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Nanoenabled strategies have recently attracted attention as a sustainable platform for agricultural applications. Here, we present a mechanistic understanding of nanobiointeraction through an orthogonal investigation. Pristine (nS) and stearic acid surface-modified (cS) sulfur nanoparticles (NPs) as a multifunctional nanofertilizer were applied to tomato ( Solanum lycopersicum L.) through soil. Both nS and cS increased root mass by 73% and 81% and increased shoot weight by 35% and 50%, respectively, compared to the untreated controls. Bulk sulfur (bS) and ionic sulfate (iS) had no such stimulatory effect. Notably, surface modification of S NPs had a positive impact, as cS yielded 38% and 51% greater shoot weight compared to nS at 100 and 200 mg/L, respectively. Moreover, nS and cS significantly improved leaf photosynthesis by promoting the linear electron flow, quantum yield of photosystem II, and relative chlorophyll content. The time-dependent gene expression related to two S bioassimilation and signaling pathways showed a specific role of NP surface physicochemical properties. Additionally, a time-dependent Global Test and machine learning strategy applied to understand the NP surface modification domain metabolomic profiling showed that cS increased the contents of IA, tryptophan, tomatidine, and scopoletin in plant leaves compared to the other treatments. These findings provide critical mechanistic insights into the use of nanoscale sulfur as a multifunctional soil amendment to enhance plant performance as part of nanoenabled agriculture.

Limited data exist on how surface charge and morphology impact the effectiveness of nanoscale copper oxide (CuO) as an agricultural amendment under field conditions. This study investigated the impact of these factors on tomatoes and watermelons following foliar treatment with CuO nanosheets (NS-) or nanospikes (NP+ and NP-) exhibiting positive or negative surface charge. Results showed plant species-dependent benefits. Notably, tomatoes infected with Fusarium oxysporum had significantly reduced disease progression when treated with NS-. Watermelons benefited similarly from NP+. Although disease suppression was significant and trends indicated increased yield, the yield effects weren't statistically significant. However, several nanoscale treatments significantly enhanced the fruit's nutritional value, and this nano-enabled biofortification was a function of particle charge and morphology. Negatively charged nanospikes significantly increased the Fe content of healthy watermelon and tomato (20-28 %) and Ca in healthy tomato (66 %), compared to their positively charged counterpart. Negatively charged nanospikes also outperformed negatively charged nanosheets, leading to significant increases in the content of S and Mg in infected watermelon (37-38 %), Fe in healthy watermelon (58 %), and Ca (42 %) in healthy tomato. These findings highlight the potential of tuning nanoscale CuO chemistry for disease suppression and enhanced food quality under field conditions., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

In the current study, foliar spray with lanthanum (La) based nanomaterials (La 10 Si 6 O 27 nanorods, La 10 Si 6 O 27 nanoparticle, La(OH) 3 nanorods, and La 2 O 3 nanoparticle) suppressed the occurrence of sheath blight ( Rhizoctonia solani ) in rice. The beneficial effects were morphology-, composition-, and concentration-dependent. Foliar application of La 10 Si 6 O 27 nanorods (100 mg/L) yielded the greatest disease suppression, significantly decreasing the disease severity by 62.4% compared with infected controls; this level of control was 2.7-fold greater than the commercially available pesticide (Thifluzamide). The order of efficacy was as follows: La 10 Si 6 O 27 nanorods > La 10 Si 6 O 27 nanoparticle > La(OH) 3 nanorods > La 2 O 3 nanoparticle. Mechanistically, (1) La 10 Si 6 O 27 nanorods had greater bioavailability, slower dissolution, and simultaneous Si nutrient benefits; (2) transcriptomic and metabolomic analyses revealed that La 10 Si 6 O 27 nanorods simultaneously strengthened rice systemic acquired resistance, physical barrier formation, and antioxidative systems. Additionally, La 10 Si 6 O 27 nanorods improved rice yield by 35.4% and promoted the nutritional quality of the seeds as compared with the Thifluzamide treatment. A two-year La 10 Si 6 O 27 nanorod exposure had no effect on soil health based on the evaluated chemical, physical, and biological soil properties. These findings demonstrate that La based nanomaterials can serve as an effective and sustainable strategy to safeguard crops and highlight the importance of nanomaterial composition and morphology in terms of optimizing benefit.

In this study, zinc and copper oxide nanoparticles (NPs) were synthesized using hemp (Cannabis sativa L.) leaves (ZnONP-HL and CuONP-HL), and their antifungal potential was assessed against Fusarium virguliforme in soybean (Glycine max L.). Hemp was selected because it is known to contain large quantities of secondary metabolites that can potentially enhance the reactivity of NPs through surface property modification. Synthesizing NPs with biologically derived materials allows to avoid the use of harsh and expensive synthetic reducing and capping agents. The ZnONP-HL and CuONP-HL showed average grain/crystallite size of 13.51 nm and 7.36 nm, respectively. The biologically synthesized NPs compared well with their chemically synthesized counterparts (ZnONP chem, and CuONP chem; 18.75 nm and 10.05 nm, respectively), confirming the stabilizing role of hemp-derived biomolecules. Analysis of the hemp leaf extract and functional groups that were associated with ZnONP-HL and CuONP-HL confirmed the presence of terpenes, flavonoids, and phenolic compounds. Biosynthesized NPs were applied on soybeans as bio-nano-fungicides against F. virguliforme via foliar treatments. ZnONP-HL and CuONP-HL at 200 μg/mL significantly (p < 0.05) increased (∼ 50%) soybean growth, compared to diseased controls. The NPs improved the nutrient (e.g., K, Ca, P) content and enhanced photosynthetic indicators of the plants by 100-200%. A 300% increase in the expression of soybean pathogenesis related GmPR genes encoding antifungal and defense proteins confirmed that the biosynthesized NPs enhanced disease resistance against the fungal phytopathogen. The findings from this study provide novel evidence of systemic suppression of fungal disease by nanobiopesticides, via promoting plant defense mechanisms., Competing Interests: Declaration of Competing Interest None., (Copyright © 2023 Elsevier Inc. All rights reserved.)

The use of nanotechnology to suppress crop diseases has attracted significant attention in agriculture. The present study investigated the antifungal mechanism by which aloe vera extract gel-biosynthesized (AVGE) selenium nanoparticles (Se NPs) suppressed Fusarium -induced wilt disease in lettuce ( Lactuca sativa ). AVGE Se NPs were synthesized by utilizing sodium selenite as a Se source and AVGE as a biocompatible capping and reducing agent. Over 21 d, 2.75% of total AVGE Se NPs was dissolved into Se ions, which was more than 8-fold greater than that of bare Se NPs (0.34%). Upon exposure to soil applied AVGE Se NPs at 50 mg/kg, fresh shoot biomass was significantly increased by 61.6 and 27.8% over the infected control and bare Se NPs, respectively. As compared to the infected control, the shoot levels of citrate, isocitrate, succinate, malate, and 2-oxo-glutarate were significantly upregulated by 0.5-3-fold as affected by both Se NPs. In addition, AVGE Se NPs significantly increased the shoot level of khelmarin D, a type of coumarin, by 4.40- and 0.71-fold over infected controls and bare Se NPs, respectively. Additionally, AVGE Se NPs showed greater upregulation of jasmonic acid and downregulation of abscisic acid content relative to bare Se NPs in diseased shoots. Moreover, the diversity of bacterial endophytes was significantly increased by AVGE Se NPs, with the values of Shannon index 40.2 and 9.16% greater over the infected control and bare Se NPs. Collectively, these findings highlight the significant potential of AVGE Se NPs as an effective and biocompatible strategy for nanoenabled sustainable crop protection.

Crop disease represents a serious and increasing threat to global food security. Lanthanum oxide nanomaterials (La 2 O 3 NMs) with different sizes (10 and 20 nm) and surface modifications (citrate, polyvinylpyrrolidone [PVP], and poly(ethylene glycol)) were investigated for their control of the fungal pathogen Fusarium oxysporum (Schl.) f. sp cucumerinum Owen on six-week-old cucumber ( Cucumis sativus ) in soil. Seed treatment and foliar application of the La 2 O 3 NMs at 20-200 mg/kg (mg/L) significantly suppressed cucumber wilt (decreased by 12.50-52.11%), although the disease control efficacy was concentration-, size-, and surface modification-dependent. The best pathogen control was achieved by foliar application of 200 mg/L PVP-coated La 2 O 3 NMs (10 nm); disease severity was decreased by 67.6%, and fresh shoot biomass was increased by 49.9% as compared with pathogen-infected control. Importantly, disease control efficacy was 1.97- and 3.61-fold greater than that of La 2 O 3 bulk particles and a commercial fungicide (Hymexazol), respectively. Additionally, La 2 O 3 NMs application enhanced cucumber yield by 350-461%, increased fruit total amino acids by 295-344%, and improved fruit vitamin content by 65-169% as compared with infected controls. Transcriptomic and metabolomic analyses revealed that La 2 O 3 NMs: (1) interacted with calmodulin, subsequently activating salicylic acid-dependent systemic acquired resistance; (2) increased the activity and expression of antioxidant and related genes, thereby alleviating pathogen-induced oxidative stress; and (3) directly inhibited in vivo pathogen growth. The findings highlight the significant potential of La 2 O 3 NMs for suppressing plant disease in sustainable agriculture.

