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Carnosine (β-alanyl-L-histidine) and its methylated analogues anserine and balenine are highly concentrated endogenous dipeptides in mammalian skeletal muscle that are implicated in exercise performance. Balenine has a much better bioavailability and stability in human circulation upon acute ingestion, compared to carnosine and anserine. Therefore, ergogenic effects observed with acute carnosine and anserine supplementation may be even more pronounced with balenine. This study investigated whether acute balenine supplementation improves physical performance in four maximal and submaximal exercise modalities. A total of 20 healthy, active volunteers (14 males; six females) performed cycling sprints, maximal isometric contractions, a 4-km TT and 20-km TT following either preexercise placebo or 10 mg/kg of balenine ingestion. Physical, as well as mental performance, along with acid–base balance and glucose concentration were assessed. Balenine was unable to augment peak power (p = .3553), peak torque (p = .3169), time to complete the 4 km (p = .8566), nor 20 km time trial (p = .2660). None of the performances were correlated with plasma balenine or CN1 enzyme activity. In addition, no effect on pH, bicarbonate, and lactate was observed. Also, the supplement did not affect mental performance. In contrast, glucose remained higher during and after the 20 km time trial following balenine ingestion. In conclusion, these results overall indicate that the functionality of balenine does not fully resemble that of carnosine and anserine, since it was unable to elicit performance improvements with similar and even higher plasma concentrations.

Purpose: To examine whether the muscle typology of elite and world-class swimmers could discriminate between their best distance event, swimming stroke style, or performance level. Methodology: The muscle carnosine content of 43 male (860 [76] FINA [Fédération Internationale de Natation] points) and 30 female (881 [63] FINA points) swimmers was measured in the soleus and gastrocnemius by proton magnetic resonance spectroscopy and expressed as a carnosine aggregate Z score (CAZ score) to estimate muscle typology. A higher CAZ score is associated with a higher estimated proportion of type II fibers. Swimmers were categorized by their best stroke, distance category (sprinters, 50–100 m; middle distance, 200–400 m; or long distance, 800 m–open water), and performance level (world-class, world top 10, or elite and world top 100 swimmers outside of the world top 10). Results: There was no significant difference in the CAZ score of sprint- (−0.08 [0.55]), middle- (−0.17 [0.70]), or long-distance swimmers (−0.30 [0.75], P = .693). World-class sprint swimmers (all strokes included) had a significantly higher CAZ score (0.37 [0.70]) when compared to elite sprint swimmers (−0.25 [0.61], P = .024, d = 0.94). Breaststroke swimmers (0.69 [0.73]) had a significantly higher CAZ score compared to freestyle (−0.24 [0.54], P d = 1.46), backstroke (−0.16 [0.47], P = .006, d = 1.42), and butterfly swimmers (−0.39 [0.53], P d = 1.70). Furthermore, within the cohort of breaststroke swimmers, there was a significant positive correlation between FINA points and CAZ score (r = .728, P = .011); however, this association was not evident in other strokes. Conclusion: While there was no clear association between muscle typology and event distance specialization, world-class sprint swimmers possess a greater estimated proportion of type II fibers compared to elite sprint swimmers, as well as breaststroke swimmers compared to freestyle, backstroke, and butterfly swimmers.

Noninvasive techniques to quantify metabolites in skeletal muscle provide unique insight into human physiology and enable the translation of research into practice. Proton magnetic resonance spectroscopy (1H-MRS) permits the assessment of several abundant muscle metabolites in vivo, including carnosine, a dipeptide composed of the amino acids histidine and beta-alanine. Muscle carnosine loading, accomplished by chronic oral beta-alanine supplementation, improves muscle function and exercise capacity and has pathophysiological relevance in multiple diseases. Moreover, the marked difference in carnosine content between fast-twitch and slow-twitch muscle fibers has rendered carnosine an attractive candidate to estimate human muscle fiber type composition. However, the quantification of carnosine with1H-MRS requires technical expertise to obtain accurate and reproducible data. In this review, we describe the technical and physiological factors that impact the detection, analysis, and quantification of carnosine in muscle with1H-MRS. We discuss potential sources of error during the acquisition and preprocessing of the1H-MRS spectra and present best practices to enable the accurate, reliable, and reproducible application of this technique.

