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1. Survival and infectivity of Toxoplasma gondii and Cryptosporidium parvum oocysts bioaccumulated by Dreissena polymorpha

Author

Stéphane Betoulle, Aurélie Bigot-Clivot, S. La Carbona, Gilles Gargala, Angélique Rousseau, Sandie Escotte-Binet, Loïc Favennec, Elodie Géba, Isabelle Villena, Dominique Aubert, Jitender P. Dubey, A. Le Guernic, Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Institut National de l'Environnement Industriel et des Risques (INERIS), Epidémiosurveillance de protozooses à transmission alimentaire et vectorielle (ESCAPE), Université de Reims Champagne-Ardenne (URCA)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), ACTALIA [Villers-Bocage], USDA Agricultural Research Service [Beltsville, Maryland], USDA-ARS : Agricultural Research Service, and Centre Hospitalier Universitaire de Reims (CHU Reims)

Subjects
Applied Microbiology and Biotechnology, Dreissena, Microbiology, 03 medical and health sciences, Animals, Bioassay, ComputingMilieux_MISCELLANEOUS, Feces, 030304 developmental biology, Cryptosporidium parvum, Infectivity, 0303 health sciences, biology, 030306 microbiology, Oocysts, Water, Toxoplasma gondii, General Medicine, Mussel, biology.organism_classification, 6. Clean water, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Biodegradation, Environmental, [SDE]Environmental Sciences, Zebra mussel, Toxoplasma, Biotechnology
Abstract

Aims The study was aimed to understand the depuration process of Cryptosporidium parvum and Toxoplasma gondii oocysts by zebra mussel (Dreissena polymorpha), to consider the use of the zebra mussel as a bioremediation tool. Materials and methods Two experiments were performed: (i) individual exposure of mussel to investigate oocyst transfers between bivalves and water and (ii) in vivo exposure to assess the ability of the zebra mussel to degrade oocysts. Results (i) Our results highlighted a transfer of oocysts from the mussels to the water after 3 and 7 days of depuration; however, some oocysts were still bioaccumulated in mussel tissue. (ii) Between 7 days of exposure at 1000 or 10 000 oocysts/mussel/day and 7 days of depuration, the number of bioaccumulated oocysts did not vary but the number of infectious oocysts decreased. Conclusion Results show that D. polymorpha can release oocysts in water via (pseudo)faeces in depuration period. Oocysts remain bioaccumulated and infectious oocyst number decreases during the depuration period in zebra mussel tissues. Results suggest a degradation of bioaccumulated C. parvum and T. gondii oocysts. Significance and impact of the study This study highlighted the potential use of D. polymorpha as a bioremediation tool to mitigate of protozoan contamination in water resources.

Published
2020

2. Blue mussel (Mytilus edulis)-A bioindicator of marine water contamination by protozoa: Laboratory and in situ approaches

Author

Elodie Géba, Benoit Xuereb, Isabelle Bonnard, Dominique Aubert, Houssem Chalghmi, Isabelle Villena, Catherine Cazeaux, Aurélie Bigot-Clivot, Alain Geffard, Jitender P. Dubey, Stéphanie La Carbona, Fanny Bastien, Loïc Durand, Sandie Escotte-Binet, Frank Le Foll, Mélissa Palos Ladeiro, Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), Institut National de l'Environnement Industriel et des Risques (INERIS)-Université de Reims Champagne-Ardenne (URCA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), ACTALIA [Villers-Bocage], Epidémiosurveillance de protozooses à transmission alimentaire et vectorielle (ESCAPE), Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Université de Reims Champagne-Ardenne (URCA), Animal Parasitic Diseases Laboratory [Beltsville, USA], United States Department of Agriculture (USDA)-USDA Agricultural Research Service [Beltsville, Maryland], USDA-ARS : Agricultural Research Service-USDA-ARS : Agricultural Research Service, and Centre Hospitalier Universitaire de Reims (CHU Reims)

Subjects
animal structures, Mytilus edulis, Zoology, Toxoplasma gondii, Cryptosporidiosis, Cryptosporidium, Applied Microbiology and Biotechnology, bivalve, marine water, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, parasitic diseases, Animals, 14. Life underwater, Ecosystem, Cryptosporidium parvum, [SDV.EE.SANT]Life Sciences [q-bio]/Ecology, environment/Health, biology, Environmental Biomarkers, fungi, Water, General Medicine, Mussel, Contamination, biology.organism_classification, Mytilus, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, 13. Climate action, Bioaccumulation, biomonitoring, Protozoa, Laboratories, Bioindicator, Blue mussel, Biotechnology, Giardia duodenalis
Abstract

