptor antagonist α-bungarotoxin.
- Published
- 2016
27. Synthesis and biological activity of fluorescent neonicotinoid insecticide thiamethoxam
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Emiliane Taillebois, Denis Séraphin, Steeve H. Thany, Paul Langlois, Thomas Cunha, Signalisation Fonctionnelle des Canaux Ioniques et Récepteurs (SIFCIR), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA), Substances d'Origine Naturelle et Analogues Structuraux (SONAS), and Université d'Angers (UA)
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Insecticides ,[SDV]Life Sciences [q-bio] ,Clinical Biochemistry ,Molecular Conformation ,Pharmaceutical Science ,Receptors, Nicotinic ,Biochemistry ,Neonicotinoids ,Structure-Activity Relationship ,chemistry.chemical_compound ,biology.animal ,Oxazines ,Drug Discovery ,Botany ,Animals ,Mode of action ,Molecular Biology ,Fluorescent Dyes ,Acetylcholine receptor ,Cockroach ,Dose-Response Relationship, Drug ,biology ,Organic Chemistry ,Neonicotinoid ,neonicotinoid ,insecticide ,Biological activity ,Nitro Compounds ,biology.organism_classification ,3. Good health ,Acyrthosiphon pisum ,Thiazoles ,Nicotinic agonist ,chemistry ,thiametoxam ,Aphids ,Molecular Medicine ,insect ,Thiamethoxam ,nicotinic receptor - Abstract
International audience; Here, we describe the synthesis of two new fluorescent derivatives of thiamethoxam and compared their toxicity on aphid Acyrthosiphon pisum and their mode of action on insect nicotinic acetylcholine receptors expressed on the sixth abdominal ganglion. The compound 3 with two 2-chlorothiazole moieties was found to be more toxic using toxicological bioassays 24 h and 48 h after exposure while compound 4 appeared more active using cockroach ganglionic depolarization. Interestingly, thiamethoxam appeared more effective than component 3 and 4, respectively. Our results demonstrated that component 3 and 4 act as agonists of insect nicotinic acetylcholine receptors
- Published
- 2014
28. Inhibition of PaCaMKII-E isoform in the dorsal unpaired median neurosecretory cells of cockroach reduces nicotine- and clothianidin-induced currents
- Author
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Delphine Calas-List, Emilie Heuland, Marjorie Juchaux, Olivier List, Emiliane Taillebois, Steeve H. Thany, Laboratoire R ecepteurs et Canaux Ioniques Membranaires (RCIM), UPRES EA 2647/USC INRA 1330/SFR 4207 QUASAV, UFR Sciences, Université d'Angers (UA), Institut de Recherche en Horticulture et Semences (IRHS), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, AGROCAMPUS OUEST-Institut National de la Recherche Agronomique (INRA)-Université d'Angers (UA), Récepteurs et Canaux Ioniques Membranaires (RCIM), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), AGROCAMPUS OUEST, and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de la Recherche Agronomique (INRA)-Université d'Angers (UA)
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Male ,Patch-Clamp Techniques ,Cockroaches ,Receptors, Nicotinic ,Biochemistry ,Guanidines ,Calcium in biology ,chemistry.chemical_compound ,Neonicotinoids ,Nicotinic Agonists ,CaMKII ,acetylcholine-receptors ,periplaneta-americana ,escape behavior ,drosophila ,Immunohistochemistry ,Cell biology ,Nicotinic acetylcholine receptor ,Nicotinic agonist ,Gene isoform ,medicine.medical_specialty ,nicotinic receptors ,dum neurons ,chemistry.chemical_element ,Biology ,Calcium ,Real-Time Polymerase Chain Reaction ,clothianidin ,Cellular and Molecular Neuroscience ,developmental-changes ,Ca2+/calmodulin-dependent protein kinase ,Internal medicine ,medicine ,protein-kinase-ii ,Animals ,Calcium Signaling ,Acetylcholine receptor ,calcium ,electrical-activity ,[SCCO.NEUR]Cognitive science/Neuroscience ,Clothianidin ,Neurosecretory Systems ,Electrophysiological Phenomena ,Ganglia, Invertebrate ,Thiazoles ,Endocrinology ,chemistry ,activation ,Calcium Channels ,Calcium-Calmodulin-Dependent Protein Kinase Type 2 ,nicotine - Abstract