Purpose: To determine the association between estimated muscle fiber typology and the start and turn phases of elite swimmers during competition. Methods: International and national competition racing performance was analyzed from 21 female (FINA points = 894 ± 39: 104.5 ± 1.8% world record ratio [WRR]) and 25 male (FINA points = 885 ± 54: 104.8 ± 2.1% WRR) elite swimmers. The start, turn, and turn out times were determined from each of the swimmers’ career best performance times (FINA points = 889 ± 48: 104.7 ± 2.0% WRR). Muscle carnosine concentration was quantified by proton magnetic resonance spectroscopy in the gastrocnemius and soleus and was expressed as a carnosine aggregate z score relative to an age- and gender-matched nonathlete control group to estimate muscle fiber typology. Linear mixed models were employed to determine the association between muscle fiber typology and the start and turn times. Results: While there was no significant influence of carnosine aggregate z score on the start and turn times when all strokes and distance events were entered into the model, the swimmers with a higher carnosine aggregate z score (ie, faster muscle typology) had a significantly faster start time in 100-m events compared with the swimmers with a lower carnosine aggregate z score (P = .02, F = 5.825). The start and turn times were significantly faster in the male compared with the female swimmers in the 100-m events compared with other distances, and between the 4 different swimming strokes (P Conclusion: This study suggests that start times in sprint events are partly determined (and limited) by muscle fiber typology, which is highly relevant when ∼12% of the overall performance time is determined from the start time.

The present study identified the physiological and performance characteristics that are deterministic during a maximal 1500-m time trial and in paced 1500-m time trials, with an all-out last lap. Thirty-two trained middle-distance runners (n = 21 male, VO2peak: 72.1 ± 3.2; n = 11, female, VO2peak: 61.2 ± 3.7 mL kg−1 min−1) completed a 1500-m time trial in the fastest time possible (1500FAST) as well as a 1500MOD and 1500SLOW trial whereby mean speed was reduced during the 0–1100 m by 5% and 10%, respectively. Anaerobic speed reserve (ASR), running economy (RE), the velocity corresponding with VO2peak (VVO2peak), maximal sprint speed (MSS) and maximal accumulated oxygen deficit (MAOD) were determined during additional testing. Carnosine content was quantified by proton magnetic resonance spectroscopy in the gastrocnemius and expressed as a Z-score to estimate muscle fibre typology. 1500FAST time was best explained by RE and VVO2peak in female runners (adjusted r2 = 0.80, P

Background : Hamstring strain injuries (HSI) are prevalent in team sports and occur frequently in the latter phase of matches. In the search for interindividual factors that determine muscle fatigue and possibly injury risk, muscle fibre typology is a likely candidate. It was the aim of the study to determine whether muscle fibre typology is a risk factor for HSI. Methods : A prospective cohort study was conducted over 3 seasons in professional football players competing in the Belgian Jupiler Pro League (n=118) and in the English Premier League (n=47), and a total of 27 hamstring strain injuries were sustained during this period. The muscle fibre typology was non-invasively estimated using proton magnetic resonance spectroscopy, and was characterized as a fast, slow, or intermediate typology based on the carnosine concentration in the soleus. A multivariate cox model was used to identify risk factors for HSI. Results : Football players exhibit a wide variety of muscle typologies (slow typology=44.9%; intermediate typology=39.8%, fast typology=15.3%). In the combined cohort, players with a fast typology displayed a 5.3-fold (95% CI 1.92-14.8, P=0.001) higher risk than slow typology players to sustain an index HSI. This was also independently observed in both leagues separately as respectively a 6.7-fold (95% CI 1.3-34.1; P=0.023) and a 5.1-fold (95% CI 1.2-20.4; P=0.023) higher chance was found in fast typology players than slow typology players of the Jupiler Pro League and the Premier League cohort. Conclusion : We discovered muscle fibre typology as a novel and potent risk factor for HSI in team sports.

Purpose: We aimed to identify the underpinning physiological and speed/mechanical determinants of different types of 800-m running time trials (i.e., with a positive or negative pacing strategy) and key components within each 800-m time trial (i.e., first and final 200-m). Methods: Twenty trained male 800-m runners (800-m personal best time (min:s): 1:55.10±0:04.44) completed a maximal 800-m time trial (800MAX) and one pacing trial, whereby runners were paced for the first lap and speed was reduced by 7.5% (800PACE) relative to 800MAX, while the last lap was completed in the fastest time possible. Anaerobic speed reserve, running economy, the velocity corresponding with VO2peak (VVO2peak), maximal sprint speed (MAXSS), maximal accumulated oxygen deficit and sprint force-velocity-power profiles were derived from laboratory and field testing. Carnosine content was quantified by proton magnetic resonance spectroscopy in the gastrocnemius and soleus and expressed as a carnosine aggregate Z-score (CAZ-score) to estimate muscle typology. Data were analysed using multiple stepwise regression analysis. Results: MAXSS and vVO2peak largely explained the variation in 800MAX time (r2 =0.570; P=0.020), while MAXSS was the best explanatory variable for the first 200-m time in 800MAX (adjusted r2 =0.661, P

