10 results on '"Elmongy, H."'
Search Results
2. First Clinical Report of Pencil Beam Scanned Proton Therapy for Mediastinal Lymphoma
- Author
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Plastaras, J.P., primary, Vogel, J., additional, Elmongy, H., additional, Zeng, C., additional, Svoboda, J., additional, Both, S., additional, and Maity, A., additional
- Published
- 2016
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3. The Use of Consolidative Proton Therapy After First-Line Therapy Among Patients With Hodgkin Lymphoma at Academic and Community Proton Centers
- Author
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Hoppe, B.S., primary, Hill-Kayser, C.E., additional, Tseng, Y.D., additional, Flampouri, S., additional, Elmongy, H., additional, Cahlon, O., additional, Mendenhall, N.P., additional, Maity, A., additional, McGee, L.A., additional, and Plastaras, J.P., additional
- Published
- 2016
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4. Determination and Pharmacokinetics of Omeprazole Enantiomers in Human Plasma and Oral Fluid Utilizing Microextraction by Packed Sorbent and Liquid Chromatography-Tandem Mass Spectrometry.
- Author
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Ahmed H, Wahbi AA, Elmongy H, Amini A, Koyi H, Branden E, and Abdel-Rehim M
- Abstract
In the present work, the determination of omeprazole (OME) enantiomers in oral fluid and plasma samples was carried out utilizing microextraction by packed sorbent (MEPS) and liquid chromatography-tandem mass spectrometry. A chiral column with cellulose-SB phase was used for the first time for enantiomeric separation of OME with an isocratic elution system using 0.2% ammonium hydroxide in hexane-ethanol mixture (70 : 30, v/v) as the mobile phase. OME enantiomers were determined utilizing a triple quadrupole tandem mass spectrometer in positive ion mode (ESI+) monitoring mass transitions: m / z 346.3 ⟶ 198.0 for OME and m / z 369.98 ⟶ 252.0 for internal standard. The limits of detection and quantification of the present method for both enantiomers were 0.1 and 0.4 ng/mL, respectively. The method validation provided good accuracy and precision. The matrix effect factor was less than 5%, and no interfering peaks were observed. The interday precision values ranged from 2.2 to 7.5 (%RSD), and the accuracy of determinations varied from -9.9% to 8.3%. In addition, the pharmacokinetics (PK) of omeprazole enantiomers in healthy subjects after a single oral dose was investigated. (S)-Enantiomers showed higher levels than (R)-enantiomers throughout 24 h. It was found that the mean maximum concentrations of (R)- and (S)-omeprazole in plasma samples were about two times higher than in oral fluid., Competing Interests: The authors declare that they have no conflicts of interest., (Copyright © 2021 Hytham Ahmed et al.)
- Published
- 2021
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5. Longitudinal studies of putative growth hormone (GH) biomarkers and hematological and steroidal parameters in relation to 2 weeks administration of human recombinant GH.