Cellular responses to Ca2+ require intermediary proteins such as calcium/calmodulin-dependent protein kinase II (CaMKII), which transduces the signal into downstream effects. We recently demonstrated that the cockroach genome encodes five different CaMKII isoforms, and only PaCaMKII-E isoform is specifically expressed in the dorsal unpaired median neurosecretory cells. In the present study, using antisense oligonucleotides, we demonstrated that PaCaMKII-E isoform inhibition reduced nicotine-induced currents through α-bungarotoxin-sensitive and -insensitive nicotinic acetylcholine receptor subtypes. Specifically, PaCaMKII-E isoform is sufficient to repress nicotinic current amplitudes as a result of its depression by antisense oligonucleotides. Similar results were found using the neonicotinoid insecticide clothianidin, which acted as a full agonist of dorsal unpaired median neuron nicotinic acetylcholine receptors. Clothianidin current amplitudes are strongly reduced under bath application of PaCaMKII-E antisense oligonucleotides but no significant results are found with α-bungarotoxin co-applied, demonstrating that CaMKII-E isoform affects nicotine currents through α-bungarotoxin-sensitive and -insensitive receptor subtypes whereas clothianidin currents are reduced via α-bungarotoxin-insensitive receptors. In addition, we found that intracellular calcium increase induced by nicotine and clothianidin were reduced by PaCaMKII-E antisense oligonucleotides, demonstrating that intracellular calcium increase induced by nicotine and clothianidin are affected by PaCaMKII-E inhibition. Cellular responses to Ca2+ require intermediary proteins such as calcium/calmodulin-dependent protein kinase II (CaMKII). We recently demonstrated that the cockroach genome encodes five different CaMKII isoforms and only PaCaMKII-E isoform was specifically expressed in the dorsal unpaired median neurosecretory cells. Here we show that specific inhibition of PaCaMKII-E isoform is associated with a decrease in nicotine- and clothianidin-induced currents. In addition, analysis of calcium changes demonstrates that PaCaMKII-E inhibition induces a decrease in intracellular calcium concentration.
- Published
- 2014
29. Neonicotinoid binding, toxicity and expression of nicotinic acetylcholine receptor subunits in the aphid Acyrthosiphon pisum
- Author
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Steeve H. Thany, Antoine Daguin, Abdelhamid Beloula, Stéphanie Jaubert-Possamai, Sophie Quinchard, Hélène Tricoire-Leignel, Emiliane Taillebois, Denis Servent, Denis Tagu, Laboratoire Récepteurs et Canaux Ioniques Membranaires (RCIM), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA), Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, GIRPA, Groupement Interprofessionnel de Recherche sur les Produits Agropharmaceutiques, Service d'Ingénierie Moléculaire pour la Santé (ex SIMOPRO) (SIMoS), Médicaments et Technologies pour la Santé (MTS), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), INRA Département SPE, Ministère de la Recherche et de l'Enseignement Supérieur, Récepteurs et Canaux Ioniques Membranaires (RCIM), Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST, Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), AGROCAMPUS OUEST-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Recherche Agronomique (INRA), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)
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Insecticides ,Nicotinic Acetylcholine Receptors ,[SDV]Life Sciences [q-bio] ,lcsh:Medicine ,Nicotinic Antagonists ,Plant Science ,Receptors, Nicotinic ,Toxicology ,Guanidines ,Biochemistry ,chemistry.chemical_compound ,Neonicotinoids ,Tandem Mass Spectrometry ,Nicotinic Antagonist ,lcsh:Science ,Chromatography, High Pressure Liquid ,Aphid ,Plant Pests ,Multidisciplinary ,biology ,Imidazoles ,food and beverages ,Agriculture ,Nitro Compounds ,Nicotinic acetylcholine receptor ,Larva ,Thiamethoxam ,Agrochemicals ,Protein Binding ,Research Article ,Transmembrane Receptors ,récepteur nicotinique acétylcholine ,acyrthosiphon pisum ,Imidacloprid ,Oxazines ,Animals ,Pesticides ,Binding Sites ,lcsh:R ,Cell Membrane ,insecticide ,Neonicotinoid ,Clothianidin ,Biology and Life Sciences ,Proteins ,insecta ,imidacloprid ,Plant Pathology ,biology.organism_classification ,Bungarotoxins ,Molecular biology ,Acyrthosiphon pisum ,Protein Subunits ,Thiazoles ,chemistry ,Aphids ,Acetylcholine Receptors ,lcsh:Q ,Pest Control ,Zoology ,Entomology - Abstract