Little is known about the effect of nano sulfur (NS) under field conditions as a multifunctional agricultural amendment. Pristine and surface coated NS (CS) were amended in soil at 200 mg/kg that was planted with tomato ( Solanum lycopersicum ) and infested with Fusarium oxysporum f. sp. lycopersici . Foliar exposure of CS (200 μg/mL) was also included. In healthy plants, CS increased tomato marketable yield up to 3.3∼3.4-fold compared to controls. In infested treatments, CS significantly reduced disease severity compared to the other treatments. Foliar and soil treatment with CS increased yield by 107 and 192% over diseased controls, respectively, and significantly increased fruit Ca, Cu, Fe, and Mg contents. A $33/acre investment in CS led to an increase in marketable yield from 4920 to 11,980 kg/acre for healthy plants and from 1135 to 2180 kg/acre for infested plants, demonstrating the significant potential of this nanoenabled strategy to increase food production.

Nanoscale sulfur can be a multifunctional agricultural amendment to enhance crop nutrition and suppress disease. Pristine (nS) and stearic acid coated (cS) sulfur nanoparticles were added to soil planted with tomatoes ( Solanum lycopersicum ) at 200 mg/L soil and infested with Fusarium oxysporum . Bulk sulfur, ionic sulfate, and healthy controls were included. Orthogonal end points were measured in two greenhouse experiments, including agronomic and photosynthetic parameters, disease severity/suppression, mechanistic biochemical and molecular end points including the time-dependent expression of 13 genes related to two S bioassimilation and pathogenesis-response, and metabolomic profiles. Disease reduced the plant biomass by up to 87%, but nS and cS amendment significantly reduced disease as determined by area-under-the-disease-progress curve by 54 and 56%, respectively. An increase in planta S accumulation was evident, with size-specific translocation ratios suggesting different uptake mechanisms. In vivo two-photon microscopy and time-dependent gene expression revealed a nanoscale-specific elemental S bioassimilation pathway within the plant that is separate from traditional sulfate accumulation. These findings correlate well with time-dependent metabolomic profiling, which exhibited increased disease resistance and plant immunity related metabolites only with nanoscale treatment. The linked gene expression and metabolomics data demonstrate a time-sensitive physiological window where nanoscale stimulation of plant immunity will be effective. These findings provide mechanistic understandings of nonmetal nanomaterial-based suppression of plant disease and significantly advance sustainable nanoenabled agricultural strategies to increase food production.

Seven Arabidopsis thaliana mutants with differences in cuticle thickness and stomatal density were foliar exposed to 50 mg L -1 Cu 3 (PO 4 ) 2 nanosheets (NS), CuO NS, CuO nanoparticles, and CuSO 4 . Three separate fractions of Cu (surface-attached, cuticle, interior leaf) were isolated from the leaf at 0.25, 2, 4, and 8 h. Cu transfer from the surface through the cuticle and into the leaf varied with mutant and particle type. The Cu content on the surface decreased significantly over 8 h but increased in the cuticle. Cu derived from the ionic form had the greatest cuticle concentration, suggesting greater difficulty in moving across this barrier and into the leaf. Leaf Cu in the increased-stomatal mutants was 8.5-44.9% greater than the decreased stomatal mutants, and abscisic acid to close the stomata decreased Cu in the leaf. This demonstrates the importance of nanomaterial entry through the stomata and enables the optimization of materials for nanoenabled agriculture.

In this study, we investigated the effects of citric acid (CA) coated copper oxide nanoparticles (CuO NPs) and their application method (foliar or soil exposure) on the growth and physiology of soybean (Glycine max). After nanomaterials exposure via foliar or soil application, Cu concentration was elevated in the roots, leaves, stem, pod, and seeds; distribution varied by plant organ and surface coating. Foliar application of CuO NPs at 300 mg/L and CuO-CA NPs at 75 mg/L increased soybean yield by 169.5% and 170.1%, respectively. In contrast, foliar and soil exposure to ionic Cu with all treatments (75 and 300 mg/L) had no impact on yield. Additionally, CuO-CA NPs at 300 mg/L significantly decreased Cu concentration in seeds by 46.7%, compared to control, and by 44.7%, compared to equivalent concentration of CuO NPs. Based on the total Cu concentration, CuO NPs appeared to be more accessible for plant uptake, compared to CuO-CA NPs, inducing a decrease in protein content by 56.3% and inhibiting plant height by 27.9% at 300 mg/kg under soil exposure. The translocation of Cu from leaf to root and from the root to leaf through the xylem was imaged by two-photon microscopy. The findings indicate that citric acid coating reduced CuO NPs toxicity in soybean, demonstrating that surface modification may change the toxic properties of NPs. This research provides direct evidence for the positive effects of CuO-CA NPs on soybean, including accumulation and in planta transfer of the particles, and provides important information when assessing the risk and the benefits of NP use in food safety and security., (Copyright © 2022 Elsevier B.V. All rights reserved.)

After use, a large number of engineered materials (ENMs) are directly or indirectly released into the environment. This may threaten the agricultural ecosystem, especially with crops under high demand for irrigation water, such as rice (Oryza sativa L.), a crop that feeds nearly half of the world's population. However, consistent and detailed information on the effects of nanoparticles in rice is limited. This review is a systematic exploration of the effects of ENMs on rice, with a critical evaluation of the mechanisms reported in the literature by which different nanomaterials cause toxicity in rice. The physiological and biochemical effects engendered by the nanoparticles are highlighted, focusing on rice growth and development, ENMs uptake and translocation, gene expression changes, enzyme activity modifications, and secondary metabolite alterations., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Micronutrients applied as nanoparticles of metal oxides have shown efficacy in vegetable and other crops for improving yield and reducing Fusarium diseases, but their role in ornamental crop management has not been investigated. In 2017, 2018, and 2020, nanoparticles of CuO, Mn 2 O 3 , or ZnO were foliarly applied at 500 μg/mL (0.6 mg/plant) to chrysanthemum transplants and planted in potting soil noninfested or infested with Fusarium oxysporum f. sp. chrysanthemi . An untreated control and a commercial fungicide, Fludioxonil, was also included. Chrysanthemums treated with nanoscale CuO had a 55, 30, and 32% reduction in disease severity ratings compared to untreated plants in 2017, 2018, and 2020, respectively. Specifically, the average dry biomass for the three years was reduced 22% by disease, but treatment with nanoscale CuO led to a 23% increase when compared to controls. Similar trends with plant height were observed. Horticultural quality was improved 28% with nano CuO and was equal to the fungicide. Nanoscale Mn 2 O 3 and the fungicide did not consistently reduce disease ratings or increase dry biomass each year. Nanoscale ZnO was ineffective. Nanoscale CuO-treated plants had 24 to 48% more Cu/g tissue than controls ( P < 0.001). These findings agree with past reports on food crops where single applications of nanoscale CuO improved plant health, growth, and yield and could offer significant impacts for managing plant diseases on ornamentals.

Scientific communication is facilitated by a data-driven, scientifically sound taxonomy that considers the end-user's needs and established successful practice. In 2013, the Fusarium community voiced near unanimous support for a concept of Fusarium that represented a clade comprising all agriculturally and clinically important Fusarium species, including the F. solani species complex (FSSC). Subsequently, this concept was challenged in 2015 by one research group who proposed dividing the genus Fusarium into seven genera, including the FSSC described as members of the genus Neocosmospora , with subsequent justification in 2018 based on claims that the 2013 concept of Fusarium is polyphyletic. Here, we test this claim and provide a phylogeny based on exonic nucleotide sequences of 19 orthologous protein-coding genes that strongly support the monophyly of Fusarium including the FSSC. We reassert the practical and scientific argument in support of a genus Fusarium that includes the FSSC and several other basal lineages, consistent with the longstanding use of this name among plant pathologists, medical mycologists, quarantine officials, regulatory agencies, students, and researchers with a stake in its taxonomy. In recognition of this monophyly, 40 species described as genus Neocosmospora were recombined in genus Fusarium , and nine others were renamed Fusarium. Here the global Fusarium community voices strong support for the inclusion of the FSSC in Fusarium , as it remains the best scientific, nomenclatural, and practical taxonomic option available.