Purpose: The aims of this study were 1) to model the temporal profile of W' recovery after exhaustion, 2) to estimate the contribution of changing (V) over dotO(2) kinetics to this recovery, and 3) to examine associations with aerobic fitness and muscle fiber type (MFT) distribution. Methods: Twenty-one men (age = 25 +/- 2 yr, (V) over dotO(2peak) = 54.4 +/- 5.3 mL.min(-1).kg(-1)) performed several constant load tests to determine critical power and W' followed by eight trials to quantify W' recovery. Each test consisted of two identical exhaustive work bouts (WB1 and WB2), separated by a variable recovery interval of 30, 60, 120, 180, 240, 300, 600, or 900 s. Gas exchange was measured and muscle biopsies were collected to determine MFT distribution. W' recovery was quantified as observed W' recovery (W'(OBS)), model-predicted W' recovery (W'(BAL)), and W' recovery corrected for changing (V) over dotO(2) kinetics (W'(ADJ)). W'(OBS) and W'(ADJ) were modeled using mono- and biexponential fitting. Root-mean-square error (RMSE) and Akaike information criterion (increment AIC(C)) were used to evaluate the models' accuracy. Results: The W'(BAL) model (tau = 524 +/- 41 s) was associated with an RMSE of 18.6% in fitting W'(OBS) and underestimated W' recovery for all durations below 5 min (P < 0.002). Monoexponential modeling of W'(OBS) resulted in tau = 104 s with RMSE = 6.4%. Biexponential modeling of W'(OBS) resulted in tau(1) = 11 s and tau(2) = 256 s with RMSE = 1.7%. W'(ADJ) was 11% +/- 1.5% lower than W'(OBS) (P < 0.001). increment AIC(C) scores favored the biexponential model for W'(OBS), but not for W'(ADJ). (V) over dotO(2peak) (P = 0.009) but not MFT distribution (P = 0.303) was associated with W'(OBS). Conclusion: We showed that W' recovery from exhaustion follows a two-phase exponential time course that is dependent on aerobic fitness. The appearance of a fast initial recovery phase was attributed to an enhanced aerobic energy provision resulting from changes in (V) over dotO(2) kinetics.

This study aimed to identify the relationships between lower limb muscle characteristics and mechanical variables derived from the vertical (jumping) and horizontal (sprinting) force-velocity-power (FVP) profiles.Nineteen subelite male rugby league players performed a series of squat jumps and linear 30-m sprints to derive the vertical and horizontal FVP profiles, respectively. The theoretical maximal force (F0), velocity (V0), and power (Pmax) were derived from both the vertical (i.e., vF0, vV0, and vPmax) and the horizontal (i.e., hF0, hV0, and hPmax) FVP profiles. Vastus lateralis (VL), biceps femoris long head, and gastrocnemius medialis (GM) and lateralis muscle fascicle length, pennation angle, and thickness were measured using B-mode ultrasonography. Magnetic resonance imaging was used to calculate volumes of major lower limb muscles, whereas proton magnetic resonance spectroscopy was used to quantify the carnosine content of the GM to estimate muscle fiber typology.Variation in vPmax was best explained by GM muscle fiber typology (i.e., greater estimated proportion of Type II fibers) and VL volume (adjusted r2 = 0.440, P = 0.006), whereas adductor and vastus medialis volume and GM muscle fiber typology explained the most variation in hPmax (adjusted r2 = 0.634, P = 0.032). Rectus femoris and VL volume explained variation in vF0 (r2 = 0.430, P = 0.008), whereas adductor and vastus medialis volume explained variation in hF0 (r2 = 0.432, P = 0.007). Variations in vV0 and hV0 were best explained by GM muscle fiber typology (adjusted r2 = 0.580, P0.001) and GM muscle fiber typology and biceps femoris short head volume (adjusted r2 = 0.590, P0.001), respectively.Muscle fiber typology and muscle volume are strong determinants of maximal muscle power in jumping and sprinting by influencing the velocity- and force-oriented mechanical variables.