- Author
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Sieckmann T, Elmongy H, Ericsson M, Bhuiyan H, Lehtihet M, and Ekström L
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- Adult, Athletes, Biomarkers blood, Dihydrotestosterone blood, Doping in Sports methods, Fibronectins blood, Human Growth Hormone blood, Humans, Insulin-Like Growth Factor I analysis, Longitudinal Studies, Male, Recombinant Proteins blood, Steroids blood, Testosterone blood, Vitamin D-Binding Protein blood, Biomarkers analysis, Human Growth Hormone analysis, Recombinant Proteins analysis, Steroids analysis
- Abstract
The detection of low doses of recombinant growth hormone is a challenge in antidoping testing. Future testing may lead toward the longitudinal monitoring of IGF-I and P-III-NP in an endocrine module. Additional biomarkers, for example vitamin D binding protein, alpha-HS-glycoprotein, fibronectin 1, and decorin have been identified in different omics studies. This was a longitudinal study of the usefulness of these putative biomarkers in relation to 2 weeks administration of low doses of recombinant growth hormone in healthy male volunteers. Moreover, the hematological parameters included in the athlete biological passport were studied as well as the serum concentration of testosterone and dihydrotestosterone. Fibronectin 1 increased by 20% during the treatment period (P ˂ 0.05), confirming the previous finding. Alpha-HS-glycoprotein decreased by 25% up to 3 weeks after treatment (P ˂ 0.05), contradicting previous results. The addition of fibronectin 1 increased the likelihood of detecting recombinant growth hormone intake based on individual calculated thresholds in some of the participants compared with the GH2000, IGF-I, and P-III-NP. The multiplication of fibronectin 1 concentration by IGF-I resulted in the most profound (up to 4-fold) changes. A minor 15% increase (P = 0.003) in the reticulocyte percentage was observed, but the changes did not lead to any atypical profile based on individual passport thresholds. Vitamin D binding protein, decorin, testosterone, and dihydrotestosterone were not affected by growth hormone. Dihydrotestosterone sulfate was negatively correlated with IGF-I at baseline (R = -0.50, P = 0.003) and post dose (R = -0.59, P = 0.01). In conclusion, fibronectin 1 was verified as a promising future biomarker for detecting low doses of recombinant growth hormone., (© 2020 John Wiley & Sons, Ltd.)
- Published
- 2020
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6. Development and validation of a UHPLC-HRMS method for the simultaneous determination of the endogenous anabolic androgenic steroids in human serum.
- Author
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Elmongy H, Masquelier M, and Ericsson M
- Subjects
- Anabolic Agents blood, Female, Humans, Male, Solid Phase Extraction, Chromatography, High Pressure Liquid, Doping in Sports methods, Mass Spectrometry, Steroids blood
- Abstract
Being performance enhancing hormones, endogenous anabolic androgenic steroids (EAAS) are banned from most competitive sports by the World Anti-doping Agency (WADA). In anti-doping control laboratories, routine assays are mainly performed on urine samples of athletes in and out of competitions. Serum constitutes a promising alternative to urine as it is less subjected to manipulation or contamination that may influence the method sensitivity. The simultaneous determination of EAAS including conjugated metabolites using LC-MS is very challenging due to their contradicting chemical behaviors at the ionization interface of the mass spectrometer. This may prejudice their detection or limit the method sensitivity. Herein, we have addressed these challenges and developed a new method for the simultaneous determination of unconjugated, sulphate- and glucuronide-conjugated EAAS (Androsterone, Etiocholanolone, testosterone, epitestosterone, dihydrotestosterone, dehydroepiandrosterone, androstenedione and 17a-hydroxyprogesterone) in human serum using ultra-high performance liquid chromatography coupled to high-resolution mass spectrometry (UHPLC-HRMS). The use of mass spectrometric detection in full scan mode facilitated the study of the most versatile adducts for detection and quantitation. A solid phase extraction method was developed for the sample preparation prior to analysis. The method limits of quantitation ranged from 0.006 to 7.904 ng/mL and the recoveries ranged from 70.2% to 96.5%. The method calibration was performed in untreated serum representing realistic matrix composition with correlation coeffecients ranged from 0.9859 to 0.9988. Finally, the serum-levels of the investigated steroids were determined in 4 male and 1 female human subjects to provide estimates of baseline levels based on individual values., Competing Interests: Declaration of Competing Interest None., (Copyright © 2019. Published by Elsevier B.V.)
- Published
- 2020
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7. Nanomaterials as sorbents for sample preparation in bioanalysis: A review.