Verticillium wilt, caused by Verticillium dahliae , is one of the major diseases of eggplants. Nanoparticles (NPs) of CuO, Mn 2 O 3 , and ZnO were sprayed alone onto leaves of young eggplants and in different combinations and rates, and then seedlings were transplanted into soil infested with V. dahliae in the greenhouse and field between 2015 and 2018. All combinations of NPs were consistently less effective than CuO NPs applied alone at 500 µg/ml at increasing disease suppression, biomass, and fruit yield. CuO NPs were associated with an increase in fruit yield (17 and 33% increase) and disease suppression (28 and 22% reduction) in 2016 and 2017, respectively, when compared with untreated controls. However, this effect was negated in the greenhouse and field experiments when CuO NPs were combined with Mn 2 O 3 . Combining NPs of CuO with ZnO resulted in variable effects; amendments increased growth and suppressed disease in greenhouse experiments, but results were mixed in the field. Leaf tissue analyses from the greenhouse experiments showed that Cu concentration in leaves was reduced when CuO NPs were combined with other NPs, even when application rates were the same amount. A simple competition for entry sites may explain why combinations of CuO NPs and Mn 2 O 3 NPs reduced efficacy but does not explain the lack of inhibition between Cu and Zn. NPs of CuO performed better than their larger bulk equivalent, and studies on application rate found 500 µg/ml was optimal. No phytotoxicity, as determined, by leaf burning, necrotic spots, or dead apical buds was noted even at the highest combined rates of 1,500 µg/ml.

Drought (40% field moisture capacity), organic fertilizer (O-F; 10%), and nano vs. bulk-ZnO particles (1.7 vs. 3.5 mg Zn/kg) were assessed in soil to determine their interactive effects on wheat performance and nutrient acquisition. Drought significantly reduced (6%) chlorophyll levels, whereas nano and bulk-ZnO alleviated some stress, thereby increasing (14-16%) chlorophyll levels, compared to the control. O-F increased (29%) chlorophyll levels and counteracted Zn's effect. Drought delayed (3-days) panicle emergence; O-F, nano and bulk-ZnO each accelerated (5-days) panicle emergence under drought, relative to the control and absence of O-F. Drought reduced (51%) grain yield, while O-F increased (130%) yield under drought. Grain yield was unaffected by Zn treatment under drought but increased (88%) under non-drought condition with bulk-ZnO, relative to the control. Drought lowered (43%) shoot Zn uptake. Compared to the control, nano and bulk-ZnO increased (39 and 23%, respectively) shoot Zn in the absence of O-F, whereas O-F amendment enhanced (94%) shoot Zn. Drought increased (48%) grain Zn concentration; nano and bulk-ZnO increased (29 and 18%, respectively) grain Zn, relative to the control, and O-F increased (85%) grain Zn. Zn recovery efficiency was in the order O-F > nano-ZnO > bulk-ZnO, regardless of the water status. Grain Fe concentration was unaffected by drought, under which O-F significantly reduced grain Fe, and nano-ZnO significantly reduced grain Fe, in the absence of O-F. Nano and bulk-ZnO also significantly reduced grain Fe, with O-F amendment under drought. Drought can have dire consequences for food and nutrition security, with implications for human health. This study demonstrated that drought-induced effects in food crops can be partially or wholly alleviated by ZnO particles and Zn-rich O-F. Understanding the interactions of drought and potential mitigation strategies such as fertilization with Zn-rich organic manure and ZnO can increase options for sustaining food production and quality under adverse conditions., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2018 Elsevier B.V. All rights reserved.)

Zinc oxide nanoparticles (ZnO-NPs) hold promise as novel fertilizer nutrients for crops. However, their ultra-small size could hinder large-scale field application due to potential for drift, untimely dissolution or aggregation. In this study, urea was coated with ZnO-NPs (1%) or bulk ZnO (2%) and evaluated in wheat ( Triticum aestivum L.) in a greenhouse, under drought (40% field moisture capacity; FMC) and non-drought (80% FMC) conditions, in comparison with urea not coated with ZnO (control), and urea with separate ZnO-NP (1%) or bulk ZnO (2%) amendment. Plants were exposed to ≤ 2.17 mg/kg ZnO-NPs and ≤ 4.34 mg/kg bulk-ZnO, indicating exposure to a higher rate of Zn from the bulk ZnO. ZnO-NPs and bulk-ZnO showed similar urea coating efficiencies of 74-75%. Drought significantly (p ≤ 0.05) increased time to panicle initiation, reduced grain yield, and inhibited uptake of Zn, nitrogen (N), and phosphorus (P). Under drought, ZnO-NPs significantly reduced average time to panicle initiation by 5 days, irrespective of coating, and relative to the control. In contrast, bulk ZnO did not affect time to panicle initiation. Compared to the control, grain yield increased significantly, 51 or 39%, with ZnO-NP-coated or uncoated urea. Yield increases from bulk-ZnO-coated or uncoated urea were insignificant, compared to both the control and the ZnO-NP treatments. Plant uptake of Zn increased by 24 or 8% with coated or uncoated ZnO-NPs; and by 78 or 10% with coated or uncoated bulk-ZnO. Under non-drought conditions, Zn treatment did not significantly reduce panicle initiation time, except with uncoated bulk-ZnO. Relative to the control, ZnO-NPs (irrespective of coating) significantly increased grain yield; and coated ZnO-NPs enhanced Zn uptake significantly. Zn fertilization did not significantly affect N and P uptake, regardless of particle size or coating. Collectively, these findings demonstrate that coating urea with ZnO-NPs enhances plant performance and Zn accumulation, thus potentiating field-scale deployment of nano-scale micronutrients. Notably, lower Zn inputs from ZnO-NPs enhanced crop productivity, comparable to higher inputs from bulk-ZnO. This highlights a key benefit of nanofertilizers: a reduction of nutrient inputs into agriculture without yield penalities., (Copyright © 2020 Dimkpa, Andrews, Fugice, Singh, Bindraban, Elmer, Gardea-Torresdey and White.)

In this study, the impact of cerium oxide nanoparticles on the nutritional value of tomato ( Solanum lycopersicum ) fruit grown in soil infested with Fusarium oxysporum f. sp. lycopersici was investigated in a greenhouse pot study. Three-week old seedlings of Bonny Best tomato plants were exposed by foliar and soil routes to nanoparticle CeO 2 (NP CeO 2 ) and cerium acetate (CeAc) at 0, 50, and 250 mg/L and transplanted into pots containing a soil mixture infested with the Fusarium wilt pathogen. Fruit biomass, water content, diameter, and nutritional content (lycopene, reducing and total sugar) along with elemental composition, including Ce, were evaluated. Fruit Ce concentration was below the detection limit in all treatments. Foliar exposure to NP CeO 2 at 250 increased the fruit dry weight (67%) and lycopene content (9%) in infested plants, compared with the infested untreated control. Foliar exposure to CeAc at 50 mg/L reduced fruit fresh weight (46%) and water content (46%) and increased the fruit lycopene content by 11% via root exposure as compared with the untreated infested control. At 250 mg/L, CeAc increased fruit dry weight (94%), compared with the infested untreated control. Total sugar content decreased in fruits of infested plants exposed via roots to NP CeO 2 at 50 mg/kg (63%) and 250 mg/kg (54%), CeAc at 50 mg/kg (46%), and foliarly at 50 mg/L (50%) and 250 mg/L (50%), all compared with the infested untreated control. Plants grown in Fusarium -infested soil had decreased fruit dry weight (42%) and lycopene content (17%) and increased total sugar (60%) and Ca content (140%), when compared with the noninfested untreated control ( p ≤ 0.05). Overall, the data suggested minimal negative effects of NP CeO 2 on the nutritional value of tomato fruit while simultaneously suppressing Fusarium wilt disease.

Soybeans ( Glycine max ) (V3 stage) were sprayed once with nanoparticles (NPs) of AgO, B, CeO, CuO, MnO, MoO 3 , SiO, TiO, or ZnO and exposed to Fusarium virguliforme , the cause of sudden death syndrome. Up to 80% root rot was observed in greenhouse experiments. However, NP CuO, B, MoO 3 , or ZnO reduced the root rot severity by 17-25%. Infected roots and shoots had significant changes in B, Mg, P, S, Si, and Zn, but NP treatment restored levels to that of the healthy control. For example, the increased root Mg and Mn contents induced by disease were reversed by NP B and Mn amendments. In vitro assays found that the NPs did not inhibit the pathogen. This, along with the restoration of altered nutrient levels in the plant tissue, suggests that modulated plant nutrition increased disease defense. Treatment of seedlings with nanoscale micronutrients may be a new tool in promoting soybean health.