PURPOSE Classic track-and-field studies demonstrated that elite endurance athletes exhibit a slow muscle typology, whereas elite sprint athletes have a predominant fast muscle typology. In elite cycling, conclusive data on muscle typology are scarce, which may be due to the invasive nature of muscle biopsies. The noninvasive estimation of muscle typology through the measurement of muscle carnosine enabled to explore the muscle typology of 80 world-class cyclists of different disciplines. METHODS The muscle carnosine content of 80 cyclists (4 bicycle motor cross racing [BMX], 33 track, 8 cyclo-cross, 24 road, and 11 mountain bike) was measured in the soleus and gastrocnemius by proton magnetic resonance spectroscopy and expressed as a z-score relative to a reference population. Track cyclists were divided into track sprint and endurance cyclists based on their Union Cycliste Internationale (UCI) ranking. Moreover, road cyclists were further characterized based on the percentage of UCI points earned during either single and multistage races. RESULTS BMX cyclists (carnosine aggregate z-score of 1.33) are characterized by a faster muscle typology than track, cyclo-cross, road, and mountain bike cyclists (carnosine aggregate z-score of -0.08, -0.76, -0.96, and -1.02, respectively; P < 0.05). Track cyclists also possess a faster muscle typology compared with mountain bikers (P = 0.033) and road cyclists (P = 0.005). Moreover, track sprinters show a significant faster muscle typology (carnosine aggregate z-score of 0.87) compared with track endurance cyclists (carnosine aggregate z-score of -0.44) (P < 0.001). In road cyclists, the higher the carnosine aggregate z-score, the higher the percentage of UCI points gained during single-stage races (r = 0.517, P = 0.010). CONCLUSIONS Prominent differences in the noninvasively determined muscle typology exist between elite cyclists of various disciplines, which opens opportunities for application in talent orientation and transfer.

Due to the invasiveness of a muscle biopsy, there is fragmentary information on the existence and possible origin of a sexual dimorphism in the skeletal muscle concentrations of the energy delivery-related metabolites carnosine, creatine, and carnitine. As these metabolites can be noninvasively monitored by proton magnetic resonance spectroscopy, this technique offers the possibility to investigate if sexual dimorphisms are present in an adult reference population and if these dimorphisms originated during puberty using a longitudinal design. Concentrations of carnosine, creatine, and carnitine were examined using proton magnetic resonance spectroscopy in the soleus and gastrocnemius muscles of an adult reference population of female (n = 50) and male adults (n = 50). For the longitudinal follow-up over puberty, 29 boys and 28 girls were scanned prepuberty. Six years later, 24 boys and 24 girls were rescanned postpuberty. A sexual dimorphism was present in carnosine and creatine, but not carnitine, in the adult reference population. Carnosine was 28.5% higher in the gastrocnemius (P < 0.001) and carnosine and creatine were respectively 19.9% (P < 0.001) and 18.2% (P < 0.001) higher in the soleus of male when compared with female adults. Through puberty, carnosine increased more in male subjects compared with female subjects, both in the gastrocnemius (+10.43% and -10.83%, respectively; interaction effect: P = 0.002) and in the soleus ( +24.30% and +5.49%, respectively; interaction effect: P = 0.012). No significant effect of puberty was found in either creatine (interaction effect: P = 0.307) or carnitine (interaction effect: P = 0.066). A sexual dimorphism in the adult human muscle is present in carnosine and creatine, but not in carnitine. NEW & NOTEWORTHY This is the first study to investigate sexual dimorphisms in skeletal muscle carnosine, creatine, and carnitine concentrations in a substantial adult reference population (n = 100). A sexual dimorphism is present in both carnosine and creatine at adult age. The origin of the sexual dimorphisms is investigated using a longitudinal design over puberty in 24 males and 24 females. The sexual dimorphism in carnosine originated partly during puberty for carnosine, but not for creatine.