International audience; Aims: The protozoan parasites Cryptosporidium spp., Giardia duodenalis and Toxoplasma gondii are identified as public health priorities and are present in a wide variety of environments including the marine ecosystem. The objective of this study was to demonstrate that the marine bivalve blue mussel (Mytilus edulis) can be used as a tool to monitor the contamination of marine waters by the three protozoa over time. Methods and Results: In order to achieve a proof of concept, mussels were exposed to three concentrations of G. duodenalis cysts and Cryptosporidium parvum/T. gondii oocysts for 21 days, followed by 21 days of depuration in clear water. Then, natural contamination by these protozoa was sought for in wild marine blue mussels along the northwest coast of France to validate their relevance as bioindicators in the field. Our results highlighted that: (a) blue mussels bioaccumulated the parasites for 21 days, according to the conditions of exposure, and parasites could still be detected during the depuration period (until 21 days); (b) the percentage of protozoa-positive M. edulis varied under the degree of protozoan contamination in water; (c) mussel samples from eight out of nine in situ sites were positive for at least one of the protozoa. Conclusions: The blue mussel M. edulis can bioaccumulate protozoan parasites over long time periods, according to the degree of contamination of waters they are inhabiting, and can highlight recent but also past contaminations (at least 21 days). Significance and Impact of the Study: Mytilus edulis is a relevant bioaccumulators of protozoan (oo)cysts in laboratory and field conditions, hence its potential use for monitoring parasite contamination in marine waters.

Published
2021

3. Resilience of Dreissena polymorpha in wastewater effluent: Use as a bioremediation tool?

Author

Stéphane Betoulle, Damien Rioult, Dominique Aubert, Aurélie Bigot-Clivot, Odile Dedourge-Geffard, Olivier Palluel, Elodie Géba, Université de Reims Champagne-Ardenne (URCA), Plateau Technique de Cytométrie en Flux (URCACyt), and Institut National de l'Environnement Industriel et des Risques (INERIS)

Subjects
animal structures, Environmental Engineering, 0208 environmental biotechnology, Context (language use), 02 engineering and technology, Wastewater, 010501 environmental sciences, Management, Monitoring, Policy and Law, 01 natural sciences, Dreissena, Bioremediation, Animals, 14. Life underwater, Waste Management and Disposal, Effluent, ComputingMilieux_MISCELLANEOUS, 0105 earth and related environmental sciences, biology, General Medicine, biology.organism_classification, Pulp and paper industry, 6. Clean water, Bivalvia, 020801 environmental engineering, Biodegradation, Environmental, Biofilter, [SDE]Environmental Sciences, Zebra mussel, Sewage treatment, Toxoplasma
Abstract

Nowadays, it is necessary to improve the efficiency of wastewater treatment plant treatments. In this context the use of biofilter species, like Dreissena polymorpha, as a bioremediation tool in wastewater is increasingly highlighted. The innovative aim of this study is to evaluate the zebra mussel survival in the outlet channel of a conventional WWTP to use them as bioremediation tool. For this, mussels were transplanted in the outlet channel for 28 days and different biomarkers were monitored. D. polymorpha is able to maintain itself in good physiological conditions until 21 days, yet at 28 days a high mortality rate (24%), a decrease in filtration efficiency (8/15 mussels filtered and 17.0% of filtration rate) and antioxidant system activation (CAT activity et gpx gene expression increase) suggest an exhaustion. Some biomarkers suggested a hypoxic stress. Despite the unfavourable conditions, bivalves have bioaccumulated pathogenic protozoa (Toxoplasma gondii and Giardia duodenalis) during the exposure. Zebra mussel seems to be a promising tool for bioremediation in wastewater.

Published
2021

4. Use of the bivalve Dreissena polymorpha as a biomonitoring tool to reflect the protozoan load in freshwater bodies

Author

Dominique Aubert, Elodie Géba, Catherine Cazeaux, Jitender P. Dubey, Alain Geffard, Fanny Bastien, Stéphanie La Carbona, Mélissa Palos Ladeiro, Isabelle Villena, Loïc Durand, Sandy Escotte, Aurélie Bigot-Clivot, Isabelle Bonnard, Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), Institut National de l'Environnement Industriel et des Risques (INERIS)-Université de Reims Champagne-Ardenne (URCA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Epidémiosurveillance de protozooses à transmission alimentaire et vectorielle (ESCAPE), Université de Reims Champagne-Ardenne (URCA)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Actalia [Saint-Lô], Animal Parasitic Diseases Laboratory [Beltsville, USA], United States Department of Agriculture (USDA)-USDA Agricultural Research Service [Beltsville, Maryland], and USDA-ARS : Agricultural Research Service-USDA-ARS : Agricultural Research Service