Drought is a major environmental event affecting crop productivity and nutritional quality, and potentially, human nutrition. This study evaluated drought effects on performance and nutrient acquisition and distribution in sorghum; and whether ZnO nanoparticles (ZnO-NPs) might alleviate such effects. Soil was amended with ZnO-NPs at 1, 3, and 5 mg Zn/kg, and drought was imposed 4 weeks after seed germination by maintaining the soil at 40% of field moisture capacity. Flag leaf and grain head emergence were delayed 6-17 days by drought, but the delays were reduced to 4-5 days by ZnO-NPs. Drought significantly (p < 0.05) reduced (76%) grain yield; however, ZnO-NP amendment under drought improved grain (22-183%) yield. Drought inhibited grain nitrogen (N) translocation (57%) and total (root, shoot and grain) N acquisition (22%). However, ZnO-NPs (5 mg/kg) improved (84%) grain N translocation relative to the drought control and restored total N levels to the non-drought condition. Shoot uptake of phosphorus (P) was promoted (39%) by drought, while grain P translocation was inhibited (63%); however, ZnO-NPs lowered total P acquisition under drought by 11-23%. Drought impeded shoot uptake (45%), grain translocation (71%) and total acquisition (41%) of potassium (K). ZnO-NP amendment (5 mg/kg) to drought-affected plants improved total K acquisition (16-30%) and grain K (123%), relative to the drought control. Drought lowered (32%) average grain Zn concentration; however, ZnO-NP amendments improved (94%) grain Zn under drought. This study represents the first evidence of mitigation of drought stress in full-term plants solely by exposure to ZnO-NPs in soil. The ability of ZnO-NPs to accelerate plant development, promote yield, fortify edible grains with critically essential nutrients such as Zn, and improve N acquisition under drought stress has strong implications for increasing cropping systems resilience, sustaining human/animal food/feed and nutrition security, and reducing nutrient losses and environmental pollution associated with N-fertilizers., (Copyright © 2019 Elsevier B.V. All rights reserved.)

Plant response to microelements exposure can be modulated based on particle size. However, studies are lacking on the roles of particle size and specific microelements in mixed exposure systems designed for plant nutrition, rather than toxicology. Here, an addition-omission strategy was used to address particle-size and element-specific effects in soybean exposed to a mixture of nano and bulk scale oxide particles of Zn (2 mg Zn/kg), Cu (1 mg Cu/kg) and B (1 mg B/kg) in soil. Compared to the control, mixtures of oxide particles of both sizes significantly (p < 0.05) promoted grain yield and overall (shoot and grain) Zn accumulation, but suppressed overall P accumulation. However, the mixed nano-oxides, but not the mixed bulk-oxides, specifically stimulated shoot growth (47%), flower formation (63%), shoot biomass (34%), and shoot N (53%) and K (42%) accumulation. Compared by particle size, omission of individual elements from the mixtures evoked significant responses that were nano or bulk-specific, including shoot growth promotion (29%) by bulk-B; inhibition (51%) of flower formation by nano-Cu; stimulation (57%) of flower formation by bulk-B; grain yield suppression (40%) by nano-Zn; B uptake enhancement (34%) by bulk-Cu; P uptake stimulation by nano-Zn (14%) or bulk-B (21%); residual soil N (80%) and Zn (42%) enhancement by nano-Cu; and residual soil Cu enhancement by nano-Zn (72%) and nano-B (62%). Zn was responsible for driving the agronomic (biomass and grain yield) responses in this soil, with concurrent ramifications for environmental management (N and P) and human health (Zn nutrition). Overall, compared to bulk microelements, nanoscale microelements played a greater role in evoking plant responses., (Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.)

This study evaluated weathered and fresh ZnO-nanoparticles and Zn-salt effects on nutrient acquisition and redistribution in wheat. Weathered and fresh ZnO-nanoparticles and Zn-salt significantly increased grain yield by 15% and 29%, respectively. Postharvest soil acidification indicated ZnO-nanoparticles dissolved during growth. Zn was significantly bioaccumulated from both Zn types, but with low root-to-shoot bioaccumulation efficiency: 24% and 20% for weathered nanoparticles and salt, and 48% and 30% for fresh nanoparticles and salt. Grain Zn content was increased 186% and 229% by weathered nanoparticles and salt, and 229% and 300% by fresh nanoparticles and salt. Shoot-to-grain translocation efficiency was high: 167% and 177% for weathered nanoparticles and salt, and 209% and 155% for fresh nanoparticles and salt. However, Zincon assay indicated grain Zn does not exist as ions. This study demonstrates that ZnO-nanoparticles and Zn-salt vary in their effects on nutrient acquisition in wheat, with relevance for biofortification of Zn for human nutrition.

The use of nanoparticles in plant protection may reduce pesticide usage and contamination and increase food security. In this study, three-week-old Solanum lycopersicum seedlings were exposed, by root or foliar pathways, to CeO 2 nanoparticles and cerium acetate at 50 and 250 mg/L prior to transplant into sterilized soil. One week later, the soil was inoculated with the fungal pathogen Fusarium oxysporum f. sp. lycopersici (1 g/kg), and the plants were cultivated to maturity in a greenhouse. Disease severity, biomass/yield, and biochemical and physiological parameters were analyzed in harvested plants. Disease severity was significantly reduced by 250 mg/L of nano-CeO 2 and CeAc applied to the soil (53% and 35%, respectively) or foliage (57% and 41%, respectively), compared with non-treated infested controls. Overall, the findings show that nano-CeO 2 has potential to suppress Fusarium wilt and improve the chlorophyll content in tomato plants.

This study evaluated the effects of ZnO nanoparticles (NP) or Zn salt amendment on sorghum yield, macronutrient use efficiency, and grain Zn-enrichment. Amendments were through soil and foliar pathways, under "low" and "high" levels of nitrogen, phosphorus, and potassium (NPK). In soil and foliar amendments, grain yield was significantly (p ≤ 0.05) increased by both Zn types, albeit insignificantly with soil-applied Zn at low NPK. Across NPK levels and Zn exposure pathways, both Zn types increased N and K accumulation relative to control plants. Compared to N and K, both Zn types had a mixed effect on P accumulation, depending on NPK level and Zn exposure pathway, and permitted greater soil P retention. Both Zn types significantly (p ≤ 0.05) increased grain Zn content, irrespective of exposure pathway. These findings suggest a nanoenabled strategy for enhancing crop productivity, grain nutritional quality, and N use efficiency based on Zn micronutrient amendments, with potential implications for improved human and environmental health.

Asparagus can suffer from a crown and root rot caused by Fusarium oxysporum f. sp. asparagi and F. proliferatum. The disease is exacerbated when allelopathic toxins from old, rotting asparagus crowns are present in the soil. To minimize the damage from the replant problem, three strategies were examined: (i) biochar, (ii) application of earthworms (Lumbricus terrestris), and (iii) leaf mold to serve as a compost mulch and food source for earthworms. In a greenhouse, asparagus transplants were grown in soil amended with pathogen-infested asparagus residues or in nonamended soil, then both types of soil were augmented with biochar, earthworms, the combination of biochar and earthworms, or no treatment. Biochar increased arbuscular mycorrhizae (AM) colonization by 170% and reduced the incidence of root lesions by 57%; however, plant weight was not affected by any of the soil treatments and there were no significant interactions among the main effects. In the absence of infested asparagus residues, biochar reduced plant growth by 32%. Field plots that had severe crown and root rot, along with two other fields that had never been planted to asparagus, were planted with asparagus crowns and treated with leaf mold mulch, earthworms plus leaf mold mulch, biochar, or biochar plus earthworms plus leaf mold mulch. Untreated plots served as the control treatment. One year later, asparagus roots sampled from plots in the two new fields had a threefold increase in AM colonization when treated with biochar compared with control plots. Biochar did not increase yield over the duration of the 2012 to 2014 harvests when compared with that of the control plots. No soil treatment affected root colonization by AM in the field where Fusarium crown and root rot was severe. Compared with the untreated control plots, the leaf mold mulch treatment applied alone increased the marketable yields in each year of harvest. Combining leaf mold with earthworms provided no added benefit. Soil amendment with leaf mulch alone may hold promise for improving asparagus production in newly planted asparagus fields.

Tripartite interactions are common and occur when one agent (an arthropod or pathogen) changes the host plant in a manner that alters the attack of the challenging agent. We examined herbivory from the purple marsh crab (Sesarma reticulatum) on Spartina alterniflora following exposure to drought or inoculation with Fusarium palustre in mecocosms in the greenhouse and in crab-infested creek banks along intertidal salt marshes. Initially, drought stress on S. alterniflora and disease from F. palustre were examined in the greenhouse. Then, a second challenger, the purple marsh crab, was introduced to determine how drought and disease from F. palustre affected the attraction and consumption of S. alterniflora. Plant height and shoot and root weights were reduced in plants subjected to severe drought treatment when compared with normally irrigated plants. When the drought treatment was combined with inoculation with F. palustre, plants were significantly more stunted and symptomatic, had less fresh weight, more diseased roots, and a greater number of Fusarium colonies growing from the roots (P < 0.001) than noninoculated plants. The effects were additive, and statistical interactions were not detected between drought and inoculation. Estimates of herbivory (number of grass blades cut or biomass consumption) by the purple marsh crab were significantly greater on drought-stressed, diseased plants than on healthy plants irrigated normally. Drought increased attraction to the purple marsh crab more than inoculation with F. palustre. However, when only mild drought conditions were imposed, plant consumption was greater on inoculated plants. Healthy, nonstressed transplants set into plots in crabinfested intertidal creek banks were grazed less each year than inoculated plants or plants that were exposed to drought. Several hypotheses relating to nutrition, chemotaxis, and visual attraction are presented to explain how stress from drought or disease might favor herbivory.

