For decades, efforts have been made to reduce one of the most central causes of organizational stress, namely workload, which describes the amount or difficulty of employees’ work (Bowling & Kirkendall, 2012). With the rapid development of technology, newer and newer technologies are being developed to reduce workload, and thus counteract stress. New technologies such as software agents are increasingly used to perform complex tasks (O’Neill et al., 2022), and are supposed to decrease workload of humans (Wright et al., 2016; Bradshaw, 1997). Those software agents differ in their level of autonomy (LOA), and, consequently, in the degree to which they assist with or takeover tasks. Besides workload, there are other dangerous overload conditions for employees that can lead to negative consequences such as stress, namely information overload (IO; Graf & Antoni, 2022), which describes a state of being overwhelmed by information, where one perceives that information demands exceed one’s information processing capacity (Antoni & Ellwart, 2017). Whether the use of agents with different LOAs and their different task support only influences the workload, or also the information processing and therefore IO, has not yet been clarified. The more autonomous and thus the more the software agent takes over work, the less operational work is done by humans, which should be reflected in lower workload (Prewett et al., 2020). Research relates this decrease in workload with higher autonomy of robots and systems, but also unclear findings are present (Selkowitz et al., 2017; Omnasch et al., 2014). Moreover, information processing could decrease if the agent acts autonomously, and its processes are not followed. However, monitoring of the agents’ actions might create additional information load, because high, and/ or divided attention and therefore information processing is still needed (Wickens & Carswell, 2021). If the software agent acts at high speed with high autonomy, IO might even increase due to the fast-paced monitoring task. Therefore, different LOA could affect IO differently, since different amounts and complexities of information need to be incorporated depending on agent behavior. Research suggested that software agents may reduce human IO (Berghel, 1997; Bradshaw, 1997, Maes, 1997) - however, if the use of different LOAs of software agents leads to less information to be processed and thus there is less risk to perceive IO has not been clarified to date in a randomized-controlled study. Therefore, this research transfers workload and IO to the context of software agents in human-autonomy contexts and observes the (possible) differential influence of LOA. Both high workload and information overload (IO) are two processes established in research that represent an imbalance between environmental requirements and internal capacities (Graf & Antoni, 2022; Meijman & Mulder, 2013, p. 5-6). Stress perceptions can result from such an imbalance between external demands and internal regulatory capabilities (Koolhaas et al, 2011), particularly if loss of control and negative consequences are perceived. In the literature, mixed findings are present that show that higher LOA might increase the perception of technology related stress and exhaustion (Ulfert et al, 2022), decrease it (Pollak et al., 2020), or do not influence it at all (Sauer et al., 2013; Szalma & Taylor, 2011), despite that software agents may reduce workload (Omnasch et al., 2014). These mixed findings leave open, whether there is a higher experience of stress when using different LOA software agents, and what reasons lie behind this. Therefore, this study focusses on two research questions, namely, 1) how information overload is influenced by different LOA, and if these effects are differential from effects on workload, and 2) how stress perceptions (i.e., stress, technostrain) are influenced by different LOA, and if those effects differ.