International audience; Neonicotinoid insecticides act on nicotinic acetylcholine receptor and are particularly effective against sucking pests. They are widely used in crops protection to fight against aphids, which cause severe damage. In the present study we evaluated the susceptibility of the pea aphid Acyrthosiphon pisum to the commonly used neonicotinoid insecticides imidacloprid (IMI), thiamethoxam (TMX) and clothianidin (CLT). Binding studies on aphid membrane preparations revealed the existence of high and low-affinity binding sites for [H-3]-IMI (Kd of 0.16 +/- 0.04 nM and 41.7 +/- 5.9 nM) and for the nicotinic antagonist [I-125]-alpha- bungarotoxin (Kd of 0.008 +/- 0.002 nM and 1.135 +/- 0.213 nM). Competitive binding experiments demonstrated that TMX displayed a higher affinity than IMI for [I-125]-alpha-bungarotoxin binding sites while CLT affinity was similar for both [I-125]-alpha-bungarotoxin and [H-3]-IMI binding sites. Interestingly, toxicological studies revealed that at 48 h, IMI (LC50 = 0.038 mu g/ml) and TMX (LC50 = 0.034 mu g/ml) were more toxic than CLT (LC50 = 0.118 mu g/ml). The effect of TMX could be associated to its metabolite CLT as demonstrated by HPLC/MS analysis. In addition, we found that aphid larvae treated either with IMI, TMX or CLT showed a strong variation of nAChR subunit expression. Using semi-quantitative PCR experiments, we detected for all insecticides an increase of Apisum alpha 10 and Apisum beta 1 expressions levels, whereas Apisum beta 2 expression decreased. Moreover, some other receptor subunits seemed to be differently regulated according to the insecticide used. Finally, we also demonstrated that nAChR subunit expression differed during pea aphid development. Altogether these results highlight species specificity that should be taken into account in pest management strategies
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- 2013
30. Ca²⁺/calmodulin-dependent protein kinase II in the cockroach Periplaneta americana: identification of five isoforms and their tissues distribution
- Author
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Emiliane, Taillebois, Emilie, Heuland, Céline M, Bourdin, Audrey, Griveau, Sophie, Quinchard, Helene, Tricoire-Leignel, Christian, Legros, and Steeve H, Thany
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Alternative Splicing ,Gene Expression Regulation ,Reverse Transcriptase Polymerase Chain Reaction ,Animals ,Computational Biology ,Periplaneta ,Protein Isoforms ,Tissue Distribution ,Cloning, Molecular ,Calcium-Calmodulin-Dependent Protein Kinase Type 2 ,DNA Primers - Abstract
Calcium/calmodulin-dependent protein kinase II (CaMKII) is a key kinase that transduces Ca²⁺ signals into downstream effects acting on a range of cellular processes in nervous system and muscular tissues. In insects, different CaMKII isoforms have been reported in Drosophila melanogaster, Apis florae, Bombus terrestris, and Bombus impatiens but little is known on the organization and tissue-specific expression of these isoforms with the exception of Drosophila. The present study reports the cloning of five CaMKII splice variants issued from a single gene and their tissue-specific expression in the cockroach Periplaneta americana. Each CaMKII isoform shared 82-90% identity with Drosophila CaMKII isoforms and accordingly were named PaCaMKII-A, PaCaMKII-B,PaCaMKII-C,PaCaMKII-D, and PaCaMKII-E. PaCaMKII-A and PaCaMKII-D isoforms are ubiquitously expressed in all tissues, but some such as PaCaMKII-B andPaCaMKII-C are preferentially expressed in the nerve cord and muscle. In addition, using single-cell reverse transcriptase-polymerase chain reaction (RT-PCR), we found a tissue-specific expression of PaCaMKII-E in the dorsal unpaired median neurons. Alternative splicing of PaCaMKII transcripts is likely a common mechanism in insects to control the pattern of isoform expression in the different tissues.
- Published
- 2013
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