Salt marshes rank as the most productive ecosystems on the planet. Biomass production can be greater than 3 kg dry matter/m 2 /year, which is 40% more biomass than tropical rainforests produce. Salt marshes provide multiple benefits to mankind. For example, coastal communities receive protection from storm surges and wave erosion. Salt marshes absorb excess nitrogen and phosphorus from sewage and fertilizer run-off into rivers, which, in turn, prevents algal blooms and hypoxia in coastal waters. In addition, these unique ecosystems provide habitat and shelter for many hundreds of species of shellfish, finfish, migratory and sedentary birds, and other marine animals. Despite the richness in animal species, the intertidal marshes of the salt marsh ecosystem are dominated by only a few plant species. Of these, the most prevalent plant species in a marsh are the tall and short forms of smooth cordgrass (Spartina alterniflora). The first recorded account of a dieback in a U.S. salt marsh was in the early 1990s in the Florida panhandle where patches of Sp. alterniflora as large as 1 ha died. This article explores possible causes of Sudden Vegetation Dieback.

The epidemiology and strategies for management of Fusarium wilt of China aster (Callistephus chinensis) were studied in Connecticut and Florida, USA, by examining seed contamination, on-farm disease incidence, sanitation, host resistance, and various soil treatments. Five out of 25 commercial seed packages from three separate distribution companies assayed in Connecticut had seeds contaminated with the pathogen Fusarium oxysporum f. sp. callistephi. Farm surveys of two cut-flower farms in Connecticut had disease incidences of 32 and 58%, while in Florida, the incidence of the disease ranged from 0.002 to 71.2% in two cut-flower operations. All pathogenic isolates from seed and symptomatic plants in Connecticut were vegetatively compatible, suggesting a common origin. Pathogenic isolates from Florida and nonpathogenic isolates fell into different vegetative compatibility groups and may have had another origin. Sodium hypochlorite solutions (1%) eliminated the fungus from seeds and Styrofoam when applied as a soak or spray, respectively. Soil fumigation with methyl bromide + chloropicrin, 1,3-dichloropropene + chloropicrin, or metam sodium maintained Fusarium wilt at low levels at a Florida cut-flower production facility. Evaluations of disease resistance of 44 cultivars in the greenhouse identified eight cultivars with moderate resistance. Four cultivars were identified with moderate resistance in field trials and thus could serve as a source of resistant germplasm for future breeding programs. These findings should encourage growers to use sanitation protocols to prevent entry of the pathogen into their fields and to choose commercially available cultivars that have moderate resistance.

Bloodroot (Sanguinaria canadensis L [Papaveraceae]) is a native herbaceous perennial in eastern North America, found from Nova Scotia to Florida. Although it is a common wildflower, rhizomes of double-flowered forms are sold commercially. Rhizomes planted into a wooded area in Guilford, CT produced healthy stems and flowers for a few years and then began to collapse and die in 2008. The same symptoms were observed with a new planting in 2011. Initially, leaves were dull green and were more leathery than healthy leaves. Eventually, the leaves collapsed at the junction of the petioles and the rhizomes. Vascular discoloration, if present, was obscured by the red pigmentation in the rhizome. A Fusarium sp. sporulated on the discolored tissue at the junction between healthy and rotted tissue. Stem pieces were surface disinfested (0.53% NaClO for 1 min), rinsed, and placed on Peptone-PCNB agar (2) at room temperature for 5 days. Colonies originating from single spores were subcultured on carnation leaf agar (2) and identified as Fusarium oxysporum based on falcate, thin-walled, three-septate macroconida borne in monophialides on doliform conidiophores (2). Four rhizomes of double-flowered bloodroot were planted in potting mix in the greenhouse in October 2010; sprouts were observed in March 2011. Two plants were inoculated in March 2011 by drenching the soil with 100 ml of a conidial suspension (10 6 spores/ml) and two control plants were treated with deionized water. Two months later, the inoculated plants were smaller than the controls. The treated plants subsequently collapsed and F. oxysporum was reisolated. Control plants remained healthy and F. oxysporum was not isolated. DNA extracted from the F. oxysporum isolates was used to obtain partial sequences of the translational elongation factor 1-alpha (tef1) gene, which were then blasted against the GenBank database. We observed a 100% similarity with F. oxysporum f. sp. lycopersici. The bloodroot isolates were compared with a known F. oxysporum f. sp. lycopersici isolate for their ability to cause disease on 2-week-old tomato seedlings (cv. Brandywine), using pathogenicity tests as described above. The known F. oxysporum f. sp. lycopersici isolate caused severe wilt and stunting of the tomato seedlings, but the bloodroot isolate caused no symptoms in inoculated seedling compared with those not inoculated. These results suggest that there may be more hosts for isolates in the F. oxysporum f. sp. lycopersici species complex than previously thought (1). An isolate (O-2603) has been deposited at the Fusarium Research Laboratory at Pennsylvania State University, University Park. Since bloodroot is now being sold commercially as an ornamental, disease management strategies may be needed. To our knowledge, this is the first report of a Fusarium crown rot of bloodroot. References: (1).V. Edel-Hermann et al. Online publication. doi:10.1111/j.1365-3059.2011.02551.x. Plant Pathology, 2011. (2) J. Leslie and B. Summerell. The Fusarium Laboratory Manual. Blackwell Publishing, Ames, IA, 2006.

Pyrolyzed biomass waste, commonly called biochar, has attracted interest as a soil amendment. A commercial prototype biochar produced by fast pyrolysis of hardwood dust was examined in soils to determine if it could reduce the damaging effect of allelopathy on arbuscular mycorrhizal (AM) root colonization and on Fusarium crown and root rot of asparagus. In greenhouse studies, biochar added at 1.5 and 3.0% (wt/wt) to asparagus field soil caused proportional increases in root weights and linear reductions in the percentage of root lesions caused by Fusarium oxysporum f. sp. asparagi and F. proliferatum compared with a control. Concomitant with these effects was a 100% increase in root colonization by AM fungi at the 3.0% rate. Addition of aromatic acids (cinnamic, coumaric, and ferulic) that are known allelopathic agents affecting asparagus reduced AM colonization but the deleterious effects were not observed following the application of biochar at the higher rate. When dried, ground, asparagus root and crown tissues infested with Fusarium spp. were added to soilless potting mix at 0, 1, or 5 g/liter of potting mix and then planted with asparagus, there was a decrease in asparagus root weight and increase in disease at 1 g/liter of potting mix but results were inconsistent at the higher residue rate. However, when biochar was added at 35 g/liter of potting mix (roughly 10%, vol/vol), these adverse effects on root weight and disease were equal to the nontreated controls. A small demonstration was conducted in field microplots. Those plots amended with biochar (3.5% [wt/wt] soil) produced asparagus plants with more AM colonization in the first year of growth but, in the subsequent year, biochar-treated plants were reduced in size, possibly due to greater than average precipitation and the ability of biochar to retain moisture that, in turn, may have created conditions conducive to root rot. These studies provide evidence that biochar may be useful in overcoming the deleterious effects of allelopathic residues in replant soils on asparagus.

Sudden vegetation dieback (SVD) is the loss of smooth cordgrass (Spartina alterniflora) along intertidal creeks in salt marshes of the Atlantic and Gulf states. The underlying cause of SVD remains unclear, but earlier work suggested a contributing role for Fusarium spp. in Louisiana. This report investigated whether these or other Fusarium species were associated with S. alterniflora dieback in mid- to north-Atlantic states. Isolations from seven SVD sites yielded 192 isolates of Fusarium spp., with more than 75% isolated from aboveground tissue. Most isolates (88%) fell into two undescribed morphospecies (MS) distinguished from each other by macroconidial shape, phialide ontogeny and growth rates. Pathogenicity tests on wound-inoculated S. alterniflora stems and seedling roots revealed that isolates in MS1 were more virulent than those in MS2 but no single isolate caused plant mortality. No matches to known species of Fusarium were revealed by DNA sequence queries of translation elongation factor 1-α (tef1) sequences. A phylogenetic analysis of partial sequences of three genes, β-tubulin (β-tub), calmodulin (cal) and tef1, was conducted on representative isolates from MS1 (n = 20) and MS2 (n = 18); it provided strong evidence that the MS1 isolates form a clade that represents a heretofore undescribed species, which we designate Fusarium palustre sp. nov. Isolates from the more variable MS2 clustered with the F. incarnatum-equiseti species complex as F. cf. incarnatum. Although a strong association exists between both species and declining S. alterniflora in SVD sites, neither appears to play a primary causal role in SVD. However, our findings suggest that F. palustre might play an important secondary role in the ecological disruption of the salt marshes.