Subjects
animal structures, Environmental Engineering, Freshwater bivalve, animal diseases, 0208 environmental biotechnology, Cryptosporidiosis, Cryptosporidium, Zoology, Fresh Water, 02 engineering and technology, 010501 environmental sciences, 01 natural sciences, Dreissena, parasitic diseases, Biomonitoring, Animals, 14. Life underwater, Waste Management and Disposal, Ecosystem, ComputingMilieux_MISCELLANEOUS, 0105 earth and related environmental sciences, Water Science and Technology, Civil and Structural Engineering, [SDV.EE]Life Sciences [q-bio]/Ecology, environment, biology, Ecological Modeling, Aquatic ecosystem, fungi, Mussel, biology.organism_classification, Pollution, 6. Clean water, Bivalvia, 020801 environmental engineering, Cryptosporidium parvum, Zebra mussel, Protozoa, Biological Monitoring
Abstract

Cryptosporidium parvum, Toxoplasma gondii and Giardia duodenalis are worldwide pathogenic protozoa recognized as major causal agents of waterborne disease outbreaks. To overcome the normative process (ISO 15553/2006) limitations of protozoa detection in aquatic systems, we propose to use the zebra mussel (Dreissena polymorpha), a freshwater bivalve mollusc, as a tool for biomonitoring protozoan contamination. Mussels were exposed to three concentrations of C. parvum oocysts, G. duodenalis cysts or T. gondii oocysts for 21 days followed by 21 days of depuration in clear water. D. polymorpha accumulated protozoa in its tissues and haemolymph. Concerning T. gondii and G. duodenalis, the percentage of protozoa positive mussels reflected the contamination level in water bodies. As for C. parvum detection, oocysts did accumulate in mussel tissues and haemolymph, but in small quantities, and the limit of detection was high (between 50 and 100 oocysts). Low levels of T. gondii (1–5 oocysts/mussel) and G. duodenalis (less than 1 cyst/mussel) were quantified in D. polymorpha tissues. The ability of zebra mussels to reflect contamination by the three protozoa for weeks after the contamination event makes them a good integrative matrix for the biomonitoring of aquatic ecosystems.

Published
2020

5. Mussel as a Tool to Define Continental Watershed Quality

Author

Maxime Lepretre, Gabrielle Magniez, Iris Barjhoux, Marc Bonnard, Damien Rioult, Gaëlle Daniele, Mélissa Palos-Ladeiro, Dominique Aubert, Emilie Lance, Odile Dedourge-Geffard, Elise David, Aurélie Bigot-Clivot, Elodie Géba, Arnaud Salvador, Jean Armengaud, Alain Geffard, Emmanuelle Vulliet, Isabelle Villena, Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Institut National de l'Environnement Industriel et des Risques (INERIS), Protozooses Transmises par l'Alimentation (Cryptosporidiose, Giardose et Toxoplasmose) : Mode de Contamination et Pathogénie (PROTAL) - EA 3800 (PROTAL), Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA), Institut des Sciences Analytiques (ISA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), TRACES - Technologie et Recherche en Analyse Chimique pour l'Environnement et la Santé, Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), ANABIO-MS - Analyse biomoléculaire par spectrométrie de masse - Biological Analysis by Mass Spectrometry, Commissariat à l'Energie Atomique, Centre de Marcoule (CEA), Institut National de l'Environnement Industriel et des Risques (INERIS)-Université de Reims Champagne-Ardenne (URCA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), Epidémiosurveillance de protozooses à transmission alimentaire et vectorielle (ESCAPE), Université de Reims Champagne-Ardenne (URCA)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Centre de formation et de recherche sur l'environnement marin (CEFREM), Université de Perpignan Via Domitia (UPVD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), University of Reims Champagne-Ardenne, Fluides, automatique, systèmes thermiques (FAST), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biochimie des Systèmes Perturbés (LBSP), Unité de Recherche Interactions Animal-Environnement - EA 4689 (IAE), Université de Reims Champagne-Ardenne (URCA), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), and Normandie Université (NU)-Normandie Université (NU)-Université de Reims Champagne-Ardenne (URCA)

Subjects
0301 basic medicine, [SDV.EE]Life Sciences [q-bio]/Ecology, environment, Watershed, media_common.quotation_subject, [SDV]Life Sciences [q-bio], Mussel, 010501 environmental sciences, 01 natural sciences, Fishery, 03 medical and health sciences, 030104 developmental biology, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, [SDE]Environmental Sciences, Environmental science, [CHIM]Chemical Sciences, Quality (business), [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology, ComputingMilieux_MISCELLANEOUS, 0105 earth and related environmental sciences, media_common
Abstract

International audience

Published
2017

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