1. Proteolytic and nematicidal potential of the compost colonized by Hypsizygus marmoreus
- Author
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Elias Honorato Gomes, Luana Aparecida Simões Satiro, Frederico Vieira Froes, Bruna Leite Sufiate, Fabio Porto Sena, José Humberto de Queiroz, Vânia Mayumi Nakajima, Filippe Elias de Freitas Soares, and Fabio Ribeiro Braga
- Subjects
Proteases ,medicine.medical_treatment ,Immunology ,Complex Mixtures ,engineering.material ,Feces ,Rhabditida ,medicine ,Animals ,Zymography ,Food science ,Strongyloidea ,Residue (complex analysis) ,Protease ,Trichostrongyloidea ,biology ,Molecular mass ,Compost ,Composting ,Panagrellus redivivus ,fungi ,General Medicine ,biology.organism_classification ,Molecular Weight ,Infectious Diseases ,Larva ,engineering ,Cattle ,Electrophoresis, Polyacrylamide Gel ,Parasitology ,Spent mushroom compost ,Agaricales ,Peptide Hydrolases - Abstract
Spent mushroom compost (SMC) is a residue generated in edible mushrooms production, such as Hypsizygus marmoreus. Its genome was recently sequenced, demonstrating cuticle-degrading protease genes. The present work aims to investigate the proteases from H. marmoreus spent mushroom compost (SMC) by verifying its action on nematode larvae. The extraction of the crude extract directly with water from H. marmoreus SMC proved to be efficient for proteases obtainment, with proteolytic activity of 195.36 ± 18.38 U g−1 of compound. Moreover, the zymogram and SDS-PAGE indicated the presence of two proteases with estimated molecular weights of 30.2 and 33.7 kDa. Due to the protease activity present in H. marmoreus SMC extract, there was a significant reduction in the number of Panagrellus redivivus and L3 in treated group compared to control group (p
- Published
- 2019
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