The role of earthworms in plant disease has received little attention. To address whether earthworms would affect the severity of Verticillium wilt of eggplant (Solanum melongena) in the field, we grew eggplants in experimental field plots that were naturally infested with Verticillium dahliae in 2005, 2006, and 2007. Three earthworm treatments were compared: (i) no treatment (untreated control), (ii) earthworm populations reduced via chemical eradicants (carbaryl or hot mustard) (reduced treatment), and (iii) earthworm populations increased by addition of adult Canadian nightcrawlers (Lumbricus terrestris, 11 earthworms per m 2 ) (augmented treatment). Compared to the untreated control, the estimates of the area under the disease progress curve (AUDPC) were reduced while estimates of the canopy growth curve (CGC) and the final plant weights were increased in plots augmented with earthworms in all 3 years. In 2 out of 3 years, eggplant yield (weight and number of fruit) was increased in plots augmented with earthworms. When a carbaryl drench was used to reduce earthworm numbers, the treatment resulted in plants with more disease than in the untreated controls in 2005. However, in 2005 and 2006, carbaryl-treated plants had larger CGC values and higher yield than in the untreated controls and were not significantly different from the augmented plots. When a hot mustard extraction procedure was used to reduce earthworm densities in 2007, plant growth, yield, and disease variables did not differ from the untreated control. Although the effects of reducing earthworms were variable and difficult to explain, our findings suggest that augmenting earthworm populations can suppress Verticillium wilt of eggplant, and strategies that increase earthworm numbers may contribute to disease suppression.

Fusarium avenaceum is a globally distributed fungus commonly isolated from soil and a wide range of plants. Severe outbreaks of crown and stem rot of the flowering ornamental, lisianthus (Eustoma grandiflorum), have been attributed to F. avenaceum. We sequenced portions of the translation elongation factor 1-alpha (tef) and beta-tubulin (benA) protein coding genes as well as partial intergenic spacer (IGS) regions of the nuclear ribosomal genes in 37 Fusarium isolates obtained from lisianthus and other host plants. Isolates that were previously identified morphologically as F. acuminatum were included as an outgroup. Phylogenetic analyses of tef, benA, and IGS sequences showed that F. avenaceum isolates were an exclusive group with strong bootstrap support and no significant incongruence among gene genealogies. Isolates from lisianthus were scattered within this clade and did not form distinct groups based on host species or locality. Pathogenicity tests of F. avenaceum isolates obtained from several other hosts showed an ability to cause disease on lisianthus, suggesting that F. avenaceum may be pathogenic on lisianthus regardless of its phylogenetic origin. These findings have management implications and suggest that any host that supports F. avenaceum may serve as a source of inoculum for lisianthus growers.

Earthworm densities have been regarded as reliable indicators of soil health, but their role in suppression of plant disease has not received much attention. Several greenhouse studies were done to determine if soils infested with soilborne pathogens and augmented with earthworms (Lumbricus terrestris) could reduce disease of susceptible cultivars of asparagus (Asparagus officinalis), eggplant (Solanum melongena), and tomato (Solanum lycopersicum). Soils planted with asparagus were infested with Fusarium oxysporum f. sp. asparagi and F. proliferatum, eggplant with Verticillium dahliae, and tomato with F. oxysporum f. sp. lycopersici Race 1. In each host-disease system, earthworm activity was associated with an increase in plant growth and a decrease in disease. In general, plant weights were increased 60 to 80% and estimates of disease (area under the disease progress curve, percent vascular discoloration, and percent root lesions) were reduced 50 to 70% when soils were augmented with earthworms. Soil dilutions on selective media revealed that densities of fluorescent pseudomonads and filamentous actinomycetes were consistently higher for rhizosphere soils augmented with earthworms. In the studies with Verticillium wilt of eggplant, compared to the controls, the densities of total bacteria and Mn-transforming microbes were reduced in the presence of earthworms while population densities of bacilli and Trichoderma spp. were not affected. Disease suppression may have been mediated through microbiological activity. These studies suggest that strategies to increase earthworm densities in soil should suppress soilborne diseases.

Healthy specimens of selected grasses were collected from salt marshes and grown in the greenhouse. Plants were inoculated with Meloidogyne spartinae to determine the host range of this nematode. After 12 weeks, Spartina alterniflora plants formed root galls in response to infection and increased M. spartinae populations. Spartina patens, Spartina cynosuroides, Juncus gerardii and Distichlis spicata were non-hosts. In order to determine the natural distribution of M. spartinae in dieback areas, S. alterniflora plants were sampled from transects adjacent to dieback areas in Madison, CT, at low tide. Plants were sampled at the top or the creek and at 1-m intervals to the lowest area of plant growth at the low tide water's edge. Five samples were taken over an elevation drop of 90 cm. Two transects were taken each day on 21 June and 5 July 2007, and one transect was taken on 31 October 2007. Meloidogyne spartinae galls per gram root were higher at the higher elevations. In late June and early July 2007, M. spartinae developed more quickly in the higher elevations, perhaps because peat and sediments were drier and warmer away from low tide water levels. The effects of M. spartinae on S. alterniflora and the role of the nematode in marsh decline and dieback in the northeast United States remain to be determined.

Disease symptoms were observed in a commercial nursery in the midwest on Coreopsis verticillata 'Moonbeam' during the summer of 2006. Plants in a roughly circular area in one field showed foliar necrosis, stem basal cankers, root rot, and eventually plant death. Vascular discoloration was noted in stems of affected plants. Sporulation typical of Fusarium oxysporum was observed on the surface of cankers. Five isolates of F. oxysporum (KR1, KR2, KR4, MDU, and MDL) were taxonomically identified from monosporic cultures obtained from surface-disinfested stems and roots. All five isolates were vegetatively compatible with each other. Two methods of inoculation were used. Method one (conidial drench) involved pouring 100 ml of conidial suspension (10 6 conidia per ml) into 10-cm pots containing one healthy 2-month-old division of the same cultivar that was obtained from a different nursery. Method two (millet infestation) involved mixing autoclaved millet seed that had been colonized by each isolate into potting mix (2.5 g/L of mix) prior to transplanting. Four plants were tested per isolate per method and controls received distilled water or autoclaved millet. After 3 months, only two isolates (KR1 and KR2) inoculated by conidial drench caused root rot, whereas all isolates inoculated by millet infestation caused wilt, root rot, and vascular discoloration, and all inoculated plants died after 3.5 months Controls remained healthy. The fungus was recovered and was vegetatively compatible with the original F. oxysporum isolates. The tef-α gene from two F. oxysporum isolates was sequenced, submitted to the Blast ID search at Pennsylvania State University (1), and found to belong to the F. oxysporum species complex. Two isolates (KR1 and KR 2) have been deposited at the Fusarium Research Center at Pennsylvania State University under deposition numbers O-2437 and O-2438. Because of the popularity of this coreopsis cultivar, this disease has the potential to cause significant economic loss in nurseries and landscape businesses. The affected nursery, however, has taken all precautions to avoid disseminating the pathogen. Reference: (1) D. M. Geiser et al. Eur. J. Plant Pathol. 110:473, 2004.

Isolates of two biologically and phylogenetically distinct species, referred to as Fusarium solani f. sp. cucurbitae race 1 (Fsc-1 = Nectria haematococca mating population I [MPI]) and F. solani f. sp. cucurbitae race 2 (Fsc-2 = N. haematococca mating population V [MPV]), were suspected of causing an outbreak of Fusarium foot and fruit rot of pumpkin during 2001 to 2003 in Connecticut, New York, Ohio, and Missouri. Both species affect the fruit, but Fsc-1 also affects the crown and causes a stem rot. In this study, 156 isolates from affected plants and from soil under diseased fruit that tentatively were identified morphologically as members of the F. solani species complex were assayed for pathogenicity on pumpkin seedlings and mature fruit. Results of the pathogenicity assay indicated that 81 of the isolates were Fsc-1. The remaining 74 isolates were either nonpathogenic or only weakly pathogenic on the fruit. A subset of 53 test isolates from soil and plants, plus reference isolates of Fsc-1 and Fsc-2 and an isolate from wheat reported to cause a seedling rot on cucurbits, were characterized phylogenetically by sequencing a portion of the translation elongation factor 1-α gene. A BLAST query of the FUSARIUM-ID database at Pennsylvania State University indicated that 42 of the 53 test isolates were Fsc-1, whereas none were typed as Fsc-2. A polymerase chain reaction assay for mating-type (MAT) idiomorph revealed that all of the Fsc-1 isolates were MAT-1-2, suggesting that the pathogen may be strictly clonal in the affected fields. These findings provide convincing evidence that the Fusarium foot and fruit rot outbreaks were incited by Fsc-1.