- Author
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Ahmadi M, Elmongy H, Madrakian T, and Abdel-Rehim M
- Subjects
- Organic Chemicals, Polymers, Solid Phase Extraction, Solid Phase Microextraction, Biological Assay, Nanostructures
- Abstract
In recent years, application of nanomaterials as sorbent has gained the attention of researchers in bioanalysis. Different nanomaterials have been utilized as the sorbent in extraction techniques such as solid phase extraction, dispersive solid phase extraction, magnetic solid phase extraction, microextraction by packed sorbent, solid phase microextraction, dispersive μ-solid phase extraction, and stir bar sorptive extraction. In the present review, different nanomaterials which have recently been utilized as sorbent for bioanalysis are classified into six main groups, namely metallic, metallic and mixed oxide, magnetic, carbonaceous, silicon, and polymer-based nanomaterials. Application of these nanomaterials in different extraction techniques for bioanalysis has been reviewed. This study shows that magnetic nanomaterials have gained significant attention owing to their magnetic separation ability. In addition, the present review shows that there is a lack in the application of nanomaterials for on-line analysis procedures, most probably due to some intrinsic properties of nanomaterials such as spontaneous agglomeration., (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Published
- 2017
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8. Determination of metoprolol enantiomers in human plasma and saliva samples utilizing microextraction by packed sorbent and liquid chromatography-tandem mass spectrometry.
- Author
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Elmongy H, Ahmed H, Wahbi AA, Amini A, Colmsjö A, and Abdel-Rehim M
- Subjects
- Adrenergic beta-Antagonists blood, Humans, Limit of Detection, Metoprolol blood, Reproducibility of Results, Stereoisomerism, Adrenergic beta-Antagonists metabolism, Chromatography, Liquid methods, Metoprolol metabolism, Saliva metabolism, Tandem Mass Spectrometry methods
- Abstract
A sensitive, accurate and reliable bioanalytical method for the enantioselective determination of metoprolol in plasma and saliva samples utilizing liquid chromatography-electrospray ionization tandem mass spectrometry was developed and validated. Human plasma and saliva samples were pretreated by microextraction by packed sorbent (MEPS) prior to analysis. A new MEPS syringe form with two inputs was used. Metoprolol enantiomers and internal standard pentycaine (IS) were eluted from MEPS sorbent using isopropanol after removal of matrix interferences using aliquots of 5% methanol in water. Complete separation of metoprolol enantiomers was achieved on a Cellulose-SB column (150 × 4.6 mm, 5 μm) using isocratic elution with mobile phase 0.1% ammonium hydroxide in hexane-isopropanol (80:20, v/v) with a flow rate of 0.8 mL/min. A post-column solvent-assisted ionization was applied to enhance metoprolol ionization signal in positive mode monitoring (+ES) using 0.5% formic acid in isopropanol at a flow rate of 0.2 mL/min. The total chromatographic run time was 10 min for each injection. The detection of metoprolol in plasma and saliva samples was performed using triple quadrupole tandem mass spectrometer in +ES under the following mass transitions: m/z 268.08 → 72.09 for metoprolol and m/z 303.3 → 154.3 for IS. The linearity range was 2.5-500 ng/mL for both R- and S-metoprolol in plasma and saliva. The limits of detection and quantitation for both enantiomers were 0.5 and 2.5 ng/mL respectively, in both matrices (plasma and saliva). The intra- and inter-day precisions were presented in terms of RSD values for replicate analysis of quality control samples and were <5%; the accuracy of determinations varied from 96 to 99%. The method was able to determine the therapeutic levels of metoprolol enantiomers in both human plasma and saliva samples successfully, which can aid in therapeutic drug monitoring in clinical laboratories. Copyright © 2016 John Wiley & Sons, Ltd., (Copyright © 2016 John Wiley & Sons, Ltd.)
- Published
- 2016
- Full Text
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9. Ex vivo recapitulation of trauma-induced coagulopathy and preliminary assessment of trauma patient platelet function under flow using microfluidic technology.