The aim of this study was to identify markers of training stress and characteristics of middle-distance runners related to the incidence of overreaching following overload training. Twenty-four highly-trained runners (n=16 male; VO2peak=73.3(4.3) mL·kg·min-1; n=8 female, VO2peak=63.2(3.4) mL·kg·min-1) completed 3 weeks of normal training (NormTr), 3 weeks of high-volume training (HVTr; a 10, 20 and 30% increase in training volume each successive week from NormTr), and a 1-week taper (TapTr; 55% exponential reduction in training volume from HVTr week 3). Before, and immediately after each training period, an incremental treadmill-running test was performed, while resting metabolic rate (RMR), subjective fatigue responses and various resting blood biomarkers were assessed. Muscle fiber typology of the gastrocnemius was estimated by quantification of muscle carnosine using proton magnetic resonance spectroscopy and expressed as a z-score relative to a non-athlete control group. Twelve runners were classified as functionally overreached (FOR) following HVTr (decreased running TTE), whereas the other twelve were classified as acutely fatigued (AF; no decrease in running TTE). The FOR group did not demonstrate systematic alterations in RMR, resting blood biomarkers or submaximal exercise responses compared to the AF group. Gastrocnemius carnosine z-score was significantly higher in FOR (-0.44 ± 0.57) compared to AF (-1.25 ± 0.49, p = 0.004, d = 1.53) and was also associated with changes in running TTE from pre- to post-HVTr (r=-0.55, p=0.005) and pre-HVTr to post-TapTr (r=-0.64, p=0.008). Muscle fiber typology is related to the incidence of overreaching and performance super-compensation following increased training volume and a taper.

Purpose: To determine the influence of muscle fiber typology (MFT) on the pacing strategy of elite swimmers competing in the 200-m freestyle event. Method: The top 3 career-best performances from 25 elite 200-m freestyle swimmers were analyzed—12 women (1:58.0 [0:01.3] min:s) and 13 men (1:48.4 [0:02.5]). Muscle carnosine concentration was quantified by proton magnetic resonance spectroscopy in the gastrocnemius and soleus muscles and expressed as a carnosine aggregate z score (CAZ score) relative to an age- and gender-matched nonathlete control group to estimate MFT. Linear regression models were employed to examine the influence of MFT on the percentage of overall race time spent in each 50-m lap. Results: Swimmers with a higher CAZ score spent a greater percentage of race time in lap 3 compared with swimmers with a lower CAZ score (0.1%, 0.0% to 0.2%; mean, 90% confidence interval, P = .02). For every 1% increase in the percentage of race time spent in lap 1, the percentage of race time spent in lap 3 decreased by 0.4% for swimmers with a higher CAZ score (0.2% to −0.5%, P = .00, r = −.51), but not for swimmers with a lower CAZ score (−0.1%, −0.3% to 0.1%, P = .28, r = −.18). The percentage of race time spent in lap 4 decreased by 0.8% for higher-CAZ-score swimmers (−0.5% to −1.0%, P = .00, r = −.66) and by 0.9% for lower-CAZ-score swimmers (−0.6% to −1.3%, P = .00, r = −.65) when lap 1 percentage increased by 1%. Conclusion: MFT may influence the pacing strategy of swimmers in the 200-m freestyle event, which provides an avenue for maximizing individualized pacing strategies of elite swimmers.

Human fast-twitch muscle fibers generate high power in a short amount of time but are easily fatigued, whereas slow-twitch fibers are more fatigue resistant. The transfer of this knowledge to coaching is hampered by the invasive nature of the current evaluation of muscle typology by biopsies. Therefore, a noninvasive method was developed to estimate muscle typology through proton magnetic resonance spectroscopy in the gastrocnemius. The aim of this study was to investigate whether male subjects with an a priori-determined fast typology (FT) are characterized by a more pronounced Wingate exercise-induced fatigue and delayed recovery compared with subjects with a slow typology (ST). Ten subjects with an estimated higher percentage of fast-twitch fibers and 10 subjects with an estimated higher percentage of slow-twitch fibers underwent the test protocol, consisting of three 30-s all-out Wingate tests. Recovery of knee extension torque was evaluated by maximal voluntary contraction combined with electrical stimulation up to 5 h after the Wingate tests. Although both groups delivered the same mean power across all Wingates, the power drop was higher in the FT group (-61%) compared with the ST group (-41%). The torque at maximal voluntary contraction had fully recovered in the ST group after 20 min, whereas the FT group had not yet recovered 5 h into recovery. This noninvasive estimation of muscle typology can predict the extent of fatigue and time to recover following repeated all-out exercise and may have applications as a tool to individualize training and recovery cycles. NEW & NOTEWORTHY A one-fits-all training regime is present in most sports, though the same training implies different stimuli in athletes with a distinct muscle typology. Individualization of training based on this muscle typology might be important to optimize performance and to lower the risk for accumulated fatigue and potentially injury. When conducting research, one should keep in mind that the muscle typology of participants influences the severity of fatigue and might therefore impact the results., SCOPUS: ar.j, info:eu-repo/semantics/published