Meloidogyne spartinae (Rau & Fassuliotis, 1965) was described from roots of smooth cordgrass (Spartina alterniflora Loisel) in Florida, Georgia, North and South Carolina, New Jersey, and New York (1,2). Affected plants were sampled in declining saltwater marshes at the Cape Cod National Seashore in Wellfleet, MA in May 2006 and Hammonassett State Park in Madison, CT in August 2006. Plants in adjacent, healthy stands were also sampled. Females, males, juveniles, and eggs of nematodes identified as M. spartinae were visible in roots stained with acid fuschin or were dissected from terminal galls at the root apex and from pockets in the root cortex where no galling was evident. The circular to ovoid terminal galls typically stopped root elongation. Morphological characteristics were used to identify this nematode as M. spartinae. Mature females in the root cortex were visible under a discolored lesion that appeared to result from a split in the cortex, probably from female expansion during development. Females were oval to lemon shaped with the neck protruding markedly to one side. Females also exhibited protruding perineal regions. In terminal galls, females were oriented toward the root tip; however, in the root cortex they were oriented either toward the root tip or toward the crown, with no obvious pattern. Egg masses were not observed and the eggs were deposited freely inside the gall or root cortex. Second-stage juveniles were long (730.3 μm, n = 60) with an elongate tail terminus. Males (2,203 μm, n = 40) were present in galls containing females. No morphological differences were observed between nematodes from the terminal galls or root cortex. M. spartinae was widespread in declining and adjacent healthy S. alterniflora. To our knowledge, this is the first report of M. spartinae from Connecticut and Massachusetts and the first report of M. spartinae development within root cortical tissues without gall formation. The role of this nematode in the sudden wetland dieback phenomenon (3) is being investigated. References: (1) J. D. Eisenback and H. Hirschmann. Nematology 3:303, 2001. (2) G. J. Rau and G. Fassuliotis. Proc. Helminthol. Soc. Wash. 32:159, 1965. (3) E. C. Webb and I. A Mendelssohn. Am. J. Bot. 83:1429, 1996.

During 2003, 10% of the Hiemalis begonias (Begonia × hiemalis Fotsch) developed wilt symptoms in a commercial greenhouse in Connecticut. Foliage turned a dull green, and stems developed a dark watersoaked discoloration near the soil line and had vascular discoloration. Stems, petioles, and leaves collapsed and became covered with sporodochia of a Fusarium spp. Single conidia were isolated from sporodochia and cultured on carnation leaf agar (CLA) and potato dextrose agar for 10 days. Isolates resembled Fusarium oxysporum, but the profuse sporulation with minimal aerial mycelium and the rare occurrence of polyphialides was consistent with the description of F. foetens (2). A comparison of a partial sequence of the 1-α elongation factor gene showed a 100% match with F. foetens. Inocula from five isolates were grown on CLA, washed from the plate, and adjusted to 10 6 conidia per ml. Suspension (50 μl) was injected into stems of healthy 6-week-old Hiemalis begonias cv. Barkos (one plant per isolate). Controls received distilled water. After 4 weeks, all inoculated plants turned dark and collapsed, and the same fungus was reisolated from these plants. Control stems remained healthy. An isolate (O-2348) has been deposited at the Fusarium Research Center at Pennsylvania State University, University Park. F. foetens has recently been described in association with a new disease of Hiemalis begonias in Europe (1). References: (1) R. Schrage, Phytomedizinischen Gesellschaft 33:68, 2003. (2) H.-J. Schroers et al. Mycologia 96:393, 2004.

ABSTRACT The role of NaCl in suppression of Fusarium crown and root rot of asparagus was investigated in split root culture so the direct effects of NaCl on the root and rhizosphere could be separated from effects that were translocated to the nontreated root side. One side of the root system was exposed to 100 ml of 0, 0.5, or 1.0% NaCl, while the other side received deionized water. Both sides of the root system were inoculated with conidial suspensions of the pathogens Fusarium oxysporum and F. proliferatum. When plants were harvested and assayed, root lesions and CFUs of F. oxysporum or F. proliferatum per centimeter of root from both exposed and nonexposed roots decreased as the NaCl rate increased to 1.0%, but the reduction relative to the control was significantly greater on roots that were directly exposed to NaCl (51% reduction in root lesions) than on adjacent nonexposed roots (31% reduction in root lesions). On both sides of the root systems, disease suppression with NaCl was associated with increases in the rhizosphere densities of fluorescent pseudomonads and Mn-reducing bacteria in the rhizosphere soil. In addition, as the NaCl rate increased, root tissues had marked reductions in malic acid and amino acids while concentrations of Cl and Mn increased in equal proportions on both sides of the root system. Chloride ions were absorbed in greater amounts than Na ions, and were more mobile in the plant than Na. Plants treated with 1% NaCl (171 meq of Cl(-) per liter) had soil leachates 1 week later of 47 meq of Cl(-) per liter from pots exposed to NaCl, but in the adjacent nonexposed pots, the amount of Cl in the leachates slowly increased over the course of the study to 20 meq/liter, presumably through the root exudation. These findings suggest that suppression of Fusarium crown and root rot of asparagus with NaCl may be due to multiple mechanisms. Maximum suppression occurs when NaCl is directly applied to roots, but suppression still occurs on distal non-treated roots resulting from systemic mechanisms. The latter mechanism may be associated with a root-mediated alteration in the rhizobacteria.

Replanted asparagus fields commonly fail to produce a profitable stand due to alleopathic residues left behind from the previous asparagus crop, elevated densities of pathogenic Fusarium spp., and low densities of vesicular arbuscular mycorrhizae (VAM). Formononetin, a plant isoflavone that stimulates VAM spores to germinate, and sodium chloride (NaCl), a disease-suppressing amendment, were evaluated alone and in combination for their effect on reestablishing asparagus at two locations in abandoned asparagus fields. Greenhouse studies also were conducted with naturally and artificially infested soils. Formononetin was applied as a crown soak or soil drench, and NaCl was applied as a granular treatment. Feeder roots from soil cores sampled from field plots and from greenhouse transplants were assayed for colonization by VAM and for lesions caused by Fusarium oxysporum and F. proliferatum. Formononetin increased the number of VAM vesicles in roots from the field and greenhouse studies and reduced the percent root lesions caused by Fusarium spp. when compared with the nontreated controls. NaCl was more effective than formononetin in reducing the percentage of root lesions in both field and greenhouse experiment when compared with untreated plants but had no effect on VAM colonization. However, there was evidence that NaCl negated the effect of formononentin on VAM colonization. The NaCl treatment increased the May 2001 spear number by 15% and marketable spear weight by 23%. At one site, treatment with formononetin increased mean number of stalks per plant by 29% in 2000 and 14% in 2001. Both formononetin and NaCl improve growth and reduce disease of asparagus in replanted asparagus and may be useful in reestablishing asparagus in abandoned asparagus field.

The influence of soil densities of Fusarium oxysporum f. sp. cyclaminis on development of Fusarium wilt of cyclamen (Cyclamen persicum) was evaluated by adding increasing amounts of F. oxysporum f. sp. cyclaminis-colonized millet inoculum to potting mix planted to cyclamen. Additions of inoculum resulted in an increase in the CFU of F. oxysporum f. sp. cyclaminis per ml of potting mix and an increase in vascular discoloration of the corm of cyclamens after 10 weeks. A threshold of 5 × 10 4 CFU/ml of potting mix was needed to consistently cause vascular discoloration in the corms. Final fresh leaf weights declined to 30% of the leaf weights of the control when inoculum was added at 10 5 CFU/ml of potting mix. The effect of NaCl on Fusarium wilt was also examined, as anecdotal reports from cyclamen producers suggest that NaCl applications may improve plant growth. When NaCl was applied to potting mix at rates of 0.25 and 0.50 g/liter of potting mix, final fresh weights were greater and the area under the disease progress curve values were less than those of control plants. However, final disease severity was not affected. In the absence of the pathogen, leaf weights were greater when NaCl was added at 0.25 g/liter of potting mix. Disease development and the effectiveness of NaCl on disease were not affected by potting mix pH 5.1 to 7.2. Cyclamens grown in potting mix with a pH of 7.2 had chlorotic leaves, but with the addition of NaCl, the chlorosis was not observed. Leaf analyses showed that the addition of NaCl increased foliar levels of Na, Cl, and Mn, but decreased foliar levels of P, Ca, Mg, S, and B. Sodium chloride applied at 0.25 to 0.50 g/liter of potting mix had growth benefits, but disease suppression was marginal.

Twenty-six isolates of Colletotrichum gloeosporioides were isolated from diseased ornamental lupines (Lupinus spp. 'Russell Hybrids') in seven different nurseries in Connecticut from 1996 to 1998. Three isolates from New Hampshire, New York, and Utah were also included. All isolates identified were pathogenic on lupine and vegetatively compatible with each other. Representative isolates were compared to lupine isolates from Quebec, Canada and France (COL-1 group), and from Australia and France (COL-2 group). Both groups are responsible for causing anthracnose of ornamental and forage Lupinus spp. in these countries. The Connecticut isolates were vegetatively compatible with the isolates in the COL-2 group and had random amplified polymorphic DNA profiles consistent with isolates in the COL-2 group. Isolates in the COL-1 group were vegetatively compatible only with each other and had random amplified polymorphic DNA profiles that differed from the COL-2 group. Isolates in both COL-1 and COL-2 were sensitive to both benomyl and thiobendazole, but the COL-1 group could be distinguished as slightly more tolerant than the COL-2 group and the Connecticut isolates. These assays provided persuasive evidence that the isolates from Connecticut belong to COL-2 group. The introduction of this homogenous pathogen population in Connecticut is likely due to the importation of infested seeds.