- Author
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Li R, Elmongy H, Sims C, and Diamond SL
- Subjects
- Adult, Blood Coagulation Disorders blood, Blood Coagulation Disorders diagnosis, Female, Humans, Male, Platelet Function Tests, Wounds and Injuries blood, Blood Coagulation physiology, Blood Coagulation Disorders etiology, Blood Platelets physiology, Flow Cytometry methods, Wounds and Injuries complications
- Abstract
Background: Relevant to trauma-induced coagulopathy diagnostics, microfluidic assays allow controlled hemodynamics for testing of platelet and coagulation function using whole blood., Methods: Hemodilution or hyperfibrinolysis was studied under flow with modified healthy whole blood. Furthermore, platelet function was also measured using whole blood from trauma patients admitted to a Level I trauma center. Platelet deposition was measured with PPACK-inhibited blood perfused over collagen surfaces at a wall shear rate of 200 s, whereas platelet/fibrin deposition was measured with corn trypsin inhibitor-treated blood perfused over tissue factor (TF)/collagen., Results: In hemodilution studies, PPACK-treated blood displayed almost no platelet deposition when diluted to 10% hematocrit with saline, platelet-poor plasma, or platelet-rich plasma. Using similar dilutions, platelet/fibrin deposition was essentially absent for corn trypsin inhibitor-treated blood perfused over TF/collagen. To mimic hyperfibrinolysis during trauma, exogenous tissue plasminogen activator (50 nM) was added to blood before perfusion over TF/collagen. At both venous and arterial flows, the generation and subsequent lysis of fibrin were detectable within 6 minutes, with lysis blocked by addition of the plasmin inhibitor, ε-aminocaproic acid. Microfluidic assay of PPACK-inhibited whole blood from trauma patients revealed striking defects in collagen response and secondary platelet aggregation in 14 of 21 patients, whereas platelet hyperfunction was detected in three of 20 patients., Conclusion: Rapid microfluidic detection of (1) hemodilution-dependent impairment of clotting, (2) clot instability because of lysis, (3) blockade of fibrinolysis, or (4) platelet dysfunction during trauma may provide novel diagnostic opportunities to predict trauma-induced coagulopathy risk.
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- 2016
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10. Online post-column solvent assisted and direct solvent-assisted electrospray ionization for chiral analysis of propranolol enantiomers in plasma samples.
- Author
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Elmongy H, Ahmed H, Wahbi AA, Koyi H, and Abdel-Rehim M
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- Adrenergic beta-Antagonists chemistry, Chromatography, Liquid methods, Humans, Propranolol chemistry, Solvents, Spectrometry, Mass, Electrospray Ionization methods, Stereoisomerism, Tandem Mass Spectrometry methods, Adrenergic beta-Antagonists blood, Propranolol blood
- Abstract
An Online post-column solvent-assisted ionization (OPSAI) method was developed for enhancing the ionization of the beta-blocker propranolol utilizing normal phase LC-MS/MS. Solvent-assisted electrospray ionization (SAESI) was studied by the introduction of the assistant solvents A: 0.5% Formic acid in Isopropanolol, B: 0.5% Formic acid in Isopropanolol-Water (1:1), and C: 0.5% Formic acid in water into the electrospray ionization chamber using a spray needle. Analyte molecules can be directly ionized by the aid of the assistant solvent spray. Both methods were applied to the chiral separation of propranolol enantiomers using normal phase analysis on cellulose-based chiral column. Interestingly, both methods are easy to handle and offer a wide range of assistant solvents that can be used in order to gain the optimum ionization of the analyte molecules. The both methods considerably improved the analyte signal and the peak area greatly increased. The propranolol average signal-to-noise (S/N) ratio was enhanced from 26±1 and 42±1 to 2341±61 and 1725±29 for R-propranolol and S-propranolol, respectively, when the post-column solvent method (OPSAI) was used with isopropanol-assistant solvent (A). While in case of solvent-assisted electrospray ionization method (SAESI) signal was enhanced from 26±1 and 42±1 to 2223±72 and 2155±58 for R-propranolol and S-propranolol, respectively, with water as an assistant solvent. The limit of detection was 10ng/mL and the method was linear in the range 50-2000ng/mL. The NPLC-MS method was applied for the determination of propranolol enantiomers in human plasma after microextraction by packed C18 sorbent., (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Published
- 2015
- Full Text
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