Eggplants (cv. Midnite) were grown during 1996 to 1998 in soils naturally infested with Verti-cillium dahliae. Experimental plots were either mulched with black plastic or grown on bare ground and fertilized with either (NH 4 ) 2 SO 4 or Ca(NO 3 ) 2 at 224 kg of N per hectare each season. Compared to bare ground, mulch resulted in a 42 and 68% increase in 1996 and 1997, respectively. Compared to Ca(NO 3 ) 2 , (NH 4 ) 2 SO 4 fertilization increased yield by 22 and 18% in 1996 and 1997, respectively. In both years, there were no significant interactions between the mulch and fertilizer treatments on yield or on integrated estimates of the plant canopy growth curve or disease severity. However, the treatment effects were additive. When compared to bare ground, mulching increased the plant canopy 3 weeks after planting and reduced the percentage of symptomatic foliage 8 weeks after planting. Compared to Ca(NO 3 ) 2 , fertilization with (NH 4 ) 2 SO 4 increased the plant canopy after 6 weeks, but did not affect the percentage of symptomatic foliage. Mulching or (NH 4 ) 2 SO 4 fertilization increased the number of flowers compared to no mulching or Ca(NO 3 ) 2 fertilization, respectively. The rate of nitrogen fertilizer at planting or as a side-dress application did not affect growth or disease, but plants treated with (NH 4 ) 2 SO 4 were larger and had less symptoms than Ca(NO 3 ) 2 -treated plants. The use of black plastic mulch with (NH 4 ) 2 SO 4 fertilization complements one another and may reduce damage from Verticillium wilt on eggplant.

Sixty-seven samples of asymptomatic asparagus spears (avg. 26.5 spears per sample) were obtained during 1995 and 1996 from 22 retail markets in New Haven County, CT, from three commercial growers in Connecticut, and from experimental field plots in Connecticut and North Carolina. Surface-disinfested basal and apical segments were placed on media selective for Fusarium spp. Eight species of Fusarium were identified among 418 isolates from 1,776 spears. Of these, 30 and 62% were F. oxysporum and F. proliferatum, respectively. The incidence of Fusarium colonization was greater from the basal segments (15.5%) than from apical segments (8.1%). Spears shipped from Mexico, Peru, and North Carolina had higher incidence of colonization by F. proliferatum than spears shipped from California, Washington, or the northeastern United States. In both years, spears purchased during June had the highest incidence of F. proliferatum compared with other months. Since F. oxysporum and F. proliferatum are pathogens and infect asparagus crowns and roots, it is probable that infection originated in the field. The less frequent species were found slightly more often on apical segments than on basal segments.

Single potato plants (Solanum tuberosum) cv. Superior were grown in field microplots to evaluate the effects of spent mushroom compost or straw mulch on early dying of potato caused by Verticillium dahliae and/or Pratylenchus penetrans. Prior to planting, soil was fumigated, placed in field microplots, and then infested with pathogens. Area under the senescence progress curve (AUSPC) was greater for plots infested with V. dahliae and/or P. penetrans than for noninfested plots. Plants grown in soil infested with both pathogens developed symptoms earlier than plants grown in soil infested with a single pathogen. Both V. dahliae and P. penetrans reduced yields; the combination of pathogens reduced marketable tuber yields by 22 to 44% (A- and B-sized tubers). Although the total number of tubers of all sizes was not affected by cultural or pathogen treatments, the addition of compost increased marketable tuber weight and decreased AUSPC. Soil amendment with spent mushroom compost may be a means of reducing the effects of potato early dying and increasing tuber yield when one or both pathogens are present.

Single potato plants (Solanum tuberosum cv. Superior) were grown in microplots in soil that was fumigated and then infested with Verticillium dahliae, Pratylenchus penetrans, or both to evaluate the effects of these pathogens and of cultural treatments with spent mushroom compost or straw mulch on gas exchange of potato leaves. Photosynthesis and transpiration of terminal leaflets of a cohort of similar-aged leaves were measured once a week from the time of expansion until they senesced. Over all measurements, gas exchange per unit leaf area was less for plants in microplots infested with V. dahliae or P. penetrans than for those in uninfested plots. For leaves that expanded in early June, gas exchange was similar immediately after leaf expansion but declined more quickly when microplots were infested with one or both pathogens compared to no infestation. Overall, leaf gas exchange was increased by compost amendment but not affected by straw mulch. Compost amendment prevented some of the decline in gas exchange due to infestation by one or both pathogens. For leaves that expanded in July, compost increased the gas exchange immediately after expansion in both infested and non-infested plots.

The combined effects of rotation crops and nitrogen fertilizers were examined on the strawberry black root rot disease complex. In July 1995, microplots were filled with soil that had a history of strawberry black root rot and seeded with two types of oats (Avena strigosa 'Saia oats' or A. sativa 'Garry oats') or with sorgho-sudangrass (Sorghum bicolor × S. sudanense 'Triple S'). Microplots planted with 1-year-old 'Honeoye' strawberry crowns served as the controls. In May 1996, the crops were chopped and incorporated into the soil. The soil was re-planted with 1-year-old strawberry 'Honeoye' crowns and then fertilized with (NH 4 ) 2 SO 4 or Ca(NO 3 ) 2 at equivalent rates of N. Two months later, (NH 4 ) 2 SO 4 -treated plants had 36% more leaf area and 41% more runners than strawberries treated with Ca(NO 3 ) 2 Strawberries that had been pre- cropped with 'Saia' oats had 135% more runners and 38% more early fruit yield than strawberries grown in control microplots. Total fruit yield was not affected by the treatments. Compared to Ca(NO 3 ) 2 , the (NH 4 ) 2 SO 4 treatment reduced the percentage of blackened roots. The influence of the cover crops on growth and disease was stronger with (NH 4 ) 2 SO 4 fertilization than with Ca(NO 3 ) 2 fertilization. Combining 'Saia' oats or sorgho-sudangrass rotation with (NH 4 ) 2 SO 4 fertilization reduced lesion nematode (Pratylenchus penetrans) numbers in subsequent strawberry roots when compared to controls. Also, the combination of 'Saia' or 'Garry' oats as a pre-crop with applications of (NH 4 ) 2 SO 4 reduced black root rot severity when compared to controls. Only the 'Garry' oat rotation reduced strawberry root colonization by Rhizoctonia fragariae when compared to controls. Other effects were associated with using (NH 4 ) 2 SO 4 . The (NH 4 ) 2 SO 4 treatment lowered the rhizosphere soil pH by 0.2 units, reduced the numbers of fluorescent pseudomonads in the rhizosphere by 10- to 15-fold, and produced leaves that had more N, K, S, Mn, and Zn content than plants treated with Ca(NO 3 ) 2 . Rotation with 'Saia' oats combined with (NH 4 ) 2 SO 4 fertilization may suppress strawberry black root rot and increase yields through multiple effects on the host, pathogens, and associated microflora.

Growth chamber evaluation of several cultivars of basil and related herbs examined in the United States revealed that identical cultivars from different sources did not differ in their reactions to artificial inoculation with Fusarium oxysporum f. sp. basilicum. Cultivars differed in susceptibility to the pathogen: "Spicy globe" miniature was the most susceptible, and lemon basil (Ocimum basilicum var. citriodorum), Origanum majorana, and Thymus vulgaris were rated as not susceptible. Twenty isolates of F. oxysporum, originating from stems of diseased basil plants in Israel, were pathogenic on basil in growth chamber and greenhouse tests. Under artificial inoculation, 2 isolates of F. oxysporum f. sp. basilicum from stems were pathogenic to basil but not to 9 species representing Lamiaceae, Cucurbitaceae, Solanaceae, and Compositae indicating the specificity of the pathogen to basil. These isolates were used for additional resistance tests. Ocimum basilicum var. purpurascens (Exotic) and var. citriodorum were rated as not susceptible to the pathogen under artificial inoculation. Resistant germ plasm was identified in several basil plants of a local variety originally introduced from the United States and reselected at Newe Ya'ar. Seeds were planted in the greenhouse in naturally highly infested soil. Symptomless plants that survived in naturally infested soil were the source for F1 seeds of resistant germ plasm, which was confirmed by artificial inoculations with both isolates of the pathogen. Further selection tests to improve resistance were conducted up to the F4 generation in infested soil in the greenhouse. All individuals of the present genetic line remained symptomless, while all individual plants of the original susceptible cultivar defoliated 3 weeks after planting into infested soil, suggesting that the resistance may be a single, dominant gene. The causal organism was reisolated only from the susceptible plants and not from the symptomless resistant plants through all the experiments.